Restriction map for
pFN21AE0029
(Circular) (Six-base) MAPSORT of: pFN21AE0029.seq Check: 1867 from: 1 to: 6295
pFN21AE0029 6295 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4105 278
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3638 1937
Size: 1701 4594
Acc65I G'GTAC_C
Cuts at: 3616 3616
Size: 6295
AclI AA'CG_TT
Cuts at: 1563 4422 4795 1563
Size: 2859 373 3063
Fragments arranged by size:
3063 2859 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4358 5742 6010 1632
Size: 2726 1384 268 1917
Fragments arranged by size:
2726 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2216 3127 3331 820
Size: 197 1199 911 204 3784
Fragments arranged by size:
3784 1199 911 204 197
AflIII A'CryG_T
Cuts at: 1203 5194 6171 1203
Size: 3991 977 1327
Fragments arranged by size:
3991 1327 977
AgeI A'CCGG_T
Cuts at: 2532 5126 2532
Size: 2594 3701
AhdI GACnn_n'nnGTC
Cuts at: 5024 5024
Size: 6295
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6295
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6295
AlwNI CAG_nnn'CTG
Cuts at: 3176 4038 5610 3176
Size: 862 1572 3861
Fragments arranged by size:
3861 1572 862
ApaLI G'TGCA_C
Cuts at: 4352 5508 4352
Size: 1156 5139
ApoI r'AATT_y
Cuts at: 1493 2048 2325 2708 3604 3830 3982 6091 1493
Size: 555 277 383 896 226 152 2109 1697
Fragments arranged by size:
2109 1697 896 555 383 277 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4849 160
Size: 872 3817 1606
Fragments arranged by size:
3817 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
AvaI C'yCGr_G
Cuts at: 1870 1943 3201 3620 1870
Size: 73 1258 419 4545
Fragments arranged by size:
4545 1258 419 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2100 2133 2100
Size: 33 6262
BamHI G'GATC_C
Cuts at: 2432 2701 3625 3992 2432
Size: 269 924 367 4735
Fragments arranged by size:
4735 924 367 269
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3313 3616 5071 618
Size: 325 217 627 1526 303 1455 1842
Fragments arranged by size:
1842 1526 1455 627 325 303 217
BanII G_rGCy'C
Cuts at: 1391 2160 2193 2806 3200 3522 3538 3614 1391
Size: 769 33 613 394 322 16 76 4072
Fragments arranged by size:
4072 769 613 394 322 76 33 16
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3492 928
Size: 971 1593 3731
Fragments arranged by size:
3731 1593 971
BbvCI CC'TCA_GC
Cuts at: 3000 3074 3524 3000
Size: 74 450 5771
Fragments arranged by size:
5771 450 74
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4486 4520 1903
Size: 34 50 34 2465 34 3678
Fragments arranged by size:
3678 2465 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4187 5397 4187
Size: 1210 5085
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6295
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4906 136
Size: 107 122 71 631 605 3234 1525
Fragments arranged by size:
3234 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6290 6290
Size: 6295
BlpI GC'TnA_GC
Cuts at: 3712 3712
Size: 6295
Bme1580I G_kGCm'C
Cuts at: 1790 4356 5512 1790
Size: 2566 1156 2573
Fragments arranged by size:
2573 2566 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6295
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4026 4984
Size: 416 53 83 399 24 1736 873 958
Cuts at: 4984 442
Size: 1753
Fragments arranged by size:
1753 1736 958 873 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6295
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2958 2958
Size: 6295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2183 4955 1239
Size: 944 2772 2579
Fragments arranged by size:
2772 2579 944
Bpu10I CC'TnA_GC
Cuts at: 3000 3074 3290 3524 3000
Size: 74 216 234 5771
Fragments arranged by size:
5771 234 216 74
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3346 4420 5285 5583 3346
Size: 1074 865 298 4058
Fragments arranged by size:
4058 1074 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4958 882
Size: 617 3459 2219
Fragments arranged by size:
3459 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4086 493
Size: 711 2882 2702
Fragments arranged by size:
2882 2702 711
BsaBI GATnn'nnATC
Cuts at: 2499 3058 6289 2499
Size: 559 3231 2505
Fragments arranged by size:
3231 2505 559
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4102 4484 5119 275
Size: 53 83 186 936 2569 382 635 1451
Fragments arranged by size:
2569 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2100 2532 3342 4728 5126 5400 5547 5958 2100
Size: 432 810 1386 398 274 147 411 2437
Fragments arranged by size:
2437 1386 810 432 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6070
Fragments arranged by size:
6070 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3030 3035 3097 3512 1186
Size: 1844 5 62 415 3969
Fragments arranged by size:
3969 1844 415 62 5
BseYI C'CCAG_C
Cuts at: 2008 2805 5498 5994 2008
Size: 797 2693 496 2309
Fragments arranged by size:
2693 2309 797 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2508 1163
Size: 573 772 4950
Fragments arranged by size:
4950 772 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3745 4506 5534 1540
Size: 275 186 1744 761 1028 2301
Fragments arranged by size:
2301 1744 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2160 2193 3522 4356 4441 5512 2160
Size: 33 1329 834 85 1071 2943
Fragments arranged by size:
2943 1329 1071 834 85 33
BsmI GAATG_Cn'
Cuts at: 3806 3899 3806
Size: 93 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2193 2806 3200 3522 3538 3614
Size: 399 370 33 613 394 322 16 76
Cuts at: 3614 4356 4441 5512 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 613 399 394 370 322
85 76 33 16
BspEI T'CCGG_A
Cuts at: 3342 5958 3342
Size: 2616 3679
BspHI T'CATG_A
Cuts at: 4184 5914 4184
Size: 1730 4565
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3650 844
Size: 2806 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4182 1394
Size: 429 2359 3507
Fragments arranged by size:
3507 2359 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4790 4964 65
Size: 1183 3542 174 1396
Fragments arranged by size:
3542 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2532 3489 4939 5126 1700
Size: 110 722 957 1450 187 2869
Fragments arranged by size:
2869 1450 957 722 187 110
BsrGI T'GTAC_A
Cuts at: 96 3229 96
Size: 3133 3162
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6295
BssSI C'ACGA_G
Cuts at: 4355 5367 4355
Size: 1012 5283
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2239 794
Size: 1445 4850
BstBI TT'CG_AA
Cuts at: 5099 5099
Size: 6295
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6295
BstYI r'GATC_y
Cuts at: 1972 2432 2701 3625 3992 4377 4394 5846 5932
Size: 460 269 924 367 385 17 1452 86
Cuts at: 5932 5944 6290 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 924 460 385 367 346 269
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2436 2651 513
Size: 552 1371 215 4157
Fragments arranged by size:
4157 1371 552 215
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5556
Fragments arranged by size:
5556 683 56
BtsI GCAGTG_nn'
Cuts at: 2228 2520 3811 4605 4625 2228
Size: 292 1291 794 20 3898
Fragments arranged by size:
3898 1291 794 292 20
ClaI AT'CG_AT
Cuts at: 1554 3460 1554
Size: 1906 4389
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6260
DraI TTT'AAA
Cuts at: 3599 3599
Size: 6295
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3550 5302 809
Size: 507 2234 1752 1802
Fragments arranged by size:
2234 1802 1752 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2770 3304 3742
Size: 54 1425 50 275 18 940 534 438
Cuts at: 3742 4631 6260 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 940 889 534 438 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3742 1537
Size: 275 1930 4090
Fragments arranged by size:
4090 1930 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2983 3115 4225 1353
Size: 1630 132 1110 3423
Fragments arranged by size:
3423 1630 1110 132
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4882 5256 5402 387
Size: 1064 138 3293 374 146 1280
Fragments arranged by size:
3293 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2158 2191 3520 2158
Size: 33 1329 4933
Fragments arranged by size:
4933 1329 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2183 4358 4955 5742 6010 1239
Size: 393 551 2175 597 787 268 1524
Fragments arranged by size:
2175 1524 787 597 551 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2771 2771
Size: 6295
EcoO109I rG'GnC_Cy
Cuts at: 2764 2764
Size: 6295
EcoRI G'AATT_C
Cuts at: 2048 3604 3982 2048
Size: 1556 378 4361
Fragments arranged by size:
4361 1556 378
EcoRV GAT'ATC
Cuts at: 2791 2791
Size: 6295
FspI TGC'GCA
Cuts at: 4801 4801
Size: 6295
HaeII r_GCGC'y
Cuts at: 1678 5442 1678
Size: 3764 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4936 4968 5010 5042 1373
Size: 32 135 32 3364 32 42 32 2626
Fragments arranged by size:
3364 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3639 3885 669
Size: 988 281 1701 246 3079
Fragments arranged by size:
3079 1701 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3124 748
Size: 2376 3919
HpaI GTT'AAC
Cuts at: 3885 3885
Size: 6295
KpnI G_GTAC'C
Cuts at: 3620 3620
Size: 6295
MfeI C'AATT_G
Cuts at: 2427 3087 3894 2427
Size: 660 807 4828
Fragments arranged by size:
4828 807 660
MluI A'CGCG_T
Cuts at: 6171 6171
Size: 6295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2597 2948 5409 5593 1305
Size: 1292 351 2461 184 2007
Fragments arranged by size:
2461 2007 1292 351 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2772 3306 10
Size: 54 1425 343 940 534 2999
Fragments arranged by size:
2999 1425 940 534 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4253 4612 4771 518
Size: 648 3087 359 159 2042
Fragments arranged by size:
3087 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2182 3711 4388 5536 5781 1606
Size: 576 1529 677 1148 245 2120
Fragments arranged by size:
2120 1529 1148 677 576 245
NcoI C'CATG_G
Cuts at: 513 1065 2436 513
Size: 552 1371 4372
Fragments arranged by size:
4372 1371 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6295
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6295
NotI GC'GGCC_GC
Cuts at: 3742 3742
Size: 6295
NspI r_CATG'y
Cuts at: 1132 1229 3653 5198 1132
Size: 97 2424 1545 2229
Fragments arranged by size:
2424 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6295
PciI A'CATG_T
Cuts at: 5194 5194
Size: 6295
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6295
PfoI T'CCnGG_A
Cuts at: 2160 2160
Size: 6295
PmeI GTTT'AAAC
Cuts at: 3599 3599
Size: 6295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4364 4396 5877 5909 4364
Size: 32 1481 32 4750
Fragments arranged by size:
4750 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2764 2764
Size: 6295
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6295
PsiI TTA'TAA
Cuts at: 2568 3865 2568
Size: 1297 4998
PstI C_TGCA'G
Cuts at: 830 2235 3483 3647 830
Size: 1405 1248 164 3478
Fragments arranged by size:
3478 1405 1248 164
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6295
PvuII CAG'CTG
Cuts at: 2182 2182
Size: 6295
SacI G_AGCT'C
Cuts at: 2160 2193 3522 2160
Size: 33 1329 4933
Fragments arranged by size:
4933 1329 33
SalI G'TCGA_C
Cuts at: 1936 3637 1936
Size: 1701 4594
SbfI CC_TGCA'GG
Cuts at: 3647 3647
Size: 6295
ScaI AGT'ACT
Cuts at: 4543 6255 4543
Size: 1712 4583
SfcI C'TryA_G
Cuts at: 826 1045 2231 3479 3643 4778 5459 5650 826
Size: 219 1186 1248 164 1135 681 191 1471
Fragments arranged by size:
1471 1248 1186 1135 681 219 191 164
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6295
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6295
SmaI CCC'GGG
Cuts at: 1872 3622 1872
Size: 1750 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2216 3127 3201 3331 3361 4399
Size: 197 926 273 911 74 130 30 1038
Cuts at: 4399 5300 5562 820
Size: 901 262 1553
Fragments arranged by size:
1553 1038 926 911 901 273 262 197
130 74 30
SnaBI TAC'GTA
Cuts at: 493 4086 493
Size: 3593 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6295
SphI G_CATG'C
Cuts at: 1132 3653 1132
Size: 2521 3774
SspI AAT'ATT
Cuts at: 5 52 4219 5
Size: 47 4167 2081
Fragments arranged by size:
4167 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6295
StyI C'CwwG_G
Cuts at: 513 1065 2436 2767 3156 3734 513
Size: 552 1371 331 389 578 3074
Fragments arranged by size:
3074 1371 578 552 389 331
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4322 4339 4492 4677 5122 325
Size: 1255 2742 17 153 185 445 1498
Fragments arranged by size:
2742 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3229 4541 6253
Size: 274 80 33 51 1443 1252 1312 1712
Cuts at: 6253 96
Size: 138
Fragments arranged by size:
1712 1443 1312 1252 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3337 3369 6255 6287 3337
Size: 32 2886 32 3345
Fragments arranged by size:
3345 2886 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6295
XbaI T'CTAG_A
Cuts at: 3631 3631
Size: 6295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2250 2250
Size: 6295
XhoI C'TCGA_G
Cuts at: 1943 3201 1943
Size: 1258 5037
XmaI C'CCGG_G
Cuts at: 1870 3620 1870
Size: 1750 4545
XmnI GAAnn'nnTTC
Cuts at: 2313 4424 2313
Size: 2111 4184
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4103 276
Size: 53 83 186 936 2569 2468
Fragments arranged by size:
2569 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI