Restriction map for
pFN21AE0001
(Circular) (Six-base) MAPSORT of: pFN21AE0001.seq Check: 2907 from: 1 to: 6196
pFN21AE0001 6196 bp
With 182 enzymes: SgfI *
August 16, 2010 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2549 2549
Size: 6196
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2586 3539 1937
Size: 649 953 4594
Fragments arranged by size:
4594 953 649
Acc65I G'GTAC_C
Cuts at: 2919 3517 2919
Size: 598 5598
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2799 4259 5643 5911 1632
Size: 1167 1460 1384 268 1917
Fragments arranged by size:
1917 1460 1384 1167 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 2909 5095 6072 1203
Size: 1706 2186 977 1327
Fragments arranged by size:
2186 1706 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6196
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3241 1184
Size: 2057 4139
AlwNI CAG_nnn'CTG
Cuts at: 2344 3939 5511 2344
Size: 1595 1572 3029
Fragments arranged by size:
3029 1595 1572
ApaI G_GGCC'C
Cuts at: 2313 2313
Size: 6196
ApaLI G'TGCA_C
Cuts at: 2479 3335 4253 5409 2479
Size: 856 918 1156 3266
Fragments arranged by size:
3266 1156 918 856
ApoI r'AATT_y
Cuts at: 1493 3505 3731 3883 5992 1493
Size: 2012 226 152 2109 1697
Fragments arranged by size:
2109 2012 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 2139 2431 2467 3456 3521 1870
Size: 73 196 292 36 989 65 4545
Fragments arranged by size:
4545 989 292 196 73 65 36
BamHI G'GATC_C
Cuts at: 2060 3021 3526 3893 2060
Size: 961 505 367 4363
Fragments arranged by size:
4363 961 505 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2919 3517 4972 618
Size: 325 217 627 1132 598 1455 1842
Fragments arranged by size:
1842 1455 1132 627 598 325 217
BanII G_rGCy'C
Cuts at: 1391 2313 3280 3416 3515 1391
Size: 922 967 136 99 4072
Fragments arranged by size:
4072 967 922 136 99
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3407 928
Size: 971 1508 3717
Fragments arranged by size:
3717 1508 971
BbvCI CC'TCA_GC
Cuts at: 3113 3113
Size: 6196
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6196
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2934 4807 136
Size: 107 122 71 631 605 1262 1873 1525
Fragments arranged by size:
1873 1525 1262 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2958 6191 2958
Size: 3233 2963
BlpI GC'TnA_GC
Cuts at: 2108 3192 3613 2108
Size: 1084 421 4691
Fragments arranged by size:
4691 1084 421
Bme1580I G_kGCm'C
Cuts at: 1790 2313 2483 2669 2930 3203 3339 4257 5413
Size: 523 170 186 261 273 136 918 1156
Cuts at: 5413 1790
Size: 2573
Fragments arranged by size:
2573 1156 918 523 273 261 186 170
136
BmgBI CAC'GTC
Cuts at: 1318 3373 3379 1318
Size: 2055 6 4135
Fragments arranged by size:
4135 2055 6
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2365 3283 3927
Size: 416 53 83 399 24 948 918 644
Cuts at: 3927 4885 442
Size: 958 1753
Fragments arranged by size:
1753 958 948 918 644 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2152 2482 2152
Size: 330 5866
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2282 4856 1239
Size: 1043 2574 2579
Fragments arranged by size:
2579 2574 1043
Bpu10I CC'TnA_GC
Cuts at: 3113 3419 3113
Size: 306 5890
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3298 4321 5186 5484 3298
Size: 1023 865 298 4010
Fragments arranged by size:
4010 1023 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2809 4859 882
Size: 617 1310 2050 2219
Fragments arranged by size:
2219 2050 1310 617
BsaAI yAC'GTr
Cuts at: 493 1204 2910 3409 3987 493
Size: 711 1706 499 578 2702
Fragments arranged by size:
2702 1706 711 578 499
BsaBI GATnn'nnATC
Cuts at: 6190 6190
Size: 6196
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4003 4385 5020 275
Size: 53 83 186 936 2470 382 635 1451
Fragments arranged by size:
2470 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3280 3474 4629 5027 5301 5448 5859 3280
Size: 194 1155 398 274 147 411 3617
Fragments arranged by size:
3617 1155 411 398 274 194 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2710 2740 3030 3060 1178
Size: 30 165 30 1307 30 290 30 4314
Fragments arranged by size:
4314 1307 290 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2114 2189 3444 1186
Size: 928 75 1255 3938
Fragments arranged by size:
3938 1255 928 75
BseYI C'CCAG_C
Cuts at: 2672 2929 5399 5895 2672
Size: 257 2470 496 2973
Fragments arranged by size:
2973 2470 496 257
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2174 2234 2408 3125 3182 1163
Size: 573 438 60 174 717 57 4177
Fragments arranged by size:
4177 717 573 438 174 60 57
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3186 3646 4407 5435 1540
Size: 275 186 1185 460 761 1028 2301
Fragments arranged by size:
2301 1185 1028 761 460 275 186
BsiHKAI G_wGCw'C
Cuts at: 2307 2468 2483 3339 4257 4342 5413 2307
Size: 161 15 856 918 85 1071 3090
Fragments arranged by size:
3090 1071 918 856 161 85 15
BsmI GAATG_Cn'
Cuts at: 3707 3800 3707
Size: 93 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2307 2313 2468 2483 2669 2930 3203
Size: 399 517 6 155 15 186 261 273
Cuts at: 3203 3280 3339 3416 3515 4257 4342 5413 1391
Size: 77 59 77 99 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 517 399 273 261 186
155 99 85 77 77 59 15 6
BspEI T'CCGG_A
Cuts at: 3474 5859 3474
Size: 2385 3811
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2352 2549 3484 3551 844
Size: 1508 197 935 67 3489
Fragments arranged by size:
3489 1508 935 197 67
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4691 4865 65
Size: 1183 3443 174 1396
Fragments arranged by size:
3443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2649 4840 5027 1700
Size: 110 839 2191 187 2869
Fragments arranged by size:
2869 2191 839 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6196
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstXI CCAn_nnnn'nTGG
Cuts at: 2323 2323
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 2060 2178 2958 3021 3477 3526 3893 4278
Size: 88 118 780 63 456 49 367 385
Cuts at: 4278 4295 5747 5833 5845 6191 1972
Size: 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 780 456 385 367 346 118
88 86 63 49 17 12
Bsu36I CC'TnA_GG
Cuts at: 2220 2220
Size: 6196
BtgI C'CryG_G
Cuts at: 513 1065 3387 513
Size: 552 2322 3322
Fragments arranged by size:
3322 2322 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 3223 3250 3712 4506 4526 3223
Size: 27 462 794 20 4893
Fragments arranged by size:
4893 794 462 27 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3343 3378 529
Size: 35 2779 35 3347
Fragments arranged by size:
3347 2779 35 35
DraI TTT'AAA
Cuts at: 3500 3500
Size: 6196
DraIII CAC_nnn'GTG
Cuts at: 3103 3103
Size: 6196
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2647 3643 4532
Size: 54 1425 50 275 18 817 996 889
Cuts at: 4532 6161 8
Size: 1629 43
Fragments arranged by size:
1629 1425 996 889 817 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4126 1353
Size: 2773 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2282 2799 4259 4856 5643 5911 1239
Size: 393 650 517 1460 597 787 268 1524
Fragments arranged by size:
1524 1460 787 650 597 517 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2471 3070 2471
Size: 599 5597
EcoRI G'AATT_C
Cuts at: 3505 3883 3505
Size: 378 5818
EcoRV GAT'ATC
Cuts at: 3052 3052
Size: 6196
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3091 3091
Size: 6196
FseI GG_CCGG'CC
Cuts at: 2653 2653
Size: 6196
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 2352 5343 1678
Size: 674 2991 2531
Fragments arranged by size:
2991 2531 674
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3175 3207 4837 4869 4911
Size: 32 135 32 1603 32 1630 32 42
Cuts at: 4911 4943 1373
Size: 32 2626
Fragments arranged by size:
2626 1630 1603 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2848 3540 3786 669
Size: 988 281 910 692 246 3079
Fragments arranged by size:
3079 988 910 692 281 246
HindIII A'AGCT_T
Cuts at: 748 2198 748
Size: 1450 4746
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KpnI G_GTAC'C
Cuts at: 2923 3521 2923
Size: 598 5598
MfeI C'AATT_G
Cuts at: 3795 3795
Size: 6196
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2253 2824 5310 5494 1305
Size: 948 571 2486 184 2007
Fragments arranged by size:
2486 2007 948 571 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4374
Fragments arranged by size:
4374 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2882 4154 4513 4672 518
Size: 648 1716 1272 359 159 2042
Fragments arranged by size:
2042 1716 1272 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2688 3196 3612 4289 5437 5682 1606
Size: 1082 508 416 677 1148 245 2120
Fragments arranged by size:
2120 1148 1082 677 508 416 245
NaeI GCC'GGC
Cuts at: 2651 2651
Size: 6196
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5644
NdeI CA'TA_TG
Cuts at: 387 2996 387
Size: 2609 3587
NgoMIV G'CCGG_C
Cuts at: 2649 2649
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 3554 5099 1132
Size: 97 2325 1545 2229
Fragments arranged by size:
2325 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2160 1348
Size: 812 5384
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6196
PfoI T'CCnGG_A
Cuts at: 3024 3024
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PmlI CAC'GTG
Cuts at: 2910 2910
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3070 3070
Size: 6196
PshAI GACnn'nnGTC
Cuts at: 1613 2861 1613
Size: 1248 4948
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PspOMI G'GGCC_C
Cuts at: 2309 2309
Size: 6196
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2570 2602 2570
Size: 32 6164
PstI C_TGCA'G
Cuts at: 830 2278 2693 3548 830
Size: 1448 415 855 3478
Fragments arranged by size:
3478 1448 855 415
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
PvuII CAG'CTG
Cuts at: 2688 3196 2688
Size: 508 5688
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 4444 6156 4444
Size: 1712 4484
SfcI C'TryA_G
Cuts at: 826 1045 2274 2689 3544 4679 5360 5551 826
Size: 219 1229 415 855 1135 681 191 1471
Fragments arranged by size:
1471 1229 1135 855 681 415 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 2141 3523 1872
Size: 269 1382 4545
Fragments arranged by size:
4545 1382 269
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3313 4300 5201 5463 820
Size: 197 926 1370 987 901 262 1553
Fragments arranged by size:
1553 1370 987 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 2225 3042 3470 1517
Size: 708 817 428 4243
Fragments arranged by size:
4243 817 708 428
StyI C'CwwG_G
Cuts at: 513 1065 2034 2065 3635 513
Size: 552 969 31 1570 3074
Fragments arranged by size:
3074 1570 969 552 31
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2009 2686 4223 4240 4393 4578 5023
Size: 1255 429 677 1537 17 153 185 445
Cuts at: 5023 325
Size: 1498
Fragments arranged by size:
1537 1498 1255 677 445 429 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2238 4442 6154
Size: 274 80 33 51 1443 261 2204 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
2204 1712 1443 274 261 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3030 3062 6156 6188 3030
Size: 32 3094 32 3038
Fragments arranged by size:
3094 3038 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 3532 3532
Size: 6196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2320 2616 3139 2320
Size: 296 523 5377
Fragments arranged by size:
5377 523 296
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 2139 3521 1870
Size: 269 1382 4545
Fragments arranged by size:
4545 1382 269
XmnI GAAnn'nnTTC
Cuts at: 4325 4325
Size: 6196
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I
EcoRI EcoRV FalI FseI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI
BsmBI BstZ17I EcoICRI EcoNI FspAI KasI NarI NruI
NsiI PacI PspXI RsrII SacI SacII SanDI SapI
SexAI SfiI SfoI SrfI SwaI