Restriction map for
pFN21AE0009
(Circular) (Six-base) MAPSORT of: pFN21AE0009.seq Check: 7833 from: 1 to: 6634
pFN21AE0009 6634 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4444 278
Size: 53 83 186 936 2908 2468
Fragments arranged by size:
2908 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3977 1937
Size: 2040 4594
Acc65I G'GTAC_C
Cuts at: 3955 3955
Size: 6634
AclI AA'CG_TT
Cuts at: 1563 4761 5134 1563
Size: 3198 373 3063
Fragments arranged by size:
3198 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2472 3066 3134 3200 4697 6081 6349 1632
Size: 840 594 68 66 1497 1384 268 1917
Fragments arranged by size:
1917 1497 1384 840 594 268 68 66
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6437
AflIII A'CryG_T
Cuts at: 1203 5533 6510 1203
Size: 4330 977 1327
Fragments arranged by size:
4330 1327 977
AgeI A'CCGG_T
Cuts at: 5465 5465
Size: 6634
AhdI GACnn_n'nnGTC
Cuts at: 5363 5363
Size: 6634
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6634
AlwNI CAG_nnn'CTG
Cuts at: 3884 4377 5949 3884
Size: 493 1572 4569
Fragments arranged by size:
4569 1572 493
ApaLI G'TGCA_C
Cuts at: 4691 5847 4691
Size: 1156 5478
ApoI r'AATT_y
Cuts at: 1493 2076 2166 3040 3362 3444 3943 4169 4321
Size: 583 90 874 322 82 499 226 152
Cuts at: 4321 6430 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 874 583 499 322 226 152
90 82
AseI AT'TA_AT
Cuts at: 160 1032 5188 160
Size: 872 4156 1606
Fragments arranged by size:
4156 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6634
AvaI C'yCGr_G
Cuts at: 1870 1943 3959 1870
Size: 73 2016 4545
Fragments arranged by size:
4545 2016 73
AvrII C'CTAG_G
Cuts at: 2081 2081
Size: 6634
BamHI G'GATC_C
Cuts at: 3648 3964 4331 3648
Size: 316 367 5951
Fragments arranged by size:
5951 367 316
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2430 3955 5410 618
Size: 325 217 627 643 1525 1455 1842
Fragments arranged by size:
1842 1525 1455 643 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2364 2988 3953 1391
Size: 973 624 965 4072
Fragments arranged by size:
4072 973 965 624
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3377 928
Size: 971 1478 4185
Fragments arranged by size:
4185 1478 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2983 3017 4825 4859 1903
Size: 34 1046 34 1808 34 3678
Fragments arranged by size:
3678 1808 1046 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3810 4526 5736 3810
Size: 716 1210 4708
Fragments arranged by size:
4708 1210 716
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6634
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5245 136
Size: 107 122 71 631 605 3573 1525
Fragments arranged by size:
3573 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2100 6629 2100
Size: 4529 2105
BlpI GC'TnA_GC
Cuts at: 4051 4051
Size: 6634
Bme1580I G_kGCm'C
Cuts at: 1790 2435 4695 5851 1790
Size: 645 2260 1156 2573
Fragments arranged by size:
2573 2260 1156 645
BmgBI CAC'GTC
Cuts at: 1318 3586 1318
Size: 2268 4366
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4365 5323 442
Size: 416 53 83 399 24 2948 958 1753
Fragments arranged by size:
2948 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6634
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2286 2721 3002 5294 1239
Size: 1047 435 281 2292 2579
Fragments arranged by size:
2579 2292 1047 435 281
Bpu10I CC'TnA_GC
Cuts at: 2358 2358
Size: 6634
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4759 5624 5922 4759
Size: 865 298 5471
Fragments arranged by size:
5471 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2618 5297 882
Size: 617 1119 2679 2219
Fragments arranged by size:
2679 2219 1119 617
BsaAI yAC'GTr
Cuts at: 493 1204 4425 493
Size: 711 3221 2702
Fragments arranged by size:
3221 2702 711
BsaBI GATnn'nnATC
Cuts at: 6628 6628
Size: 6634
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4441 4823 5458 275
Size: 53 83 186 936 2908 382 635 1451
Fragments arranged by size:
2908 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5067 5465 5739 5886 6297 5067
Size: 398 274 147 411 5404
Fragments arranged by size:
5404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 3725 3755 1178
Size: 30 165 30 822 30 1470 30 4057
Fragments arranged by size:
4057 1470 822 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2520 2594 2691 2694 2720 2725 2762 2765
Size: 1334 74 97 3 26 5 37 3
Cuts at: 2765 2768 2777 2780 2783 2792 2801 2810 2819
Size: 3 9 3 3 9 9 9 9
Cuts at: 2819 2835 2853 1186
Size: 16 18 4967
Fragments arranged by size:
4967 1334 97 74 37 26 18 16
9 9 9 9 9 5 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 5837 6333 5837
Size: 496 6138
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2350 3599 1163
Size: 573 614 1249 4198
Fragments arranged by size:
4198 1249 614 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4084 4845 5873 1540
Size: 275 186 2083 761 1028 2301
Fragments arranged by size:
2301 2083 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2900 2988 4695 4780 5851 2900
Size: 88 1707 85 1071 3683
Fragments arranged by size:
3683 1707 1071 88 85
BsmI GAATG_Cn'
Cuts at: 2465 2841 3101 4145 4238 2465
Size: 376 260 1044 93 4861
Fragments arranged by size:
4861 1044 376 260 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2364 2435 2900 2988 3953 4695 4780
Size: 399 574 71 465 88 965 742 85
Cuts at: 4780 5851 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 965 742 574 465 399 88
85 71
BspEI T'CCGG_A
Cuts at: 6297 6297
Size: 6634
BspHI T'CATG_A
Cuts at: 4523 6253 4523
Size: 1730 4904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2381 3989 844
Size: 1537 1608 3489
Fragments arranged by size:
3489 1608 1537
BsrBI CCG'CTC
Cuts at: 1394 1823 4521 1394
Size: 429 2698 3507
Fragments arranged by size:
3507 2698 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5129 5303 65
Size: 1183 3881 174 1396
Fragments arranged by size:
3881 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5278 5465 1700
Size: 110 3468 187 2869
Fragments arranged by size:
3468 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2334 96
Size: 2238 4396
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6634
BssSI C'ACGA_G
Cuts at: 4694 5706 4694
Size: 1012 5622
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2373 794
Size: 1579 5055
BstBI TT'CG_AA
Cuts at: 5438 5438
Size: 6634
BstEII G'GTnAC_C
Cuts at: 1184 3259 1184
Size: 2075 4559
BstYI r'GATC_y
Cuts at: 1972 2100 3648 3964 4331 4716 4733 6185 6271
Size: 128 1548 316 367 385 17 1452 86
Cuts at: 6271 6283 6629 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1548 1452 385 367 346 316 128
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2532 2532
Size: 6634
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 6082
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2986 487
Size: 683 56 1760 4135
Fragments arranged by size:
4135 1760 683 56
BtsI GCAGTG_nn'
Cuts at: 2876 4150 4944 4964 2876
Size: 1274 794 20 4546
Fragments arranged by size:
4546 1274 794 20
ClaI AT'CG_AT
Cuts at: 1554 3225 1554
Size: 1671 4963
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6599
DraI TTT'AAA
Cuts at: 3938 3938
Size: 6634
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5641 809
Size: 507 4325 1802
Fragments arranged by size:
4325 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3667 4081 4970
Size: 54 1425 50 275 18 1837 414 889
Cuts at: 4970 6599 8
Size: 1629 43
Fragments arranged by size:
1837 1629 1425 889 414 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4081 1537
Size: 275 2269 4090
Fragments arranged by size:
4090 2269 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2386 3323 4564 1353
Size: 1033 937 1241 3423
Fragments arranged by size:
3423 1241 1033 937
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5221 5595 5741 387
Size: 1064 138 3632 374 146 1280
Fragments arranged by size:
3632 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2986 2986
Size: 6634
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2286 2472 2721 3002 3066 3134 3200
Size: 393 654 186 249 281 64 68 66
Cuts at: 3200 4697 5294 6081 6349 1239
Size: 1497 597 787 268 1524
Fragments arranged by size:
1524 1497 787 654 597 393 281 268
249 186 68 66 64
EcoNI CCTnn'n_nnAGG
Cuts at: 2868 2868
Size: 6634
EcoO109I rG'GnC_Cy
Cuts at: 2809 2809
Size: 6634
EcoRI G'AATT_C
Cuts at: 2166 3943 4321 2166
Size: 1777 378 4479
Fragments arranged by size:
4479 1777 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2585 2585
Size: 6634
FspI TGC'GCA
Cuts at: 5140 5140
Size: 6634
HaeII r_GCGC'y
Cuts at: 1678 5781 1678
Size: 4103 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2962 2994 3725 3757 3785
Size: 32 135 32 1390 32 731 32 28
Cuts at: 3785 3817 5275 5307 5349 5381 1373
Size: 32 1458 32 42 32 2626
Fragments arranged by size:
2626 1458 1390 731 135 42 32 32
32 32 32 32 32 28
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 3978 4224 669
Size: 988 281 277 1763 246 3079
Fragments arranged by size:
3079 1763 988 281 277 246
HindIII A'AGCT_T
Cuts at: 748 3107 748
Size: 2359 4275
HpaI GTT'AAC
Cuts at: 2215 4224 2215
Size: 2009 4625
KpnI G_GTAC'C
Cuts at: 3959 3959
Size: 6634
MfeI C'AATT_G
Cuts at: 3170 4233 3170
Size: 1063 5571
MluI A'CGCG_T
Cuts at: 6510 6510
Size: 6634
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5748 5932 1305
Size: 4443 184 2007
Fragments arranged by size:
4443 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3669 10
Size: 54 1425 343 1837 2975
Fragments arranged by size:
2975 1837 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2330 4592 4951 5110 518
Size: 648 1164 2262 359 159 2042
Fragments arranged by size:
2262 2042 1164 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2922 2956 4050 4727 5875 6120 1606
Size: 1316 34 1094 677 1148 245 2120
Fragments arranged by size:
2120 1316 1148 1094 677 245 34
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6082
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6634
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6634
NotI GC'GGCC_GC
Cuts at: 4081 4081
Size: 6634
NspI r_CATG'y
Cuts at: 1132 1229 3992 5537 1132
Size: 97 2763 1545 2229
Fragments arranged by size:
2763 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6634
PciI A'CATG_T
Cuts at: 5533 5533
Size: 6634
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6634
PfoI T'CCnGG_A
Cuts at: 2263 2263
Size: 6634
PmeI GTTT'AAAC
Cuts at: 3938 3938
Size: 6634
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2110 2142 4703 4735 6216 6248 2110
Size: 32 2561 32 1481 32 2496
Fragments arranged by size:
2561 2496 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 2809
Size: 6634
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6634
PsiI TTA'TAA
Cuts at: 2283 3492 4204 2283
Size: 1209 712 4713
Fragments arranged by size:
4713 1209 712
PstI C_TGCA'G
Cuts at: 830 3986 830
Size: 3156 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6634
PvuII CAG'CTG
Cuts at: 2922 2922
Size: 6634
SacI G_AGCT'C
Cuts at: 2988 2988
Size: 6634
SalI G'TCGA_C
Cuts at: 1936 3976 1936
Size: 2040 4594
SapI GCTCTTCn'nnn_
Cuts at: 3323 3323
Size: 6634
SbfI CC_TGCA'GG
Cuts at: 3986 3986
Size: 6634
ScaI AGT'ACT
Cuts at: 3624 4882 6594 3624
Size: 1258 1712 3664
Fragments arranged by size:
3664 1712 1258
SfcI C'TryA_G
Cuts at: 826 1045 2908 3982 5117 5798 5989 826
Size: 219 1863 1074 1135 681 191 1471
Fragments arranged by size:
1863 1471 1135 1074 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6634
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6634
SmaI CCC'GGG
Cuts at: 1872 3961 1872
Size: 2089 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4738 5639 5901 820
Size: 197 926 2795 901 262 1553
Fragments arranged by size:
2795 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4425 493
Size: 3932 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6634
SphI G_CATG'C
Cuts at: 1132 3992 1132
Size: 2860 3774
SspI AAT'ATT
Cuts at: 5 52 4558 5
Size: 47 4506 2081
Fragments arranged by size:
4506 2081 47
StuI AGG'CCT
Cuts at: 1517 2698 3684 1517
Size: 1181 986 4467
Fragments arranged by size:
4467 1181 986
StyI C'CwwG_G
Cuts at: 513 1065 2081 2737 3628 4073 513
Size: 552 1016 656 891 445 3074
Fragments arranged by size:
3074 1016 891 656 552 445
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2562 4661 4678 4831 5016 5461 325
Size: 1255 982 2099 17 153 185 445 1498
Fragments arranged by size:
2099 1498 1255 982 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2334 3481 3622
Size: 274 80 33 51 1443 357 1147 141
Cuts at: 3622 4880 6592 96
Size: 1258 1712 138
Fragments arranged by size:
1712 1443 1258 1147 357 274 141 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3575 3607 6594 6626 3575
Size: 32 2987 32 3583
Fragments arranged by size:
3583 2987 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3737 1316
Size: 2421 4213
XbaI T'CTAG_A
Cuts at: 2636 3547 3889 3970 2636
Size: 911 342 81 5300
Fragments arranged by size:
5300 911 342 81
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6634
XmaI C'CCGG_G
Cuts at: 1870 3959 1870
Size: 2089 4545
XmnI GAAnn'nnTTC
Cuts at: 2076 2568 3777 4763 2076
Size: 492 1209 986 3947
Fragments arranged by size:
3947 1209 986 492
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4442 276
Size: 53 83 186 936 2908 2468
Fragments arranged by size:
2908 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI