Restriction map for
pFN21AB9986
(Circular) (Six-base) MAPSORT of: pFN21AB9986.seq Check: 7877 from: 1 to: 6841
pFN21AB9986 6841 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3109 4651 278
Size: 53 83 186 936 1573 1542 2468
Fragments arranged by size:
2468 1573 1542 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3109 3301 4184 1937
Size: 1172 192 883 4594
Fragments arranged by size:
4594 1172 883 192
Acc65I G'GTAC_C
Cuts at: 4162 4162
Size: 6841
AclI AA'CG_TT
Cuts at: 1563 4968 5341 1563
Size: 3405 373 3063
Fragments arranged by size:
3405 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2363 2972 3129 3554 3578 3733 4904 6288
Size: 731 609 157 425 24 155 1171 1384
Cuts at: 6288 6556 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1171 731 609 425 268 157
155 24
AflII C'TTAA_G
Cuts at: 820 1017 3025 820
Size: 197 2008 4636
Fragments arranged by size:
4636 2008 197
AflIII A'CryG_T
Cuts at: 1203 3326 5740 6717 1203
Size: 2123 2414 977 1327
Fragments arranged by size:
2414 2123 1327 977
AgeI A'CCGG_T
Cuts at: 5672 5672
Size: 6841
AhdI GACnn_n'nnGTC
Cuts at: 5570 5570
Size: 6841
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6841
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3798 1184
Size: 2614 4227
AlwNI CAG_nnn'CTG
Cuts at: 2932 3382 3983 4584 6156 2932
Size: 450 601 601 1572 3617
Fragments arranged by size:
3617 1572 601 601 450
ApaLI G'TGCA_C
Cuts at: 4898 6054 4898
Size: 1156 5685
ApoI r'AATT_y
Cuts at: 1493 2228 2263 2301 2732 3070 3647 4000 4150
Size: 735 35 38 431 338 577 353 150
Cuts at: 4150 4376 4528 6637 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 735 577 431 353 338 226
152 150 38 35
AseI AT'TA_AT
Cuts at: 160 1032 5395 160
Size: 872 4363 1606
Fragments arranged by size:
4363 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
AvaI C'yCGr_G
Cuts at: 1870 1943 4166 1870
Size: 73 2223 4545
Fragments arranged by size:
4545 2223 73
BamHI G'GATC_C
Cuts at: 4171 4538 4171
Size: 367 6474
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4162 5617 618
Size: 325 217 627 2375 1455 1842
Fragments arranged by size:
2375 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4160 1391
Size: 2769 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2591 3251 3581 4109 928
Size: 971 692 660 330 528 3660
Fragments arranged by size:
3660 971 692 660 528 330
BbvCI CC'TCA_GC
Cuts at: 3383 3383
Size: 6841
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5032 5066 1903
Size: 34 3095 34 3678
Fragments arranged by size:
3678 3095 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3300 4733 5943 3300
Size: 1433 1210 4198
Fragments arranged by size:
4198 1433 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6841
BfrBI ATG'CAT
Cuts at: 3365 3365
Size: 6841
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3565 5452 136
Size: 107 122 71 631 605 1893 1887 1525
Fragments arranged by size:
1893 1887 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2067 6836 2067
Size: 4769 2072
BlpI GC'TnA_GC
Cuts at: 2603 4258 2603
Size: 1655 5186
Bme1580I G_kGCm'C
Cuts at: 1790 4902 6058 1790
Size: 3112 1156 2573
Fragments arranged by size:
3112 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2518 1318
Size: 1200 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3496 4572 5530
Size: 416 53 83 399 24 2079 1076 958
Cuts at: 5530 442
Size: 1753
Fragments arranged by size:
2079 1753 1076 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6841
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5501 1239
Size: 4262 2579
Bpu10I CC'TnA_GC
Cuts at: 3383 3383
Size: 6841
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3440 3821 4966 5831 6129 3440
Size: 381 1145 865 298 4152
Fragments arranged by size:
4152 1145 865 381 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5504 882
Size: 617 4005 2219
Fragments arranged by size:
4005 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3130 4632 493
Size: 711 1926 1502 2702
Fragments arranged by size:
2702 1926 1502 711
BsaBI GATnn'nnATC
Cuts at: 6835 6835
Size: 6841
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3106 4648 5030 5665
Size: 53 83 186 936 1573 1542 382 635
Cuts at: 5665 275
Size: 1451
Fragments arranged by size:
1573 1542 1451 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5274 5672 5946 6093 6504 5274
Size: 398 274 147 411 5611
Fragments arranged by size:
5611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3714 3744 4125 4155 1178
Size: 30 165 30 2311 30 381 30 3864
Fragments arranged by size:
3864 2311 381 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2631 3371 3503 1186
Size: 1330 115 740 132 4524
Fragments arranged by size:
4524 1330 740 132 115
BseYI C'CCAG_C
Cuts at: 3018 6044 6540 3018
Size: 3026 496 3319
Fragments arranged by size:
3319 3026 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3795 3896 1163
Size: 573 2059 101 4108
Fragments arranged by size:
4108 2059 573 101
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4291 5052 6080 1540
Size: 275 186 2290 761 1028 2301
Fragments arranged by size:
2301 2290 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2856 4902 4987 6058 2856
Size: 2046 85 1071 3639
Fragments arranged by size:
3639 2046 1071 85
BsmI GAATG_Cn'
Cuts at: 3870 4352 4445 3870
Size: 482 93 6266
Fragments arranged by size:
6266 482 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2856 4160 4902 4987 6058 1391
Size: 399 1066 1304 742 85 1071 2174
Fragments arranged by size:
2174 1304 1071 1066 742 399 85
BspEI T'CCGG_A
Cuts at: 6504 6504
Size: 6841
BspHI T'CATG_A
Cuts at: 2344 2953 4730 6460 2344
Size: 609 1777 1730 2725
Fragments arranged by size:
2725 1777 1730 609
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3225 4196 844
Size: 2381 971 3489
Fragments arranged by size:
3489 2381 971
BsrBI CCG'CTC
Cuts at: 1394 1823 4728 1394
Size: 429 2905 3507
Fragments arranged by size:
3507 2905 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2408 5336 5510 65
Size: 1183 1160 2928 174 1396
Fragments arranged by size:
2928 1396 1183 1160 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5485 5672 1700
Size: 110 3675 187 2869
Fragments arranged by size:
3675 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3323 96
Size: 3227 3614
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6841
BssSI C'ACGA_G
Cuts at: 3941 4901 5913 3941
Size: 960 1012 4869
Fragments arranged by size:
4869 1012 960
BstAPI GCAn_nnn'nTGC
Cuts at: 794 4004 794
Size: 3210 3631
BstBI TT'CG_AA
Cuts at: 2136 5645 2136
Size: 3509 3332
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6841
BstYI r'GATC_y
Cuts at: 1972 2067 4171 4538 4923 4940 6392 6478 6490
Size: 95 2104 367 385 17 1452 86 12
Cuts at: 6490 6836 1972
Size: 346 1977
Fragments arranged by size:
2104 1977 1452 385 367 346 95 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2115 2115
Size: 6841
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5350
Fragments arranged by size:
5350 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6102
Fragments arranged by size:
6102 683 56
BtsI GCAGTG_nn'
Cuts at: 4357 5151 5171 4357
Size: 794 20 6027
Fragments arranged by size:
6027 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6841
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6806
DraI TTT'AAA
Cuts at: 2905 3780 4145 2905
Size: 875 365 5601
Fragments arranged by size:
5601 875 365
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5848 809
Size: 507 4532 1802
Fragments arranged by size:
4532 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4288 5177 6806
Size: 54 1425 50 275 18 2458 889 1629
Cuts at: 6806 8
Size: 43
Fragments arranged by size:
2458 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4288 1537
Size: 275 2476 4090
Fragments arranged by size:
4090 2476 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3280 3285 4771 1353
Size: 1927 5 1486 3423
Fragments arranged by size:
3423 1927 1486 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5428 5802 5948 387
Size: 1064 138 3839 374 146 1280
Fragments arranged by size:
3839 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2363 2972 3129 3554 3578 3733 4904
Size: 393 731 609 157 425 24 155 1171
Cuts at: 4904 5501 6288 6556 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1171 787 731 609 597 425 393
268 157 155 24
EcoO109I rG'GnC_Cy
Cuts at: 2374 2374
Size: 6841
EcoRI G'AATT_C
Cuts at: 2263 4150 4528 2263
Size: 1887 378 4576
Fragments arranged by size:
4576 1887 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2050 2637 2050
Size: 587 6254
FspI TGC'GCA
Cuts at: 5347 5347
Size: 6841
HaeII r_GCGC'y
Cuts at: 1678 5988 1678
Size: 4310 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2477 2509 3350 3382 3712
Size: 32 135 32 905 32 841 32 330
Cuts at: 3712 3744 5482 5514 5556 5588 1373
Size: 32 1738 32 42 32 2626
Fragments arranged by size:
2626 1738 905 841 330 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2720 3302 4185 4431 669
Size: 988 281 782 582 883 246 3079
Fragments arranged by size:
3079 988 883 782 582 281 246
HindIII A'AGCT_T
Cuts at: 748 2126 2674 3141 748
Size: 1378 548 467 4448
Fragments arranged by size:
4448 1378 548 467
HpaI GTT'AAC
Cuts at: 4431 4431
Size: 6841
KpnI G_GTAC'C
Cuts at: 4166 4166
Size: 6841
MfeI C'AATT_G
Cuts at: 4440 4440
Size: 6841
MluI A'CGCG_T
Cuts at: 6717 6717
Size: 6841
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2330 3127 5955 6139 1305
Size: 1025 797 2828 184 2007
Fragments arranged by size:
2828 2007 1025 797 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 5019
Fragments arranged by size:
5019 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2727 3780 4799 5158 5317 518
Size: 648 1561 1053 1019 359 159 2042
Fragments arranged by size:
2042 1561 1053 1019 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3379 3980 4257 4934 6082 6327 1606
Size: 1773 601 277 677 1148 245 2120
Fragments arranged by size:
2120 1773 1148 677 601 277 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5350
Fragments arranged by size:
5350 939 552
NdeI CA'TA_TG
Cuts at: 387 2398 3231 3344 387
Size: 2011 833 113 3884
Fragments arranged by size:
3884 2011 833 113
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6841
NotI GC'GGCC_GC
Cuts at: 4288 4288
Size: 6841
NsiI A_TGCA'T
Cuts at: 3367 3367
Size: 6841
NspI r_CATG'y
Cuts at: 1132 1229 3224 3330 4199 5744 1132
Size: 97 1995 106 869 1545 2229
Fragments arranged by size:
2229 1995 1545 869 106 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6841
PciI A'CATG_T
Cuts at: 3326 5740 3326
Size: 2414 4427
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3739 1108
Size: 2631 4210
PmeI GTTT'AAAC
Cuts at: 4145 4145
Size: 6841
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4910 4942 6423 6455 4910
Size: 32 1481 32 5296
Fragments arranged by size:
5296 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6841
PsiI TTA'TAA
Cuts at: 2978 4085 4411 2978
Size: 1107 326 5408
Fragments arranged by size:
5408 1107 326
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2913 2945 2913
Size: 32 6809
PstI C_TGCA'G
Cuts at: 830 4193 830
Size: 3363 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6841
PvuII CAG'CTG
Cuts at: 3379 3980 3379
Size: 601 6240
SalI G'TCGA_C
Cuts at: 1936 3300 4183 1936
Size: 1364 883 4594
Fragments arranged by size:
4594 1364 883
SbfI CC_TGCA'GG
Cuts at: 4193 4193
Size: 6841
ScaI AGT'ACT
Cuts at: 2691 3657 5089 6801 2691
Size: 966 1432 1712 2731
Fragments arranged by size:
2731 1712 1432 966
SfcI C'TryA_G
Cuts at: 826 1045 4189 5324 6005 6196 826
Size: 219 3144 1135 681 191 1471
Fragments arranged by size:
3144 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6841
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6841
SmaI CCC'GGG
Cuts at: 1872 4168 1872
Size: 2296 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3025 3419 3836 4945 5846 6108
Size: 197 926 1082 394 417 1109 901 262
Cuts at: 6108 820
Size: 1553
Fragments arranged by size:
1553 1109 1082 926 901 417 394 262
197
SnaBI TAC'GTA
Cuts at: 493 4632 493
Size: 4139 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6841
SphI G_CATG'C
Cuts at: 1132 4199 1132
Size: 3067 3774
SspI AAT'ATT
Cuts at: 5 52 4765 5
Size: 47 4713 2081
Fragments arranged by size:
4713 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6841
StyI C'CwwG_G
Cuts at: 513 1065 2004 3463 4280 513
Size: 552 939 1459 817 3074
Fragments arranged by size:
3074 1459 939 817 552
SwaI ATTT'AAAT
Cuts at: 3780 3780
Size: 6841
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2537 4868 4885 5038 5223 5668 325
Size: 1255 957 2331 17 153 185 445 1498
Fragments arranged by size:
2331 1498 1255 957 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2689 3318 3323
Size: 274 80 33 51 1443 712 629 5
Cuts at: 3323 3655 3744 5087 6799 96
Size: 332 89 1343 1712 138
Fragments arranged by size:
1712 1443 1343 712 629 332 274 138
89 80 51 33 5
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3714 3746 6801 6833 3714
Size: 32 3055 32 3722
Fragments arranged by size:
3722 3055 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6841
XbaI T'CTAG_A
Cuts at: 4177 4177
Size: 6841
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6841
XmaI C'CCGG_G
Cuts at: 1870 4166 1870
Size: 2296 4545
XmnI GAAnn'nnTTC
Cuts at: 2511 3239 3428 3705 4970 2511
Size: 728 189 277 1265 4382
Fragments arranged by size:
4382 1265 728 277 189
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3107 4649 276
Size: 53 83 186 936 1573 1542 2468
Fragments arranged by size:
2468 1573 1542 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PmeI PpiI PshAI PsiI
PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI SwaI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI
EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI
SacII SanDI SapI SexAI SfiI SfoI SrfI XcmI