Restriction map for
pFN21AE0028
(Circular) (Six-base) MAPSORT of: pFN21AE0028.seq Check: 1647 from: 1 to: 6946
pFN21AE0028 6946 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2155 2155
Size: 6946
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2921 4756 278
Size: 53 83 186 936 1385 1835 2468
Fragments arranged by size:
2468 1835 1385 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2295 3825 4289 1937
Size: 358 1530 464 4594
Fragments arranged by size:
4594 1530 464 358
Acc65I G'GTAC_C
Cuts at: 4267 4267
Size: 6946
AclI AA'CG_TT
Cuts at: 1563 5073 5446 1563
Size: 3510 373 3063
Fragments arranged by size:
3510 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2206 2651 5009 6393 6661 1632
Size: 574 445 2358 1384 268 1917
Fragments arranged by size:
2358 1917 1384 574 445 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6749
AflIII A'CryG_T
Cuts at: 1203 4052 5845 6822 1203
Size: 2849 1793 977 1327
Fragments arranged by size:
2849 1793 1327 977
AgeI A'CCGG_T
Cuts at: 5777 5777
Size: 6946
AhdI GACnn_n'nnGTC
Cuts at: 2917 5675 2917
Size: 2758 4188
AleI CACnn'nnGTG
Cuts at: 1166 3909 1166
Size: 2743 4203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3647 1184
Size: 2463 4483
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 3869 3901 2105
Size: 32 1732 32 5150
Fragments arranged by size:
5150 1732 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2522 3092 3158 4689 6261 2522
Size: 570 66 1531 1572 3207
Fragments arranged by size:
3207 1572 1531 570 66
ApaI G_GGCC'C
Cuts at: 2849 3811 3959 2849
Size: 962 148 5836
Fragments arranged by size:
5836 962 148
ApaLI G'TGCA_C
Cuts at: 5003 6159 5003
Size: 1156 5790
ApoI r'AATT_y
Cuts at: 1493 4255 4481 4633 6742 1493
Size: 2762 226 152 2109 1697
Fragments arranged by size:
2762 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5500 160
Size: 872 4468 1606
Fragments arranged by size:
4468 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
AvaI C'yCGr_G
Cuts at: 1870 1943 3138 3179 4271 1870
Size: 73 1195 41 1092 4545
Fragments arranged by size:
4545 1195 1092 73 41
AvrII C'CTAG_G
Cuts at: 4123 4123
Size: 6946
BamHI G'GATC_C
Cuts at: 3679 4276 4643 3679
Size: 597 367 5982
Fragments arranged by size:
5982 597 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2897 2997 4267 5722 618
Size: 325 217 627 1110 100 1270 1455 1842
Fragments arranged by size:
1842 1455 1270 1110 627 325 217 100
BanII G_rGCy'C
Cuts at: 1391 2822 2849 2867 3099 3111 3811 3959 4141
Size: 1431 27 18 232 12 700 148 182
Cuts at: 4141 4265 1391
Size: 124 4072
Fragments arranged by size:
4072 1431 700 232 182 148 124 27
18 12
BbeI G_GCGC'C
Cuts at: 3001 3001
Size: 6946
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5975
BbvCI CC'TCA_GC
Cuts at: 2948 2948
Size: 6946
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5137 5171 1903
Size: 34 3200 34 3678
Fragments arranged by size:
3678 3200 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3934 3968 4838 6048 3934
Size: 34 870 1210 4832
Fragments arranged by size:
4832 1210 870 34
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6946
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4128 5557 136
Size: 107 122 71 631 605 2456 1429 1525
Fragments arranged by size:
2456 1525 1429 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6941 6941
Size: 6946
BlpI GC'TnA_GC
Cuts at: 3093 4363 3093
Size: 1270 5676
Bme1580I G_kGCm'C
Cuts at: 1790 2849 2900 3811 3959 5007 6163 1790
Size: 1059 51 911 148 1048 1156 2573
Fragments arranged by size:
2573 1156 1059 1048 911 148 51
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6946
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2761 2973 3046
Size: 416 53 83 399 24 1344 212 73
Cuts at: 3046 4677 5635 442
Size: 1631 958 1753
Fragments arranged by size:
1753 1631 1344 958 416 399 212 83
73 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6946
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3052 3844 3052
Size: 792 6154
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2345 3650 3823 4127 4180 5606 1239
Size: 1106 1305 173 304 53 1426 2579
Fragments arranged by size:
2579 1426 1305 1106 304 173 53
Bpu10I CC'TnA_GC
Cuts at: 2328 2492 2841 2948 2328
Size: 164 349 107 6326
Fragments arranged by size:
6326 349 164 107
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2809 3902 4138 5071 5936 6234 2809
Size: 1093 236 933 865 298 3521
Fragments arranged by size:
3521 1093 933 865 298 236
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5609 882
Size: 617 4110 2219
Fragments arranged by size:
4110 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4737 493
Size: 711 3533 2702
Fragments arranged by size:
3533 2702 711
BsaBI GATnn'nnATC
Cuts at: 6940 6940
Size: 6946
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2091 2918 2998 3756
Size: 53 83 186 936 558 827 80 758
Cuts at: 3756 4071 4753 5135 5770 275
Size: 315 682 382 635 1451
Fragments arranged by size:
1451 936 827 758 682 635 558 382
315 186 83 80 53
BsaWI w'CCGG_w
Cuts at: 2921 2972 5379 5777 6051 6198 6609 2921
Size: 51 2407 398 274 147 411 3258
Fragments arranged by size:
3258 2407 411 398 274 147 51
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2105 2135 1178
Size: 30 165 30 702 30 5989
Fragments arranged by size:
5989 702 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2654 2841 2936 3183 3224 3280 1186
Size: 1468 187 95 247 41 56 4852
Fragments arranged by size:
4852 1468 247 187 95 56 41
BseYI C'CCAG_C
Cuts at: 3199 3406 4084 6149 6645 3199
Size: 207 678 2065 496 3500
Fragments arranged by size:
3500 2065 678 496 207
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2465 2761 3078 3532 1163
Size: 573 729 296 317 454 4577
Fragments arranged by size:
4577 729 573 454 317 296
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4396 5157 6185 1540
Size: 275 186 2395 761 1028 2301
Fragments arranged by size:
2395 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2867 3111 4166 5007 5092 6163 2867
Size: 244 1055 841 85 1071 3650
Fragments arranged by size:
3650 1071 1055 841 244 85
BsmI GAATG_Cn'
Cuts at: 2758 4457 4550 2758
Size: 1699 93 5154
Fragments arranged by size:
5154 1699 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3437 3437
Size: 6946
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2822 2849 2867 2900 3099 3111 3811
Size: 399 1032 27 18 33 199 12 700
Cuts at: 3811 3959 4141 4166 4265 5007 5092 6163 1391
Size: 148 182 25 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 1032 742 700 399 199 182
148 99 85 33 27 25 18 12
BspEI T'CCGG_A
Cuts at: 2921 6609 2921
Size: 3688 3258
BspHI T'CATG_A
Cuts at: 4835 6565 4835
Size: 1730 5216
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2058 2155 3738 3982 4193 4301 844
Size: 1214 97 1583 244 211 108 3489
Fragments arranged by size:
3489 1583 1214 244 211 108 97
BsrBI CCG'CTC
Cuts at: 1394 1823 4833 1394
Size: 429 3010 3507
Fragments arranged by size:
3507 3010 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2606 3690 5441 5615 65
Size: 1183 1358 1084 1751 174 1396
Fragments arranged by size:
1751 1396 1358 1183 1084 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2988 5590 5777 1700
Size: 110 1178 2602 187 2869
Fragments arranged by size:
2869 2602 1178 187 110
BsrGI T'GTAC_A
Cuts at: 96 3704 3861 96
Size: 3608 157 3181
Fragments arranged by size:
3608 3181 157
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6946
BssSI C'ACGA_G
Cuts at: 2775 5006 6018 2775
Size: 2231 1012 3703
Fragments arranged by size:
3703 2231 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2077 2161 794
Size: 1283 84 5579
Fragments arranged by size:
5579 1283 84
BstBI TT'CG_AA
Cuts at: 5750 5750
Size: 6946
BstEII G'GTnAC_C
Cuts at: 1184 4147 1184
Size: 2963 3983
BstXI CCAn_nnnn'nTGG
Cuts at: 3911 4231 3911
Size: 320 6626
BstYI r'GATC_y
Cuts at: 1972 3679 4276 4643 5028 5045 6497 6583 6595
Size: 1707 597 367 385 17 1452 86 12
Cuts at: 6595 6941 1972
Size: 346 1977
Fragments arranged by size:
1977 1707 1452 597 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3159 3159
Size: 6946
BtgI C'CryG_G
Cuts at: 513 1065 2004 2016 2059 2352 2733 3010 3951
Size: 552 939 12 43 293 381 277 941
Cuts at: 3951 4152 4170 4200 513
Size: 201 18 30 3259
Fragments arranged by size:
3259 941 939 552 381 293 277 201
43 30 18 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3809 4107 487
Size: 683 56 2583 298 3326
Fragments arranged by size:
3326 2583 683 298 56
BtsI GCAGTG_nn'
Cuts at: 2075 3241 3418 4164 4462 5256 5276 2075
Size: 1166 177 746 298 794 20 3745
Fragments arranged by size:
3745 1166 794 746 298 177 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6946
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4069 4104 529
Size: 35 3505 35 3371
Fragments arranged by size:
3505 3371 35 35
DraI TTT'AAA
Cuts at: 4250 4250
Size: 6946
DraIII CAC_nnn'GTG
Cuts at: 2777 2777
Size: 6946
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2036 5953 809
Size: 507 720 3917 1802
Fragments arranged by size:
3917 1802 720 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2868 2958 4129
Size: 54 1425 50 275 18 1038 90 1171
Cuts at: 4129 4393 5282 6911 8
Size: 264 889 1629 43
Fragments arranged by size:
1629 1425 1171 1038 889 275 264 90
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4393 1537
Size: 275 2581 4090
Fragments arranged by size:
4090 2581 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2060 2225 2649 2655 2810 2933 4022 4876
Size: 707 165 424 6 155 123 1089 854
Cuts at: 4876 1353
Size: 3423
Fragments arranged by size:
3423 1089 854 707 424 165 155 123
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2120 3164 5533 5907 6053
Size: 1064 138 435 96 1044 2369 374 146
Cuts at: 6053 387
Size: 1280
Fragments arranged by size:
2369 1280 1064 1044 435 374 146 138
96
EcoICRI GAG'CTC
Cuts at: 2865 3109 2865
Size: 244 6702
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2206 2345 2651 3650 3823 4127 4180
Size: 393 574 139 306 999 173 304 53
Cuts at: 4180 5009 5606 6393 6661 1239
Size: 829 597 787 268 1524
Fragments arranged by size:
1524 999 829 787 597 574 393 306
304 268 173 139 53
EcoO109I rG'GnC_Cy
Cuts at: 2845 2846 2911 3155 3525 3807 3956 4182 2845
Size: 1 65 244 370 282 149 226 5609
Fragments arranged by size:
5609 370 282 244 226 149 65 1
EcoRI G'AATT_C
Cuts at: 4255 4633 4255
Size: 378 6568
FspI TGC'GCA
Cuts at: 5452 5452
Size: 6946
HaeII r_GCGC'y
Cuts at: 1678 3001 6093 1678
Size: 1323 3092 2531
Fragments arranged by size:
3092 2531 1323
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2238 2270 2903 2935 5587
Size: 32 135 32 666 32 633 32 2652
Cuts at: 5587 5619 5661 5693 1373
Size: 32 42 32 2626
Fragments arranged by size:
2652 2626 666 633 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4290 4536 669
Size: 988 281 2352 246 3079
Fragments arranged by size:
3079 2352 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2597 748
Size: 1849 5097
HpaI GTT'AAC
Cuts at: 4536 4536
Size: 6946
KasI G'GCGC_C
Cuts at: 2997 2997
Size: 6946
KpnI G_GTAC'C
Cuts at: 4271 4271
Size: 6946
MfeI C'AATT_G
Cuts at: 4545 4545
Size: 6946
MluI A'CGCG_T
Cuts at: 6822 6822
Size: 6946
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2698 2724 6060 6244 1305
Size: 1393 26 3336 184 2007
Fragments arranged by size:
3336 2007 1393 184 26
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2960 4131 10
Size: 54 1425 343 1128 1171 2825
Fragments arranged by size:
2825 1425 1171 1128 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3302 3909 4169 4904 5263 5422 518
Size: 648 2136 607 260 735 359 159 2042
Fragments arranged by size:
2136 2042 735 648 607 359 260 159
MspA1I CmG'CkG
Cuts at: 1606 2061 2086 2149 2952 2995 3361 3749 3981
Size: 455 25 63 803 43 366 388 232
Cuts at: 3981 4362 5039 6187 6432 1606
Size: 381 677 1148 245 2120
Fragments arranged by size:
2120 1148 803 677 455 388 381 366
245 232 63 43 25
NarI GG'CG_CC
Cuts at: 2998 2998
Size: 6946
NcoI C'CATG_G
Cuts at: 513 1065 2004 2733 3010 4170 4200 513
Size: 552 939 729 277 1160 30 3259
Fragments arranged by size:
3259 1160 939 729 552 277 30
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6946
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6946
NotI GC'GGCC_GC
Cuts at: 4393 4393
Size: 6946
NspI r_CATG'y
Cuts at: 1132 1229 2515 3490 3778 4048 4056 4304 5849
Size: 97 1286 975 288 270 8 248 1545
Cuts at: 5849 1132
Size: 2229
Fragments arranged by size:
2229 1545 1286 975 288 270 248 97
8
PasI CC'CwG_GG
Cuts at: 1348 4005 1348
Size: 2657 4289
PciI A'CATG_T
Cuts at: 4052 5845 4052
Size: 1793 5153
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6946
PfoI T'CCnGG_A
Cuts at: 2040 2040
Size: 6946
PmeI GTTT'AAAC
Cuts at: 4250 4250
Size: 6946
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2105 2137 5015 5047 6528 6560 2105
Size: 32 2878 32 1481 32 2491
Fragments arranged by size:
2878 2491 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2911 4182 2911
Size: 1271 5675
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6946
PsiI TTA'TAA
Cuts at: 4516 4516
Size: 6946
PspOMI G'GGCC_C
Cuts at: 2845 3807 3955 2845
Size: 962 148 5836
Fragments arranged by size:
5836 962 148
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3845 3877 3845
Size: 32 6914
PstI C_TGCA'G
Cuts at: 830 2154 3006 3248 4298 830
Size: 1324 852 242 1050 3478
Fragments arranged by size:
3478 1324 1050 852 242
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6946
PvuII CAG'CTG
Cuts at: 2086 2149 3749 2086
Size: 63 1600 5283
Fragments arranged by size:
5283 1600 63
SacI G_AGCT'C
Cuts at: 2867 3111 2867
Size: 244 6702
SacII CC_GC'GG
Cuts at: 2062 2062
Size: 6946
SalI G'TCGA_C
Cuts at: 1936 4288 1936
Size: 2352 4594
SapI GCTCTTCn'nnn_
Cuts at: 2060 2225 2810 2933 2060
Size: 165 585 123 6073
Fragments arranged by size:
6073 585 165 123
SbfI CC_TGCA'GG
Cuts at: 3006 4298 3006
Size: 1292 5654
ScaI AGT'ACT
Cuts at: 5194 6906 5194
Size: 1712 5234
SexAI A'CCwGG_T
Cuts at: 2562 2562
Size: 6946
SfcI C'TryA_G
Cuts at: 826 1045 2150 3002 3244 4294 5429 6110 6301
Size: 219 1105 852 242 1050 1135 681 191
Cuts at: 6301 826
Size: 1471
Fragments arranged by size:
1471 1135 1105 1050 852 681 242 219
191
SfoI GGC'GCC
Cuts at: 2999 2999
Size: 6946
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6946
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6946
SmaI CCC'GGG
Cuts at: 1872 4273 1872
Size: 2401 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2824 3917 4117 5050 5951 6213
Size: 197 926 881 1093 200 933 901 262
Cuts at: 6213 820
Size: 1553
Fragments arranged by size:
1553 1093 933 926 901 881 262 200
197
SnaBI TAC'GTA
Cuts at: 493 4737 493
Size: 4244 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6946
SphI G_CATG'C
Cuts at: 1132 2515 4304 1132
Size: 1383 1789 3774
Fragments arranged by size:
3774 1789 1383
SspI AAT'ATT
Cuts at: 5 52 4870 5
Size: 47 4818 2081
Fragments arranged by size:
4818 2081 47
StuI AGG'CCT
Cuts at: 1517 2666 1517
Size: 1149 5797
StyI C'CwwG_G
Cuts at: 513 1065 2004 2667 2733 2901 2928 3010 4123
Size: 552 939 663 66 168 27 82 1113
Cuts at: 4123 4170 4200 4385 513
Size: 47 30 185 3074
Fragments arranged by size:
3074 1113 939 663 552 185 168 82
66 47 30 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4076 4973 4990 5143 5328 5773 325
Size: 1255 2496 897 17 153 185 445 1498
Fragments arranged by size:
2496 1498 1255 897 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3704 3861 3930
Size: 274 80 33 51 1443 1727 157 69
Cuts at: 3930 5192 6904 96
Size: 1262 1712 138
Fragments arranged by size:
1727 1712 1443 1262 274 157 138 80
69 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3215 3247 6906 6938 3215
Size: 32 3659 32 3223
Fragments arranged by size:
3659 3223 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6946
XbaI T'CTAG_A
Cuts at: 4282 4282
Size: 6946
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6946
XmaI C'CCGG_G
Cuts at: 1870 4271 1870
Size: 2401 4545
XmnI GAAnn'nnTTC
Cuts at: 2120 5075 2120
Size: 2955 3991
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2919 4754 276
Size: 53 83 186 936 1385 1835 2468
Fragments arranged by size:
2468 1835 1385 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NarI NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SacI SacII SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AscI BaeI BfrBI BsiWI BstZ17I EcoNI EcoRV
FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI
PmlI PspXI RsrII SanDI SfiI SrfI SwaI XcmI