Restriction map for
pFN21AE0019
(Circular) (Six-base) MAPSORT of: pFN21AE0019.seq Check: 5505 from: 1 to: 6481
pFN21AE0019 6481 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4291 278
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3824 1937
Size: 1887 4594
Acc65I G'GTAC_C
Cuts at: 3802 3802
Size: 6481
AclI AA'CG_TT
Cuts at: 1563 4608 4981 1563
Size: 3045 373 3063
Fragments arranged by size:
3063 3045 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2940 4544 5928 6196 1632
Size: 1308 1604 1384 268 1917
Fragments arranged by size:
1917 1604 1384 1308 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6284
AflIII A'CryG_T
Cuts at: 1203 5380 6357 1203
Size: 4177 977 1327
Fragments arranged by size:
4177 1327 977
AgeI A'CCGG_T
Cuts at: 5312 5312
Size: 6481
AhdI GACnn_n'nnGTC
Cuts at: 5210 5210
Size: 6481
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6481
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3044 1184
Size: 1860 4621
AlwNI CAG_nnn'CTG
Cuts at: 4224 5796 4224
Size: 1572 4909
ApaI G_GGCC'C
Cuts at: 2281 2281
Size: 6481
ApaLI G'TGCA_C
Cuts at: 3633 4538 5694 3633
Size: 905 1156 4420
Fragments arranged by size:
4420 1156 905
ApoI r'AATT_y
Cuts at: 1493 2930 3790 4016 4168 6277 1493
Size: 1437 860 226 152 2109 1697
Fragments arranged by size:
2109 1697 1437 860 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3108 5035 160
Size: 872 2076 1927 1606
Fragments arranged by size:
2076 1927 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
AvaI C'yCGr_G
Cuts at: 1870 1943 3806 1870
Size: 73 1863 4545
Fragments arranged by size:
4545 1863 73
BamHI G'GATC_C
Cuts at: 3811 4178 3811
Size: 367 6114
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3802 5257 618
Size: 325 217 627 2015 1455 1842
Fragments arranged by size:
2015 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2281 3369 3800 1391
Size: 890 1088 431 4072
Fragments arranged by size:
4072 1088 890 431
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3267 928
Size: 971 1368 4142
Fragments arranged by size:
4142 1368 971
BbvCI CC'TCA_GC
Cuts at: 3013 3013
Size: 6481
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4672 4706 1903
Size: 34 2735 34 3678
Fragments arranged by size:
3678 2735 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4373 5583 4373
Size: 1210 5271
BclI T'GATC_A
Cuts at: 1548 3702 1548
Size: 2154 4327
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5092 136
Size: 107 122 71 631 605 3420 1525
Fragments arranged by size:
3420 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6476 6476
Size: 6481
BlpI GC'TnA_GC
Cuts at: 3898 3898
Size: 6481
Bme1580I G_kGCm'C
Cuts at: 1790 2281 3637 4542 5698 1790
Size: 491 1356 905 1156 2573
Fragments arranged by size:
2573 1356 1156 905 491
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6481
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2233 3736 4212
Size: 416 53 83 399 24 816 1503 476
Cuts at: 4212 5170 442
Size: 958 1753
Fragments arranged by size:
1753 1503 958 816 476 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6481
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3076 3076
Size: 6481
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2576 3588 5141 1239
Size: 1337 1012 1553 2579
Fragments arranged by size:
2579 1553 1337 1012
Bpu10I CC'TnA_GC
Cuts at: 3013 3013
Size: 6481
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2141 4606 5471 5769 2141
Size: 2465 865 298 2853
Fragments arranged by size:
2853 2465 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2392 2900 5144 882
Size: 617 893 508 2244 2219
Fragments arranged by size:
2244 2219 893 617 508
BsaAI yAC'GTr
Cuts at: 493 1204 4272 493
Size: 711 3068 2702
Fragments arranged by size:
3068 2702 711
BsaBI GATnn'nnATC
Cuts at: 6475 6475
Size: 6481
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4288 4670 5305 275
Size: 53 83 186 936 2755 382 635 1451
Fragments arranged by size:
2755 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4914 5312 5586 5733 6144 4914
Size: 398 274 147 411 5251
Fragments arranged by size:
5251 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6256
Fragments arranged by size:
6256 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2937 2940 1186
Size: 1751 3 4727
Fragments arranged by size:
4727 1751 3
BseYI C'CCAG_C
Cuts at: 2640 5684 6180 2640
Size: 3044 496 2941
Fragments arranged by size:
3044 2941 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3062 3321 3638 1163
Size: 573 1326 259 317 4006
Fragments arranged by size:
4006 1326 573 317 259
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3931 4692 5720 1540
Size: 275 186 1930 761 1028 2301
Fragments arranged by size:
2301 1930 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3057 3637 4542 4627 5698 3057
Size: 580 905 85 1071 3840
Fragments arranged by size:
3840 1071 905 580 85
BsmI GAATG_Cn'
Cuts at: 3992 4085 3992
Size: 93 6388
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2281 3057 3369 3637 3800 4542 4627
Size: 399 491 776 312 268 163 742 85
Cuts at: 4627 5698 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 776 742 491 399 312 268
163 85
BspEI T'CCGG_A
Cuts at: 6144 6144
Size: 6481
BspHI T'CATG_A
Cuts at: 4370 6100 4370
Size: 1730 4751
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3015 3660 3836 844
Size: 2171 645 176 3489
Fragments arranged by size:
3489 2171 645 176
BsrBI CCG'CTC
Cuts at: 1394 1823 4368 1394
Size: 429 2545 3507
Fragments arranged by size:
3507 2545 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4976 5150 65
Size: 1183 3728 174 1396
Fragments arranged by size:
3728 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5125 5312 1700
Size: 110 3315 187 2869
Fragments arranged by size:
3315 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2341 96
Size: 2245 4236
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6481
BssSI C'ACGA_G
Cuts at: 4541 5553 4541
Size: 1012 5469
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3411 794
Size: 2617 3864
BstBI TT'CG_AA
Cuts at: 5285 5285
Size: 6481
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6481
BstYI r'GATC_y
Cuts at: 1972 2698 3811 4178 4563 4580 6032 6118 6130
Size: 726 1113 367 385 17 1452 86 12
Cuts at: 6130 6476 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1113 726 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3673 513
Size: 552 2608 3321
Fragments arranged by size:
3321 2608 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5742
Fragments arranged by size:
5742 683 56
BtsI GCAGTG_nn'
Cuts at: 3309 3997 4791 4811 3309
Size: 688 794 20 4979
Fragments arranged by size:
4979 794 688 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6481
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6446
DraI TTT'AAA
Cuts at: 2502 3785 2502
Size: 1283 5198
DraIII CAC_nnn'GTG
Cuts at: 3632 3632
Size: 6481
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5488 809
Size: 507 4172 1802
Fragments arranged by size:
4172 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2326 3384 3928
Size: 54 1425 50 275 18 496 1058 544
Cuts at: 3928 4817 6446 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1058 889 544 496 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3928 1537
Size: 275 2116 4090
Fragments arranged by size:
4090 2116 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3045 4411 1353
Size: 1692 1366 3423
Fragments arranged by size:
3423 1692 1366
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5068 5442 5588 387
Size: 1064 138 3479 374 146 1280
Fragments arranged by size:
3479 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2576 2940 3588 4544 5141 5928 6196
Size: 393 944 364 648 956 597 787 268
Cuts at: 6196 1239
Size: 1524
Fragments arranged by size:
1524 956 944 787 648 597 393 364
268
EcoO109I rG'GnC_Cy
Cuts at: 2277 2277
Size: 6481
EcoRI G'AATT_C
Cuts at: 3790 4168 3790
Size: 378 6103
FspI TGC'GCA
Cuts at: 4987 4987
Size: 6481
HaeII r_GCGC'y
Cuts at: 1678 5628 1678
Size: 3950 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2353 2385 2996 3028 5122
Size: 32 135 32 781 32 611 32 2094
Cuts at: 5122 5154 5196 5228 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2094 781 611 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3825 4071 669
Size: 988 281 1887 246 3079
Fragments arranged by size:
3079 1887 988 281 246
HindIII A'AGCT_T
Cuts at: 748 3514 748
Size: 2766 3715
HpaI GTT'AAC
Cuts at: 4071 4071
Size: 6481
KpnI G_GTAC'C
Cuts at: 3806 3806
Size: 6481
MfeI C'AATT_G
Cuts at: 4080 4080
Size: 6481
MluI A'CGCG_T
Cuts at: 6357 6357
Size: 6481
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2051 2230 3404 3552 5595 5779 1305
Size: 746 179 1174 148 2043 184 2007
Fragments arranged by size:
2043 2007 1174 746 184 179 148
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2328 3386 10
Size: 54 1425 343 496 1058 3105
Fragments arranged by size:
3105 1425 1058 496 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2190 3695 4439 4798 4957 518
Size: 648 1024 1505 744 359 159 2042
Fragments arranged by size:
2042 1505 1024 744 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2339 3017 3897 4574 5722 5967 1606
Size: 733 678 880 677 1148 245 2120
Fragments arranged by size:
2120 1148 880 733 678 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5929
NdeI CA'TA_TG
Cuts at: 387 3228 387
Size: 2841 3640
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6481
NotI GC'GGCC_GC
Cuts at: 3928 3928
Size: 6481
NspI r_CATG'y
Cuts at: 1132 1229 3031 3599 3839 5384 1132
Size: 97 1802 568 240 1545 2229
Fragments arranged by size:
2229 1802 1545 568 240 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6481
PciI A'CATG_T
Cuts at: 5380 5380
Size: 6481
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6481
PmeI GTTT'AAAC
Cuts at: 3785 3785
Size: 6481
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4550 4582 6063 6095 4550
Size: 32 1481 32 4936
Fragments arranged by size:
4936 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6481
PsiI TTA'TAA
Cuts at: 4051 4051
Size: 6481
PspOMI G'GGCC_C
Cuts at: 2277 2277
Size: 6481
PstI C_TGCA'G
Cuts at: 830 3560 3833 830
Size: 2730 273 3478
Fragments arranged by size:
3478 2730 273
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6481
PvuII CAG'CTG
Cuts at: 2339 3017 2339
Size: 678 5803
SalI G'TCGA_C
Cuts at: 1936 3823 1936
Size: 1887 4594
SapI GCTCTTCn'nnn_
Cuts at: 3045 3045
Size: 6481
SbfI CC_TGCA'GG
Cuts at: 3833 3833
Size: 6481
ScaI AGT'ACT
Cuts at: 4729 6441 4729
Size: 1712 4769
SfcI C'TryA_G
Cuts at: 826 1045 3185 3556 3829 4964 5645 5836 826
Size: 219 2140 371 273 1135 681 191 1471
Fragments arranged by size:
2140 1471 1135 681 371 273 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6481
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6481
SmaI CCC'GGG
Cuts at: 1872 3808 1872
Size: 1936 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2120 4585 5486 5748 820
Size: 197 926 177 2465 901 262 1553
Fragments arranged by size:
2465 1553 926 901 262 197 177
SnaBI TAC'GTA
Cuts at: 493 4272 493
Size: 3779 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6481
SphI G_CATG'C
Cuts at: 1132 3031 3839 1132
Size: 1899 808 3774
Fragments arranged by size:
3774 1899 808
SspI AAT'ATT
Cuts at: 5 52 2170 3497 4405 5
Size: 47 2118 1327 908 2081
Fragments arranged by size:
2118 2081 1327 908 47
StuI AGG'CCT
Cuts at: 1517 3038 1517
Size: 1521 4960
StyI C'CwwG_G
Cuts at: 513 1065 3920 513
Size: 552 2855 3074
Fragments arranged by size:
3074 2855 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4508 4525 4678 4863 5308 325
Size: 1255 2928 17 153 185 445 1498
Fragments arranged by size:
2928 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2341 3251 4727
Size: 274 80 33 51 1443 364 910 1476
Cuts at: 4727 6439 96
Size: 1712 138
Fragments arranged by size:
1712 1476 1443 910 364 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6441 6473 6441
Size: 32 6449
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6481
XbaI T'CTAG_A
Cuts at: 2944 3437 3817 2944
Size: 493 380 5608
Fragments arranged by size:
5608 493 380
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2246 2648 3094 2246
Size: 402 446 5633
Fragments arranged by size:
5633 446 402
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6481
XmaI C'CCGG_G
Cuts at: 1870 3806 1870
Size: 1936 4545
XmnI GAAnn'nnTTC
Cuts at: 2202 4610 2202
Size: 2408 4073
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4289 276
Size: 53 83 186 936 2755 2468
Fragments arranged by size:
2755 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI
BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII
SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI