Restriction map for
pFN21AE0005
(Circular) (Six-base) MAPSORT of: pFN21AE0005.seq Check: 6642 from: 1 to: 6808
pFN21AE0005 6808 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4618 278
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4151 1937
Size: 2214 4594
Acc65I G'GTAC_C
Cuts at: 3843 4129 3843
Size: 286 6522
AclI AA'CG_TT
Cuts at: 1563 4935 5308 1563
Size: 3372 373 3063
Fragments arranged by size:
3372 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2658 3374 3765 4103 4871 6255 6523 1632
Size: 1026 716 391 338 768 1384 268 1917
Fragments arranged by size:
1917 1384 1026 768 716 391 338 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6611
AflIII A'CryG_T
Cuts at: 1203 3878 5707 6684 1203
Size: 2675 1829 977 1327
Fragments arranged by size:
2675 1829 1327 977
AgeI A'CCGG_T
Cuts at: 5639 5639
Size: 6808
AhdI GACnn_n'nnGTC
Cuts at: 5537 5537
Size: 6808
AleI CACnn'nnGTG
Cuts at: 1166 2862 1166
Size: 1696 5112
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6808
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3306 3338 3306
Size: 32 6776
AlwNI CAG_nnn'CTG
Cuts at: 4551 6123 4551
Size: 1572 5236
ApaLI G'TGCA_C
Cuts at: 4865 6021 4865
Size: 1156 5652
ApoI r'AATT_y
Cuts at: 1493 2483 2549 2686 2693 3196 3482 3615 3712
Size: 990 66 137 7 503 286 133 97
Cuts at: 3712 3729 4117 4343 4495 6604 1493
Size: 17 388 226 152 2109 1697
Fragments arranged by size:
2109 1697 990 503 388 286 226 152
137 133 97 66 17 7
AseI AT'TA_AT
Cuts at: 160 1032 5362 160
Size: 872 4330 1606
Fragments arranged by size:
4330 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
AvaI C'yCGr_G
Cuts at: 1870 1943 4133 1870
Size: 73 2190 4545
Fragments arranged by size:
4545 2190 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2322 2355 2322
Size: 33 6775
BamHI G'GATC_C
Cuts at: 4138 4505 4138
Size: 367 6441
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3843 4129 5584 618
Size: 325 217 627 2056 286 1455 1842
Fragments arranged by size:
2056 1842 1455 627 325 286 217
BanII G_rGCy'C
Cuts at: 1391 4127 1391
Size: 2736 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3405 3702 928
Size: 971 1506 297 4034
Fragments arranged by size:
4034 1506 971 297
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4999 5033 1903
Size: 34 3062 34 3678
Fragments arranged by size:
3678 3062 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3560 3797 4700 5910 3560
Size: 237 903 1210 4458
Fragments arranged by size:
4458 1210 903 237
BclI T'GATC_A
Cuts at: 1548 2059 1548
Size: 511 6297
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5419 136
Size: 107 122 71 631 605 3747 1525
Fragments arranged by size:
3747 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2224 6803 2224
Size: 4579 2229
BlpI GC'TnA_GC
Cuts at: 4225 4225
Size: 6808
Bme1580I G_kGCm'C
Cuts at: 1790 4869 6025 1790
Size: 3079 1156 2573
Fragments arranged by size:
3079 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6808
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4539 5497 442
Size: 416 53 83 399 24 3122 958 1753
Fragments arranged by size:
3122 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6808
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3051 5468 1239
Size: 1812 2417 2579
Fragments arranged by size:
2579 2417 1812
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2210 4933 5798 6096 2210
Size: 2723 865 298 2922
Fragments arranged by size:
2922 2723 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5471 882
Size: 617 3972 2219
Fragments arranged by size:
3972 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4599 493
Size: 711 3395 2702
Fragments arranged by size:
3395 2702 711
BsaBI GATnn'nnATC
Cuts at: 6802 6802
Size: 6808
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4615 4997 5632 275
Size: 53 83 186 936 3082 382 635 1451
Fragments arranged by size:
3082 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 5241 5639 5913 6060 6471 5241
Size: 398 274 147 411 5578
Fragments arranged by size:
5578 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3648 3678 1178
Size: 30 165 30 2245 30 4308
Fragments arranged by size:
4308 2245 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3764 1186
Size: 2578 4230
BseYI C'CCAG_C
Cuts at: 6011 6507 6011
Size: 496 6312
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3689 1163
Size: 573 1953 4282
Fragments arranged by size:
4282 1953 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4258 5019 6047 1540
Size: 275 186 2257 761 1028 2301
Fragments arranged by size:
2301 2257 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4869 4954 6025 4869
Size: 85 1071 5652
Fragments arranged by size:
5652 1071 85
BsmI GAATG_Cn'
Cuts at: 2891 4319 4412 2891
Size: 1428 93 5287
Fragments arranged by size:
5287 1428 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 4127 4869 4954 6025 1391
Size: 399 2337 742 85 1071 2174
Fragments arranged by size:
2337 2174 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 6471 6471
Size: 6808
BspHI T'CATG_A
Cuts at: 2404 3979 4697 6427 2404
Size: 1575 718 1730 2785
Fragments arranged by size:
2785 1730 1575 718
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4163 844
Size: 3319 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4695 1394
Size: 429 2872 3507
Fragments arranged by size:
3507 2872 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3051 3515 5303 5477 65
Size: 1183 1803 464 1788 174 1396
Fragments arranged by size:
1803 1788 1396 1183 464 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5452 5639 1700
Size: 110 3642 187 2869
Fragments arranged by size:
3642 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6808
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6808
BssSI C'ACGA_G
Cuts at: 4868 5880 4868
Size: 1012 5796
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6808
BstBI TT'CG_AA
Cuts at: 5612 5612
Size: 6808
BstEII G'GTnAC_C
Cuts at: 1184 2294 1184
Size: 1110 5698
BstYI r'GATC_y
Cuts at: 1972 2224 4138 4505 4890 4907 6359 6445 6457
Size: 252 1914 367 385 17 1452 86 12
Cuts at: 6457 6803 1972
Size: 346 1977
Fragments arranged by size:
1977 1914 1452 385 367 346 252 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3230 3230
Size: 6808
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5317
Fragments arranged by size:
5317 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6069
Fragments arranged by size:
6069 683 56
BtsI GCAGTG_nn'
Cuts at: 2812 3845 4324 5118 5138 2812
Size: 1033 479 794 20 4482
Fragments arranged by size:
4482 1033 794 479 20
ClaI AT'CG_AT
Cuts at: 1554 2154 1554
Size: 600 6208
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6773
DraI TTT'AAA
Cuts at: 2662 2704 3201 3889 4112 2662
Size: 42 497 688 223 5358
Fragments arranged by size:
5358 688 497 223 42
DraIII CAC_nnn'GTG
Cuts at: 2099 2099
Size: 6808
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3989 5815 809
Size: 507 2673 1826 1802
Fragments arranged by size:
2673 1826 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4255 5144 6773
Size: 54 1425 50 275 18 2425 889 1629
Cuts at: 6773 8
Size: 43
Fragments arranged by size:
2425 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4255 1537
Size: 275 2443 4090
Fragments arranged by size:
4090 2443 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2634 3741 4738 1353
Size: 1281 1107 997 3423
Fragments arranged by size:
3423 1281 1107 997
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5395 5769 5915 387
Size: 1064 138 3806 374 146 1280
Fragments arranged by size:
3806 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2658 3051 3374 3765 4103 4871 5468
Size: 393 1026 393 323 391 338 768 597
Cuts at: 5468 6255 6523 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1026 787 768 597 393 393 391
338 323 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2927 2927
Size: 6808
EcoRI G'AATT_C
Cuts at: 2483 3482 4117 4495 2483
Size: 999 635 378 4796
Fragments arranged by size:
4796 999 635 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3482 3482
Size: 6808
FspI TGC'GCA
Cuts at: 3584 5314 3584
Size: 1730 5078
FspAI rTGC'GCAy
Cuts at: 3584 3584
Size: 6808
HaeII r_GCGC'y
Cuts at: 1678 5955 1678
Size: 4277 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3142 3174 3617 3649 3740
Size: 32 135 32 1570 32 443 32 91
Cuts at: 3740 3772 5449 5481 5523 5555 1373
Size: 32 1677 32 42 32 2626
Fragments arranged by size:
2626 1677 1570 443 135 91 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2752 4152 4398 669
Size: 988 281 814 1400 246 3079
Fragments arranged by size:
3079 1400 988 814 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6808
HpaI GTT'AAC
Cuts at: 4398 4398
Size: 6808
KpnI G_GTAC'C
Cuts at: 3847 4133 3847
Size: 286 6522
MfeI C'AATT_G
Cuts at: 4407 4407
Size: 6808
MluI A'CGCG_T
Cuts at: 6684 6684
Size: 6808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2047 3489 5922 6106 1305
Size: 742 1442 2433 184 2007
Fragments arranged by size:
2433 2007 1442 742 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4986
Fragments arranged by size:
4986 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2862 4014 4766 5125 5284 518
Size: 648 1696 1152 752 359 159 2042
Fragments arranged by size:
2042 1696 1152 752 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 4224 4901 6049 6294 1606
Size: 2618 677 1148 245 2120
Fragments arranged by size:
2618 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5317
Fragments arranged by size:
5317 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6808
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6808
NotI GC'GGCC_GC
Cuts at: 4255 4255
Size: 6808
NspI r_CATG'y
Cuts at: 1132 1229 3882 4166 5711 1132
Size: 97 2653 284 1545 2229
Fragments arranged by size:
2653 2229 1545 284 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6808
PciI A'CATG_T
Cuts at: 3878 5707 3878
Size: 1829 4979
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3800 3970 1108
Size: 2692 170 3946
Fragments arranged by size:
3946 2692 170
PmeI GTTT'AAAC
Cuts at: 4112 4112
Size: 6808
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4877 4909 6390 6422 4877
Size: 32 1481 32 5263
Fragments arranged by size:
5263 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6808
PsiI TTA'TAA
Cuts at: 4378 4378
Size: 6808
PstI C_TGCA'G
Cuts at: 830 4160 830
Size: 3330 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6808
SalI G'TCGA_C
Cuts at: 1936 4150 1936
Size: 2214 4594
SbfI CC_TGCA'GG
Cuts at: 4160 4160
Size: 6808
ScaI AGT'ACT
Cuts at: 3798 5056 6768 3798
Size: 1258 1712 3838
Fragments arranged by size:
3838 1712 1258
SexAI A'CCwGG_T
Cuts at: 3735 3916 3735
Size: 181 6627
SfcI C'TryA_G
Cuts at: 826 1045 2568 3135 3872 3925 4156 5291 5972
Size: 219 1523 567 737 53 231 1135 681
Cuts at: 5972 6163 826
Size: 191 1471
Fragments arranged by size:
1523 1471 1135 737 681 567 231 219
191 53
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6808
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6808
SmaI CCC'GGG
Cuts at: 1872 4135 1872
Size: 2263 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2189 4912 5813 6075 820
Size: 197 926 246 2723 901 262 1553
Fragments arranged by size:
2723 1553 926 901 262 246 197
SnaBI TAC'GTA
Cuts at: 493 4599 493
Size: 4106 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6808
SphI G_CATG'C
Cuts at: 1132 4166 1132
Size: 3034 3774
SspI AAT'ATT
Cuts at: 5 52 2666 3085 3127 3811 4732 5
Size: 47 2614 419 42 684 921 2081
Fragments arranged by size:
2614 2081 921 684 419 47 42
StuI AGG'CCT
Cuts at: 1517 2875 3012 1517
Size: 1358 137 5313
Fragments arranged by size:
5313 1358 137
StyI C'CwwG_G
Cuts at: 513 1065 2004 4247 513
Size: 552 939 2243 3074
Fragments arranged by size:
3074 2243 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3120 3551 4835 4852 5005 5190 5635
Size: 1255 1540 431 1284 17 153 185 445
Cuts at: 5635 325
Size: 1498
Fragments arranged by size:
1540 1498 1284 1255 445 431 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2283 3796 5054
Size: 274 80 33 51 1443 306 1513 1258
Cuts at: 5054 6766 96
Size: 1712 138
Fragments arranged by size:
1712 1513 1443 1258 306 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2757 2789 6768 6800 2757
Size: 32 3979 32 2765
Fragments arranged by size:
3979 2765 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3156 1316
Size: 1840 4968
XbaI T'CTAG_A
Cuts at: 4144 4144
Size: 6808
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6808
XmaI C'CCGG_G
Cuts at: 1870 4133 1870
Size: 2263 4545
XmnI GAAnn'nnTTC
Cuts at: 4937 4937
Size: 6808
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4616 276
Size: 53 83 186 936 3082 2468
Fragments arranged by size:
3082 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoO109I
EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SfiI SfoI SrfI
SwaI XcmI