Restriction map for
pFN21AE0017
(Circular) (Six-base) MAPSORT of: pFN21AE0017.seq Check: 9409 from: 1 to: 6355
pFN21AE0017 6355 bp
With 182 enzymes: SgfI *
April 15, 2010 09:25 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4165 278
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3698 1937
Size: 1761 4594
Acc65I G'GTAC_C
Cuts at: 3676 3676
Size: 6355
AclI AA'CG_TT
Cuts at: 1563 2989 4482 4855 1563
Size: 1426 1493 373 3063
Fragments arranged by size:
3063 1493 1426 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2140 2532 3099 4418 5802 6070 1632
Size: 508 392 567 1319 1384 268 1917
Fragments arranged by size:
1917 1384 1319 567 508 392 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6158
AflIII A'CryG_T
Cuts at: 1203 3411 5254 6231 1203
Size: 2208 1843 977 1327
Fragments arranged by size:
2208 1843 1327 977
AgeI A'CCGG_T
Cuts at: 5186 5186
Size: 6355
AhdI GACnn_n'nnGTC
Cuts at: 2341 5084 2341
Size: 2743 3612
AleI CACnn'nnGTG
Cuts at: 1166 3239 1166
Size: 2073 4282
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3241 1184
Size: 2057 4298
AlwNI CAG_nnn'CTG
Cuts at: 2948 3470 4098 5670 2948
Size: 522 628 1572 3633
Fragments arranged by size:
3633 1572 628 522
ApaLI G'TGCA_C
Cuts at: 4412 5568 4412
Size: 1156 5199
ApoI r'AATT_y
Cuts at: 1493 2254 3664 3890 4042 6151 1493
Size: 761 1410 226 152 2109 1697
Fragments arranged by size:
2109 1697 1410 761 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4909 160
Size: 872 3877 1606
Fragments arranged by size:
3877 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
AvaI C'yCGr_G
Cuts at: 1870 1943 3680 1870
Size: 73 1737 4545
Fragments arranged by size:
4545 1737 73
AvrII C'CTAG_G
Cuts at: 2426 2426
Size: 6355
BamHI G'GATC_C
Cuts at: 3685 4052 3685
Size: 367 5988
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3676 5131 618
Size: 325 217 627 1889 1455 1842
Fragments arranged by size:
1889 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2036 3674 1391
Size: 645 1638 4072
Fragments arranged by size:
4072 1638 645
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2043 2178 2361 2619 928
Size: 971 144 135 183 258 4664
Fragments arranged by size:
4664 971 258 183 144 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4546 4580 1903
Size: 34 2609 34 3678
Fragments arranged by size:
3678 2609 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4247 5457 4247
Size: 1210 5145
BclI T'GATC_A
Cuts at: 1548 3151 1548
Size: 1603 4752
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3554 4966 136
Size: 107 122 71 631 605 1882 1412 1525
Fragments arranged by size:
1882 1525 1412 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6350 6350
Size: 6355
BlpI GC'TnA_GC
Cuts at: 3772 3772
Size: 6355
Bme1580I G_kGCm'C
Cuts at: 1790 3619 4416 5572 1790
Size: 1829 797 1156 2573
Fragments arranged by size:
2573 1829 1156 797
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6355
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2030 3281 4086
Size: 416 53 83 399 24 613 1251 805
Cuts at: 4086 5044 442
Size: 958 1753
Fragments arranged by size:
1753 1251 958 805 613 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3069 5015 1239
Size: 1830 1946 2579
Fragments arranged by size:
2579 1946 1830
Bpu10I CC'TnA_GC
Cuts at: 3138 3138
Size: 6355
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2753 2814 4480 5345 5643 2753
Size: 61 1666 865 298 3465
Fragments arranged by size:
3465 1666 865 298 61
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3427 3590 5018 882
Size: 617 1928 163 1428 2219
Fragments arranged by size:
2219 1928 1428 617 163
BsaAI yAC'GTr
Cuts at: 493 1204 4146 493
Size: 711 2942 2702
Fragments arranged by size:
2942 2702 711
BsaBI GATnn'nnATC
Cuts at: 2907 6349 2907
Size: 3442 2913
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2387 4162 4544 5179
Size: 53 83 186 936 854 1775 382 635
Cuts at: 5179 275
Size: 1451
Fragments arranged by size:
1775 1451 936 854 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4788 5186 5460 5607 6018 4788
Size: 398 274 147 411 5125
Fragments arranged by size:
5125 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6130
Fragments arranged by size:
6130 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6355
BseYI C'CCAG_C
Cuts at: 3338 5558 6054 3338
Size: 2220 496 3639
Fragments arranged by size:
3639 2220 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3436 1163
Size: 573 1700 4082
Fragments arranged by size:
4082 1700 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3805 4566 5594 1540
Size: 275 186 1804 761 1028 2301
Fragments arranged by size:
2301 1804 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4416 4501 5572 4416
Size: 85 1071 5199
Fragments arranged by size:
5199 1071 85
BsmI GAATG_Cn'
Cuts at: 2216 2867 2960 3866 3959 2216
Size: 651 93 906 93 4612
Fragments arranged by size:
4612 906 651 93 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 3619 3674 4416 4501 5572 1391
Size: 399 246 1583 55 742 85 1071 2174
Fragments arranged by size:
2174 1583 1071 742 399 246 85 55
BspEI T'CCGG_A
Cuts at: 6018 6018
Size: 6355
BspHI T'CATG_A
Cuts at: 2062 4244 5974 2062
Size: 2182 1730 2443
Fragments arranged by size:
2443 2182 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3710 844
Size: 2866 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4242 1394
Size: 429 2419 3507
Fragments arranged by size:
3507 2419 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2821 3349 3459 4850 5024 65
Size: 1183 1573 528 110 1391 174 1396
Fragments arranged by size:
1573 1396 1391 1183 528 174 110
BsrFI r'CCGG_y
Cuts at: 1700 1810 3132 4999 5186 1700
Size: 110 1322 1867 187 2869
Fragments arranged by size:
2869 1867 1322 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6355
BssSI C'ACGA_G
Cuts at: 4415 5427 4415
Size: 1012 5343
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6355
BstBI TT'CG_AA
Cuts at: 5159 5159
Size: 6355
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6355
BstXI CCAn_nnnn'nTGG
Cuts at: 3047 3047
Size: 6355
BstYI r'GATC_y
Cuts at: 1972 3685 4052 4437 4454 5906 5992 6004 6350
Size: 1713 367 385 17 1452 86 12 346
Cuts at: 6350 1972
Size: 1977
Fragments arranged by size:
1977 1713 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3286 513
Size: 552 939 1282 3582
Fragments arranged by size:
3582 1282 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5616
Fragments arranged by size:
5616 683 56
BtsI GCAGTG_nn'
Cuts at: 2076 2701 3180 3424 3871 4665 4685 2076
Size: 625 479 244 447 794 20 3746
Fragments arranged by size:
3746 794 625 479 447 244 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6355
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6320
DraI TTT'AAA
Cuts at: 3659 3659
Size: 6355
DraIII CAC_nnn'GTG
Cuts at: 3287 3287
Size: 6355
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5362 809
Size: 507 4046 1802
Fragments arranged by size:
4046 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3802 4691 6320
Size: 54 1425 50 275 18 1972 889 1629
Cuts at: 6320 8
Size: 43
Fragments arranged by size:
1972 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3802 1537
Size: 275 1990 4090
Fragments arranged by size:
4090 1990 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2121 3315 3417 4285 1353
Size: 768 1194 102 868 3423
Fragments arranged by size:
3423 1194 868 768 102
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 4942 5316 5462 387
Size: 1064 138 435 2918 374 146 1280
Fragments arranged by size:
2918 1280 1064 435 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2140 2532 3069 3099 4418 5015 5802
Size: 393 508 392 537 30 1319 597 787
Cuts at: 5802 6070 1239
Size: 268 1524
Fragments arranged by size:
1524 1319 787 597 537 508 393 392
268 30
EcoRI G'AATT_C
Cuts at: 3664 4042 3664
Size: 378 5977
FspI TGC'GCA
Cuts at: 4861 4861
Size: 6355
HaeII r_GCGC'y
Cuts at: 1678 5502 1678
Size: 3824 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 2453 2485 4996
Size: 32 135 32 755 32 94 32 2511
Cuts at: 4996 5028 5070 5102 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2511 755 135 94 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3699 3945 669
Size: 988 281 1761 246 3079
Fragments arranged by size:
3079 1761 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2869 3090 748
Size: 2121 221 4013
Fragments arranged by size:
4013 2121 221
HpaI GTT'AAC
Cuts at: 3945 3945
Size: 6355
KpnI G_GTAC'C
Cuts at: 3680 3680
Size: 6355
MfeI C'AATT_G
Cuts at: 3954 3954
Size: 6355
MluI A'CGCG_T
Cuts at: 6231 6231
Size: 6355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5469 5653 1305
Size: 4164 184 2007
Fragments arranged by size:
4164 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4533
Fragments arranged by size:
4533 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 3239 4313 4672 4831 518
Size: 648 934 1139 1074 359 159 2042
Fragments arranged by size:
2042 1139 1074 934 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3771 4448 5596 5841 1606
Size: 2165 677 1148 245 2120
Fragments arranged by size:
2165 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4864
Fragments arranged by size:
4864 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6355
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6355
NotI GC'GGCC_GC
Cuts at: 3802 3802
Size: 6355
NspI r_CATG'y
Cuts at: 1132 1229 2454 2882 3415 3713 5258 1132
Size: 97 1225 428 533 298 1545 2229
Fragments arranged by size:
2229 1545 1225 533 428 298 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6355
PciI A'CATG_T
Cuts at: 3411 5254 3411
Size: 1843 4512
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6355
PmeI GTTT'AAAC
Cuts at: 3659 3659
Size: 6355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2182 2214 2868 2900 4424 4456 5937 5969 2182
Size: 32 654 32 1524 32 1481 32 2568
Fragments arranged by size:
2568 1524 1481 654 32 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6355
PsiI TTA'TAA
Cuts at: 3925 3925
Size: 6355
PstI C_TGCA'G
Cuts at: 830 2725 3707 830
Size: 1895 982 3478
Fragments arranged by size:
3478 1895 982
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6355
SalI G'TCGA_C
Cuts at: 1936 3697 1936
Size: 1761 4594
SbfI CC_TGCA'GG
Cuts at: 3707 3707
Size: 6355
ScaI AGT'ACT
Cuts at: 4603 6315 4603
Size: 1712 4643
SfcI C'TryA_G
Cuts at: 826 1045 2721 3703 4838 5519 5710 826
Size: 219 1676 982 1135 681 191 1471
Fragments arranged by size:
1676 1471 1135 982 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6355
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6355
SmaI CCC'GGG
Cuts at: 1872 3682 1872
Size: 1810 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 2829 4459 5360 5622 820
Size: 197 926 825 61 1630 901 262 1553
Fragments arranged by size:
1630 1553 926 901 825 262 197 61
SnaBI TAC'GTA
Cuts at: 493 4146 493
Size: 3653 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6355
SphI G_CATG'C
Cuts at: 1132 3713 1132
Size: 2581 3774
SspI AAT'ATT
Cuts at: 5 52 4279 5
Size: 47 4227 2081
Fragments arranged by size:
4227 2081 47
StuI AGG'CCT
Cuts at: 1517 2431 2825 1517
Size: 914 394 5047
Fragments arranged by size:
5047 914 394
StyI C'CwwG_G
Cuts at: 513 1065 2004 2426 3794 513
Size: 552 939 422 1368 3074
Fragments arranged by size:
3074 1368 939 552 422
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3086 3497 4382 4399 4552 4737 5182
Size: 1255 1506 411 885 17 153 185 445
Cuts at: 5182 325
Size: 1498
Fragments arranged by size:
1506 1498 1255 885 445 411 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4601 6313 96
Size: 274 80 33 51 1443 2624 1712 138
Fragments arranged by size:
2624 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2680 2712 6315 6347 2680
Size: 32 3603 32 2688
Fragments arranged by size:
3603 2688 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6355
XbaI T'CTAG_A
Cuts at: 2200 3691 2200
Size: 1491 4864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3550 3550
Size: 6355
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6355
XmaI C'CCGG_G
Cuts at: 1870 3680 1870
Size: 1810 4545
XmnI GAAnn'nnTTC
Cuts at: 4484 4484
Size: 6355
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4163 276
Size: 53 83 186 936 2629 2468
Fragments arranged by size:
2629 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI
EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI