Restriction map for
pFN21AB9983
(Circular) (Six-base) MAPSORT of: pFN21AB9983.seq Check: 7037 from: 1 to: 6415
pFN21AB9983 6415 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2369 2369
Size: 6415
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4225 278
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3758 1937
Size: 1821 4594
Acc65I G'GTAC_C
Cuts at: 2849 3736 2849
Size: 887 5528
AclI AA'CG_TT
Cuts at: 1563 4542 4915 1563
Size: 2979 373 3063
Fragments arranged by size:
3063 2979 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2619 4478 5862 6130 1632
Size: 987 1859 1384 268 1917
Fragments arranged by size:
1917 1859 1384 987 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6218
AflIII A'CryG_T
Cuts at: 1203 3476 5314 6291 1203
Size: 2273 1838 977 1327
Fragments arranged by size:
2273 1838 1327 977
AgeI A'CCGG_T
Cuts at: 2789 5246 2789
Size: 2457 3958
AhdI GACnn_n'nnGTC
Cuts at: 5144 5144
Size: 6415
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6415
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6415
AlwNI CAG_nnn'CTG
Cuts at: 2243 2361 4158 5730 2243
Size: 118 1797 1572 2928
Fragments arranged by size:
2928 1797 1572 118
ApaLI G'TGCA_C
Cuts at: 3515 4472 5628 3515
Size: 957 1156 4302
Fragments arranged by size:
4302 1156 957
ApoI r'AATT_y
Cuts at: 1493 2629 3505 3724 3950 4102 6211 1493
Size: 1136 876 219 226 152 2109 1697
Fragments arranged by size:
2109 1697 1136 876 226 219 152
AseI AT'TA_AT
Cuts at: 160 1032 4969 160
Size: 872 3937 1606
Fragments arranged by size:
3937 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
AvaI C'yCGr_G
Cuts at: 1870 1943 2469 3740 1870
Size: 73 526 1271 4545
Fragments arranged by size:
4545 1271 526 73
BamHI G'GATC_C
Cuts at: 3745 4112 3745
Size: 367 6048
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2849 3372 3482 3736 5191
Size: 325 217 627 1062 523 110 254 1455
Cuts at: 5191 618
Size: 1842
Fragments arranged by size:
1842 1455 1062 627 523 325 254 217
110
BanII G_rGCy'C
Cuts at: 1391 3734 1391
Size: 2343 4072
BbeI G_GCGC'C
Cuts at: 3486 3486
Size: 6415
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3003 928
Size: 971 1104 4340
Fragments arranged by size:
4340 1104 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4606 4640 1903
Size: 34 2669 34 3678
Fragments arranged by size:
3678 2669 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3135 4307 5517 3135
Size: 1172 1210 4033
Fragments arranged by size:
4033 1210 1172
BclI T'GATC_A
Cuts at: 1548 2950 1548
Size: 1402 5013
BfrBI ATG'CAT
Cuts at: 3337 3337
Size: 6415
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5026 136
Size: 107 122 71 631 605 3354 1525
Fragments arranged by size:
3354 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6410 6410
Size: 6415
BlpI GC'TnA_GC
Cuts at: 3832 3832
Size: 6415
Bme1580I G_kGCm'C
Cuts at: 1790 2137 3519 4476 5632 1790
Size: 347 1382 957 1156 2573
Fragments arranged by size:
2573 1382 1156 957 347
BmgBI CAC'GTC
Cuts at: 1318 3072 1318
Size: 1754 4661
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4146 5104 442
Size: 416 53 83 399 24 2729 958 1753
Fragments arranged by size:
2729 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 3554 1056
Size: 2498 3917
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2502 2502
Size: 6415
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5075 1239
Size: 3836 2579
Bpu10I CC'TnA_GC
Cuts at: 2680 2680
Size: 6415
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4540 5405 5703 4540
Size: 865 298 5252
Fragments arranged by size:
5252 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2780 3608 5078 882
Size: 617 1281 828 1470 2219
Fragments arranged by size:
2219 1470 1281 828 617
BsaAI yAC'GTr
Cuts at: 493 1204 4206 493
Size: 711 3002 2702
Fragments arranged by size:
3002 2702 711
BsaBI GATnn'nnATC
Cuts at: 2226 3102 6409 2226
Size: 876 3307 2232
Fragments arranged by size:
3307 2232 876
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3483 4222 4604 5239
Size: 53 83 186 936 1950 739 382 635
Cuts at: 5239 275
Size: 1451
Fragments arranged by size:
1950 1451 936 739 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2789 3091 3369 3586 4848 5246 5520 5667 6078
Size: 302 278 217 1262 398 274 147 411
Cuts at: 6078 2789
Size: 3126
Fragments arranged by size:
3126 1262 411 398 302 278 274 217
147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3070 3100 1178
Size: 30 165 30 1667 30 4493
Fragments arranged by size:
4493 1667 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2909 3078 3538 3652 1186
Size: 837 886 169 460 114 3949
Fragments arranged by size:
3949 886 837 460 169 114
BseYI C'CCAG_C
Cuts at: 2916 5618 6114 2916
Size: 2702 496 3217
Fragments arranged by size:
3217 2702 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2524 1163
Size: 573 788 5054
Fragments arranged by size:
5054 788 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3865 4626 5654 1540
Size: 275 186 1864 761 1028 2301
Fragments arranged by size:
2301 1864 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2828 3519 4476 4561 5632 2828
Size: 691 957 85 1071 3611
Fragments arranged by size:
3611 1071 957 691 85
BsmI GAATG_Cn'
Cuts at: 3926 4019 3926
Size: 93 6322
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2137 2828 3519 3734 4476 4561 5632
Size: 399 347 691 691 215 742 85 1071
Cuts at: 5632 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 691 691 399 347 215
85
BspEI T'CCGG_A
Cuts at: 3091 6078 3091
Size: 2987 3428
BspHI T'CATG_A
Cuts at: 2373 4304 6034 2373
Size: 1931 1730 2754
Fragments arranged by size:
2754 1931 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2369 3221 3770 844
Size: 1525 852 549 3489
Fragments arranged by size:
3489 1525 852 549
BsrBI CCG'CTC
Cuts at: 1394 1823 4302 1394
Size: 429 2479 3507
Fragments arranged by size:
3507 2479 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3141 4910 5084 65
Size: 1183 1893 1769 174 1396
Fragments arranged by size:
1893 1769 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2789 5059 5246 1700
Size: 110 979 2270 187 2869
Fragments arranged by size:
2869 2270 979 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6415
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6415
BssSI C'ACGA_G
Cuts at: 4475 5487 4475
Size: 1012 5403
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3256 794
Size: 2462 3953
BstBI TT'CG_AA
Cuts at: 5219 5219
Size: 6415
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6415
BstYI r'GATC_y
Cuts at: 1972 2221 3745 4112 4497 4514 5966 6052 6064
Size: 249 1524 367 385 17 1452 86 12
Cuts at: 6064 6410 1972
Size: 346 1977
Fragments arranged by size:
1977 1524 1452 385 367 346 249 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3521 3521
Size: 6415
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4924
Fragments arranged by size:
4924 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3323 487
Size: 683 56 2097 3579
Fragments arranged by size:
3579 2097 683 56
BtsI GCAGTG_nn'
Cuts at: 2167 2512 3577 3931 4725 4745 2167
Size: 345 1065 354 794 20 3837
Fragments arranged by size:
3837 1065 794 354 345 20
ClaI AT'CG_AT
Cuts at: 1554 3106 1554
Size: 1552 4863
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3460 3495 529
Size: 35 2896 35 3449
Fragments arranged by size:
3449 2896 35 35
DraI TTT'AAA
Cuts at: 3719 3719
Size: 6415
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5422 809
Size: 507 4106 1802
Fragments arranged by size:
4106 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3862 4751 6380
Size: 54 1425 50 275 18 2032 889 1629
Cuts at: 6380 8
Size: 43
Fragments arranged by size:
2032 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3862 1537
Size: 275 2050 4090
Fragments arranged by size:
4090 2050 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2041 2680 3606 4345 1353
Size: 688 639 926 739 3423
Fragments arranged by size:
3423 926 739 688 639
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5002 5376 5522 387
Size: 1064 138 3413 374 146 1280
Fragments arranged by size:
3413 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2619 4478 5075 5862 6130 1239
Size: 393 987 1859 597 787 268 1524
Fragments arranged by size:
1859 1524 987 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2989 2989
Size: 6415
EcoRI G'AATT_C
Cuts at: 2629 3724 4102 2629
Size: 1095 378 4942
Fragments arranged by size:
4942 1095 378
EcoRV GAT'ATC
Cuts at: 2101 2101
Size: 6415
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3008 3635 3008
Size: 627 5788
FspI TGC'GCA
Cuts at: 4921 4921
Size: 6415
HaeII r_GCGC'y
Cuts at: 1678 3486 5562 1678
Size: 1808 2076 2531
Fragments arranged by size:
2531 2076 1808
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2897 2929 5056 5088 5130
Size: 32 135 32 1325 32 2127 32 42
Cuts at: 5130 5162 1373
Size: 32 2626
Fragments arranged by size:
2626 2127 1325 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2671 3759 4005 669
Size: 988 281 733 1088 246 3079
Fragments arranged by size:
3079 1088 988 733 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6415
HpaI GTT'AAC
Cuts at: 4005 4005
Size: 6415
KasI G'GCGC_C
Cuts at: 3482 3482
Size: 6415
KpnI G_GTAC'C
Cuts at: 2853 3740 2853
Size: 887 5528
MfeI C'AATT_G
Cuts at: 4014 4014
Size: 6415
MluI A'CGCG_T
Cuts at: 6291 6291
Size: 6415
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2199 3233 5529 5713 1305
Size: 894 1034 2296 184 2007
Fragments arranged by size:
2296 2007 1034 894 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4593
Fragments arranged by size:
4593 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2094 2570 3286 4373 4732 4891
Size: 648 843 85 476 716 1087 359 159
Cuts at: 4891 518
Size: 2042
Fragments arranged by size:
2042 1087 843 716 648 476 359 159
85
MspA1I CmG'CkG
Cuts at: 1606 3297 3831 4508 5656 5901 1606
Size: 1691 534 677 1148 245 2120
Fragments arranged by size:
2120 1691 1148 677 534 245
NarI GG'CG_CC
Cuts at: 3483 3483
Size: 6415
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4924
Fragments arranged by size:
4924 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6415
NheI G'CTAG_C
Cuts at: 1052 3550 1052
Size: 2498 3917
NotI GC'GGCC_GC
Cuts at: 3862 3862
Size: 6415
NsiI A_TGCA'T
Cuts at: 3339 3339
Size: 6415
NspI r_CATG'y
Cuts at: 1132 1229 3259 3480 3773 5318 1132
Size: 97 2030 221 293 1545 2229
Fragments arranged by size:
2229 2030 1545 293 221 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6415
PciI A'CATG_T
Cuts at: 3476 5314 3476
Size: 1838 4577
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6415
PfoI T'CCnGG_A
Cuts at: 2115 2543 2965 2115
Size: 428 422 5565
Fragments arranged by size:
5565 428 422
PmeI GTTT'AAAC
Cuts at: 3719 3719
Size: 6415
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4484 4516 5997 6029 4484
Size: 32 1481 32 4870
Fragments arranged by size:
4870 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2989 2989
Size: 6415
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6415
PsiI TTA'TAA
Cuts at: 3985 3985
Size: 6415
PstI C_TGCA'G
Cuts at: 830 3767 830
Size: 2937 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6415
SalI G'TCGA_C
Cuts at: 1936 3757 1936
Size: 1821 4594
SbfI CC_TGCA'GG
Cuts at: 3767 3767
Size: 6415
ScaI AGT'ACT
Cuts at: 4663 6375 4663
Size: 1712 4703
SfcI C'TryA_G
Cuts at: 826 1045 3018 3763 4898 5579 5770 826
Size: 219 1973 745 1135 681 191 1471
Fragments arranged by size:
1973 1471 1135 745 681 219 191
SfoI GGC'GCC
Cuts at: 3484 3484
Size: 6415
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6415
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6415
SmaI CCC'GGG
Cuts at: 1872 3742 1872
Size: 1870 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2469 4519 5420 5682 820
Size: 197 926 526 2050 901 262 1553
Fragments arranged by size:
2050 1553 926 901 526 262 197
SnaBI TAC'GTA
Cuts at: 493 4206 493
Size: 3713 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6415
SphI G_CATG'C
Cuts at: 1132 3773 1132
Size: 2641 3774
SspI AAT'ATT
Cuts at: 5 52 2305 4339 5
Size: 47 2253 2034 2081
Fragments arranged by size:
2253 2081 2034 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6415
StyI C'CwwG_G
Cuts at: 513 1065 2004 2690 3854 513
Size: 552 939 686 1164 3074
Fragments arranged by size:
3074 1164 939 686 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 1997 2416 4442 4459 4612 4797 5242
Size: 1255 417 419 2026 17 153 185 445
Cuts at: 5242 325
Size: 1498
Fragments arranged by size:
2026 1498 1255 445 419 417 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3161 4661 6373
Size: 274 80 33 51 1443 1184 1500 1712
Cuts at: 6373 96
Size: 138
Fragments arranged by size:
1712 1500 1443 1184 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3070 3102 6375 6407 3070
Size: 32 3273 32 3078
Fragments arranged by size:
3273 3078 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3174 1316
Size: 1858 4557
XbaI T'CTAG_A
Cuts at: 2425 3751 2425
Size: 1326 5089
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 6415
XhoI C'TCGA_G
Cuts at: 1943 2469 1943
Size: 526 5889
XmaI C'CCGG_G
Cuts at: 1870 3740 1870
Size: 1870 4545
XmnI GAAnn'nnTTC
Cuts at: 3117 3308 4544 3117
Size: 191 1236 4988
Fragments arranged by size:
4988 1236 191
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4223 276
Size: 53 83 186 936 2689 2468
Fragments arranged by size:
2689 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NarI
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI BsiWI
BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI FseI FspAI
NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SrfI SwaI