Restriction map for
pFN21AE0010
(Circular) (Six-base) MAPSORT of: pFN21AE0010.seq Check: 3188 from: 1 to: 7015
pFN21AE0010 7015 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3030 3030
Size: 7015
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4825 278
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3091 4358 1937
Size: 1154 1267 4594
Fragments arranged by size:
4594 1267 1154
Acc65I G'GTAC_C
Cuts at: 4336 4336
Size: 7015
AclI AA'CG_TT
Cuts at: 1563 5142 5515 1563
Size: 3579 373 3063
Fragments arranged by size:
3579 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2412 3363 5078 6462 6730 1632
Size: 780 951 1715 1384 268 1917
Fragments arranged by size:
1917 1715 1384 951 780 268
AflII C'TTAA_G
Cuts at: 820 1017 3709 820
Size: 197 2692 4126
Fragments arranged by size:
4126 2692 197
AflIII A'CryG_T
Cuts at: 1203 5914 6891 1203
Size: 4711 977 1327
Fragments arranged by size:
4711 1327 977
AgeI A'CCGG_T
Cuts at: 5846 5846
Size: 7015
AhdI GACnn_n'nnGTC
Cuts at: 5744 5744
Size: 7015
AleI CACnn'nnGTG
Cuts at: 1166 4037 1166
Size: 2871 4144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7015
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2379 2411 4142 4174 2379
Size: 32 1731 32 5220
Fragments arranged by size:
5220 1731 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2068 2379 2447 2629 3341 4115 4270 4758 6330
Size: 311 68 182 712 774 155 488 1572
Cuts at: 6330 2068
Size: 2753
Fragments arranged by size:
2753 1572 774 712 488 311 182 155
68
ApaLI G'TGCA_C
Cuts at: 5072 6228 5072
Size: 1156 5859
ApoI r'AATT_y
Cuts at: 1493 2368 3687 4324 4550 4702 6811 1493
Size: 875 1319 637 226 152 2109 1697
Fragments arranged by size:
2109 1697 1319 875 637 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5569 160
Size: 872 4537 1606
Fragments arranged by size:
4537 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
AvaI C'yCGr_G
Cuts at: 1870 1943 4340 1870
Size: 73 2397 4545
Fragments arranged by size:
4545 2397 73
BamHI G'GATC_C
Cuts at: 3199 4345 4712 3199
Size: 1146 367 5502
Fragments arranged by size:
5502 1146 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2582 4336 5791 618
Size: 325 217 627 795 1754 1455 1842
Fragments arranged by size:
1842 1754 1455 795 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3446 4334 1391
Size: 2055 888 4072
Fragments arranged by size:
4072 2055 888
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4101 928
Size: 971 2202 3842
Fragments arranged by size:
3842 2202 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5206 5240 1903
Size: 34 3269 34 3678
Fragments arranged by size:
3678 3269 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3782 4302 4907 6117 3782
Size: 520 605 1210 4680
Fragments arranged by size:
4680 1210 605 520
BclI T'GATC_A
Cuts at: 1548 2092 1548
Size: 544 6471
BfrBI ATG'CAT
Cuts at: 2057 2057
Size: 7015
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2473 5626 136
Size: 107 122 71 631 605 801 3153 1525
Fragments arranged by size:
3153 1525 801 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3035 7010 3035
Size: 3975 3040
BlpI GC'TnA_GC
Cuts at: 4432 4432
Size: 7015
Bme1580I G_kGCm'C
Cuts at: 1790 5076 6232 1790
Size: 3286 1156 2573
Fragments arranged by size:
3286 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7015
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2078 4746 5704
Size: 416 53 83 399 24 661 2668 958
Cuts at: 5704 442
Size: 1753
Fragments arranged by size:
2668 1753 958 661 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7015
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2718 2718
Size: 7015
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2771 3344 3431 4028 4301 5675 1239
Size: 1532 573 87 597 273 1374 2579
Fragments arranged by size:
2579 1532 1374 597 573 273 87
Bpu10I CC'TnA_GC
Cuts at: 2427 4082 2427
Size: 1655 5360
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5140 6005 6303 5140
Size: 865 298 5852
Fragments arranged by size:
5852 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3247 5678 882
Size: 617 1748 2431 2219
Fragments arranged by size:
2431 2219 1748 617
BsaAI yAC'GTr
Cuts at: 493 1204 4806 493
Size: 711 3602 2702
Fragments arranged by size:
3602 2702 711
BsaBI GATnn'nnATC
Cuts at: 7009 7009
Size: 7015
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4822 5204 5839 275
Size: 53 83 186 936 3289 382 635 1451
Fragments arranged by size:
3289 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3058 3168 5448 5846 6120 6267 6678 3058
Size: 110 2280 398 274 147 411 3395
Fragments arranged by size:
3395 2280 411 398 274 147 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2971 3001 3317 3347 3618
Size: 30 165 30 1568 30 316 30 271
Cuts at: 3618 3648 4142 4172 1178
Size: 30 494 30 4021
Fragments arranged by size:
4021 1568 494 316 271 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2267 2823 3574 4061 1186
Size: 1081 556 751 487 4140
Fragments arranged by size:
4140 1081 751 556 487
BseYI C'CCAG_C
Cuts at: 2609 2840 2935 6218 6714 2609
Size: 231 95 3283 496 2910
Fragments arranged by size:
3283 2910 496 231 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2145 2348 4020 1163
Size: 573 409 203 1672 4158
Fragments arranged by size:
4158 1672 573 409 203
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4465 5226 6254 1540
Size: 275 186 2464 761 1028 2301
Fragments arranged by size:
2464 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2797 2815 2968 3417 4044 5076 5161 6232 2797
Size: 18 153 449 627 1032 85 1071 3580
Fragments arranged by size:
3580 1071 1032 627 449 153 85 18
BsmI GAATG_Cn'
Cuts at: 4526 4619 4526
Size: 93 6922
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2797 2815 2968 3417 3446 4044 4334
Size: 399 1007 18 153 449 29 598 290
Cuts at: 4334 5076 5161 6232 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 1007 742 598 449 399 290
153 85 29 18
BspEI T'CCGG_A
Cuts at: 6678 6678
Size: 7015
BspHI T'CATG_A
Cuts at: 3843 4904 6634 3843
Size: 1061 1730 4224
Fragments arranged by size:
4224 1730 1061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3030 3667 4370 844
Size: 2186 637 703 3489
Fragments arranged by size:
3489 2186 703 637
BsrBI CCG'CTC
Cuts at: 1394 1823 4902 1394
Size: 429 3079 3507
Fragments arranged by size:
3507 3079 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2218 5510 5684 65
Size: 1183 970 3292 174 1396
Fragments arranged by size:
3292 1396 1183 970 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4260 5659 5846 1700
Size: 110 2450 1399 187 2869
Fragments arranged by size:
2869 2450 1399 187 110
BsrGI T'GTAC_A
Cuts at: 96 3757 96
Size: 3661 3354
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7015
BssSI C'ACGA_G
Cuts at: 4170 5075 6087 4170
Size: 905 1012 5098
Fragments arranged by size:
5098 1012 905
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 7015
BstBI TT'CG_AA
Cuts at: 5819 5819
Size: 7015
BstEII G'GTnAC_C
Cuts at: 1184 2243 2744 1184
Size: 1059 501 5455
Fragments arranged by size:
5455 1059 501
BstYI r'GATC_y
Cuts at: 1972 2305 2387 3035 3199 4345 4712 5097 5114
Size: 333 82 648 164 1146 367 385 17
Cuts at: 5114 6566 6652 6664 7010 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1146 648 385 367 346 333
164 86 82 17 12
Bsu36I CC'TnA_GG
Cuts at: 3396 3396
Size: 7015
BtgI C'CryG_G
Cuts at: 513 1065 4087 513
Size: 552 3022 3441
Fragments arranged by size:
3441 3022 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6276
Fragments arranged by size:
6276 683 56
BtsI GCAGTG_nn'
Cuts at: 2331 2417 2725 4032 4064 4531 5325 5345 2331
Size: 86 308 1307 32 467 794 20 4001
Fragments arranged by size:
4001 1307 794 467 308 86 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7015
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6980
DraI TTT'AAA
Cuts at: 4319 4319
Size: 7015
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6022 809
Size: 507 4706 1802
Fragments arranged by size:
4706 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3402 4203 4462
Size: 54 1425 50 275 18 1572 801 259
Cuts at: 4462 5351 6980 8
Size: 889 1629 43
Fragments arranged by size:
1629 1572 1425 889 801 275 259 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4462 1537
Size: 275 2650 4090
Fragments arranged by size:
4090 2650 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2079 2737 3645 3779 4945 1353
Size: 726 658 908 134 1166 3423
Fragments arranged by size:
3423 1166 908 726 658 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5602 5976 6122 387
Size: 1064 138 4013 374 146 1280
Fragments arranged by size:
4013 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2412 2771 3344 3363 3431 4028 4301
Size: 393 780 359 573 19 68 597 273
Cuts at: 4301 5078 5675 6462 6730 1239
Size: 777 597 787 268 1524
Fragments arranged by size:
1524 787 780 777 597 597 573 393
359 273 268 68 19
EcoNI CCTnn'n_nnAGG
Cuts at: 3009 3009
Size: 7015
EcoO109I rG'GnC_Cy
Cuts at: 2475 2503 2857 3014 3043 2475
Size: 28 354 157 29 6447
Fragments arranged by size:
6447 354 157 29 28
EcoRI G'AATT_C
Cuts at: 2368 4324 4702 2368
Size: 1956 378 4681
Fragments arranged by size:
4681 1956 378
FspI TGC'GCA
Cuts at: 5521 5521
Size: 7015
HaeII r_GCGC'y
Cuts at: 1678 2161 6162 1678
Size: 483 4001 2531
Fragments arranged by size:
4001 2531 483
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2617 2649 2684 2716 3163
Size: 32 135 32 1045 32 35 32 447
Cuts at: 3163 3195 3227 3259 3317 3349 3618 3650 5656
Size: 32 32 32 58 32 269 32 2006
Cuts at: 5656 5688 5730 5762 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 2006 1045 447 269 135 58 42
35 32 32 32 32 32 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2535 3092 4258 4359 4605 669
Size: 988 281 597 557 1166 101 246 3079
Fragments arranged by size:
3079 1166 988 597 557 281 246 101
HindIII A'AGCT_T
Cuts at: 748 3383 3455 748
Size: 2635 72 4308
Fragments arranged by size:
4308 2635 72
HpaI GTT'AAC
Cuts at: 4605 4605
Size: 7015
KpnI G_GTAC'C
Cuts at: 4340 4340
Size: 7015
MfeI C'AATT_G
Cuts at: 4614 4614
Size: 7015
MluI A'CGCG_T
Cuts at: 6891 6891
Size: 7015
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 6129 6313 1305
Size: 4824 184 2007
Fragments arranged by size:
4824 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3404 4205 10
Size: 54 1425 343 1572 801 2820
Fragments arranged by size:
2820 1572 1425 801 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3842 4037 4973 5332 5491 518
Size: 648 2676 195 936 359 159 2042
Fragments arranged by size:
2676 2042 936 648 359 195 159
MspA1I CmG'CkG
Cuts at: 1606 3226 3338 3833 4431 5108 6256 6501 1606
Size: 1620 112 495 598 677 1148 245 2120
Fragments arranged by size:
2120 1620 1148 677 598 495 245 112
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6463
NdeI CA'TA_TG
Cuts at: 387 3205 387
Size: 2818 4197
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7015
NotI GC'GGCC_GC
Cuts at: 4462 4462
Size: 7015
NsiI A_TGCA'T
Cuts at: 2059 2059
Size: 7015
NspI r_CATG'y
Cuts at: 1132 1229 3700 4373 5918 1132
Size: 97 2471 673 1545 2229
Fragments arranged by size:
2471 2229 1545 673 97
PasI CC'CwG_GG
Cuts at: 1348 2482 1348
Size: 1134 5881
PciI A'CATG_T
Cuts at: 5914 5914
Size: 7015
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2379 1108
Size: 1271 5744
PmeI GTTT'AAAC
Cuts at: 4319 4319
Size: 7015
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4142 4174 5084 5116 6597 6629 4142
Size: 32 910 32 1481 32 4528
Fragments arranged by size:
4528 1481 910 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2503 2857 3014 2503
Size: 354 157 6504
Fragments arranged by size:
6504 354 157
PshAI GACnn'nnGTC
Cuts at: 1613 2833 1613
Size: 1220 5795
PsiI TTA'TAA
Cuts at: 3578 4585 3578
Size: 1007 6008
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2505 2537 2505
Size: 32 6983
PstI C_TGCA'G
Cuts at: 830 2720 4296 4367 830
Size: 1890 1576 71 3478
Fragments arranged by size:
3478 1890 1576 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7015
PvuII CAG'CTG
Cuts at: 3338 3833 3338
Size: 495 6520
SalI G'TCGA_C
Cuts at: 1936 3090 4357 1936
Size: 1154 1267 4594
Fragments arranged by size:
4594 1267 1154
SanDI GG'GwC_CC
Cuts at: 2857 2857
Size: 7015
SapI GCTCTTCn'nnn_
Cuts at: 3645 3645
Size: 7015
SbfI CC_TGCA'GG
Cuts at: 4367 4367
Size: 7015
ScaI AGT'ACT
Cuts at: 5263 6975 5263
Size: 1712 5303
SfcI C'TryA_G
Cuts at: 826 1045 2439 2716 2819 3592 4100 4119 4292
Size: 219 1394 277 103 773 508 19 173
Cuts at: 4292 4363 5498 6179 6370 826
Size: 71 1135 681 191 1471
Fragments arranged by size:
1471 1394 1135 773 681 508 277 219
191 173 103 71 19
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7015
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7015
SmaI CCC'GGG
Cuts at: 1872 4342 1872
Size: 2470 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3709 5119 6020 6282 820
Size: 197 926 1766 1410 901 262 1553
Fragments arranged by size:
1766 1553 1410 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4806 493
Size: 4313 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7015
SphI G_CATG'C
Cuts at: 1132 4373 1132
Size: 3241 3774
SspI AAT'ATT
Cuts at: 5 52 4939 5
Size: 47 4887 2081
Fragments arranged by size:
4887 2081 47
StuI AGG'CCT
Cuts at: 1517 3739 1517
Size: 2222 4793
StyI C'CwwG_G
Cuts at: 513 1065 2525 4454 513
Size: 552 1460 1929 3074
Fragments arranged by size:
3074 1929 1460 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2231 2633 5042 5059 5212 5397 5842
Size: 1255 651 402 2409 17 153 185 445
Cuts at: 5842 325
Size: 1498
Fragments arranged by size:
2409 1498 1255 651 445 402 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3757 5261 6973
Size: 274 80 33 51 1443 1780 1504 1712
Cuts at: 6973 96
Size: 138
Fragments arranged by size:
1780 1712 1504 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2931 2963 6975 7007 2931
Size: 32 4012 32 2939
Fragments arranged by size:
4012 2939 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 7015
XbaI T'CTAG_A
Cuts at: 4351 4351
Size: 7015
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 4052 4052
Size: 7015
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7015
XmaI C'CCGG_G
Cuts at: 1870 4340 1870
Size: 2470 4545
XmnI GAAnn'nnTTC
Cuts at: 5144 5144
Size: 7015
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4823 276
Size: 53 83 186 936 3289 2468
Fragments arranged by size:
3289 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NsiI NspI PasI PciI PflMI PmeI PpiI
PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI
PmlI PspOMI PspXI RsrII SacI SacII SexAI SfiI
SfoI SrfI SwaI