Restriction map for
pFN21AB9985
(Circular) (Six-base) MAPSORT of: pFN21AB9985.seq Check: 907 from: 1 to: 6421
pFN21AB9985 6421 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3308 3308
Size: 6421
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3198 4231 278
Size: 53 83 186 936 1662 1033 2468
Fragments arranged by size:
2468 1662 1033 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2640 3764 1937
Size: 703 1124 4594
Fragments arranged by size:
4594 1124 703
Acc65I G'GTAC_C
Cuts at: 3742 3742
Size: 6421
AclI AA'CG_TT
Cuts at: 1563 4548 4921 1563
Size: 2985 373 3063
Fragments arranged by size:
3063 2985 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2914 4484 5868 6136 1632
Size: 1282 1570 1384 268 1917
Fragments arranged by size:
1917 1570 1384 1282 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6224
AflIII A'CryG_T
Cuts at: 1203 2907 5320 6297 1203
Size: 1704 2413 977 1327
Fragments arranged by size:
2413 1704 1327 977
AgeI A'CCGG_T
Cuts at: 5252 5252
Size: 6421
AhdI GACnn_n'nnGTC
Cuts at: 5150 5150
Size: 6421
AleI CACnn'nnGTG
Cuts at: 1166 2912 1166
Size: 1746 4675
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6421
AlwNI CAG_nnn'CTG
Cuts at: 3062 4164 5736 3062
Size: 1102 1572 3747
Fragments arranged by size:
3747 1572 1102
ApaLI G'TGCA_C
Cuts at: 4478 5634 4478
Size: 1156 5265
ApoI r'AATT_y
Cuts at: 1493 2518 2749 3704 3730 3956 4108 6217 1493
Size: 1025 231 955 26 226 152 2109 1697
Fragments arranged by size:
2109 1697 1025 955 231 226 152 26
AseI AT'TA_AT
Cuts at: 160 1032 4975 160
Size: 872 3943 1606
Fragments arranged by size:
3943 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
AvaI C'yCGr_G
Cuts at: 1870 1943 3664 3746 1870
Size: 73 1721 82 4545
Fragments arranged by size:
4545 1721 82 73
BamHI G'GATC_C
Cuts at: 3682 3751 4118 3682
Size: 69 367 5985
Fragments arranged by size:
5985 367 69
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2444 3208 3742 5197 618
Size: 325 217 627 657 764 534 1455 1842
Fragments arranged by size:
1842 1455 764 657 627 534 325 217
BanII G_rGCy'C
Cuts at: 1391 2279 3740 1391
Size: 888 1461 4072
Fragments arranged by size:
4072 1461 888
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2139 2461 2524 2653 928
Size: 971 240 322 63 129 4696
Fragments arranged by size:
4696 971 322 240 129 63
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4612 4646 1903
Size: 34 2675 34 3678
Fragments arranged by size:
3678 2675 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4313 5523 4313
Size: 1210 5211
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6421
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5032 136
Size: 107 122 71 631 605 3360 1525
Fragments arranged by size:
3360 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2776 6416 2776
Size: 3640 2781
BlpI GC'TnA_GC
Cuts at: 3838 3838
Size: 6421
Bme1580I G_kGCm'C
Cuts at: 1790 4482 5638 1790
Size: 2692 1156 2573
Fragments arranged by size:
2692 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6421
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2177 4152 5110
Size: 416 53 83 399 24 760 1975 958
Cuts at: 5110 442
Size: 1753
Fragments arranged by size:
1975 1753 958 760 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3101 5081 1239
Size: 1862 1980 2579
Fragments arranged by size:
2579 1980 1862
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4546 5411 5709 4546
Size: 865 298 5258
Fragments arranged by size:
5258 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3462 5084 882
Size: 617 1963 1622 2219
Fragments arranged by size:
2219 1963 1622 617
BsaAI yAC'GTr
Cuts at: 493 1204 2695 2910 4212 493
Size: 711 1491 215 1302 2702
Fragments arranged by size:
2702 1491 1302 711 215
BsaBI GATnn'nnATC
Cuts at: 6415 6415
Size: 6421
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3195 4228 4610 5245
Size: 53 83 186 936 1662 1033 382 635
Cuts at: 5245 275
Size: 1451
Fragments arranged by size:
1662 1451 1033 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4854 5252 5526 5673 6084 4854
Size: 398 274 147 411 5191
Fragments arranged by size:
5191 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2220 2250 3705 3735 1178
Size: 30 165 30 817 30 1455 30 3864
Fragments arranged by size:
3864 1455 817 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6421
BseYI C'CCAG_C
Cuts at: 2089 3472 5624 6120 2089
Size: 1383 2152 496 2390
Fragments arranged by size:
2390 2152 1383 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2258 1163
Size: 573 522 5326
Fragments arranged by size:
5326 573 522
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2746 3871 4632 5660 1540
Size: 275 186 745 1125 761 1028 2301
Fragments arranged by size:
2301 1125 1028 761 745 275 186
BsiHKAI G_wGCw'C
Cuts at: 2127 2919 3446 4482 4567 5638 2127
Size: 792 527 1036 85 1071 2910
Fragments arranged by size:
2910 1071 1036 792 527 85
BsmI GAATG_Cn'
Cuts at: 3261 3932 4025 3261
Size: 671 93 5657
Fragments arranged by size:
5657 671 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3361 3361
Size: 6421
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2127 2279 2919 3446 3740 4482 4567
Size: 399 337 152 640 527 294 742 85
Cuts at: 4567 5638 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 640 527 399 337 294
152 85
BspEI T'CCGG_A
Cuts at: 6084 6084
Size: 6421
BspHI T'CATG_A
Cuts at: 2080 2806 4310 6040 2080
Size: 726 1504 1730 2461
Fragments arranged by size:
2461 1730 1504 726
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3070 3257 3308 3776 844
Size: 2226 187 51 468 3489
Fragments arranged by size:
3489 2226 468 187 51
BsrBI CCG'CTC
Cuts at: 1394 1823 4308 1394
Size: 429 2485 3507
Fragments arranged by size:
3507 2485 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2101 4916 5090 65
Size: 1183 853 2815 174 1396
Fragments arranged by size:
2815 1396 1183 853 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2447 3205 5065 5252 1700
Size: 110 637 758 1860 187 2869
Fragments arranged by size:
2869 1860 758 637 187 110
BsrGI T'GTAC_A
Cuts at: 96 3558 96
Size: 3462 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6421
BssSI C'ACGA_G
Cuts at: 2270 3439 4481 5493 2270
Size: 1169 1042 1012 3198
Fragments arranged by size:
3198 1169 1042 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6421
BstBI TT'CG_AA
Cuts at: 5225 5225
Size: 6421
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6421
BstXI CCAn_nnnn'nTGG
Cuts at: 2543 2543
Size: 6421
BstYI r'GATC_y
Cuts at: 1972 2776 3682 3751 4118 4503 4520 5972 6058
Size: 804 906 69 367 385 17 1452 86
Cuts at: 6058 6070 6416 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 906 804 385 367 346 86
69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3212 3267 513
Size: 552 939 1208 55 3667
Fragments arranged by size:
3667 1208 939 552 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2656 487
Size: 683 56 1430 4252
Fragments arranged by size:
4252 1430 683 56
BtsI GCAGTG_nn'
Cuts at: 3937 4731 4751 3937
Size: 794 20 5607
Fragments arranged by size:
5607 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6386
DraI TTT'AAA
Cuts at: 3709 3725 3709
Size: 16 6405
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2416 5428 809
Size: 507 1100 3012 1802
Fragments arranged by size:
3012 1802 1100 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2449 3127 3525
Size: 54 1425 50 275 18 619 678 398
Cuts at: 3525 3868 4757 6386 8
Size: 343 889 1629 43
Fragments arranged by size:
1629 1425 889 678 619 398 343 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3868 1537
Size: 275 2056 4090
Fragments arranged by size:
4090 2056 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4351 1353
Size: 2998 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2964 5008 5382 5528 387
Size: 1064 138 1375 2044 374 146 1280
Fragments arranged by size:
2044 1375 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2914 3101 4484 5081 5868 6136 1239
Size: 393 1282 187 1383 597 787 268 1524
Fragments arranged by size:
1524 1383 1282 787 597 393 268 187
EcoO109I rG'GnC_Cy
Cuts at: 2966 3059 2966
Size: 93 6328
EcoRI G'AATT_C
Cuts at: 3730 4108 3730
Size: 378 6043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2872 2872
Size: 6421
FspI TGC'GCA
Cuts at: 4927 4927
Size: 6421
HaeII r_GCGC'y
Cuts at: 1678 3504 5568 1678
Size: 1826 2064 2531
Fragments arranged by size:
2531 2064 1826
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2022 2054 2794 2826 3461
Size: 32 135 32 450 32 740 32 635
Cuts at: 3461 3493 5062 5094 5136 5168 1373
Size: 32 1569 32 42 32 2626
Fragments arranged by size:
2626 1569 740 635 450 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3175 3765 4011 669
Size: 988 281 1237 590 246 3079
Fragments arranged by size:
3079 1237 988 590 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6421
HpaI GTT'AAC
Cuts at: 3175 4011 3175
Size: 836 5585
KpnI G_GTAC'C
Cuts at: 3746 3746
Size: 6421
MfeI C'AATT_G
Cuts at: 3150 4020 3150
Size: 870 5551
MluI A'CGCG_T
Cuts at: 6297 6297
Size: 6421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2781 5535 5719 1305
Size: 1476 2754 184 2007
Fragments arranged by size:
2754 2007 1476 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3129 3527 10
Size: 54 1425 343 1297 398 2904
Fragments arranged by size:
2904 1425 1297 398 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2630 2910 2912 3185 4379 4738 4897
Size: 648 1464 280 2 273 1194 359 159
Cuts at: 4897 518
Size: 2042
Fragments arranged by size:
2042 1464 1194 648 359 280 273 159
2
MspA1I CmG'CkG
Cuts at: 1606 3837 4514 5662 5907 1606
Size: 2231 677 1148 245 2120
Fragments arranged by size:
2231 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 3212 513
Size: 552 939 1208 3722
Fragments arranged by size:
3722 1208 939 552
NdeI CA'TA_TG
Cuts at: 387 2662 387
Size: 2275 4146
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6421
NotI GC'GGCC_GC
Cuts at: 3868 3868
Size: 6421
NspI r_CATG'y
Cuts at: 1132 1229 3779 5324 1132
Size: 97 2550 1545 2229
Fragments arranged by size:
2550 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3412 1348
Size: 2064 4357
PciI A'CATG_T
Cuts at: 5320 5320
Size: 6421
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2563 1108
Size: 1455 4966
PfoI T'CCnGG_A
Cuts at: 2219 2219
Size: 6421
PmeI GTTT'AAAC
Cuts at: 3725 3725
Size: 6421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4490 4522 6003 6035 4490
Size: 32 1481 32 4876
Fragments arranged by size:
4876 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2966 3059 2966
Size: 93 6328
PshAI GACnn'nnGTC
Cuts at: 1613 3194 1613
Size: 1581 4840
PsiI TTA'TAA
Cuts at: 3991 3991
Size: 6421
PstI C_TGCA'G
Cuts at: 830 2366 2959 3329 3773 830
Size: 1536 593 370 444 3478
Fragments arranged by size:
3478 1536 593 444 370
PvuI CG_AT'CG
Cuts at: 2001 2746 2001
Size: 745 5676
SalI G'TCGA_C
Cuts at: 1936 3763 1936
Size: 1827 4594
SbfI CC_TGCA'GG
Cuts at: 3773 3773
Size: 6421
ScaI AGT'ACT
Cuts at: 4669 6381 4669
Size: 1712 4709
SfcI C'TryA_G
Cuts at: 826 1045 2362 2641 2955 3325 3769 4904 5585
Size: 219 1317 279 314 370 444 1135 681
Cuts at: 5585 5776 826
Size: 191 1471
Fragments arranged by size:
1471 1317 1135 681 444 370 314 279
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6421
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6421
SmaI CCC'GGG
Cuts at: 1872 3666 3748 1872
Size: 1794 82 4545
Fragments arranged by size:
4545 1794 82
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4525 5426 5688 820
Size: 197 926 2582 901 262 1553
Fragments arranged by size:
2582 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4212 493
Size: 3719 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6421
SphI G_CATG'C
Cuts at: 1132 3779 1132
Size: 2647 3774
SspI AAT'ATT
Cuts at: 5 52 4345 5
Size: 47 4293 2081
Fragments arranged by size:
4293 2081 47
StuI AGG'CCT
Cuts at: 1517 3624 1517
Size: 2107 4314
StyI C'CwwG_G
Cuts at: 513 1065 2004 3212 3253 3860 513
Size: 552 939 1208 41 607 3074
Fragments arranged by size:
3074 1208 939 607 552 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3007 3326 4448 4465 4618 4803 5248
Size: 1255 1427 319 1122 17 153 185 445
Cuts at: 5248 325
Size: 1498
Fragments arranged by size:
1498 1427 1255 1122 445 319 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3558 4667 6379
Size: 274 80 33 51 1443 1581 1109 1712
Cuts at: 6379 96
Size: 138
Fragments arranged by size:
1712 1581 1443 1109 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2220 2252 3335 3367 3382 3414 6381 6413 2220
Size: 32 1083 32 15 32 2967 32 2228
Fragments arranged by size:
2967 2228 1083 32 32 32 32 15
Tth111I GACn'n_nGTC
Cuts at: 1316 2970 3239 1316
Size: 1654 269 4498
Fragments arranged by size:
4498 1654 269
XbaI T'CTAG_A
Cuts at: 3757 3757
Size: 6421
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6421
XmaI C'CCGG_G
Cuts at: 1870 3664 3746 1870
Size: 1794 82 4545
Fragments arranged by size:
4545 1794 82
XmnI GAAnn'nnTTC
Cuts at: 4550 4550
Size: 6421
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3196 4229 276
Size: 53 83 186 936 1662 1033 2468
Fragments arranged by size:
2468 1662 1033 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI