Restriction map for
pFN21AE0013
(Circular) (Six-base) MAPSORT of: pFN21AE0013.seq Check: 1395 from: 1 to: 6874
pFN21AE0013 6874 bp
With 182 enzymes: SgfI *
August 16, 2010 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4684 278
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4116 4217 1937
Size: 2179 101 4594
Fragments arranged by size:
4594 2179 101
Acc65I G'GTAC_C
Cuts at: 4195 4195
Size: 6874
AclI AA'CG_TT
Cuts at: 1563 5001 5374 1563
Size: 3438 373 3063
Fragments arranged by size:
3438 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2046 2408 2987 3371 4937 6321 6589 1632
Size: 414 362 579 384 1566 1384 268 1917
Fragments arranged by size:
1917 1566 1384 579 414 384 362 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6677
AflIII A'CryG_T
Cuts at: 1203 2131 5773 6750 1203
Size: 928 3642 977 1327
Fragments arranged by size:
3642 1327 977 928
AgeI A'CCGG_T
Cuts at: 5705 5705
Size: 6874
AhdI GACnn_n'nnGTC
Cuts at: 5603 5603
Size: 6874
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3617 1184
Size: 2433 4441
AlwNI CAG_nnn'CTG
Cuts at: 4617 6189 4617
Size: 1572 5302
ApaLI G'TGCA_C
Cuts at: 4931 6087 4931
Size: 1156 5718
ApoI r'AATT_y
Cuts at: 1493 2234 3143 3158 3181 3677 4183 4409 4561
Size: 741 909 15 23 496 506 226 152
Cuts at: 4561 6670 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 909 741 506 496 226 152
23 15
AseI AT'TA_AT
Cuts at: 160 1032 3054 3336 3650 3662 5428 160
Size: 872 2022 282 314 12 1766 1606
Fragments arranged by size:
2022 1766 1606 872 314 282 12
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
AvaI C'yCGr_G
Cuts at: 1870 1943 4199 1870
Size: 73 2256 4545
Fragments arranged by size:
4545 2256 73
BamHI G'GATC_C
Cuts at: 4204 4571 4204
Size: 367 6507
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2329 3223 4195 5650 618
Size: 325 217 627 542 894 972 1455 1842
Fragments arranged by size:
1842 1455 972 894 627 542 325 217
BanII G_rGCy'C
Cuts at: 1391 2125 4193 1391
Size: 734 2068 4072
Fragments arranged by size:
4072 2068 734
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2052 2396 3252 928
Size: 971 153 344 856 4550
Fragments arranged by size:
4550 971 856 344 153
BbvCI CC'TCA_GC
Cuts at: 3882 3882
Size: 6874
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5065 5099 1903
Size: 34 3128 34 3678
Fragments arranged by size:
3678 3128 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2433 4766 5976 2433
Size: 2333 1210 3331
Fragments arranged by size:
3331 2333 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6874
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5485 136
Size: 107 122 71 631 605 3813 1525
Fragments arranged by size:
3813 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6869 6869
Size: 6874
BlpI GC'TnA_GC
Cuts at: 4291 4291
Size: 6874
Bme1580I G_kGCm'C
Cuts at: 1790 2668 3135 4935 6091 1790
Size: 878 467 1800 1156 2573
Fragments arranged by size:
2573 1800 1156 878 467
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6874
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2671 4605 5563
Size: 416 53 83 399 24 1254 1934 958
Cuts at: 5563 442
Size: 1753
Fragments arranged by size:
1934 1753 1254 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 2442 2579 1056
Size: 1386 137 5351
Fragments arranged by size:
5351 1386 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3714 5534 1239
Size: 2475 1820 2579
Fragments arranged by size:
2579 2475 1820
Bpu10I CC'TnA_GC
Cuts at: 3882 3882
Size: 6874
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3216 3821 4999 5864 6162 3216
Size: 605 1178 865 298 3928
Fragments arranged by size:
3928 1178 865 605 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3623 5537 882
Size: 617 2124 1914 2219
Fragments arranged by size:
2219 2124 1914 617
BsaAI yAC'GTr
Cuts at: 493 1204 3412 4665 493
Size: 711 2208 1253 2702
Fragments arranged by size:
2702 2208 1253 711
BsaBI GATnn'nnATC
Cuts at: 3828 6868 3828
Size: 3040 3834
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4681 5063 5698 275
Size: 53 83 186 936 3148 382 635 1451
Fragments arranged by size:
3148 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2425 5307 5705 5979 6126 6537 2425
Size: 2882 398 274 147 411 2762
Fragments arranged by size:
2882 2762 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6649
Fragments arranged by size:
6649 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3191 1186
Size: 2005 4869
BseYI C'CCAG_C
Cuts at: 6077 6573 6077
Size: 496 6378
