Restriction map for
pFN21AE0026
(Circular) (Six-base) MAPSORT of: pFN21AE0026.seq Check: 9445 from: 1 to: 6160
pFN21AE0026 6160 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3970 278
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3141 3159 3503 1937
Size: 1204 18 344 4594
Fragments arranged by size:
4594 1204 344 18
Acc65I G'GTAC_C
Cuts at: 3481 3481
Size: 6160
AclI AA'CG_TT
Cuts at: 1563 4287 4660 1563
Size: 2724 373 3063
Fragments arranged by size:
3063 2724 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2303 2339 2480 2918 4223 5607 5875 1632
Size: 671 36 141 438 1305 1384 268 1917
Fragments arranged by size:
1917 1384 1305 671 438 268 141 36
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5963
AflIII A'CryG_T
Cuts at: 1203 2974 5059 6036 1203
Size: 1771 2085 977 1327
Fragments arranged by size:
2085 1771 1327 977
AgeI A'CCGG_T
Cuts at: 4991 4991
Size: 6160
AhdI GACnn_n'nnGTC
Cuts at: 4889 4889
Size: 6160
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6160
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6160
AlwNI CAG_nnn'CTG
Cuts at: 2109 2683 2725 2936 3004 3903 5475 2109
Size: 574 42 211 68 899 1572 2794
Fragments arranged by size:
2794 1572 899 574 211 68 42
ApaLI G'TGCA_C
Cuts at: 2978 4217 5373 2978
Size: 1239 1156 3765
Fragments arranged by size:
3765 1239 1156
ApoI r'AATT_y
Cuts at: 1493 3469 3695 3847 5956 1493
Size: 1976 226 152 2109 1697
Fragments arranged by size:
2109 1976 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4714 160
Size: 872 3682 1606
Fragments arranged by size:
3682 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
AvaI C'yCGr_G
Cuts at: 1870 1943 3485 1870
Size: 73 1542 4545
Fragments arranged by size:
4545 1542 73
BamHI G'GATC_C
Cuts at: 2008 2926 3490 3857 2008
Size: 918 564 367 4311
Fragments arranged by size:
4311 918 564 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3224 3372 3481 4936 618
Size: 325 217 627 1437 148 109 1455 1842
Fragments arranged by size:
1842 1455 1437 627 325 217 148 109
BanII G_rGCy'C
Cuts at: 1391 2082 2191 2706 3479 1391
Size: 691 109 515 773 4072
Fragments arranged by size:
4072 773 691 515 109
BbeI G_GCGC'C
Cuts at: 3376 3376
Size: 6160
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2867 2906 928
Size: 971 968 39 4182
Fragments arranged by size:
4182 971 968 39
BbvCI CC'TCA_GC
Cuts at: 3197 3197
Size: 6160
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4351 4385 1903
Size: 34 2414 34 3678
Fragments arranged by size:
3678 2414 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4052 5262 4052
Size: 1210 4950
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6160
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2221 4771 136
Size: 107 122 71 631 605 549 2550 1525
Fragments arranged by size:
2550 1525 631 605 549 122 107 71
BglII A'GATC_T
Cuts at: 6155 6155
Size: 6160
BlpI GC'TnA_GC
Cuts at: 3577 3577
Size: 6160
Bme1580I G_kGCm'C
Cuts at: 1790 2982 4221 5377 1790
Size: 1192 1239 1156 2573
Fragments arranged by size:
2573 1239 1192 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6160
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2170 2888 3091
Size: 416 53 83 399 24 753 718 203
Cuts at: 3091 3891 4849 442
Size: 800 958 1753
Fragments arranged by size:
1753 958 800 753 718 416 399 203
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6160
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2705 2873 4820 1239
Size: 1466 168 1947 2579
Fragments arranged by size:
2579 1947 1466 168
Bpu10I CC'TnA_GC
Cuts at: 2258 3197 2258
Size: 939 5221
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4285 5150 5448 4285
Size: 865 298 4997
Fragments arranged by size:
4997 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2663 4823 882
Size: 617 1164 2160 2219
Fragments arranged by size:
2219 2160 1164 617
BsaAI yAC'GTr
Cuts at: 493 1204 3951 493
Size: 711 2747 2702
Fragments arranged by size:
2747 2702 711
BsaBI GATnn'nnATC
Cuts at: 6154 6154
Size: 6160
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3373 3967 4349 4984
Size: 53 83 186 936 1840 594 382 635
Cuts at: 4984 275
Size: 1451
Fragments arranged by size:
1840 1451 936 635 594 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3144 4593 4991 5265 5412 5823 3144
Size: 1449 398 274 147 411 3481
Fragments arranged by size:
3481 1449 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2414 2444 3317 3347 1178
Size: 30 165 30 1011 30 873 30 3991
Fragments arranged by size:
3991 1011 873 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3055 3252 3417 1186
Size: 1869 197 165 3929
Fragments arranged by size:
3929 1869 197 165
BseYI C'CCAG_C
Cuts at: 2617 5363 5859 2617
Size: 2746 496 2918
Fragments arranged by size:
2918 2746 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5587
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3610 4371 5399 1540
Size: 275 186 1609 761 1028 2301
Fragments arranged by size:
2301 1609 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2191 2706 2982 4221 4306 5377 2191
Size: 515 276 1239 85 1071 2974
Fragments arranged by size:
2974 1239 1071 515 276 85
BsmI GAATG_Cn'
Cuts at: 3671 3764 3671
Size: 93 6067
BsmBI CGTCTCn'nnnn_
Cuts at: 2954 2954
Size: 6160
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2191 2706 2982 3479 4221 4306
Size: 399 292 109 515 276 497 742 85
Cuts at: 4306 5377 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 515 497 399 292 276
109 85
BspEI T'CCGG_A
Cuts at: 5823 5823
Size: 6160
BspHI T'CATG_A
Cuts at: 2440 4049 5779 2440
Size: 1609 1730 2821
Fragments arranged by size:
2821 1730 1609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2217 3515 844
Size: 1373 1298 3489
Fragments arranged by size:
3489 1373 1298
BsrBI CCG'CTC
Cuts at: 1394 1823 4047 1394
Size: 429 2224 3507
Fragments arranged by size:
3507 2224 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4655 4829 65
Size: 1183 3407 174 1396
Fragments arranged by size:
3407 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3072 3253 4804 4991 1700
Size: 110 1262 181 1551 187 2869
Fragments arranged by size:
2869 1551 1262 187 181 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6160
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6160
BssSI C'ACGA_G
Cuts at: 4220 5232 4220
Size: 1012 5148
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6160
BstBI TT'CG_AA
Cuts at: 4964 4964
Size: 6160
BstEII G'GTnAC_C
Cuts at: 1184 2288 1184
Size: 1104 5056
BstXI CCAn_nnnn'nTGG
Cuts at: 2020 2020
Size: 6160
BstYI r'GATC_y
Cuts at: 1972 2008 2139 2892 2926 3490 3857 4242 4259
Size: 36 131 753 34 564 367 385 17
Cuts at: 4259 5711 5797 5809 6155 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 753 564 385 367 346 131
86 36 34 17 12
Bsu36I CC'TnA_GG
Cuts at: 2072 3067 2072
Size: 995 5165
BtgI C'CryG_G
Cuts at: 513 1065 2004 2305 513
Size: 552 939 301 4368
Fragments arranged by size:
4368 939 552 301
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5421
Fragments arranged by size:
5421 683 56
BtsI GCAGTG_nn'
Cuts at: 3420 3676 4470 4490 3420
Size: 256 794 20 5090
Fragments arranged by size:
5090 794 256 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6125
DraI TTT'AAA
Cuts at: 3464 3464
Size: 6160
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5167 809
Size: 507 3851 1802
Fragments arranged by size:
3851 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2098 2229 2643
Size: 54 1425 50 275 18 268 131 414
Cuts at: 2643 3432 3607 4496 6125 8
Size: 789 175 889 1629 43
Fragments arranged by size:
1629 1425 889 789 414 275 268 175
131 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3607 1537
Size: 275 1795 4090
Fragments arranged by size:
4090 1795 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2469 4090 1353
Size: 1116 1621 3423
Fragments arranged by size:
3423 1621 1116
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4747 5121 5267 387
Size: 1064 138 3158 374 146 1280
Fragments arranged by size:
3158 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2189 2704 2189
Size: 515 5645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2303 2339 2480 2705 2873 2918 4223
Size: 393 671 36 141 225 168 45 1305
Cuts at: 4223 4820 5607 5875 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1305 787 671 597 393 268 225
168 141 45 36
EcoNI CCTnn'n_nnAGG
Cuts at: 2071 2071
Size: 6160
EcoO109I rG'GnC_Cy
Cuts at: 2446 2722 3115 2446
Size: 276 393 5491
Fragments arranged by size:
5491 393 276
EcoRI G'AATT_C
Cuts at: 3469 3847 3469
Size: 378 5782
FspI TGC'GCA
Cuts at: 4666 4666
Size: 6160
HaeII r_GCGC'y
Cuts at: 1678 2305 3376 5307 1678
Size: 627 1071 1931 2531
Fragments arranged by size:
2531 1931 1071 627
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2178 2210 3317 3349 4801
Size: 32 135 32 606 32 1107 32 1452
Cuts at: 4801 4833 4875 4907 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1452 1107 606 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3504 3750 669
Size: 988 281 1566 246 3079
Fragments arranged by size:
3079 1566 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2486 748
Size: 1738 4422
HpaI GTT'AAC
Cuts at: 3750 3750
Size: 6160
KasI G'GCGC_C
Cuts at: 3372 3372
Size: 6160
KpnI G_GTAC'C
Cuts at: 3485 3485
Size: 6160
MfeI C'AATT_G
Cuts at: 3759 3759
Size: 6160
MluI A'CGCG_T
Cuts at: 6036 6036
Size: 6160
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5274 5458 1305
Size: 3969 184 2007
Fragments arranged by size:
3969 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2100 2231 2645 3434 10
Size: 54 1425 343 268 131 414 789 2736
Fragments arranged by size:
2736 1425 789 414 343 268 131 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2060 4118 4477 4636 518
Size: 648 894 2058 359 159 2042
Fragments arranged by size:
2058 2042 894 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2118 2227 2617 2656 2666 2775 2912 3576
Size: 512 109 390 39 10 109 137 664
Cuts at: 3576 4253 5401 5646 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 664 512 390 245 137
109 109 39 10
NaeI GCC'GGC
Cuts at: 3074 3074
Size: 6160
NarI GG'CG_CC
Cuts at: 3373 3373
Size: 6160
NcoI C'CATG_G
Cuts at: 513 1065 2004 2305 513
Size: 552 939 301 4368
Fragments arranged by size:
4368 939 552 301
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6160
NgoMIV G'CCGG_C
Cuts at: 3072 3072
Size: 6160
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6160
NotI GC'GGCC_GC
Cuts at: 3607 3607
Size: 6160
NspI r_CATG'y
Cuts at: 1132 1229 3518 5063 1132
Size: 97 2289 1545 2229
Fragments arranged by size:
2289 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3368 1348
Size: 2020 4140
PciI A'CATG_T
Cuts at: 5059 5059
Size: 6160
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6160
PfoI T'CCnGG_A
Cuts at: 2269 2269
Size: 6160
PmeI GTTT'AAAC
Cuts at: 3464 3464
Size: 6160
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4229 4261 5742 5774 4229
Size: 32 1481 32 4615
Fragments arranged by size:
4615 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2722 2722
Size: 6160
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6160
PsiI TTA'TAA
Cuts at: 3730 3730
Size: 6160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2150 2182 2150
Size: 32 6128
PstI C_TGCA'G
Cuts at: 830 2366 3512 830
Size: 1536 1146 3478
Fragments arranged by size:
3478 1536 1146
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6160
PvuII CAG'CTG
Cuts at: 2118 2227 2617 2912 2118
Size: 109 390 295 5366
Fragments arranged by size:
5366 390 295 109
SacI G_AGCT'C
Cuts at: 2191 2706 2191
Size: 515 5645
SalI G'TCGA_C
Cuts at: 1936 3502 1936
Size: 1566 4594
SbfI CC_TGCA'GG
Cuts at: 3512 3512
Size: 6160
ScaI AGT'ACT
Cuts at: 4408 6120 4408
Size: 1712 4448
SfcI C'TryA_G
Cuts at: 826 1045 2362 2651 3400 3508 4643 5324 5515
Size: 219 1317 289 749 108 1135 681 191
Cuts at: 5515 826
Size: 1471
Fragments arranged by size:
1471 1317 1135 749 681 289 219 191
108
SfoI GGC'GCC
Cuts at: 3374 3374
Size: 6160
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6160
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6160
SmaI CCC'GGG
Cuts at: 1872 3487 1872
Size: 1615 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4264 5165 5427 820
Size: 197 926 2321 901 262 1553
Fragments arranged by size:
2321 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3951 493
Size: 3458 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6160
SphI G_CATG'C
Cuts at: 1132 3518 1132
Size: 2386 3774
SspI AAT'ATT
Cuts at: 5 52 4084 5
Size: 47 4032 2081
Fragments arranged by size:
4032 2081 47
StuI AGG'CCT
Cuts at: 1517 2360 2480 2767 1517
Size: 843 120 287 4910
Fragments arranged by size:
4910 843 287 120
StyI C'CwwG_G
Cuts at: 513 1065 2004 2019 2201 2305 3599 513
Size: 552 939 15 182 104 1294 3074
Fragments arranged by size:
3074 1294 939 552 182 104 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3175 4187 4204 4357 4542 4987 325
Size: 1255 1595 1012 17 153 185 445 1498
Fragments arranged by size:
1595 1498 1255 1012 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2424 2875 4406
Size: 274 80 33 51 1443 447 451 1531
Cuts at: 4406 6118 96
Size: 1712 138
Fragments arranged by size:
1712 1531 1443 451 447 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2414 2446 3171 3203 3267 3299 6120 6152 2414
Size: 32 725 32 64 32 2821 32 2422
Fragments arranged by size:
2821 2422 725 64 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6160
XbaI T'CTAG_A
Cuts at: 3496 3496
Size: 6160
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2020 2680 3442 2020
Size: 660 762 4738
Fragments arranged by size:
4738 762 660
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6160
XmaI C'CCGG_G
Cuts at: 1870 3485 1870
Size: 1615 4545
XmnI GAAnn'nnTTC
Cuts at: 2324 4289 2324
Size: 1965 4195
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3968 276
Size: 53 83 186 936 2434 2468
Fragments arranged by size:
2468 2434 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BplI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspOMI PspXI RsrII SacII
SanDI SapI SexAI SfiI SrfI SwaI