Restriction map for
pFN21AE0031
(Circular) (Six-base) MAPSORT of: pFN21AE0031.seq Check: 1257 from: 1 to: 6187
pFN21AE0031 6187 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3997 278
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3066 3530 1937
Size: 1129 464 4594
Fragments arranged by size:
4594 1129 464
Acc65I G'GTAC_C
Cuts at: 3508 3508
Size: 6187
AclI AA'CG_TT
Cuts at: 1563 4314 4687 1563
Size: 2751 373 3063
Fragments arranged by size:
3063 2751 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3424 4250 5634 5902 1632
Size: 1792 826 1384 268 1917
Fragments arranged by size:
1917 1792 1384 826 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5990
AflIII A'CryG_T
Cuts at: 1203 5086 6063 1203
Size: 3883 977 1327
Fragments arranged by size:
3883 1327 977
AgeI A'CCGG_T
Cuts at: 5018 5018
Size: 6187
AhdI GACnn_n'nnGTC
Cuts at: 4916 4916
Size: 6187
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6187
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3269 3301 3269
Size: 32 6155
AlwNI CAG_nnn'CTG
Cuts at: 3930 5502 3930
Size: 1572 4615
ApaLI G'TGCA_C
Cuts at: 4244 5400 4244
Size: 1156 5031
ApoI r'AATT_y
Cuts at: 1493 2513 3466 3496 3722 3874 5983 1493
Size: 1020 953 30 226 152 2109 1697
Fragments arranged by size:
2109 1697 1020 953 226 152 30
AseI AT'TA_AT
Cuts at: 160 1032 4741 160
Size: 872 3709 1606
Fragments arranged by size:
3709 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
AvaI C'yCGr_G
Cuts at: 1870 1943 3512 1870
Size: 73 1569 4545
Fragments arranged by size:
4545 1569 73
BamHI G'GATC_C
Cuts at: 3517 3884 3517
Size: 367 5820
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3508 4963 618
Size: 325 217 627 1721 1455 1842
Fragments arranged by size:
1842 1721 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2371 3010 3506 1391
Size: 980 639 496 4072
Fragments arranged by size:
4072 980 639 496
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2955 928
Size: 971 1056 4160
Fragments arranged by size:
4160 1056 971
BbvCI CC'TCA_GC
Cuts at: 3258 3258
Size: 6187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2640 2674 4378 4412 1903
Size: 34 703 34 1704 34 3678
Fragments arranged by size:
3678 1704 703 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4079 5289 4079
Size: 1210 4977
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6187
BfrBI ATG'CAT
Cuts at: 2114 2114
Size: 6187
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4798 136
Size: 107 122 71 631 605 337 2789 1525
Fragments arranged by size:
2789 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 6182 6182
Size: 6187
BlpI GC'TnA_GC
Cuts at: 3604 3604
Size: 6187
Bme1580I G_kGCm'C
Cuts at: 1790 2509 4248 5404 1790
Size: 719 1739 1156 2573
Fragments arranged by size:
2573 1739 1156 719
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6187
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3918 4876 442
Size: 416 53 83 399 24 2501 958 1753
Fragments arranged by size:
2501 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2193 1056
Size: 1137 5050
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3024 3024
Size: 6187
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4847 1239
Size: 3608 2579
Bpu10I CC'TnA_GC
Cuts at: 2394 2924 3258 2394
Size: 530 334 5323
Fragments arranged by size:
5323 530 334
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2531 3019 4312 5177 5475 2531
Size: 488 1293 865 298 3243
Fragments arranged by size:
3243 1293 865 488 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4850 882
Size: 617 3351 2219
Fragments arranged by size:
3351 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3978 493
Size: 711 2774 2702
Fragments arranged by size:
2774 2702 711
BsaBI GATnn'nnATC
Cuts at: 3393 6181 3393
Size: 2788 3399
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3994 4376 5011 275
Size: 53 83 186 936 2461 382 635 1451
Fragments arranged by size:
2461 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4620 5018 5292 5439 5850 4620
Size: 398 274 147 411 4957
Fragments arranged by size:
4957 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3269 3299 1178
Size: 30 165 30 1866 30 4066
Fragments arranged by size:
4066 1866 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3287 3290 3383 1186
Size: 2101 3 93 3990
Fragments arranged by size:
3990 2101 93 3
BseYI C'CCAG_C
Cuts at: 2569 5390 5886 2569
Size: 2821 496 2870
Fragments arranged by size:
2870 2821 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2938 1163
Size: 573 1202 4412
Fragments arranged by size:
4412 1202 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 3637 4398 5426 1540
Size: 275 186 45 1591 761 1028 2301
Fragments arranged by size:
2301 1591 1028 761 275 186 45
BsiHKAI G_wGCw'C
Cuts at: 3010 4248 4333 5404 3010
Size: 1238 85 1071 3793
Fragments arranged by size:
3793 1238 1071 85
BsmI GAATG_Cn'
Cuts at: 2341 2422 3181 3416 3698 3791 2341
Size: 81 759 235 282 93 4737
Fragments arranged by size:
4737 759 282 235 93 81
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2371 2509 3010 3506 4248 4333 5404
Size: 399 581 138 501 496 742 85 1071
Cuts at: 5404 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 581 501 496 399 138
85
BspEI T'CCGG_A
Cuts at: 5850 5850
Size: 6187
BspHI T'CATG_A
Cuts at: 4076 5806 4076
Size: 1730 4457
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3542 844
Size: 2698 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4074 1394
Size: 429 2251 3507
Fragments arranged by size:
3507 2251 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2571 2745 4682 4856 65
Size: 1183 1323 174 1937 174 1396
Fragments arranged by size:
1937 1396 1323 1183 174 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4831 5018 1700
Size: 110 3021 187 2869
Fragments arranged by size:
3021 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2838 96
Size: 2742 3445
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6187
BssSI C'ACGA_G
Cuts at: 2732 4247 5259 2732
Size: 1515 1012 3660
Fragments arranged by size:
3660 1515 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2120 2751 794
Size: 1326 631 4230
Fragments arranged by size:
4230 1326 631
BstBI TT'CG_AA
Cuts at: 4991 4991
Size: 6187
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6187
BstYI r'GATC_y
Cuts at: 1972 3517 3884 4269 4286 5738 5824 5836 6182
Size: 1545 367 385 17 1452 86 12 346
Cuts at: 6182 1972
Size: 1977
Fragments arranged by size:
1977 1545 1452 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 3067 3067
Size: 6187
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2340 513
Size: 552 939 12 324 4360
Fragments arranged by size:
4360 939 552 324 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2040 2516 487
Size: 683 56 814 476 4158
Fragments arranged by size:
4158 814 683 476 56
BtsI GCAGTG_nn'
Cuts at: 2970 3703 4497 4517 2970
Size: 733 794 20 4640
Fragments arranged by size:
4640 794 733 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6187
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6152
DraI TTT'AAA
Cuts at: 3491 3491
Size: 6187
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2288 5194 809
Size: 507 972 2906 1802
Fragments arranged by size:
2906 1802 972 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3634 4523 6152
Size: 54 1425 50 275 18 1804 889 1629
Cuts at: 6152 8
Size: 43
Fragments arranged by size:
1804 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3634 1537
Size: 275 1822 4090
Fragments arranged by size:
4090 1822 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2072 2998 3125 4117 1353
Size: 719 926 127 992 3423
Fragments arranged by size:
3423 992 926 719 127
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4774 5148 5294 387
Size: 1064 138 3185 374 146 1280
Fragments arranged by size:
3185 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3008 3008
Size: 6187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3424 4250 4847 5634 5902 1239
Size: 393 1792 826 597 787 268 1524
Fragments arranged by size:
1792 1524 826 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 3365 3365
Size: 6187
EcoO109I rG'GnC_Cy
Cuts at: 3358 3358
Size: 6187
EcoRI G'AATT_C
Cuts at: 3496 3874 3496
Size: 378 5809
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3436 3436
Size: 6187
FspI TGC'GCA
Cuts at: 3114 4693 3114
Size: 1579 4608
HaeII r_GCGC'y
Cuts at: 1678 5334 1678
Size: 3656 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3214 3246 4828 4860 4902
Size: 32 135 32 1642 32 1582 32 42
Cuts at: 4902 4934 1373
Size: 32 2626
Fragments arranged by size:
2626 1642 1582 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3531 3777 669
Size: 988 281 1593 246 3079
Fragments arranged by size:
3079 1593 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2142 2921 2969 3201 748
Size: 1394 779 48 232 3734
Fragments arranged by size:
3734 1394 779 232 48
HpaI GTT'AAC
Cuts at: 3777 3777
Size: 6187
KpnI G_GTAC'C
Cuts at: 3512 3512
Size: 6187
MfeI C'AATT_G
Cuts at: 3786 3786
Size: 6187
MluI A'CGCG_T
Cuts at: 6063 6063
Size: 6187
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2256 3277 5301 5485 1305
Size: 951 1021 2024 184 2007
Fragments arranged by size:
2024 2007 1021 951 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4365
Fragments arranged by size:
4365 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2171 2219 2388 3380 4145 4504 4663
Size: 648 1005 48 169 992 765 359 159
Cuts at: 4663 518
Size: 2042
Fragments arranged by size:
2042 1005 992 765 648 359 169 159
48
MspA1I CmG'CkG
Cuts at: 1606 2018 3118 3603 4280 5428 5673 1606
Size: 412 1100 485 677 1148 245 2120
Fragments arranged by size:
2120 1148 1100 677 485 412 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2340 513
Size: 552 939 336 4360
Fragments arranged by size:
4360 939 552 336
NdeI CA'TA_TG
Cuts at: 387 2830 387
Size: 2443 3744
NheI G'CTAG_C
Cuts at: 1052 2189 1052
Size: 1137 5050
NotI GC'GGCC_GC
Cuts at: 3634 3634
Size: 6187
NsiI A_TGCA'T
Cuts at: 2116 2116
Size: 6187
NspI r_CATG'y
Cuts at: 1132 1229 2352 3545 5090 1132
Size: 97 1123 1193 1545 2229
Fragments arranged by size:
2229 1545 1193 1123 97
PasI CC'CwG_GG
Cuts at: 1348 2851 1348
Size: 1503 4684
PciI A'CATG_T
Cuts at: 5086 5086
Size: 6187
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6187
PfoI T'CCnGG_A
Cuts at: 2238 2238
Size: 6187
PmeI GTTT'AAAC
Cuts at: 3491 3491
Size: 6187
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3269 3301 4256 4288 5769 5801 3269
Size: 32 955 32 1481 32 3655
Fragments arranged by size:
3655 1481 955 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 3358 3358
Size: 6187
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6187
PsiI TTA'TAA
Cuts at: 2148 3757 2148
Size: 1609 4578
PstI C_TGCA'G
Cuts at: 830 2756 3539 830
Size: 1926 783 3478
Fragments arranged by size:
3478 1926 783
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6187
PvuII CAG'CTG
Cuts at: 3118 3118
Size: 6187
SacI G_AGCT'C
Cuts at: 3010 3010
Size: 6187
SacII CC_GC'GG
Cuts at: 2019 2019
Size: 6187
SalI G'TCGA_C
Cuts at: 1936 3529 1936
Size: 1593 4594
SapI GCTCTTCn'nnn_
Cuts at: 2998 2998
Size: 6187
SbfI CC_TGCA'GG
Cuts at: 3539 3539
Size: 6187
ScaI AGT'ACT
Cuts at: 4435 6147 4435
Size: 1712 4475
SfcI C'TryA_G
Cuts at: 826 1045 2752 3535 4670 5351 5542 826
Size: 219 1707 783 1135 681 191 1471
Fragments arranged by size:
1707 1471 1135 783 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6187
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6187
SmaI CCC'GGG
Cuts at: 1872 3514 1872
Size: 1642 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2546 2998 4291 5192 5454 820
Size: 197 926 603 452 1293 901 262 1553
Fragments arranged by size:
1553 1293 926 901 603 452 262 197
SnaBI TAC'GTA
Cuts at: 493 3978 493
Size: 3485 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6187
SphI G_CATG'C
Cuts at: 1132 3545 1132
Size: 2413 3774
SspI AAT'ATT
Cuts at: 5 52 4111 5
Size: 47 4059 2081
Fragments arranged by size:
4059 2081 47
StuI AGG'CCT
Cuts at: 1517 2741 3305 1517
Size: 1224 564 4399
Fragments arranged by size:
4399 1224 564
StyI C'CwwG_G
Cuts at: 513 1065 2004 2340 3626 513
Size: 552 939 336 1286 3074
Fragments arranged by size:
3074 1286 939 552 336
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3374 4214 4231 4384 4569 5014
Size: 1255 480 1314 840 17 153 185 445
Cuts at: 5014 325
Size: 1498
Fragments arranged by size:
1498 1314 1255 840 480 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2838 4433 6145
Size: 274 80 33 51 1443 861 1595 1712
Cuts at: 6145 96
Size: 138
Fragments arranged by size:
1712 1595 1443 861 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6147 6179 6147
Size: 32 6155
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6187
XbaI T'CTAG_A
Cuts at: 3523 3523
Size: 6187
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6187
XmaI C'CCGG_G
Cuts at: 1870 3512 1870
Size: 1642 4545
XmnI GAAnn'nnTTC
Cuts at: 2620 3284 4316 2620
Size: 664 1032 4491
Fragments arranged by size:
4491 1032 664
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3995 276
Size: 53 83 186 936 2461 2468
Fragments arranged by size:
2468 2461 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BaeI BbeI BsiWI
BsmBI BstXI Bsu36I DraIII EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI
PsrI RsrII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI