Restriction map for
pFN21AB9975
(Circular) (Six-base) MAPSORT of: pFN21AB9975.seq Check: 3186 from: 1 to: 6298
pFN21AB9975 6298 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3576 3576
Size: 6298
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2214 4108 278
Size: 53 83 186 936 678 1894 2468
Fragments arranged by size:
2468 1894 936 678 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3084 3641 1937
Size: 1147 557 4594
Fragments arranged by size:
4594 1147 557
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2297 2432 4361 5745 6013 1632
Size: 665 135 1929 1384 268 1917
Fragments arranged by size:
1929 1917 1384 665 268 135
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 5197 6174 1203
Size: 3994 977 1327
Fragments arranged by size:
3994 1327 977
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 5027 5027
Size: 6298
AleI CACnn'nnGTG
Cuts at: 1166 2333 2351 1166
Size: 1167 18 5113
Fragments arranged by size:
5113 1167 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6298
AlwNI CAG_nnn'CTG
Cuts at: 2677 3280 4041 5613 2677
Size: 603 761 1572 3362
Fragments arranged by size:
3362 1572 761 603
ApaI G_GGCC'C
Cuts at: 2446 2446
Size: 6298
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 3010 3607 3833 3985 6094 1493
Size: 1517 597 226 152 2109 1697
Fragments arranged by size:
2109 1697 1517 597 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 2989 3551 3623 1870
Size: 73 1046 562 72 4545
Fragments arranged by size:
4545 1046 562 73 72
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2921 3619 5074 618
Size: 325 217 627 1134 698 1455 1842
Fragments arranged by size:
1842 1455 1134 698 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2446 2549 3332 3503 3617 1391
Size: 1055 103 783 171 114 4072
Fragments arranged by size:
4072 1055 783 171 114 103
BbeI G_GCGC'C
Cuts at: 2925 2925
Size: 6298
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2420 928
Size: 971 521 4806
Fragments arranged by size:
4806 971 521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6298
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2450 2459 2507
Size: 107 122 71 631 605 778 9 48
Cuts at: 2507 2936 3170 4909 136
Size: 429 234 1739 1525
Fragments arranged by size:
1739 1525 778 631 605 429 234 122
107 71 48 9
BglII A'GATC_T
Cuts at: 6293 6293
Size: 6298
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 2446 3195 4359 5515 1790
Size: 656 749 1164 1156 2573
Fragments arranged by size:
2573 1164 1156 749 656
BmgBI CAC'GTC
Cuts at: 1318 3094 1318
Size: 1776 4522
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4029 4987 442
Size: 416 53 83 399 24 2612 958 1753
Fragments arranged by size:
2612 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4958 1239
Size: 3719 2579
Bpu10I CC'TnA_GC
Cuts at: 2555 2555
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3398 4423 5288 5586 3398
Size: 1025 865 298 4110
Fragments arranged by size:
4110 1025 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3580 4961 882
Size: 617 2081 1381 2219
Fragments arranged by size:
2219 2081 1381 617
BsaAI yAC'GTr
Cuts at: 493 1204 4089 493
Size: 711 2885 2702
Fragments arranged by size:
2885 2702 711
BsaBI GATnn'nnATC
Cuts at: 2189 2984 6292 2189
Size: 795 3308 2195
Fragments arranged by size:
3308 2195 795
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 2211 2922 3081
Size: 53 83 186 936 636 42 711 159
Cuts at: 3081 3441 4105 4487 5122 275
Size: 360 664 382 635 1451
Fragments arranged by size:
1451 936 711 664 636 635 382 360
186 159 83 53 42
BsaWI w'CCGG_w
Cuts at: 2193 4731 5129 5403 5550 5961 2193
Size: 2538 398 274 147 411 2530
Fragments arranged by size:
2538 2530 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6073
Fragments arranged by size:
6073 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2330 3419 3524 1186
Size: 1144 1089 105 3960
Fragments arranged by size:
3960 1144 1089 105
BseYI C'CCAG_C
Cuts at: 2706 3165 5501 5997 2706
Size: 459 2336 496 3007
Fragments arranged by size:
3007 2336 496 459
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2184 2588 2740 3116 3161 3185 3266
Size: 573 448 404 152 376 45 24 81
Cuts at: 3266 3293 3356 3521 1163
Size: 27 63 165 3940
Fragments arranged by size:
3940 573 448 404 376 165 152 81
63 45 27 24
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2514 3748 4509 5537 1540
Size: 275 186 513 1234 761 1028 2301
Fragments arranged by size:
2301 1234 1028 761 513 275 186
BsiHKAI G_wGCw'C
Cuts at: 2556 4359 4444 5515 2556
Size: 1803 85 1071 3339
Fragments arranged by size:
3339 1803 1071 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2446 2549 2556 3195 3332 3503 3617
Size: 399 656 103 7 639 137 171 114
Cuts at: 3617 4359 4444 5515 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 656 639 399 171 137
114 103 85 7
BspEI T'CCGG_A
Cuts at: 2193 5961 2193
Size: 3768 2530
BspHI T'CATG_A
Cuts at: 2094 4187 5917 2094
Size: 2093 1730 2475
Fragments arranged by size:
2475 2093 1730
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2850 2963 3543 3576 3653 844
Size: 2006 113 580 33 77 3489
Fragments arranged by size:
3489 2006 580 113 77 33
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3439 4793 4967 65
Size: 1183 2191 1354 174 1396
Fragments arranged by size:
2191 1396 1354 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4942 5129 1700
Size: 110 3132 187 2869
Fragments arranged by size:
3132 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 4358 5370 4358
Size: 1012 5286
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3199 794
Size: 2405 3893
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 2257 2353 1184
Size: 1073 96 5129
Fragments arranged by size:
5129 1073 96
BstXI CCAn_nnnn'nTGG
Cuts at: 2332 2332
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 2048 3108 3409 3628 3995 4380 4397 5849
Size: 76 1060 301 219 367 385 17 1452
Cuts at: 5849 5935 5947 6293 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1060 385 367 346 301 219
86 76 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2331 2514 2880 2886 2917 3504
Size: 552 939 327 183 366 6 31 587
Cuts at: 3504 513
Size: 3307
Fragments arranged by size:
3307 939 587 552 366 327 183 31
6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2546 487
Size: 683 56 1320 4239
Fragments arranged by size:
4239 1320 683 56
BtsI GCAGTG_nn'
Cuts at: 3814 4608 4628 3814
Size: 794 20 5484
Fragments arranged by size:
5484 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2015 5305 809
Size: 507 699 3290 1802
Fragments arranged by size:
3290 1802 699 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2371 2511 2535
Size: 54 1425 50 275 18 541 140 24
Cuts at: 2535 2883 2937 3171 3363 3745 4634 6263 8
Size: 348 54 234 192 382 889 1629 43
Fragments arranged by size:
1629 1425 889 541 382 348 275 234
192 140 54 54 50 43 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 2511 3745 1537
Size: 275 699 1234 4090
Fragments arranged by size:
4090 1234 699 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2582 4228 1353
Size: 1229 1646 3423
Fragments arranged by size:
3423 1646 1229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3164 4885 5259 5405 387
Size: 1064 138 1575 1721 374 146 1280
Fragments arranged by size:
1721 1575 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2297 2432 4361 4958 5745 6013 1239
Size: 393 665 135 1929 597 787 268 1524
Fragments arranged by size:
1929 1524 787 665 597 393 268 135
EcoNI CCTnn'n_nnAGG
Cuts at: 3284 3284
Size: 6298
EcoO109I rG'GnC_Cy
Cuts at: 2674 3277 3418 3523 2674
Size: 603 141 105 5449
Fragments arranged by size:
5449 603 141 105
EcoRI G'AATT_C
Cuts at: 3607 3985 3607
Size: 378 5920
FspI TGC'GCA
Cuts at: 2765 4804 2765
Size: 2039 4259
HaeII r_GCGC'y
Cuts at: 1678 2706 2925 5445 1678
Size: 1028 219 2520 2531
Fragments arranged by size:
2531 2520 1028 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2076 2108 4939 4971 5013
Size: 32 135 32 504 32 2831 32 42
Cuts at: 5013 5045 1373
Size: 32 2626
Fragments arranged by size:
2831 2626 504 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3888 669
Size: 988 281 1704 246 3079
Fragments arranged by size:
3079 1704 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6298
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 2921 2921
Size: 6298
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2421 2957 3405 5412 5596 1305
Size: 1116 536 448 2007 184 2007
Fragments arranged by size:
2007 2007 1116 536 448 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2939 3173 10
Size: 54 1425 343 1107 234 3135
Fragments arranged by size:
3135 1425 1107 343 234 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2303 2333 2351 4256 4615 4774 518
Size: 648 1137 30 18 1905 359 159 2042
Fragments arranged by size:
2042 1905 1137 648 359 159 30 18
MspA1I CmG'CkG
Cuts at: 1606 2888 2963 3169 3250 3714 4391 5539 5784
Size: 1282 75 206 81 464 677 1148 245
Cuts at: 5784 1606
Size: 2120
Fragments arranged by size:
2120 1282 1148 677 464 245 206 81
75
NarI GG'CG_CC
Cuts at: 2922 2922
Size: 6298
NcoI C'CATG_G
Cuts at: 513 1065 2004 2331 2880 513
Size: 552 939 327 549 3931
Fragments arranged by size:
3931 939 552 549 327
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 3656 5201 1132
Size: 97 2427 1545 2229
Fragments arranged by size:
2427 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2236 1348
Size: 888 5410
PciI A'CATG_T
Cuts at: 5197 5197
Size: 6298
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2881 1108
Size: 1773 4525
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3418 3418
Size: 6298
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PspOMI G'GGCC_C
Cuts at: 2442 2442
Size: 6298
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2564 2596 2564
Size: 32 6266
PstI C_TGCA'G
Cuts at: 830 2119 2460 2685 3650 830
Size: 1289 341 225 965 3478
Fragments arranged by size:
3478 1289 965 341 225
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 2963 3169 3250 2963
Size: 206 81 6011
Fragments arranged by size:
6011 206 81
RsrII CG'GwC_CG
Cuts at: 2084 2084
Size: 6298
SacII CC_GC'GG
Cuts at: 2889 2889
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SbfI CC_TGCA'GG
Cuts at: 2460 3650 2460
Size: 1190 5108
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SfcI C'TryA_G
Cuts at: 826 1045 2115 2456 2681 3646 4781 5462 5653
Size: 219 1070 341 225 965 1135 681 191
Cuts at: 5653 826
Size: 1471
Fragments arranged by size:
1471 1135 1070 965 681 341 225 219
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2450 2936 3170 2450
Size: 486 234 5578
Fragments arranged by size:
5578 486 234
SfoI GGC'GCC
Cuts at: 2923 2923
Size: 6298
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 2991 3625 1872
Size: 1119 634 4545
Fragments arranged by size:
4545 1119 634
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3413 4402 5303 5565 820
Size: 197 926 1470 989 901 262 1553
Fragments arranged by size:
1553 1470 989 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3656 1132
Size: 2524 3774
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 2294 1517
Size: 777 5521
StyI C'CwwG_G
Cuts at: 513 1065 2004 2331 2493 2880 3285 3737 513
Size: 552 939 327 162 387 405 452 3074
Fragments arranged by size:
3074 939 552 452 405 387 327 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2432 2461 4325 4342 4495 4680 5125
Size: 1255 852 29 1864 17 153 185 445
Cuts at: 5125 325
Size: 1498
Fragments arranged by size:
1864 1498 1255 852 445 185 153 29
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2802 4544 6256
Size: 274 80 33 51 1443 825 1742 1712
Cuts at: 6256 96
Size: 138
Fragments arranged by size:
1742 1712 1443 825 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6258 6290 6258
Size: 32 6266
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6298
XbaI T'CTAG_A
Cuts at: 2177 3634 2177
Size: 1457 4841
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2722 2992 2722
Size: 270 6028
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 2989 3623 1870
Size: 1119 634 4545
Fragments arranged by size:
4545 1119 634
XmnI GAAnn'nnTTC
Cuts at: 2576 3350 4427 2576
Size: 774 1077 4447
Fragments arranged by size:
4447 1077 774
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2212 4106 276
Size: 53 83 186 936 678 1894 2468
Fragments arranged by size:
2468 1894 936 678 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NarI NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII RsrII SacII SalI SbfI ScaI SfcI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI
FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI
PspXI SacI SanDI SapI SexAI SrfI SwaI