Restriction map for
pFN21AB9995
(Circular) (Six-base) MAPSORT of: pFN21AB9995.seq Check: 4259 from: 1 to: 6460
pFN21AB9995 6460 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4270 278
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3803 1937
Size: 1866 4594
Acc65I G'GTAC_C
Cuts at: 3781 3781
Size: 6460
AclI AA'CG_TT
Cuts at: 1563 4587 4960 1563
Size: 3024 373 3063
Fragments arranged by size:
3063 3024 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2345 4523 5907 6175 1632
Size: 713 2178 1384 268 1917
Fragments arranged by size:
2178 1917 1384 713 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6263
AflIII A'CryG_T
Cuts at: 1203 2271 2613 2745 5359 6336 1203
Size: 1068 342 132 2614 977 1327
Fragments arranged by size:
2614 1327 1068 977 342 132
AgeI A'CCGG_T
Cuts at: 5291 5291
Size: 6460
AhdI GACnn_n'nnGTC
Cuts at: 5189 5189
Size: 6460
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6460
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3111 3234 3281 1184
Size: 1927 123 47 4363
Fragments arranged by size:
4363 1927 123 47
AlwNI CAG_nnn'CTG
Cuts at: 4203 5775 4203
Size: 1572 4888
ApaLI G'TGCA_C
Cuts at: 4517 5673 4517
Size: 1156 5304
ApoI r'AATT_y
Cuts at: 1493 2467 2478 3769 3995 4147 6256 1493
Size: 974 11 1291 226 152 2109 1697
Fragments arranged by size:
2109 1697 1291 974 226 152 11
AseI AT'TA_AT
Cuts at: 160 1032 5014 160
Size: 872 3982 1606
Fragments arranged by size:
3982 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
AvaI C'yCGr_G
Cuts at: 1870 1943 3785 1870
Size: 73 1842 4545
Fragments arranged by size:
4545 1842 73
BamHI G'GATC_C
Cuts at: 3790 4157 3790
Size: 367 6093
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2091 2732 3781 5236 618
Size: 325 217 627 304 641 1049 1455 1842
Fragments arranged by size:
1842 1455 1049 641 627 325 304 217
BanII G_rGCy'C
Cuts at: 1391 2021 2705 3779 1391
Size: 630 684 1074 4072
Fragments arranged by size:
4072 1074 684 630
BbeI G_GCGC'C
Cuts at: 2736 2736
Size: 6460
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3561 928
Size: 971 1662 3827
Fragments arranged by size:
3827 1662 971
BbvCI CC'TCA_GC
Cuts at: 3501 3501
Size: 6460
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2073 2107 4651 4685 1903
Size: 34 136 34 2544 34 3678
Fragments arranged by size:
3678 2544 136 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4352 5562 4352
Size: 1210 5250
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6460
BfrBI ATG'CAT
Cuts at: 2488 3088 3268 2488
Size: 600 180 5680
Fragments arranged by size:
5680 600 180
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2213 5071 136
Size: 107 122 71 631 605 541 2858 1525
Fragments arranged by size:
2858 1525 631 605 541 122 107 71
BglII A'GATC_T
Cuts at: 3078 6455 3078
Size: 3377 3083
BlpI GC'TnA_GC
Cuts at: 3877 3877
Size: 6460
Bme1580I G_kGCm'C
Cuts at: 1790 4521 5677 1790
Size: 2731 1156 2573
Fragments arranged by size:
2731 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2835 3177 1318
Size: 1517 342 4601
Fragments arranged by size:
4601 1517 342
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4191 5149 442
Size: 416 53 83 399 24 2774 958 1753
Fragments arranged by size:
2774 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6460
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2619 3342 3579 5120 1239
Size: 1380 723 237 1541 2579
Fragments arranged by size:
2579 1541 1380 723 237
Bpu10I CC'TnA_GC
Cuts at: 3501 3501
Size: 6460
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 4585 5450 5748 2843
Size: 1742 865 298 3555
Fragments arranged by size:
3555 1742 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5123 882
Size: 617 3624 2219
Fragments arranged by size:
3624 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4251 493
Size: 711 3047 2702
Fragments arranged by size:
3047 2702 711
BsaBI GATnn'nnATC
Cuts at: 6454 6454
Size: 6460
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2733 3346 4267
Size: 53 83 186 936 624 576 613 921
Cuts at: 4267 4649 5284 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 921 635 624 613 576 382
186 83 53
BsaWI w'CCGG_w
Cuts at: 4893 5291 5565 5712 6123 4893
Size: 398 274 147 411 5230
Fragments arranged by size:
5230 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3648 3678 1178
Size: 30 165 30 2245 30 3960
Fragments arranged by size:
3960 2245 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6460
BseYI C'CCAG_C
Cuts at: 3694 5663 6159 3694
Size: 1969 496 3995
Fragments arranged by size:
3995 1969 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5887
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3910 4671 5699 1540
Size: 275 186 1909 761 1028 2301
Fragments arranged by size:
2301 1909 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3218 4521 4606 5677 3218
Size: 1303 85 1071 4001
Fragments arranged by size:
4001 1303 1071 85
BsmI GAATG_Cn'
Cuts at: 3971 4064 3971
Size: 93 6367
BsmBI CGTCTCn'nnnn_
Cuts at: 2148 2148
Size: 6460
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2021 2705 3218 3779 4521 4606 5677
Size: 399 231 684 513 561 742 85 1071
Cuts at: 5677 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 684 561 513 399 231
85
BspEI T'CCGG_A
Cuts at: 6123 6123
Size: 6460
BspHI T'CATG_A
Cuts at: 4349 6079 4349
Size: 1730 4730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2301 3815 844
Size: 1457 1514 3489
Fragments arranged by size:
3489 1514 1457
BsrBI CCG'CTC
Cuts at: 1394 1823 2178 4347 1394
Size: 429 355 2169 3507
Fragments arranged by size:
3507 2169 429 355
BsrDI GCAATG_nn'
Cuts at: 65 1248 2488 2823 4955 5129 65
Size: 1183 1240 335 2132 174 1396
Fragments arranged by size:
2132 1396 1240 1183 335 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2167 2299 5104 5291 1700
Size: 110 357 132 2805 187 2869
Fragments arranged by size:
2869 2805 357 187 132 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6460
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6460
BssSI C'ACGA_G
Cuts at: 2996 4520 5532 2996
Size: 1524 1012 3924
Fragments arranged by size:
3924 1524 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6460
BstBI TT'CG_AA
Cuts at: 5264 5264
Size: 6460
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6460
BstXI CCAn_nnnn'nTGG
Cuts at: 3616 3616
Size: 6460
BstYI r'GATC_y
Cuts at: 1972 3078 3790 4157 4542 4559 6011 6097 6109
Size: 1106 712 367 385 17 1452 86 12
Cuts at: 6109 6455 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1106 712 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2878 2878
Size: 6460
BtgI C'CryG_G
Cuts at: 513 1065 2004 2195 513
Size: 552 939 191 4778
Fragments arranged by size:
4778 939 552 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2724 487
Size: 683 56 1498 4223
Fragments arranged by size:
4223 1498 683 56
BtsI GCAGTG_nn'
Cuts at: 3218 3976 4770 4790 3218
Size: 758 794 20 4888
Fragments arranged by size:
4888 794 758 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6460
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2451 2486 529
Size: 35 1887 35 4503
Fragments arranged by size:
4503 1887 35 35
DraI TTT'AAA
Cuts at: 3364 3764 3364
Size: 400 6060
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5467 809
Size: 507 4151 1802
Fragments arranged by size:
4151 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2693 3907 4796
Size: 54 1425 50 275 18 863 1214 889
Cuts at: 4796 6425 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1214 889 863 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3907 1537
Size: 275 2095 4090
Fragments arranged by size:
4090 2095 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2817 3231 3558 4390 1353
Size: 1464 414 327 832 3423
Fragments arranged by size:
3423 1464 832 414 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2186 5047 5421 5567 387
Size: 1064 138 597 2861 374 146 1280
Fragments arranged by size:
2861 1280 1064 597 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2345 2619 3342 3579 4523 5120 5907
Size: 393 713 274 723 237 944 597 787
Cuts at: 5907 6175 1239
Size: 268 1524
Fragments arranged by size:
1524 944 787 723 713 597 393 274
268 237
EcoNI CCTnn'n_nnAGG
Cuts at: 3584 3584
Size: 6460
EcoRI G'AATT_C
Cuts at: 3769 4147 3769
Size: 378 6082
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2340 2340
Size: 6460
FspI TGC'GCA
Cuts at: 4966 4966
Size: 6460
HaeII r_GCGC'y
Cuts at: 1678 2736 5607 1678
Size: 1058 2871 2531
Fragments arranged by size:
2871 2531 1058
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2707 2739 3166 3198 3559
Size: 32 135 32 1135 32 427 32 361
Cuts at: 3559 3591 3648 3680 5101 5133 5175 5207 1373
Size: 32 57 32 1421 32 42 32 2626
Fragments arranged by size:
2626 1421 1135 427 361 135 57 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2266 3804 4050 669
Size: 988 281 328 1538 246 3079
Fragments arranged by size:
3079 1538 988 328 281 246
HindIII A'AGCT_T
Cuts at: 748 3538 748
Size: 2790 3670
HpaI GTT'AAC
Cuts at: 4050 4050
Size: 6460
KasI G'GCGC_C
Cuts at: 2732 2732
Size: 6460
KpnI G_GTAC'C
Cuts at: 3785 3785
Size: 6460
MfeI C'AATT_G
Cuts at: 4059 4059
Size: 6460
MluI A'CGCG_T
Cuts at: 2271 6336 2271
Size: 4065 2395
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2022 2551 5574 5758 1305
Size: 717 529 3023 184 2007
Fragments arranged by size:
3023 2007 717 529 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2695 10
Size: 54 1425 343 863 3775
Fragments arranged by size:
3775 1425 863 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3093 3354 3510 4418 4777 4936 518
Size: 648 1927 261 156 908 359 159 2042
Fragments arranged by size:
2042 1927 908 648 359 261 159 156
MspA1I CmG'CkG
Cuts at: 1606 2197 2247 3011 3698 3876 4553 5701 5946
Size: 591 50 764 687 178 677 1148 245
Cuts at: 5946 1606
Size: 2120
Fragments arranged by size:
2120 1148 764 687 677 591 245 178
50
NaeI GCC'GGC
Cuts at: 2169 2301 2169
Size: 132 6328
NarI GG'CG_CC
Cuts at: 2733 2733
Size: 6460
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4969
Fragments arranged by size:
4969 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6460
NgoMIV G'CCGG_C
Cuts at: 2167 2299 2167
Size: 132 6328
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6460
NotI GC'GGCC_GC
Cuts at: 3907 3907
Size: 6460
NsiI A_TGCA'T
Cuts at: 2490 3090 3270 2490
Size: 600 180 5680
Fragments arranged by size:
5680 600 180
NspI r_CATG'y
Cuts at: 1132 1229 2617 2749 3092 3272 3416 3818 5363
Size: 97 1388 132 343 180 144 402 1545
Cuts at: 5363 1132
Size: 2229
Fragments arranged by size:
2229 1545 1388 402 343 180 144 132
97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6460
PciI A'CATG_T
Cuts at: 2613 2745 5359 2613
Size: 132 2614 3714
Fragments arranged by size:
3714 2614 132
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3652 1108
Size: 2544 3916
PmeI GTTT'AAAC
Cuts at: 3764 3764
Size: 6460
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4529 4561 6042 6074 4529
Size: 32 1481 32 4915
Fragments arranged by size:
4915 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6460
PsiI TTA'TAA
Cuts at: 4030 4030
Size: 6460
PstI C_TGCA'G
Cuts at: 830 2246 2875 3812 830
Size: 1416 629 937 3478
Fragments arranged by size:
3478 1416 937 629
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6460
PvuII CAG'CTG
Cuts at: 2247 3011 2247
Size: 764 5696
SacII CC_GC'GG
Cuts at: 2198 2198
Size: 6460
SalI G'TCGA_C
Cuts at: 1936 3802 1936
Size: 1866 4594
SapI GCTCTTCn'nnn_
Cuts at: 2817 2817
Size: 6460
SbfI CC_TGCA'GG
Cuts at: 3812 3812
Size: 6460
ScaI AGT'ACT
Cuts at: 4708 6420 4708
Size: 1712 4748
SfcI C'TryA_G
Cuts at: 826 1045 2242 2871 3808 4943 5624 5815 826
Size: 219 1197 629 937 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 937 681 629 219 191
SfoI GGC'GCC
Cuts at: 2734 2734
Size: 6460
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6460
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6460
SmaI CCC'GGG
Cuts at: 1872 3787 1872
Size: 1915 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 4564 5465 5727 820
Size: 197 926 915 1706 901 262 1553
Fragments arranged by size:
1706 1553 926 915 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4251 493
Size: 3758 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6460
SphI G_CATG'C
Cuts at: 1132 3272 3818 1132
Size: 2140 546 3774
Fragments arranged by size:
3774 2140 546
SspI AAT'ATT
Cuts at: 5 52 4384 5
Size: 47 4332 2081
Fragments arranged by size:
4332 2081 47
StuI AGG'CCT
Cuts at: 1517 3332 1517
Size: 1815 4645
StyI C'CwwG_G
Cuts at: 513 1065 2004 3651 3745 3899 513
Size: 552 939 1647 94 154 3074
Fragments arranged by size:
3074 1647 939 552 154 94
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4487 4504 4657 4842 5287 325
Size: 1255 2907 17 153 185 445 1498
Fragments arranged by size:
2907 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4706 6418 96
Size: 274 80 33 51 1443 2729 1712 138
Fragments arranged by size:
2729 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3006 3038 3133 3165 6420 6452 3006
Size: 32 95 32 3255 32 3014
Fragments arranged by size:
3255 3014 95 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3662 1316
Size: 2346 4114
XbaI T'CTAG_A
Cuts at: 3796 3796
Size: 6460
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6460
XmaI C'CCGG_G
Cuts at: 1870 3785 1870
Size: 1915 4545
XmnI GAAnn'nnTTC
Cuts at: 2378 4589 2378
Size: 2211 4249
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4268 276
Size: 53 83 186 936 2734 2468
Fragments arranged by size:
2734 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SacII SalI SapI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BplI
BsiWI BstZ17I DraIII EcoICRI EcoO109I EcoRV FseI FspAI
NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SanDI SexAI SfiI SrfI SwaI XcmI