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2426 2679 1163
Size: 573 690 253 5358
Fragments arranged by size:
5358 690 573 253
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4324 5085 6113 1540
Size: 275 186 2323 761 1028 2301
Fragments arranged by size:
2323 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2125 4935 5020 6091 2125
Size: 2810 85 1071 2908
Fragments arranged by size:
2908 2810 1071 85
BsmI GAATG_Cn'
Cuts at: 2589 4385 4478 2589
Size: 1796 93 4985
Fragments arranged by size:
4985 1796 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2125 2668 3135 4193 4935 5020 6091
Size: 399 335 543 467 1058 742 85 1071
Cuts at: 6091 1391
Size: 2174
Fragments arranged by size:
2174 1071 1058 742 543 467 399 335
85
BspEI T'CCGG_A
Cuts at: 2425 6537 2425
Size: 4112 2762
BspHI T'CATG_A
Cuts at: 4066 4763 6493 4066
Size: 697 1730 4447
Fragments arranged by size:
4447 1730 697
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4229 844
Size: 3385 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4761 1394
Size: 429 2938 3507
Fragments arranged by size:
3507 2938 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2473 3450 3955 3996 5369 5543 65
Size: 1183 1225 977 505 41 1373 174 1396
Fragments arranged by size:
1396 1373 1225 1183 977 505 174 41
BsrFI r'CCGG_y
Cuts at: 1700 1810 5518 5705 1700
Size: 110 3708 187 2869
Fragments arranged by size:
3708 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3562 96
Size: 3466 3408
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6874
BssSI C'ACGA_G
Cuts at: 4934 5946 4934
Size: 1012 5862
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6874
BstBI TT'CG_AA
Cuts at: 5678 5678
Size: 6874
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6874
BstYI r'GATC_y
Cuts at: 1972 4127 4204 4571 4956 4973 6425 6511 6523
Size: 2155 77 367 385 17 1452 86 12
Cuts at: 6523 6869 1972
Size: 346 1977
Fragments arranged by size:
2155 1977 1452 385 367 346 86 77
17 12
BstZ17I GTA'TAC
Cuts at: 4117 4117
Size: 6874
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5383
Fragments arranged by size:
5383 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6135
Fragments arranged by size:
6135 683 56
BtsI GCAGTG_nn'
Cuts at: 2409 4390 5184 5204 2409
Size: 1981 794 20 4079
Fragments arranged by size:
4079 1981 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6874
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3998 4033 529
Size: 35 3434 35 3370
Fragments arranged by size:
3434 3370 35 35
DraI TTT'AAA
Cuts at: 2712 4178 2712
Size: 1466 5408
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5881 809
Size: 507 4565 1802
Fragments arranged by size:
4565 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3597 4006 4321
Size: 54 1425 50 275 18 1767 409 315
Cuts at: 4321 5210 6839 8
Size: 889 1629 43
Fragments arranged by size:
1767 1629 1425 889 409 315 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4321 1537
Size: 275 2509 4090
Fragments arranged by size:
4090 2509 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2082 4804 1353
Size: 729 2722 3423
Fragments arranged by size:
3423 2722 729
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 5461 5835 5981 387
Size: 1064 138 435 3437 374 146 1280
Fragments arranged by size:
3437 1280 1064 435 374 146 138
EcoICRI GAG'CTC
Cuts at: 2123 2123
Size: 6874
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2046 2408 2987 3371 3714 4937 5534
Size: 393 414 362 579 384 343 1223 597
Cuts at: 5534 6321 6589 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1223 787 597 579 414 393 384
362 343 268
EcoO109I rG'GnC_Cy
Cuts at: 3959 3959
Size: 6874
EcoRI G'AATT_C
Cuts at: 3677 4183 4561 3677
Size: 506 378 5990
Fragments arranged by size:
5990 506 378
EcoRV GAT'ATC
Cuts at: 4161 4161
Size: 6874
FspI TGC'GCA
Cuts at: 5380 5380
Size: 6874
HaeII r_GCGC'y
Cuts at: 1678 6021 1678
Size: 4343 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2188 2220 5515 5547 5589
Size: 32 135 32 616 32 3295 32 42
Cuts at: 5589 5621 1373
Size: 32 2626
Fragments arranged by size:
3295 2626 616 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4218 4464 669
Size: 988 281 2280 246 3079
Fragments arranged by size:
3079 2280 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6874
HpaI GTT'AAC
Cuts at: 4464 4464
Size: 6874
KpnI G_GTAC'C
Cuts at: 4199 4199
Size: 6874
MfeI C'AATT_G
Cuts at: 4473 4473
Size: 6874
MluI A'CGCG_T
Cuts at: 6750 6750
Size: 6874
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2271 5988 6172 1305
Size: 966 3717 184 2007
Fragments arranged by size:
3717 2007 966 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4008 10
Size: 54 1425 343 2176 2876
Fragments arranged by size:
2876 2176 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2780 3284 3599 4832 5191 5350 518
Size: 648 1614 504 315 1233 359 159 2042
Fragments arranged by size:
2042 1614 1233 648 504 359 315 159
MspA1I CmG'CkG
Cuts at: 1606 2180 3007 3740 4290 4967 6115 6360 1606
Size: 574 827 733 550 677 1148 245 2120
Fragments arranged by size:
2120 1148 827 733 677 574 550 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5383
Fragments arranged by size:
5383 939 552
NdeI CA'TA_TG
Cuts at: 387 4093 387
Size: 3706 3168
NheI G'CTAG_C
Cuts at: 1052 2438 2575 1052
Size: 1386 137 5351
Fragments arranged by size:
5351 1386 137
NotI GC'GGCC_GC
Cuts at: 4321 4321
Size: 6874
NspI r_CATG'y
Cuts at: 1132 1229 2135 2685 3139 3493 4232 5777 1132
Size: 97 906 550 454 354 739 1545 2229
Fragments arranged by size:
2229 1545 906 739 550 454 354 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6874
PciI A'CATG_T
Cuts at: 2131 5773 2131
Size: 3642 3232
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6874
PmeI GTTT'AAAC
Cuts at: 4178 4178
Size: 6874
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4943 4975 6456 6488 4943
Size: 32 1481 32 5329
Fragments arranged by size:
5329 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3959 3959
Size: 6874
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6874
PsiI TTA'TAA
Cuts at: 2313 2889 3098 3894 4444 2313
Size: 576 209 796 550 4743
Fragments arranged by size:
4743 796 576 550 209
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 4115 4147 4115
Size: 32 6842
PstI C_TGCA'G
Cuts at: 830 3222 4226 830
Size: 2392 1004 3478
Fragments arranged by size:
3478 2392 1004
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6874
PvuII CAG'CTG
Cuts at: 3007 3740 3007
Size: 733 6141
SacI G_AGCT'C
Cuts at: 2125 2125
Size: 6874
SalI G'TCGA_C
Cuts at: 1936 4216 1936
Size: 2280 4594
SanDI GG'GwC_CC
Cuts at: 3959 3959
Size: 6874
SbfI CC_TGCA'GG
Cuts at: 3222 4226 3222
Size: 1004 5870
ScaI AGT'ACT
Cuts at: 5122 6834 5122
Size: 1712 5162
SfcI C'TryA_G
Cuts at: 826 1045 2028 2927 3218 3671 3973 4222 5357
Size: 219 983 899 291 453 302 249 1135
Cuts at: 5357 6038 6229 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 983 899 681 453 302 291
249 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6874
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6874
SmaI CCC'GGG
Cuts at: 1872 4201 1872
Size: 2329 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3231 3836 4978 5879 6141 820
Size: 197 926 1288 605 1142 901 262 1553
Fragments arranged by size:
1553 1288 1142 926 901 605 262 197
SnaBI TAC'GTA
Cuts at: 493 4665 493
Size: 4172 2702
SpeI A'CTAG_T
Cuts at: 152 2186 152
Size: 2034 4840
SphI G_CATG'C
Cuts at: 1132 2685 4232 1132
Size: 1553 1547 3774
Fragments arranged by size:
3774 1553 1547
SspI AAT'ATT
Cuts at: 5 52 3094 3573 4798 5
Size: 47 3042 479 1225 2081
Fragments arranged by size:
3042 2081 1225 479 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6874
StyI C'CwwG_G
Cuts at: 513 1065 2004 4313 513
Size: 552 939 2309 3074
Fragments arranged by size:
3074 2309 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3582 3758 4901 4918 5071 5256 5701
Size: 1255 2002 176 1143 17 153 185 445
Cuts at: 5701 325
Size: 1498
Fragments arranged by size:
2002 1498 1255 1143 445 185 176 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3071 3465 3562
Size: 274 80 33 51 1443 1094 394 97
Cuts at: 3562 5120 6832 96
Size: 1558 1712 138
Fragments arranged by size:
1712 1558 1443 1094 394 274 138 97
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3783 3815 6834 6866 3783
Size: 32 3019 32 3791
Fragments arranged by size:
3791 3019 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3256 1316
Size: 1940 4934
XbaI T'CTAG_A
Cuts at: 4210 4210
Size: 6874
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6874
XmaI C'CCGG_G
Cuts at: 1870 4199 1870
Size: 2329 4545
XmnI GAAnn'nnTTC
Cuts at: 4103 5003 4103
Size: 900 5974
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4682 276
Size: 53 83 186 936 3148 2468
Fragments arranged by size:
3148 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI
PsrI PstI PvuI PvuII SacI SalI SanDI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BplI BsiWI BsmBI BstXI Bsu36I DraIII EcoNI
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacII
SapI SexAI SfiI SfoI SrfI SwaI XcmI