Restriction map for
pFN21AE0030
(Circular) (Six-base) MAPSORT of: pFN21AE0030.seq Check: 6628 from: 1 to: 6361
pFN21AE0030 6361 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2943 2943
Size: 6361
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2340 4171 278
Size: 53 83 186 936 804 1831 2468
Fragments arranged by size:
2468 1831 936 804 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3704 1937
Size: 1767 4594
Acc65I G'GTAC_C
Cuts at: 3682 3682
Size: 6361
AclI AA'CG_TT
Cuts at: 1563 4488 4861 1563
Size: 2925 373 3063
Fragments arranged by size:
3063 2925 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2030 2508 2858 3452 3521 3592 4424 5808
Size: 398 478 350 594 69 71 832 1384
Cuts at: 5808 6076 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 832 594 478 398 350 268
71 69
AfeI AGC'GCT
Cuts at: 2831 3092 2831
Size: 261 6100
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6164
AflIII A'CryG_T
Cuts at: 1203 2940 5260 6237 1203
Size: 1737 2320 977 1327
Fragments arranged by size:
2320 1737 1327 977
AgeI A'CCGG_T
Cuts at: 5192 5192
Size: 6361
AhdI GACnn_n'nnGTC
Cuts at: 3178 5090 3178
Size: 1912 4449
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6361
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2945 1184
Size: 1761 4600
AlwNI CAG_nnn'CTG
Cuts at: 3637 4104 5676 3637
Size: 467 1572 4322
Fragments arranged by size:
4322 1572 467
ApaLI G'TGCA_C
Cuts at: 3041 4418 5574 3041
Size: 1377 1156 3828
Fragments arranged by size:
3828 1377 1156
ApoI r'AATT_y
Cuts at: 1493 3670 3896 4048 6157 1493
Size: 2177 226 152 2109 1697
Fragments arranged by size:
2177 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4915 160
Size: 872 3883 1606
Fragments arranged by size:
3883 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
AvaI C'yCGr_G
Cuts at: 1870 1943 2955 3183 3240 3686 1870
Size: 73 1012 228 57 446 4545
Fragments arranged by size:
4545 1012 446 228 73 57
BamHI G'GATC_C
Cuts at: 3691 4058 3691
Size: 367 5994
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2071 2288 2963 3682 5137
Size: 325 217 627 284 217 675 719 1455
Cuts at: 5137 618
Size: 1842
Fragments arranged by size:
1842 1455 719 675 627 325 284 217
217
BanII G_rGCy'C
Cuts at: 1391 2474 3680 1391
Size: 1083 1206 4072
Fragments arranged by size:
4072 1206 1083
BbeI G_GCGC'C
Cuts at: 2075 2292 2075
Size: 217 6144
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2035 2847 3478 928
Size: 971 136 812 631 3811
Fragments arranged by size:
3811 971 812 631 136
BbvCI CC'TCA_GC
Cuts at: 3398 3398
Size: 6361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4552 4586 1903
Size: 34 50 34 2531 34 3678
Fragments arranged by size:
3678 2531 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4253 5463 4253
Size: 1210 5151
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6361
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3593 4972 136
Size: 107 122 71 631 605 1921 1379 1525
Fragments arranged by size:
1921 1525 1379 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6356 6356
Size: 6361
BlpI GC'TnA_GC
Cuts at: 2210 3778 2210
Size: 1568 4793
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2966 3045 4422 5578 1790
Size: 541 635 79 1377 1156 2573
Fragments arranged by size:
2573 1377 1156 635 541 79
BmgBI CAC'GTC
Cuts at: 1318 3070 3175 3439 3454 1318
Size: 1752 105 264 15 4225
Fragments arranged by size:
4225 1752 264 105 15
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4092 5050 442
Size: 416 53 83 399 24 2675 958 1753
Fragments arranged by size:
2675 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6361
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2738 2817 2848 3014 3155 3428 5021 1239
Size: 1499 79 31 166 141 273 1593 2579
Fragments arranged by size:
2579 1593 1499 273 166 141 79 31
Bpu10I CC'TnA_GC
Cuts at: 3398 3419 3398
Size: 21 6340
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4486 5351 5649 4486
Size: 865 298 5198
Fragments arranged by size:
5198 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2378 5024 882
Size: 617 879 2646 2219
Fragments arranged by size:
2646 2219 879 617
BsaAI yAC'GTr
Cuts at: 493 1204 2941 3592 4152 493
Size: 711 1737 651 560 2702
Fragments arranged by size:
2702 1737 711 651 560
BsaBI GATnn'nnATC
Cuts at: 3308 6355 3308
Size: 3047 3314
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2015 2072 2094 2289
Size: 53 83 186 936 482 57 22 195
Cuts at: 2289 2337 2649 2703 4168 4550 5185 275
Size: 48 312 54 1465 382 635 1451
Fragments arranged by size:
1465 1451 936 635 482 382 312 195
186 83 57 54 53 48 22
BsaWI w'CCGG_w
Cuts at: 4794 5192 5466 5613 6024 4794
Size: 398 274 147 411 5131
Fragments arranged by size:
5131 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2846 2876 1178
Size: 30 165 30 1443 30 4663
Fragments arranged by size:
4663 1443 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2814 3605 1186
Size: 1628 791 3942
Fragments arranged by size:
3942 1628 791
BseYI C'CCAG_C
Cuts at: 2602 2677 5564 6060 2602
Size: 75 2887 496 2903
Fragments arranged by size:
2903 2887 496 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2915 2921 1163
Size: 573 1179 6 4603
Fragments arranged by size:
4603 1179 573 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3537 3811 4572 5600 1540
Size: 275 186 1536 274 761 1028 2301
Fragments arranged by size:
2301 1536 1028 761 275 274 186
BsiHKAI G_wGCw'C
Cuts at: 2229 3045 3087 3414 4422 4507 5578 2229
Size: 816 42 327 1008 85 1071 3012
Fragments arranged by size:
3012 1071 1008 816 327 85 42
BsmI GAATG_Cn'
Cuts at: 2170 3520 3872 3965 2170
Size: 1350 352 93 4566
Fragments arranged by size:
4566 1350 352 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2710 2990 2710
Size: 280 6081
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2229 2331 2474 2966 3045 3087 3414
Size: 399 439 102 143 492 79 42 327
Cuts at: 3414 3680 4422 4507 5578 1391
Size: 266 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 492 439 399 327 266
143 102 85 79 42
BspEI T'CCGG_A
Cuts at: 6024 6024
Size: 6361
BspHI T'CATG_A
Cuts at: 4250 5980 4250
Size: 1730 4631
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2145 2943 3555 3716 844
Size: 1301 798 612 161 3489
Fragments arranged by size:
3489 1301 798 612 161
BsrBI CCG'CTC
Cuts at: 1394 1823 2700 4248 1394
Size: 429 877 1548 3507
Fragments arranged by size:
3507 1548 877 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3222 3564 4856 5030 65
Size: 1183 1974 342 1292 174 1396
Fragments arranged by size:
1974 1396 1292 1183 342 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3286 3532 5005 5192 1700
Size: 110 1476 246 1473 187 2869
Fragments arranged by size:
2869 1476 1473 246 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6361
BssHII G'CGCG_C
Cuts at: 1925 2186 1925
Size: 261 6100
BssSI C'ACGA_G
Cuts at: 2102 3086 3214 3641 4421 5433 2102
Size: 984 128 427 780 1012 3030
Fragments arranged by size:
3030 1012 984 780 427 128
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6361
BstBI TT'CG_AA
Cuts at: 5165 5165
Size: 6361
BstEII G'GTnAC_C
Cuts at: 1184 2530 1184
Size: 1346 5015
BstXI CCAn_nnnn'nTGG
Cuts at: 2317 2317
Size: 6361
BstYI r'GATC_y
Cuts at: 1972 3309 3691 4058 4443 4460 5912 5998 6010
Size: 1337 382 367 385 17 1452 86 12
Cuts at: 6010 6356 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1337 385 382 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3021 3021
Size: 6361
BtgI C'CryG_G
Cuts at: 513 1065 2292 2316 2379 2409 2910 3036 3359
Size: 552 1227 24 63 30 501 126 323
Cuts at: 3359 513
Size: 3515
Fragments arranged by size:
3515 1227 552 501 323 126 63 30
24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5622
Fragments arranged by size:
5622 683 56
BtsI GCAGTG_nn'
Cuts at: 2175 2508 3877 4671 4691 2175
Size: 333 1369 794 20 3845
Fragments arranged by size:
3845 1369 794 333 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6361
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6326
DraI TTT'AAA
Cuts at: 3665 3665
Size: 6361
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5368 809
Size: 507 4052 1802
Fragments arranged by size:
4052 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2319 2376 2505
Size: 54 1425 50 275 18 489 57 129
Cuts at: 2505 3003 3534 3808 4697 6326 8
Size: 498 531 274 889 1629 43
Fragments arranged by size:
1629 1425 889 531 498 489 275 274
129 57 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3534 3808 1537
Size: 275 1722 274 4090
Fragments arranged by size:
4090 1722 275 274
EarI CTCTTCn'nnn_
Cuts at: 1353 2738 4291 1353
Size: 1385 1553 3423
Fragments arranged by size:
3423 1553 1385
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2216 2449 2464 2622 3077 3092
Size: 1064 138 627 233 15 158 455 15
Cuts at: 3092 4948 5322 5468 387
Size: 1856 374 146 1280
Fragments arranged by size:
1856 1280 1064 627 455 374 233 158
146 138 15 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2030 2508 2738 2817 2848 2858 3014
Size: 393 398 478 230 79 31 10 156
Cuts at: 3014 3155 3428 3452 3521 3592 4424 5021 5808
Size: 141 273 24 69 71 832 597 787
Cuts at: 5808 6076 1239
Size: 268 1524
Fragments arranged by size:
1524 832 787 597 478 398 393 273
268 230 156 141 79 71 69 31
24 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2873 3062 2873
Size: 189 6172
EcoO109I rG'GnC_Cy
Cuts at: 2269 2422 2614 3232 3472 2269
Size: 153 192 618 240 5158
Fragments arranged by size:
5158 618 240 192 153
EcoRI G'AATT_C
Cuts at: 3670 4048 3670
Size: 378 5983
FspI TGC'GCA
Cuts at: 2501 4867 2501
Size: 2366 3995
HaeII r_GCGC'y
Cuts at: 1678 2075 2292 2833 3094 5508 1678
Size: 397 217 541 261 2414 2531
Fragments arranged by size:
2531 2414 541 397 261 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3164 3196 5002 5034 5076
Size: 32 135 32 1592 32 1806 32 42
Cuts at: 5076 5108 1373
Size: 32 2626
Fragments arranged by size:
2626 1806 1592 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3705 3951 669
Size: 988 281 1767 246 3079
Fragments arranged by size:
3079 1767 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6361
HpaI GTT'AAC
Cuts at: 3951 3951
Size: 6361
KasI G'GCGC_C
Cuts at: 2071 2288 2071
Size: 217 6144
KpnI G_GTAC'C
Cuts at: 3686 3686
Size: 6361
MfeI C'AATT_G
Cuts at: 3960 3960
Size: 6361
MluI A'CGCG_T
Cuts at: 6237 6237
Size: 6361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2715 5475 5659 1305
Size: 1410 2760 184 2007
Fragments arranged by size:
2760 2007 1410 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2378 10
Size: 54 1425 343 546 3993
Fragments arranged by size:
3993 1425 546 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3173 3287 4319 4678 4837 518
Size: 648 2007 114 1032 359 159 2042
Fragments arranged by size:
2042 2007 1032 648 359 159 114
MspA1I CmG'CkG
Cuts at: 1606 2054 2080 2220 2538 3325 3361 3373 3499
Size: 448 26 140 318 787 36 12 126
Cuts at: 3499 3777 4454 5602 5847 1606
Size: 278 677 1148 245 2120
Fragments arranged by size:
2120 1148 787 677 448 318 278 245
140 126 36 26 12
NaeI GCC'GGC
Cuts at: 3534 3534
Size: 6361
NarI GG'CG_CC
Cuts at: 2072 2289 2072
Size: 217 6144
NcoI C'CATG_G
Cuts at: 513 1065 2316 2379 3036 513
Size: 552 1251 63 657 3838
Fragments arranged by size:
3838 1251 657 552 63
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6361
NgoMIV G'CCGG_C
Cuts at: 3532 3532
Size: 6361
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6361
NotI GC'GGCC_GC
Cuts at: 3808 3808
Size: 6361
NspI r_CATG'y
Cuts at: 1132 1229 2125 3286 3719 5264 1132
Size: 97 896 1161 433 1545 2229
Fragments arranged by size:
2229 1545 1161 896 433 97
PasI CC'CwG_GG
Cuts at: 1348 2310 2467 1348
Size: 962 157 5242
Fragments arranged by size:
5242 962 157
PciI A'CATG_T
Cuts at: 5260 5260
Size: 6361
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2832 1108
Size: 1724 4637
PmeI GTTT'AAAC
Cuts at: 3665 3665
Size: 6361
PmlI CAC'GTG
Cuts at: 2941 2941
Size: 6361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3014 3046 4430 4462 5943 5975 3014
Size: 32 1384 32 1481 32 3400
Fragments arranged by size:
3400 1481 1384 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2614 3472 2614
Size: 858 5503
PshAI GACnn'nnGTC
Cuts at: 1613 2390 3242 1613
Size: 777 852 4732
Fragments arranged by size:
4732 852 777
PsiI TTA'TAA
Cuts at: 3931 3931
Size: 6361
PspXI vC'TCGA_Gb
Cuts at: 3240 3240
Size: 6361
PstI C_TGCA'G
Cuts at: 830 2142 2973 3713 830
Size: 1312 831 740 3478
Fragments arranged by size:
3478 1312 831 740
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6361
PvuII CAG'CTG
Cuts at: 3325 3373 3499 3325
Size: 48 126 6187
Fragments arranged by size:
6187 126 48
SacII CC_GC'GG
Cuts at: 3362 3362
Size: 6361
SalI G'TCGA_C
Cuts at: 1936 3703 1936
Size: 1767 4594
SanDI GG'GwC_CC
Cuts at: 3472 3472
Size: 6361
SbfI CC_TGCA'GG
Cuts at: 2142 3713 2142
Size: 1571 4790
ScaI AGT'ACT
Cuts at: 2493 4609 6321 2493
Size: 2116 1712 2533
Fragments arranged by size:
2533 2116 1712
SfcI C'TryA_G
Cuts at: 826 1045 2138 2969 3709 4844 5525 5716 826
Size: 219 1093 831 740 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 831 740 681 219 191
SfoI GGC'GCC
Cuts at: 2073 2290 2073
Size: 217 6144
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6361
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6361
SmaI CCC'GGG
Cuts at: 1872 3185 3688 1872
Size: 1313 503 4545
Fragments arranged by size:
4545 1313 503
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3240 4465 5366 5628 820
Size: 197 926 1297 1225 901 262 1553
Fragments arranged by size:
1553 1297 1225 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4152 493
Size: 3659 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6361
SphI G_CATG'C
Cuts at: 1132 2125 3719 1132
Size: 993 1594 3774
Fragments arranged by size:
3774 1594 993
SspI AAT'ATT
Cuts at: 5 52 4285 5
Size: 47 4233 2081
Fragments arranged by size:
4233 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6361
StyI C'CwwG_G
Cuts at: 513 1065 2316 2379 3036 3800 513
Size: 552 1251 63 657 764 3074
Fragments arranged by size:
3074 1251 764 657 552 63
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4388 4405 4558 4743 5188 325
Size: 1255 2808 17 153 185 445 1498
Fragments arranged by size:
2808 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2400 2491 4607
Size: 274 80 33 51 1443 423 91 2116
Cuts at: 4607 6319 96
Size: 1712 138
Fragments arranged by size:
2116 1712 1443 423 274 138 91 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2846 2878 6321 6353 2846
Size: 32 3443 32 2854
Fragments arranged by size:
3443 2854 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2339 1316
Size: 1023 5338
XbaI T'CTAG_A
Cuts at: 3697 3697
Size: 6361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2632 2833 2632
Size: 201 6160
XhoI C'TCGA_G
Cuts at: 1943 3240 1943
Size: 1297 5064
XmaI C'CCGG_G
Cuts at: 1870 3183 3686 1870
Size: 1313 503 4545
Fragments arranged by size:
4545 1313 503
XmnI GAAnn'nnTTC
Cuts at: 4490 4490
Size: 6361
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2338 4169 276
Size: 53 83 186 936 804 1831 2468
Fragments arranged by size:
2468 1831 936 804 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PstI PvuI PvuII SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI
BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NruI
NsiI PacI PfoI PspOMI PsrI RsrII SacI SapI
SexAI SfiI SrfI SwaI