Restriction map for
pFN21AB9981
(Circular) (Six-base) MAPSORT of: pFN21AB9981.seq Check: 8295 from: 1 to: 6388
pFN21AB9981 6388 bp
With 182 enzymes: SgfI *
April 15, 2010 09:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2602 2602
Size: 6388
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4198 278
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2021 2445 3731 1937
Size: 84 424 1286 4594
Fragments arranged by size:
4594 1286 424 84
Acc65I G'GTAC_C
Cuts at: 3709 3709
Size: 6388
AclI AA'CG_TT
Cuts at: 1563 4515 4888 1563
Size: 2952 373 3063
Fragments arranged by size:
3063 2952 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3182 4451 5835 6103 1632
Size: 1550 1269 1384 268 1917
Fragments arranged by size:
1917 1550 1384 1269 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6191
AflIII A'CryG_T
Cuts at: 1203 5287 6264 1203
Size: 4084 977 1327
Fragments arranged by size:
4084 1327 977
AgeI A'CCGG_T
Cuts at: 5219 5219
Size: 6388
AhdI GACnn_n'nnGTC
Cuts at: 5117 5117
Size: 6388
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6388
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6388
AlwNI CAG_nnn'CTG
Cuts at: 2074 2854 3310 3494 3652 4131 5703 2074
Size: 780 456 184 158 479 1572 2759
Fragments arranged by size:
2759 1572 780 479 456 184 158
ApaI G_GGCC'C
Cuts at: 2646 2646
Size: 6388
ApaLI G'TGCA_C
Cuts at: 4445 5601 4445
Size: 1156 5232
ApoI r'AATT_y
Cuts at: 1493 3697 3923 4075 6184 1493
Size: 2204 226 152 2109 1697
Fragments arranged by size:
2204 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4942 160
Size: 872 3910 1606
Fragments arranged by size:
3910 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
AvaI C'yCGr_G
Cuts at: 1870 1943 2034 2526 2711 3230 3713 1870
Size: 73 91 492 185 519 483 4545
Fragments arranged by size:
4545 519 492 483 185 91 73
BamHI G'GATC_C
Cuts at: 3067 3217 3718 4085 3067
Size: 150 501 367 5370
Fragments arranged by size:
5370 501 367 150
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2142 2311 2473 3247 3709
Size: 325 217 627 355 169 162 774 462
Cuts at: 3709 5164 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 774 627 462 355 325 217
169 162
BanII G_rGCy'C
Cuts at: 1391 2646 2808 3707 1391
Size: 1255 162 899 4072
Fragments arranged by size:
4072 1255 899 162
BbeI G_GCGC'C
Cuts at: 3251 3251
Size: 6388
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5417
BbvCI CC'TCA_GC
Cuts at: 2896 2896
Size: 6388
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4579 4613 1903
Size: 34 2642 34 3678
Fragments arranged by size:
3678 2642 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4280 5490 4280
Size: 1210 5178
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6388
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2169 2991 3356
Size: 107 122 71 631 605 497 822 365
Cuts at: 3356 4999 136
Size: 1643 1525
Fragments arranged by size:
1643 1525 822 631 605 497 365 122
107 71
BglII A'GATC_T
Cuts at: 2611 3298 6383 2611
Size: 687 3085 2616
Fragments arranged by size:
3085 2616 687
BlpI GC'TnA_GC
Cuts at: 3805 3805
Size: 6388
Bme1580I G_kGCm'C
Cuts at: 1790 2041 2181 2457 2478 2646 2747 2970 3420
Size: 251 140 276 21 168 101 223 450
Cuts at: 3420 4449 5605 1790
Size: 1029 1156 2573
Fragments arranged by size:
2573 1156 1029 450 276 251 223 168
140 101 21
BmgBI CAC'GTC
Cuts at: 1318 2019 1318
Size: 701 5687
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2957 3545 4119
Size: 416 53 83 399 24 1540 588 574
Cuts at: 4119 5077 442
Size: 958 1753
Fragments arranged by size:
1753 1540 958 588 574 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6388
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2334 2464 2568 2636 2784 2832 3669 5048
Size: 1095 130 104 68 148 48 837 1379
Cuts at: 5048 1239
Size: 2579
Fragments arranged by size:
2579 1379 1095 837 148 130 104 68
48
Bpu10I CC'TnA_GC
Cuts at: 2896 3180 2896
Size: 284 6104
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4513 5378 5676 4513
Size: 865 298 5225
Fragments arranged by size:
5225 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5051 882
Size: 617 3552 2219
Fragments arranged by size:
3552 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4179 493
Size: 711 2975 2702
Fragments arranged by size:
2975 2702 711
BsaBI GATnn'nnATC
Cuts at: 6382 6382
Size: 6388
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3248 4195 4577 5212
Size: 53 83 186 936 1715 947 382 635
Cuts at: 5212 275
Size: 1451
Fragments arranged by size:
1715 1451 947 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3293 4821 5219 5493 5640 6051 3293
Size: 1528 398 274 147 411 3630
Fragments arranged by size:
3630 1528 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3248 3278 1178
Size: 30 165 30 1845 30 4288
Fragments arranged by size:
4288 1845 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 3155 1186
Size: 1969 4419
BseYI C'CCAG_C
Cuts at: 2025 2212 2225 2245 2250 2320 2412 2776 2847
Size: 187 13 20 5 70 92 364 71
Cuts at: 2847 2951 3097 3323 5591 6087 2025
Size: 104 146 226 2268 496 2326
Fragments arranged by size:
2326 2268 496 364 226 187 146 104
92 71 70 20 13 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3193 3641 1163
Size: 573 1457 448 3910
Fragments arranged by size:
3910 1457 573 448
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3838 4599 5627 1540
Size: 275 186 1837 761 1028 2301
Fragments arranged by size:
2301 1837 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3511 4449 4534 5605 3511
Size: 938 85 1071 4294
Fragments arranged by size:
4294 1071 938 85
BsmI GAATG_Cn'
Cuts at: 3126 3899 3992 3126
Size: 773 93 5522
Fragments arranged by size:
5522 773 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2041 2181 2457 2478 2646 2747 2808
Size: 399 251 140 276 21 168 101 61
Cuts at: 2808 2970 3420 3511 3707 4449 4534 5605 1391
Size: 162 450 91 196 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 450 399 276 251 196
168 162 140 101 91 85 61 21
BspEI T'CCGG_A
Cuts at: 6051 6051
Size: 6388
BspHI T'CATG_A
Cuts at: 4277 6007 4277
Size: 1730 4658
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2602 3515 3743 844
Size: 1758 913 228 3489
Fragments arranged by size:
3489 1758 913 228
BsrBI CCG'CTC
Cuts at: 1394 1823 4275 1394
Size: 429 2452 3507
Fragments arranged by size:
3507 2452 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2010 2293 3335 3545 4883 5057 65
Size: 1183 762 283 1042 210 1338 174 1396
Fragments arranged by size:
1396 1338 1183 1042 762 283 210 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3006 3244 3480 5032 5219 1700
Size: 110 1196 238 236 1552 187 2869
Fragments arranged by size:
2869 1552 1196 238 236 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6388
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6388
BssSI C'ACGA_G
Cuts at: 4448 5460 4448
Size: 1012 5376
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3494 3652 794
Size: 2700 158 3530
Fragments arranged by size:
3530 2700 158
BstBI TT'CG_AA
Cuts at: 5192 5192
Size: 6388
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6388
BstXI CCAn_nnnn'nTGG
Cuts at: 3562 3562
Size: 6388
BstYI r'GATC_y
Cuts at: 1972 2611 2757 3067 3185 3217 3298 3718 4085
Size: 639 146 310 118 32 81 420 367
Cuts at: 4085 4470 4487 5939 6025 6037 6383 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 639 420 385 367 346 310
146 118 86 81 32 17 12
Bsu36I CC'TnA_GG
Cuts at: 2341 2943 2341
Size: 602 5786
BtgI C'CryG_G
Cuts at: 513 1065 2004 3363 513
Size: 552 939 1359 3538
Fragments arranged by size:
3538 1359 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3434 487
Size: 683 56 2208 3441
Fragments arranged by size:
3441 2208 683 56
BtsI GCAGTG_nn'
Cuts at: 3629 3904 4698 4718 3629
Size: 275 794 20 5299
Fragments arranged by size:
5299 794 275 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6388
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6353
DraI TTT'AAA
Cuts at: 3692 3692
Size: 6388
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5395 809
Size: 507 4079 1802
Fragments arranged by size:
4079 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2161 2856
Size: 54 1425 50 275 18 177 154 695
Cuts at: 2856 3135 3835 4724 6353 8
Size: 279 700 889 1629 43
Fragments arranged by size:
1629 1425 889 700 695 279 275 177
154 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3835 1537
Size: 275 2023 4090
Fragments arranged by size:
4090 2023 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4318 1353
Size: 2965 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2072 4975 5349 5495 387
Size: 1064 138 483 2903 374 146 1280
Fragments arranged by size:
2903 1280 1064 483 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2334 2464 2568 2636 2784 2832 3182
Size: 393 702 130 104 68 148 48 350
Cuts at: 3182 3669 4451 5048 5835 6103 1239
Size: 487 782 597 787 268 1524
Fragments arranged by size:
1524 787 782 702 597 487 393 350
268 148 130 104 68 48
EcoNI CCTnn'n_nnAGG
Cuts at: 2979 2979
Size: 6388
EcoO109I rG'GnC_Cy
Cuts at: 2104 2345 2409 2642 2643 2883 2947 3050 3192
Size: 241 64 233 1 240 64 103 142
Cuts at: 3192 3259 3649 2104
Size: 67 390 4843
Fragments arranged by size:
4843 390 241 240 233 142 103 67
64 64 1
EcoRI G'AATT_C
Cuts at: 3697 4075 3697
Size: 378 6010
FspI TGC'GCA
Cuts at: 4894 4894
Size: 6388
HaeII r_GCGC'y
Cuts at: 1678 2124 3251 5535 1678
Size: 446 1127 2284 2531
Fragments arranged by size:
2531 2284 1127 446
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2079 2111 2894 2926 3248
Size: 32 135 32 507 32 783 32 322
Cuts at: 3248 3280 3498 3530 5029 5061 5103 5135 1373
Size: 32 218 32 1499 32 42 32 2626
Fragments arranged by size:
2626 1499 783 507 322 218 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2022 3732 3978 669
Size: 988 281 84 1710 246 3079
Fragments arranged by size:
3079 1710 988 281 246 84
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6388
HpaI GTT'AAC
Cuts at: 3978 3978
Size: 6388
KasI G'GCGC_C
Cuts at: 3247 3247
Size: 6388
KpnI G_GTAC'C
Cuts at: 3713 3713
Size: 6388
MfeI C'AATT_G
Cuts at: 3987 3987
Size: 6388
MluI A'CGCG_T
Cuts at: 6264 6264
Size: 6388
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2360 2691 5502 5686 1305
Size: 1055 331 2811 184 2007
Fragments arranged by size:
2811 2007 1055 331 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2858 10
Size: 54 1425 343 177 849 3540
Fragments arranged by size:
3540 1425 849 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2286 3111 3131 4346 4705 4864 518
Size: 648 1120 825 20 1215 359 159 2042
Fragments arranged by size:
2042 1215 1120 825 648 359 159 20
MspA1I CmG'CkG
Cuts at: 1606 2029 2057 2354 2416 2900 3804 4481 5629
Size: 423 28 297 62 484 904 677 1148
Cuts at: 5629 5874 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 904 677 484 423 297 245
62 28
NaeI GCC'GGC
Cuts at: 3246 3482 3246
Size: 236 6152
NarI GG'CG_CC
Cuts at: 3248 3248
Size: 6388
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4897
Fragments arranged by size:
4897 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6388
NgoMIV G'CCGG_C
Cuts at: 3244 3480 3244
Size: 236 6152
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6388
NotI GC'GGCC_GC
Cuts at: 3835 3835
Size: 6388
NspI r_CATG'y
Cuts at: 1132 1229 3746 5291 1132
Size: 97 2517 1545 2229
Fragments arranged by size:
2517 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3269 3475 3583 1348
Size: 1921 206 108 4153
Fragments arranged by size:
4153 1921 206 108
PciI A'CATG_T
Cuts at: 5287 5287
Size: 6388
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2854 3616 1108
Size: 1746 762 3880
Fragments arranged by size:
3880 1746 762
PfoI T'CCnGG_A
Cuts at: 3000 3000
Size: 6388
PmeI GTTT'AAAC
Cuts at: 3692 3692
Size: 6388
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4457 4489 5970 6002 4457
Size: 32 1481 32 4843
Fragments arranged by size:
4843 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2104 2345 2409 2104
Size: 241 64 6083
Fragments arranged by size:
6083 241 64
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6388
PsiI TTA'TAA
Cuts at: 3958 3958
Size: 6388
PspOMI G'GGCC_C
Cuts at: 2642 2642
Size: 6388
PstI C_TGCA'G
Cuts at: 830 2112 2599 3306 3357 3526 3740 830
Size: 1282 487 707 51 169 214 3478
Fragments arranged by size:
3478 1282 707 487 214 169 51
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6388
PvuII CAG'CTG
Cuts at: 2029 2354 2416 2900 2029
Size: 325 62 484 5517
Fragments arranged by size:
5517 484 325 62
SalI G'TCGA_C
Cuts at: 1936 2020 3730 1936
Size: 84 1710 4594
Fragments arranged by size:
4594 1710 84
SanDI GG'GwC_CC
Cuts at: 2409 2409
Size: 6388
SbfI CC_TGCA'GG
Cuts at: 3357 3740 3357
Size: 383 6005
ScaI AGT'ACT
Cuts at: 4636 6348 4636
Size: 1712 4676
SfcI C'TryA_G
Cuts at: 826 1045 2108 2595 3302 3353 3522 3736 4871
Size: 219 1063 487 707 51 169 214 1135
Cuts at: 4871 5552 5743 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1063 707 681 487 219 214
191 169 51
SfoI GGC'GCC
Cuts at: 3249 3249
Size: 6388
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6388
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6388
SmaI CCC'GGG
Cuts at: 1872 2036 2528 3715 1872
Size: 164 492 1187 4545
Fragments arranged by size:
4545 1187 492 164
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4492 5393 5655 820
Size: 197 926 2549 901 262 1553
Fragments arranged by size:
2549 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4179 493
Size: 3686 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6388
SphI G_CATG'C
Cuts at: 1132 3746 1132
Size: 2614 3774
SrfI GCCC'GGGC
Cuts at: 2036 2036
Size: 6388
SspI AAT'ATT
Cuts at: 5 52 4312 5
Size: 47 4260 2081
Fragments arranged by size:
4260 2081 47
StuI AGG'CCT
Cuts at: 1517 3206 1517
Size: 1689 4699
StyI C'CwwG_G
Cuts at: 513 1065 2004 2436 2817 3054 3827 513
Size: 552 939 432 381 237 773 3074
Fragments arranged by size:
3074 939 773 552 432 381 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2233 4415 4432 4585 4770 5215 325
Size: 1255 653 2182 17 153 185 445 1498
Fragments arranged by size:
2182 1498 1255 653 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3312 4634 6346
Size: 274 80 33 51 1443 1335 1322 1712
Cuts at: 6346 96
Size: 138
Fragments arranged by size:
1712 1443 1335 1322 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2126 2158 3202 3234 6348 6380 2126
Size: 32 1044 32 3114 32 2134
Fragments arranged by size:
3114 2134 1044 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3152 1316
Size: 1836 4552
XbaI T'CTAG_A
Cuts at: 3724 3724
Size: 6388
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2434 2616 3613 2434
Size: 182 997 5209
Fragments arranged by size:
5209 997 182
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6388
XmaI C'CCGG_G
Cuts at: 1870 2034 2526 3713 1870
Size: 164 492 1187 4545
Fragments arranged by size:
4545 1187 492 164
XmnI GAAnn'nnTTC
Cuts at: 4517 4517
Size: 6388
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4196 276
Size: 53 83 186 936 2662 2468
Fragments arranged by size:
2662 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI
NruI NsiI PacI PmlI PspXI PsrI RsrII SacI
SacII SapI SexAI SfiI SwaI