Restriction map for
pFN21AB9984
(Circular) (Six-base) MAPSORT of: pFN21AB9984.seq Check: 6286 from: 1 to: 6838
pFN21AB9984 6838 bp
With 182 enzymes: SgfI *
April 15, 2010 09:31 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4648 278
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2307 4181 1937
Size: 370 1874 4594
Fragments arranged by size:
4594 1874 370
Acc65I G'GTAC_C
Cuts at: 4159 4159
Size: 6838
AclI AA'CG_TT
Cuts at: 1563 4965 5338 1563
Size: 3402 373 3063
Fragments arranged by size:
3402 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2420 2617 4901 6285 6553 1632
Size: 788 197 2284 1384 268 1917
Fragments arranged by size:
2284 1917 1384 788 268 197
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6641
AflIII A'CryG_T
Cuts at: 1203 5737 6714 1203
Size: 4534 977 1327
Fragments arranged by size:
4534 1327 977
AgeI A'CCGG_T
Cuts at: 5669 5669
Size: 6838
AhdI GACnn_n'nnGTC
Cuts at: 2460 5567 2460
Size: 3107 3731
AleI CACnn'nnGTG
Cuts at: 1166 3449 1166
Size: 2283 4555
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6838
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3296 3328 3296
Size: 32 6806
AlwNI CAG_nnn'CTG
Cuts at: 3127 3901 4581 6153 3127
Size: 774 680 1572 3812
Fragments arranged by size:
3812 1572 774 680
ApaI G_GGCC'C
Cuts at: 2044 2044
Size: 6838
ApaLI G'TGCA_C
Cuts at: 2089 4895 6051 2089
Size: 2806 1156 2876
Fragments arranged by size:
2876 2806 1156
ApoI r'AATT_y
Cuts at: 1493 2918 3515 3920 4124 4147 4373 4525 6634
Size: 1425 597 405 204 23 226 152 2109
Cuts at: 6634 1493
Size: 1697
Fragments arranged by size:
2109 1697 1425 597 405 226 204 152
23
AseI AT'TA_AT
Cuts at: 160 1032 5392 160
Size: 872 4360 1606
Fragments arranged by size:
4360 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
AvaI C'yCGr_G
Cuts at: 1870 1943 2036 2105 2808 2998 3559 4163 1870
Size: 73 93 69 703 190 561 604 4545
Fragments arranged by size:
4545 703 604 561 190 93 73 69
BamHI G'GATC_C
Cuts at: 4168 4535 4168
Size: 367 6471
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2645 2992 3286 3663 4159
Size: 325 217 627 858 347 294 377 496
Cuts at: 4159 5614 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 858 627 496 377 347 325
294 217
BanII G_rGCy'C
Cuts at: 1391 2044 2496 3509 4157 1391
Size: 653 452 1013 648 4072
Fragments arranged by size:
4072 1013 653 648 452
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2008 928
Size: 971 109 5758
Fragments arranged by size:
5758 971 109
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5029 5063 1903
Size: 34 3092 34 3678
Fragments arranged by size:
3678 3092 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2107 2210 4730 5940 2107
Size: 103 2520 1210 3005
Fragments arranged by size:
3005 2520 1210 103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6838
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2415 2831 3795
Size: 107 122 71 631 605 743 416 964
Cuts at: 3795 5449 136
Size: 1654 1525
Fragments arranged by size:
1654 1525 964 743 631 605 416 122
107 71
BglII A'GATC_T
Cuts at: 2953 3563 6833 2953
Size: 610 3270 2958
Fragments arranged by size:
3270 2958 610
BlpI GC'TnA_GC
Cuts at: 4255 4255
Size: 6838
Bme1580I G_kGCm'C
Cuts at: 1790 2044 2093 2997 3547 3621 4899 6055 1790
Size: 254 49 904 550 74 1278 1156 2573
Fragments arranged by size:
2573 1278 1156 904 550 254 74 49
BmgBI CAC'GTC
Cuts at: 1318 2680 1318
Size: 1362 5476
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3660 4569 5527
Size: 416 53 83 399 24 2243 909 958
Cuts at: 5527 442
Size: 1753
Fragments arranged by size:
2243 1753 958 909 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6838
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2694 3702 5498 1239
Size: 1455 1008 1796 2579
Fragments arranged by size:
2579 1796 1455 1008
Bpu10I CC'TnA_GC
Cuts at: 2490 3753 2490
Size: 1263 5575
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3076 3175 4963 5828 6126 3076
Size: 99 1788 865 298 3788
Fragments arranged by size:
3788 1788 865 298 99
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5501 882
Size: 617 4002 2219
Fragments arranged by size:
4002 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4629 493
Size: 711 3425 2702
Fragments arranged by size:
3425 2702 711
BsaBI GATnn'nnATC
Cuts at: 6832 6832
Size: 6838
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4645 5027 5662 275
Size: 53 83 186 936 3112 382 635 1451
Fragments arranged by size:
3112 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3660 5271 5669 5943 6090 6501 3660
Size: 1611 398 274 147 411 3997
Fragments arranged by size:
3997 1611 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2062 2092 3011 3041 3243
Size: 30 165 30 659 30 919 30 202
Cuts at: 3243 3273 1178
Size: 30 4743
Fragments arranged by size:
4743 919 659 202 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2605 3083 3169 1186
Size: 1419 478 86 4855
Fragments arranged by size:
4855 1419 478 86
BseYI C'CCAG_C
Cuts at: 6041 6537 6041
Size: 496 6342
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2565 1163
Size: 573 829 5436
Fragments arranged by size:
5436 829 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4288 5049 6077 1540
Size: 275 186 2287 761 1028 2301
Fragments arranged by size:
2301 2287 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2093 2702 3580 4097 4899 4984 6055 2093
Size: 609 878 517 802 85 1071 2876
Fragments arranged by size:
2876 1071 878 802 609 517 85
BsmI GAATG_Cn'
Cuts at: 3682 4349 4442 3682
Size: 667 93 6078
Fragments arranged by size:
6078 667 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2658 2670 3678 2658
Size: 12 1008 5818
Fragments arranged by size:
5818 1008 12
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2044 2093 2496 2702 2997 3509 3547
Size: 399 254 49 403 206 295 512 38
Cuts at: 3547 3580 3621 4097 4157 4899 4984 6055 1391
Size: 33 41 476 60 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 512 476 403 399 295
254 206 85 60 49 41 38 33
BspEI T'CCGG_A
Cuts at: 6501 6501
Size: 6838
BspHI T'CATG_A
Cuts at: 4727 6457 4727
Size: 1730 5108
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3182 4193 844
Size: 2338 1011 3489
Fragments arranged by size:
3489 2338 1011
BsrBI CCG'CTC
Cuts at: 1394 1823 4725 1394
Size: 429 2902 3507
Fragments arranged by size:
3507 2902 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2208 3204 3634 5333 5507 65
Size: 1183 960 996 430 1699 174 1396
Fragments arranged by size:
1699 1396 1183 996 960 430 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5482 5669 1700
Size: 110 3672 187 2869
Fragments arranged by size:
3672 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3261 96
Size: 3165 3673
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6838
BssSI C'ACGA_G
Cuts at: 4898 5910 4898
Size: 1012 5826
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3127 3776 794
Size: 2333 649 3856
Fragments arranged by size:
3856 2333 649
BstBI TT'CG_AA
Cuts at: 5642 5642
Size: 6838
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6838
BstXI CCAn_nnnn'nTGG
Cuts at: 2930 3149 3244 2930
Size: 219 95 6524
Fragments arranged by size:
6524 219 95
BstYI r'GATC_y
Cuts at: 1972 2953 3563 3935 4168 4535 4920 4937 6389
Size: 981 610 372 233 367 385 17 1452
Cuts at: 6389 6475 6487 6833 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 981 610 385 372 367 346
233 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 4130 4130
Size: 6838
BtgI C'CryG_G
Cuts at: 513 1065 2346 3450 3480 3840 3858 4024 513
Size: 552 1281 1104 30 360 18 166 3327
Fragments arranged by size:
3327 1281 1104 552 360 166 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6099
Fragments arranged by size:
6099 683 56
BtsI GCAGTG_nn'
Cuts at: 3039 3172 3264 3331 4354 5148 5168 3039
Size: 133 92 67 1023 794 20 4709
Fragments arranged by size:
4709 1023 794 133 92 67 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6838
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6803
DraI TTT'AAA
Cuts at: 4142 4142
Size: 6838
DraIII CAC_nnn'GTG
Cuts at: 2840 4008 2840
Size: 1168 5670
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5845 809
Size: 507 4529 1802
Fragments arranged by size:
4529 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2062 2511 4014
Size: 54 1425 50 275 18 232 449 1503
Cuts at: 4014 4285 5174 6803 8
Size: 271 889 1629 43
Fragments arranged by size:
1629 1503 1425 889 449 275 271 232
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4285 1537
Size: 275 2473 4090
Fragments arranged by size:
4090 2473 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2140 3497 4768 1353
Size: 787 1357 1271 3423
Fragments arranged by size:
3423 1357 1271 787
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2315 2867 5425 5799 5945 387
Size: 1064 138 726 552 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 726 552 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2420 2617 2694 3702 4901 5498 6285
Size: 393 788 197 77 1008 1199 597 787
Cuts at: 6285 6553 1239
Size: 268 1524
Fragments arranged by size:
1524 1199 1008 788 787 597 393 268
197 77
EcoNI CCTnn'n_nnAGG
Cuts at: 3360 3893 4133 3360
Size: 533 240 6065
Fragments arranged by size:
6065 533 240
EcoO109I rG'GnC_Cy
Cuts at: 2040 2041 2713 2812 2040
Size: 1 672 99 6066
Fragments arranged by size:
6066 672 99 1
EcoRI G'AATT_C
Cuts at: 4124 4147 4525 4124
Size: 23 378 6437
Fragments arranged by size:
6437 378 23
EcoRV GAT'ATC
Cuts at: 3368 3368
Size: 6838
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3386 3976 3386
Size: 590 6248
FspI TGC'GCA
Cuts at: 3131 5344 3131
Size: 2213 4625
HaeII r_GCGC'y
Cuts at: 1678 5985 1678
Size: 4307 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2427 2446 2459 2478 2702
Size: 32 135 32 855 19 13 19 224
Cuts at: 2702 2734 3548 3553 3555 3580 3585 3587 5479
Size: 32 814 5 2 25 5 2 1892
Cuts at: 5479 5511 5553 5585 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1892 855 814 224 135 42 32
32 32 32 32 25 19 19 13
5 5 2 2
HincII GTy'rAC
Cuts at: 669 1657 1938 4182 4428 669
Size: 988 281 2244 246 3079
Fragments arranged by size:
3079 2244 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6838
HpaI GTT'AAC
Cuts at: 4428 4428
Size: 6838
KpnI G_GTAC'C
Cuts at: 4163 4163
Size: 6838
MfeI C'AATT_G
Cuts at: 4437 4437
Size: 6838
MluI A'CGCG_T
Cuts at: 6714 6714
Size: 6838
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5952 6136 1305
Size: 4647 184 2007
Fragments arranged by size:
4647 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2513 4016 10
Size: 54 1425 343 681 1503 2832
Fragments arranged by size:
2832 1503 1425 681 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2340 3449 4796 5155 5314 518
Size: 648 1174 1109 1347 359 159 2042
Fragments arranged by size:
2042 1347 1174 1109 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2169 2380 2432 2507 2532 4254 4931 6079
Size: 563 211 52 75 25 1722 677 1148
Cuts at: 6079 6324 1606
Size: 245 2120
Fragments arranged by size:
2120 1722 1148 677 563 245 211 75
52 25
NcoI C'CATG_G
Cuts at: 513 1065 3480 3840 4024 513
Size: 552 2415 360 184 3327
Fragments arranged by size:
3327 2415 552 360 184
NdeI CA'TA_TG
Cuts at: 387 4039 387
Size: 3652 3186
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6838
NotI GC'GGCC_GC
Cuts at: 4285 4285
Size: 6838
NspI r_CATG'y
Cuts at: 1132 1229 4196 5741 1132
Size: 97 2967 1545 2229
Fragments arranged by size:
2967 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3548 1348
Size: 2200 4638
PciI A'CATG_T
Cuts at: 5737 5737
Size: 6838
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6838
PfoI T'CCnGG_A
Cuts at: 4110 4110
Size: 6838
PmeI GTTT'AAAC
Cuts at: 4142 4142
Size: 6838
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4907 4939 6420 6452 4907
Size: 32 1481 32 5293
Fragments arranged by size:
5293 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2713 2812 2713
Size: 99 6739
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6838
PsiI TTA'TAA
Cuts at: 4408 4408
Size: 6838
PspOMI G'GGCC_C
Cuts at: 2040 2040
Size: 6838
PstI C_TGCA'G
Cuts at: 830 3271 3466 4190 830
Size: 2441 195 724 3478
Fragments arranged by size:
3478 2441 724 195
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6838
PvuII CAG'CTG
Cuts at: 2169 2380 2507 2532 2169
Size: 211 127 25 6475
Fragments arranged by size:
6475 211 127 25
SalI G'TCGA_C
Cuts at: 1936 4180 1936
Size: 2244 4594
SanDI GG'GwC_CC
Cuts at: 2812 2812
Size: 6838
SapI GCTCTTCn'nnn_
Cuts at: 3497 3497
Size: 6838
SbfI CC_TGCA'GG
Cuts at: 4190 4190
Size: 6838
ScaI AGT'ACT
Cuts at: 5086 6798 5086
Size: 1712 5126
SfcI C'TryA_G
Cuts at: 826 1045 2292 2448 3267 3462 4186 5321 6002
Size: 219 1247 156 819 195 724 1135 681
Cuts at: 6002 6193 826
Size: 191 1471
Fragments arranged by size:
1471 1247 1135 819 724 681 219 195
191 156
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6838
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6838
SmaI CCC'GGG
Cuts at: 1872 2810 4165 1872
Size: 938 1355 4545
Fragments arranged by size:
4545 1355 938
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3055 3154 4942 5843 6105 820
Size: 197 926 1112 99 1788 901 262 1553
Fragments arranged by size:
1788 1553 1112 926 901 262 197 99
SnaBI TAC'GTA
Cuts at: 493 4629 493
Size: 4136 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6838
SphI G_CATG'C
Cuts at: 1132 4196 1132
Size: 3064 3774
SspI AAT'ATT
Cuts at: 5 52 3527 4049 4762 5
Size: 47 3475 522 713 2081
Fragments arranged by size:
3475 2081 713 522 47
StuI AGG'CCT
Cuts at: 1517 2268 2418 1517
Size: 751 150 5937
Fragments arranged by size:
5937 751 150
StyI C'CwwG_G
Cuts at: 513 1065 3022 3480 3840 4024 4277 513
Size: 552 1957 458 360 184 253 3074
Fragments arranged by size:
3074 1957 552 458 360 253 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2330 4865 4882 5035 5220 5665 325
Size: 1255 750 2535 17 153 185 445 1498
Fragments arranged by size:
2535 1498 1255 750 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3261 5084 6796
Size: 274 80 33 51 1443 1284 1823 1712
Cuts at: 6796 96
Size: 138
Fragments arranged by size:
1823 1712 1443 1284 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2062 2094 2904 2936 3243 3275 6798 6830 2062
Size: 32 810 32 307 32 3523 32 2070
Fragments arranged by size:
3523 2070 810 307 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2052 1316
Size: 736 6102
XbaI T'CTAG_A
Cuts at: 4174 4174
Size: 6838
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2472 2472
Size: 6838
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6838
XmaI C'CCGG_G
Cuts at: 1870 2808 4163 1870
Size: 938 1355 4545
Fragments arranged by size:
4545 1355 938
XmnI GAAnn'nnTTC
Cuts at: 4967 4967
Size: 6838
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4646 276
Size: 53 83 186 936 3112 2468
Fragments arranged by size:
3112 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI
PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstZ17I EcoICRI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI
RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI