Restriction map for
pFN21AE0002
(Circular) (Six-base) MAPSORT of: pFN21AE0002.seq Check: 6998 from: 1 to: 6442
pFN21AE0002 6442 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2104 2213 2104
Size: 109 6333
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4252 278
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3415 3785 1937
Size: 1478 370 4594
Fragments arranged by size:
4594 1478 370
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2766 4505 5889 6157 1632
Size: 1134 1739 1384 268 1917
Fragments arranged by size:
1917 1739 1384 1134 268
AfeI AGC'GCT
Cuts at: 2727 2727
Size: 6442
AflII C'TTAA_G
Cuts at: 820 1017 2729 820
Size: 197 1712 4533
Fragments arranged by size:
4533 1712 197
AflIII A'CryG_T
Cuts at: 1203 5341 6318 1203
Size: 4138 977 1327
Fragments arranged by size:
4138 1327 977
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3179 1184
Size: 1995 4447
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2510 2542 2510
Size: 32 6410
AlwNI CAG_nnn'CTG
Cuts at: 2119 2233 2515 2695 2764 4185 5757 2119
Size: 114 282 180 69 1421 1572 2804
Fragments arranged by size:
2804 1572 1421 282 180 114 69
ApaI G_GGCC'C
Cuts at: 2128 2933 3223 3392 2128
Size: 805 290 169 5178
Fragments arranged by size:
5178 805 290 169
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 2321 3751 3977 4129 6238 1493
Size: 828 1430 226 152 2109 1697
Fragments arranged by size:
2109 1697 1430 828 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 2069 2127 2676 3090 3365 3385 3738
Size: 73 126 58 549 414 275 20 353
Cuts at: 3738 3767 1870
Size: 29 4545
Fragments arranged by size:
4545 549 414 353 275 126 73 58
29 20
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 3058 3155 3283 3763
Size: 325 217 627 947 324 97 128 480
Cuts at: 3763 5218 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 947 627 480 325 324 217
128 97
BanII G_rGCy'C
Cuts at: 1391 2128 2933 3082 3186 3223 3341 3372 3392
Size: 737 805 149 104 37 118 31 20
Cuts at: 3392 3761 1391
Size: 369 4072
Fragments arranged by size:
4072 805 737 369 149 118 104 37
31 20
BbeI G_GCGC'C
Cuts at: 3287 3287
Size: 6442
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5471
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4633 4667 1903
Size: 34 2696 34 3678
Fragments arranged by size:
3678 2696 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4334 5544 4334
Size: 1210 5232
BclI T'GATC_A
Cuts at: 1548 2235 1548
Size: 687 5755
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3577 5053 136
Size: 107 122 71 631 605 1905 1476 1525
Fragments arranged by size:
1905 1525 1476 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2973 6437 2973
Size: 3464 2978
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 2128 2509 2933 3223 3392 3637 4503 5659
Size: 338 381 424 290 169 245 866 1156
Cuts at: 5659 1790
Size: 2573
Fragments arranged by size:
2573 1156 866 424 381 338 290 245
169
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3470 4173 5131
Size: 416 53 83 399 24 2053 703 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
2053 1753 958 703 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2365 2365
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2360 2537 2717 2891 2902 2909 3296 3531
Size: 1121 177 180 174 11 7 387 235
Cuts at: 3531 5102 1239
Size: 1571 2579
Fragments arranged by size:
2579 1571 1121 387 235 180 177 174
11 7
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3356 4567 5432 5730 3356
Size: 1211 865 298 4068
Fragments arranged by size:
4068 1211 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2099 2906 5105 882
Size: 617 600 807 2199 2219
Fragments arranged by size:
2219 2199 807 617 600
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3267 3284 3381 3412
Size: 53 83 186 936 1734 17 97 31
Cuts at: 3412 4249 4631 5266 275
Size: 837 382 635 1451
Fragments arranged by size:
1734 1451 936 837 635 382 186 97
83 53 31 17
BsaWI w'CCGG_w
Cuts at: 3296 4875 5273 5547 5694 6105 3296
Size: 1579 398 274 147 411 3633
Fragments arranged by size:
3633 1579 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2267 2297 2510 2540 3408
Size: 30 165 30 864 30 213 30 868
Cuts at: 3408 3438 3646 3676 1178
Size: 30 208 30 3944
Fragments arranged by size:
3944 868 864 213 208 165 30 30
30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3143 3146 3564 3636 1186
Size: 1957 3 418 72 3992
Fragments arranged by size:
3992 1957 418 72 3
BseYI C'CCAG_C
Cuts at: 2119 2358 2373 2409 3545 3562 5645 6141 2119
Size: 239 15 36 1136 17 2083 496 2420
Fragments arranged by size:
2420 2083 1136 496 239 36 17 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2121 2522 2708 3149 1163
Size: 573 385 401 186 441 4456
Fragments arranged by size:
4456 573 441 401 385 186
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2398 2955 3150 3348 3456 3892
Size: 275 186 397 557 195 198 108 436
Cuts at: 3892 4653 5681 1540
Size: 761 1028 2301
Fragments arranged by size:
2301 1028 761 557 436 397 275 198
195 186 108
BsiHKAI G_wGCw'C
Cuts at: 2178 3186 3372 4503 4588 5659 2178
Size: 1008 186 1131 85 1071 2961
Fragments arranged by size:
2961 1131 1071 1008 186 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
BsmBI CGTCTCn'nnnn_
Cuts at: 2777 2777
Size: 6442
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2128 2178 2509 2933 3082 3186 3223
Size: 399 338 50 331 424 149 104 37
Cuts at: 3223 3341 3372 3392 3637 3761 4503 4588 5659
Size: 118 31 20 245 124 742 85 1071
Cuts at: 5659 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 424 399 338 331 245
149 124 118 104 85 50 37 31
20
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2104 2213 2577 3003 3171 3343 3797 844
Size: 1260 109 364 426 168 172 454 3489
Fragments arranged by size:
3489 1260 454 426 364 172 168 109
BsrBI CCG'CTC
Cuts at: 1394 1823 3136 4329 1394
Size: 429 1313 1193 3507
Fragments arranged by size:
3507 1313 1193 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4937 5111 65
Size: 1183 3689 174 1396
Fragments arranged by size:
3689 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2737 3105 3168 3312 5086 5273 1700
Size: 110 927 368 63 144 1774 187 2869
Fragments arranged by size:
2869 1774 927 368 187 144 110 63
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 2086 3121 1184
Size: 902 1035 4505
Fragments arranged by size:
4505 1035 902
BstYI r'GATC_y
Cuts at: 1972 2973 3772 4139 4524 4541 5993 6079 6091
Size: 1001 799 367 385 17 1452 86 12
Cuts at: 6091 6437 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1001 799 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 3435 513
Size: 552 2370 3520
Fragments arranged by size:
3520 2370 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3637 3958 4752 4772 3637
Size: 321 794 20 5307
Fragments arranged by size:
5307 794 321 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2956 5449 809
Size: 507 1640 2493 1802
Fragments arranged by size:
2493 1802 1640 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2395 2952 3147
Size: 54 1425 50 275 18 565 557 195
Cuts at: 3147 3345 3432 3438 3453 3889 4778 6407 8
Size: 198 87 6 15 436 889 1629 43
Fragments arranged by size:
1629 1425 889 565 557 436 275 198
195 87 54 50 43 18 15 6
EagI C'GGCC_G
Cuts at: 1537 1812 2395 2952 3147 3345 3453 3889 1537
Size: 275 583 557 195 198 108 436 4090
Fragments arranged by size:
4090 583 557 436 275 198 195 108
EarI CTCTTCn'nnn_
Cuts at: 1353 2384 3090 4372 1353
Size: 1031 706 1282 3423
Fragments arranged by size:
3423 1282 1031 706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2510 2633 3010 5029 5403 5549
Size: 1064 138 921 123 377 2019 374 146
Cuts at: 5549 387
Size: 1280
Fragments arranged by size:
2019 1280 1064 921 377 374 146 138
123
EcoICRI GAG'CTC
Cuts at: 3184 3370 3184
Size: 186 6256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2360 2537 2717 2766 2891 2902 2909
Size: 393 728 177 180 49 125 11 7
Cuts at: 2909 3296 3531 4505 5102 5889 6157 1239
Size: 387 235 974 597 787 268 1524
Fragments arranged by size:
1524 974 787 728 597 393 387 268
235 180 177 125 49 11 7
EcoNI CCTnn'n_nnAGG
Cuts at: 2270 2270
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 2124 2692 2800 2929 2930 3389 2124
Size: 568 108 129 1 459 5177
Fragments arranged by size:
5177 568 459 129 108 1
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2729 3287 5589 1678
Size: 1051 558 2302 2531
Fragments arranged by size:
2531 2302 1051 558
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2267 2299 2875 2907 3408
Size: 32 135 32 695 32 576 32 501
Cuts at: 3408 3440 5083 5115 5157 5189 1373
Size: 32 1643 32 42 32 2626
Fragments arranged by size:
2626 1643 695 576 501 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3358 3416 3786 4032 669
Size: 988 281 1420 58 370 246 3079
Fragments arranged by size:
3079 1420 988 370 281 246 58
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6442
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KasI G'GCGC_C
Cuts at: 3283 3283
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 6318 6318
Size: 6442
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2460 5556 5740 1305
Size: 1155 3096 184 2007
Fragments arranged by size:
3096 2007 1155 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2966 4400 4759 4918 518
Size: 648 1800 1434 359 159 2042
Fragments arranged by size:
2042 1800 1434 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2280 2533 3263 3504 3858 4535 5683 5928
Size: 674 253 730 241 354 677 1148 245
Cuts at: 5928 1606
Size: 2120
Fragments arranged by size:
2120 1148 730 677 674 354 253 245
241
NaeI GCC'GGC
Cuts at: 3107 3107
Size: 6442
NarI GG'CG_CC
Cuts at: 3284 3284
Size: 6442
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5890
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NgoMIV G'CCGG_C
Cuts at: 3105 3105
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 3800 5345 1132
Size: 97 2571 1545 2229
Fragments arranged by size:
2571 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 5341 5341
Size: 6442
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6442
PfoI T'CCnGG_A
Cuts at: 2062 2514 2062
Size: 452 5990
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2510 2542 4511 4543 6024 6056 2510
Size: 32 1969 32 1481 32 2896
Fragments arranged by size:
2896 1969 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2800 2800
Size: 6442
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PspOMI G'GGCC_C
Cuts at: 2124 2929 3219 3388 2124
Size: 805 290 169 5178
Fragments arranged by size:
5178 805 290 169
PspXI vC'TCGA_Gb
Cuts at: 3738 3738
Size: 6442
PstI C_TGCA'G
Cuts at: 830 2616 2652 2756 3000 3794 830
Size: 1786 36 104 244 794 3478
Fragments arranged by size:
3478 1786 794 244 104 36
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
SacI G_AGCT'C
Cuts at: 3186 3372 3186
Size: 186 6256
SalI G'TCGA_C
Cuts at: 1936 3414 3784 1936
Size: 1478 370 4594
Fragments arranged by size:
4594 1478 370
SanDI GG'GwC_CC
Cuts at: 2800 2800
Size: 6442
SapI GCTCTTCn'nnn_
Cuts at: 2384 2384
Size: 6442
SbfI CC_TGCA'GG
Cuts at: 3000 3794 3000
Size: 794 5648
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SfcI C'TryA_G
Cuts at: 826 1045 2612 2648 2752 2996 3419 3790 4925
Size: 219 1567 36 104 244 423 371 1135
Cuts at: 4925 5606 5797 826
Size: 681 191 1471
Fragments arranged by size:
1567 1471 1135 681 423 371 244 219
191 104 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 3577 3577
Size: 6442
SfoI GGC'GCC
Cuts at: 3285 3285
Size: 6442
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 2071 2129 3092 3769 1872
Size: 199 58 963 677 4545
Fragments arranged by size:
4545 963 677 199 58
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2729 3371 3738 4546 5447 5709
Size: 197 926 786 642 367 808 901 262
Cuts at: 5709 820
Size: 1553
Fragments arranged by size:
1553 926 901 808 786 642 367 262
197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6442
SphI G_CATG'C
Cuts at: 1132 3800 1132
Size: 2668 3774
SspI AAT'ATT
Cuts at: 5 52 4366 5
Size: 47 4314 2081
Fragments arranged by size:
4314 2081 47
StuI AGG'CCT
Cuts at: 1517 2265 1517
Size: 748 5694
StyI C'CwwG_G
Cuts at: 513 1065 3881 513
Size: 552 2816 3074
Fragments arranged by size:
3074 2816 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2556 3140 3704 4469 4486 4639 4824
Size: 1255 976 584 564 765 17 153 185
Cuts at: 4824 5269 325
Size: 445 1498
Fragments arranged by size:
1498 1255 976 765 584 564 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2199 2547 4688
Size: 274 80 33 51 1443 222 348 2141
Cuts at: 4688 6400 96
Size: 1712 138
Fragments arranged by size:
2141 1712 1443 348 274 222 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2697 2729 3646 3678 6402 6434 2697
Size: 32 917 32 2724 32 2705
Fragments arranged by size:
2724 2705 917 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 3738 1943
Size: 1795 4647
XmaI C'CCGG_G
Cuts at: 1870 2069 2127 3090 3767 1870
Size: 199 58 963 677 4545
Fragments arranged by size:
4545 963 677 199 58
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4250 276
Size: 53 83 186 936 2716 2468
Fragments arranged by size:
2716 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI
BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PsrI PvuII RsrII SacII SexAI
SrfI SwaI XcmI