Restriction map for
pFN21AB9977
(Circular) (Six-base) MAPSORT of: pFN21AB9977.seq Check: 4286 from: 1 to: 6376
pFN21AB9977 6376 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2069 2069
Size: 6376
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4186 278
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2118 3719 1937
Size: 181 1601 4594
Fragments arranged by size:
4594 1601 181
Acc65I G'GTAC_C
Cuts at: 3697 3697
Size: 6376
AclI AA'CG_TT
Cuts at: 1563 2892 4503 4876 1563
Size: 1329 1611 373 3063
Fragments arranged by size:
3063 1611 1329 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2436 2508 2982 4439 5823 6091 1632
Size: 804 72 474 1457 1384 268 1917
Fragments arranged by size:
1917 1457 1384 804 474 268 72
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6179
AflIII A'CryG_T
Cuts at: 1203 5275 6252 1203
Size: 4072 977 1327
Fragments arranged by size:
4072 1327 977
AgeI A'CCGG_T
Cuts at: 5207 5207
Size: 6376
AhdI GACnn_n'nnGTC
Cuts at: 5105 5105
Size: 6376
AleI CACnn'nnGTG
Cuts at: 1166 3083 1166
Size: 1917 4459
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6376
AlwNI CAG_nnn'CTG
Cuts at: 4119 5691 4119
Size: 1572 4804
ApaLI G'TGCA_C
Cuts at: 4433 5589 4433
Size: 1156 5220
ApoI r'AATT_y
Cuts at: 1493 2568 2907 3312 3330 3685 3911 4063 6172
Size: 1075 339 405 18 355 226 152 2109
Cuts at: 6172 1493
Size: 1697
Fragments arranged by size:
2109 1697 1075 405 355 339 226 152
18
AseI AT'TA_AT
Cuts at: 160 1032 4930 160
Size: 872 3898 1606
Fragments arranged by size:
3898 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
AvaI C'yCGr_G
Cuts at: 1870 1943 3701 1870
Size: 73 1758 4545
Fragments arranged by size:
4545 1758 73
BamHI G'GATC_C
Cuts at: 3706 4073 3706
Size: 367 6009
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2053 3697 5152 618
Size: 325 217 627 266 1644 1455 1842
Fragments arranged by size:
1842 1644 1455 627 325 266 217
BanII G_rGCy'C
Cuts at: 1391 3695 1391
Size: 2304 4072
BbeI G_GCGC'C
Cuts at: 2057 2057
Size: 6376
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3373 3397 928
Size: 971 1474 24 3907
Fragments arranged by size:
3907 1474 971 24
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2020 2054 4567 4601 1903
Size: 34 83 34 2513 34 3678
Fragments arranged by size:
3678 2513 83 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4268 5478 4268
Size: 1210 5166
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6376
BfrBI ATG'CAT
Cuts at: 3393 3393
Size: 6376
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4987 136
Size: 107 122 71 631 605 3315 1525
Fragments arranged by size:
3315 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6371 6371
Size: 6376
BlpI GC'TnA_GC
Cuts at: 3793 3793
Size: 6376
Bme1580I G_kGCm'C
Cuts at: 1790 4437 5593 1790
Size: 2647 1156 2573
Fragments arranged by size:
2647 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6376
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2217 2866 4107
Size: 416 53 83 399 24 800 649 1241
Cuts at: 4107 5065 442
Size: 958 1753
Fragments arranged by size:
1753 1241 958 800 649 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6376
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2374 3466 2374
Size: 1092 5284
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2379 2991 5036 1239
Size: 1140 612 2045 2579
Fragments arranged by size:
2579 2045 1140 612
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2264 3406 4501 5366 5664 2264
Size: 1142 1095 865 298 2976
Fragments arranged by size:
2976 1142 1095 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5039 882
Size: 617 3540 2219
Fragments arranged by size:
3540 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4167 493
Size: 711 2963 2702
Fragments arranged by size:
2963 2702 711
BsaBI GATnn'nnATC
Cuts at: 2438 3570 6370 2438
Size: 1132 2800 2444
Fragments arranged by size:
2800 2444 1132
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2054 4183 4565 5200
Size: 53 83 186 936 521 2129 382 635
Cuts at: 5200 275
Size: 1451
Fragments arranged by size:
2129 1451 936 635 521 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4809 5207 5481 5628 6039 4809
Size: 398 274 147 411 5146
Fragments arranged by size:
5146 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6151
Fragments arranged by size:
6151 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2091 1186
Size: 905 5471
BseYI C'CCAG_C
Cuts at: 5579 6075 5579
Size: 496 5880
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5803
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3826 4587 5615 1540
Size: 275 186 1825 761 1028 2301
Fragments arranged by size:
2301 1825 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2043 3061 3229 4437 4522 5593 2043
Size: 1018 168 1208 85 1071 2826
Fragments arranged by size:
2826 1208 1071 1018 168 85
BsmI GAATG_Cn'
Cuts at: 3887 3980 3887
Size: 93 6283
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2043 3061 3229 3695 4437 4522 5593
Size: 399 253 1018 168 466 742 85 1071
Cuts at: 5593 1391
Size: 2174
Fragments arranged by size:
2174 1071 1018 742 466 399 253 168
85
BspEI T'CCGG_A
Cuts at: 6039 6039
Size: 6376
BspHI T'CATG_A
Cuts at: 4265 5995 4265
Size: 1730 4646
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2069 3731 844
Size: 1225 1662 3489
Fragments arranged by size:
3489 1662 1225
BsrBI CCG'CTC
Cuts at: 1394 1823 4263 1394
Size: 429 2440 3507
Fragments arranged by size:
3507 2440 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4871 5045 65
Size: 1183 3623 174 1396
Fragments arranged by size:
3623 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5020 5207 1700
Size: 110 3210 187 2869
Fragments arranged by size:
3210 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6376
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6376
BssSI C'ACGA_G
Cuts at: 4436 5448 4436
Size: 1012 5364
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3347 794
Size: 2553 3823
BstBI TT'CG_AA
Cuts at: 5180 5180
Size: 6376
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6376
BstYI r'GATC_y
Cuts at: 1972 3023 3706 4073 4458 4475 5927 6013 6025
Size: 1051 683 367 385 17 1452 86 12
Cuts at: 6025 6371 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1051 683 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2144 2144
Size: 6376
BtgI C'CryG_G
Cuts at: 513 1065 2049 2057 3234 513
Size: 552 984 8 1177 3655
Fragments arranged by size:
3655 1177 984 552 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2175 487
Size: 683 56 949 4688
Fragments arranged by size:
4688 949 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 3892 4686 4706 3265
Size: 627 794 20 4935
Fragments arranged by size:
4935 794 627 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6376
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2765 2800 529
Size: 35 2201 35 4105
Fragments arranged by size:
4105 2201 35 35
DraI TTT'AAA
Cuts at: 2802 3680 2802
Size: 878 5498
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5383 809
Size: 507 4067 1802
Fragments arranged by size:
4067 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3823 4712 6341
Size: 54 1425 50 275 18 1993 889 1629
Cuts at: 6341 8
Size: 43
Fragments arranged by size:
1993 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3823 1537
Size: 275 2011 4090
Fragments arranged by size:
4090 2011 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2527 2958 4306 1353
Size: 1174 431 1348 3423
Fragments arranged by size:
3423 1348 1174 431
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4963 5337 5483 387
Size: 1064 138 3374 374 146 1280
Fragments arranged by size:
3374 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2379 2436 2508 2982 2991 4439 5036
Size: 393 747 57 72 474 9 1448 597
Cuts at: 5036 5823 6091 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1448 787 747 597 474 393 268
72 57 9
EcoRI G'AATT_C
Cuts at: 3685 4063 3685
Size: 378 5998
FspI TGC'GCA
Cuts at: 4882 4882
Size: 6376
HaeII r_GCGC'y
Cuts at: 1678 2057 5523 1678
Size: 379 3466 2531
Fragments arranged by size:
3466 2531 379
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2377 2409 5017 5049 5091
Size: 32 135 32 805 32 2608 32 42
Cuts at: 5091 5123 1373
Size: 32 2626
Fragments arranged by size:
2626 2608 805 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2119 2356 3720 3966 669
Size: 988 281 181 237 1364 246 3079
Fragments arranged by size:
3079 1364 988 281 246 237 181
HindIII A'AGCT_T
Cuts at: 748 2488 2991 748
Size: 1740 503 4133
Fragments arranged by size:
4133 1740 503
HpaI GTT'AAC
Cuts at: 3966 3966
Size: 6376
KasI G'GCGC_C
Cuts at: 2053 2053
Size: 6376
KpnI G_GTAC'C
Cuts at: 3701 3701
Size: 6376
MfeI C'AATT_G
Cuts at: 3534 3975 3534
Size: 441 5935
MluI A'CGCG_T
Cuts at: 6252 6252
Size: 6376
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3307 5490 5674 1305
Size: 2002 2183 184 2007
Fragments arranged by size:
2183 2007 2002 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4554
Fragments arranged by size:
4554 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3083 4334 4693 4852 518
Size: 648 1917 1251 359 159 2042
Fragments arranged by size:
2042 1917 1251 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3792 4469 5617 5862 1606
Size: 2186 677 1148 245 2120
Fragments arranged by size:
2186 2120 1148 677 245
NarI GG'CG_CC
Cuts at: 2054 2054
Size: 6376
NcoI C'CATG_G
Cuts at: 513 1065 2057 3234 513
Size: 552 992 1177 3655
Fragments arranged by size:
3655 1177 992 552
NdeI CA'TA_TG
Cuts at: 387 2758 387
Size: 2371 4005
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6376
NotI GC'GGCC_GC
Cuts at: 3823 3823
Size: 6376
NsiI A_TGCA'T
Cuts at: 3395 3395
Size: 6376
NspI r_CATG'y
Cuts at: 1132 1229 3734 5279 1132
Size: 97 2505 1545 2229
Fragments arranged by size:
2505 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6376
PciI A'CATG_T
Cuts at: 5275 5275
Size: 6376
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6376
PfoI T'CCnGG_A
Cuts at: 2446 2812 3090 2446
Size: 366 278 5732
Fragments arranged by size:
5732 366 278
PmeI GTTT'AAAC
Cuts at: 3680 3680
Size: 6376
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4445 4477 5958 5990 4445
Size: 32 1481 32 4831
Fragments arranged by size:
4831 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6376
PsiI TTA'TAA
Cuts at: 2574 3946 2574
Size: 1372 5004
PstI C_TGCA'G
Cuts at: 830 3728 830
Size: 2898 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6376
SalI G'TCGA_C
Cuts at: 1936 2117 3718 1936
Size: 181 1601 4594
Fragments arranged by size:
4594 1601 181
SbfI CC_TGCA'GG
Cuts at: 3728 3728
Size: 6376
ScaI AGT'ACT
Cuts at: 4624 6336 4624
Size: 1712 4664
SfcI C'TryA_G
Cuts at: 826 1045 2647 3540 3659 3724 4859 5540 5731
Size: 219 1602 893 119 65 1135 681 191
Cuts at: 5731 826
Size: 1471
Fragments arranged by size:
1602 1471 1135 893 681 219 191 119
65
SfoI GGC'GCC
Cuts at: 2055 2055
Size: 6376
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6376
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6376
SmaI CCC'GGG
Cuts at: 1872 3703 1872
Size: 1831 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2279 3385 4480 5381 5643 820
Size: 197 926 336 1106 1095 901 262 1553
Fragments arranged by size:
1553 1106 1095 926 901 336 262 197
SnaBI TAC'GTA
Cuts at: 493 4167 493
Size: 3674 2702
SpeI A'CTAG_T
Cuts at: 152 2252 152
Size: 2100 4276
SphI G_CATG'C
Cuts at: 1132 3734 1132
Size: 2602 3774
SspI AAT'ATT
Cuts at: 5 52 2248 2551 4300 5
Size: 47 2196 303 1749 2081
Fragments arranged by size:
2196 2081 1749 303 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6376
StyI C'CwwG_G
Cuts at: 513 1065 2057 3234 3815 513
Size: 552 992 1177 581 3074
Fragments arranged by size:
3074 1177 992 581 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2050 2226 4403 4420 4573 4758 5203
Size: 1255 470 176 2177 17 153 185 445
Cuts at: 5203 325
Size: 1498
Fragments arranged by size:
2177 1498 1255 470 445 185 176 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2401 2656 4622
Size: 274 80 33 51 1443 424 255 1966
Cuts at: 4622 6334 96
Size: 1712 138
Fragments arranged by size:
1966 1712 1443 424 274 255 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2626 2658 6336 6368 2626
Size: 32 3678 32 2634
Fragments arranged by size:
3678 2634 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6376
XbaI T'CTAG_A
Cuts at: 3712 3712
Size: 6376
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6376
XmaI C'CCGG_G
Cuts at: 1870 3701 1870
Size: 1831 4545
XmnI GAAnn'nnTTC
Cuts at: 3380 3616 4505 3380
Size: 236 889 5251
Fragments arranged by size:
5251 889 236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4184 276
Size: 53 83 186 936 2650 2468
Fragments arranged by size:
2650 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NarI NcoI NdeI NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbvCI Bpu10I
BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoO109I
EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI