Restriction map for
pFN21AB9972
(Circular) (Six-base) MAPSORT of: pFN21AB9972.seq Check: 8135 from: 1 to: 6817
pFN21AB9972 6817 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4627 278
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4160 1937
Size: 2223 4594
Acc65I G'GTAC_C
Cuts at: 4138 4138
Size: 6817
AclI AA'CG_TT
Cuts at: 1563 4944 5317 1563
Size: 3381 373 3063
Fragments arranged by size:
3381 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4880 6264 6532 1632
Size: 3248 1384 268 1917
Fragments arranged by size:
3248 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6620
AflIII A'CryG_T
Cuts at: 1203 4114 5716 6693 1203
Size: 2911 1602 977 1327
Fragments arranged by size:
2911 1602 1327 977
AgeI A'CCGG_T
Cuts at: 5648 5648
Size: 6817
AhdI GACnn_n'nnGTC
Cuts at: 5546 5546
Size: 6817
AleI CACnn'nnGTG
Cuts at: 1166 3621 3791 1166
Size: 2455 170 4192
Fragments arranged by size:
4192 2455 170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2463 1184
Size: 1279 5538
AlwNI CAG_nnn'CTG
Cuts at: 3268 3883 3916 4006 4560 6132 3268
Size: 615 33 90 554 1572 3953
Fragments arranged by size:
3953 1572 615 554 90 33
ApaI G_GGCC'C
Cuts at: 2138 2138
Size: 6817
ApaLI G'TGCA_C
Cuts at: 4874 6030 4874
Size: 1156 5661
ApoI r'AATT_y
Cuts at: 1493 3275 4126 4352 4504 6613 1493
Size: 1782 851 226 152 2109 1697
Fragments arranged by size:
2109 1782 1697 851 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5371 160
Size: 872 4339 1606
Fragments arranged by size:
4339 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
AvaI C'yCGr_G
Cuts at: 1870 1943 2674 2808 4142 1870
Size: 73 731 134 1334 4545
Fragments arranged by size:
4545 1334 731 134 73
AvrII C'CTAG_G
Cuts at: 4061 4061
Size: 6817
BamHI G'GATC_C
Cuts at: 4147 4514 4147
Size: 367 6450
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2065 2459 3482 4138 5593
Size: 325 217 627 278 394 1023 656 1455
Cuts at: 5593 618
Size: 1842
Fragments arranged by size:
1842 1455 1023 656 627 394 325 278
217
BanII G_rGCy'C
Cuts at: 1391 2138 3086 3606 3750 4136 1391
Size: 747 948 520 144 386 4072
Fragments arranged by size:
4072 948 747 520 386 144
BbeI G_GCGC'C
Cuts at: 2069 2069
Size: 6817
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5846
BbvCI CC'TCA_GC
Cuts at: 2577 3203 2577
Size: 626 6191
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2578 2612 5008 5042 1903
Size: 34 641 34 2396 34 3678
Fragments arranged by size:
3678 2396 641 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2470 4709 5919 2470
Size: 2239 1210 3368
Fragments arranged by size:
3368 2239 1210
BclI T'GATC_A
Cuts at: 1548 3270 1548
Size: 1722 5095
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3151 3436 5428
Size: 107 122 71 631 605 1479 285 1992
Cuts at: 5428 136
Size: 1525
Fragments arranged by size:
1992 1525 1479 631 605 285 122 107
71
BglII A'GATC_T
Cuts at: 6812 6812
Size: 6817
BlpI GC'TnA_GC
Cuts at: 3999 4234 3999
Size: 235 6582
Bme1580I G_kGCm'C
Cuts at: 1790 2138 3042 4878 6034 1790
Size: 348 904 1836 1156 2573
Fragments arranged by size:
2573 1836 1156 904 348
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6817
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2122 4548 5506
Size: 416 53 83 399 24 705 2426 958
Cuts at: 5506 442
Size: 1753
Fragments arranged by size:
2426 1753 958 705 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6817
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 3100 2371
Size: 729 6088
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2176 2733 3070 3863 3950 4127 5477 1239
Size: 937 557 337 793 87 177 1350 2579
Fragments arranged by size:
2579 1350 937 793 557 337 177 87
Bpu10I CC'TnA_GC
Cuts at: 2577 3203 2577
Size: 626 6191
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4942 5807 6105 4942
Size: 865 298 5654
Fragments arranged by size:
5654 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5480 882
Size: 617 3981 2219
Fragments arranged by size:
3981 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3037 4608 493
Size: 711 1833 1571 2702
Fragments arranged by size:
2702 1833 1571 711
BsaBI GATnn'nnATC
Cuts at: 6811 6811
Size: 6817
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2066 4624 5006 5641
Size: 53 83 186 936 533 2558 382 635
Cuts at: 5641 275
Size: 1451
Fragments arranged by size:
2558 1451 936 635 533 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5250 5648 5922 6069 6480 5250
Size: 398 274 147 411 5587
Fragments arranged by size:
5587 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2671 2701 3677 3707 1178
Size: 30 165 30 1268 30 976 30 4288
Fragments arranged by size:
4288 1268 976 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2954 3701 3758 3914 1186
Size: 1768 747 57 156 4089
Fragments arranged by size:
4089 1768 747 156 57
BseYI C'CCAG_C
Cuts at: 2149 3079 3709 3772 6020 6516 2149
Size: 930 630 63 2248 496 2450
Fragments arranged by size:
2450 2248 930 630 496 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2201 2267 2975 3599 1163
Size: 573 465 66 708 624 4381
Fragments arranged by size:
4381 708 624 573 465 66
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2082 4267 5028 6056 1540
Size: 275 186 81 2185 761 1028 2301
Fragments arranged by size:
2301 2185 1028 761 275 186 81
BsiHKAI G_wGCw'C
Cuts at: 3534 3606 3750 4878 4963 6034 3534
Size: 72 144 1128 85 1071 4317
Fragments arranged by size:
4317 1128 1071 144 85 72
BsmI GAATG_Cn'
Cuts at: 4328 4421 4328
Size: 93 6724
BsmBI CGTCTCn'nnnn_
Cuts at: 3650 3731 3650
Size: 81 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2138 3042 3086 3534 3606 3750 4136
Size: 399 348 904 44 448 72 144 386
Cuts at: 4136 4878 4963 6034 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 904 742 448 399 386 348
144 85 72 44
BspEI T'CCGG_A
Cuts at: 6480 6480
Size: 6817
BspHI T'CATG_A
Cuts at: 4706 6436 4706
Size: 1730 5087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4172 844
Size: 3328 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2664 3013 4704 1394
Size: 429 841 349 1691 3507
Fragments arranged by size:
3507 1691 841 429 349
BsrDI GCAATG_nn'
Cuts at: 65 1248 5312 5486 65
Size: 1183 4064 174 1396
Fragments arranged by size:
4064 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2068 3479 5461 5648 1700
Size: 110 258 1411 1982 187 2869
Fragments arranged by size:
2869 1982 1411 258 187 110
BsrGI T'GTAC_A
Cuts at: 96 3099 96
Size: 3003 3814
BssHII G'CGCG_C
Cuts at: 1925 2009 3904 3976 1925
Size: 84 1895 72 4766
Fragments arranged by size:
4766 1895 84 72
BssSI C'ACGA_G
Cuts at: 4877 5889 4877
Size: 1012 5805
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6817
BstBI TT'CG_AA
Cuts at: 5621 5621
Size: 6817
BstEII G'GTnAC_C
Cuts at: 1184 2687 3799 3832 1184
Size: 1503 1112 33 4169
Fragments arranged by size:
4169 1503 1112 33
BstXI CCAn_nnnn'nTGG
Cuts at: 2480 2480
Size: 6817
BstYI r'GATC_y
Cuts at: 1972 4147 4514 4899 4916 6368 6454 6466 6812
Size: 2175 367 385 17 1452 86 12 346
Cuts at: 6812 1972
Size: 1977
Fragments arranged by size:
2175 1977 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 3132 3828 4068 513
Size: 552 939 1128 696 240 3262
Fragments arranged by size:
3262 1128 939 696 552 240
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2382 487
Size: 683 56 1156 4922
Fragments arranged by size:
4922 1156 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 4333 5127 5147 2414
Size: 1919 794 20 4084
Fragments arranged by size:
4084 1919 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6782
DraI TTT'AAA
Cuts at: 4121 4121
Size: 6817
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5824 809
Size: 507 4508 1802
Fragments arranged by size:
4508 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2079 2407 2599
Size: 54 1425 50 275 18 249 328 192
Cuts at: 2599 3885 4264 5153 6782 8
Size: 1286 379 889 1629 43
Fragments arranged by size:
1629 1425 1286 889 379 328 275 249
192 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2079 4264 1537
Size: 275 267 2185 4090
Fragments arranged by size:
4090 2185 275 267
EarI CTCTTCn'nnn_
Cuts at: 1353 2496 3584 4747 1353
Size: 1143 1088 1163 3423
Fragments arranged by size:
3423 1163 1143 1088
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2890 5404 5778 5924 387
Size: 1064 138 1301 2514 374 146 1280
Fragments arranged by size:
2514 1301 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3604 3748 3604
Size: 144 6673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2176 2733 3070 3863 3950 4127 4880
Size: 393 544 557 337 793 87 177 753
Cuts at: 4880 5477 6264 6532 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 793 787 753 597 557 544 393
337 268 177 87
EcoO109I rG'GnC_Cy
Cuts at: 2135 3705 4102 2135
Size: 1570 397 4850
Fragments arranged by size:
4850 1570 397
EcoRI G'AATT_C
Cuts at: 4126 4504 4126
Size: 378 6439
FspI TGC'GCA
Cuts at: 3872 5323 3872
Size: 1451 5366
HaeII r_GCGC'y
Cuts at: 1678 2027 2069 2075 3428 5964 1678
Size: 349 42 6 1353 2536 2531
Fragments arranged by size:
2536 2531 1353 349 42 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2495 2527 2637 2669 3689
Size: 32 135 32 923 32 110 32 1020
Cuts at: 3689 3710 3721 3742 5458 5490 5532 5564 1373
Size: 21 11 21 1716 32 42 32 2626
Fragments arranged by size:
2626 1716 1020 923 135 110 42 32
32 32 32 32 32 21 21 11
HincII GTy'rAC
Cuts at: 669 1657 1938 4161 4407 669
Size: 988 281 2223 246 3079
Fragments arranged by size:
3079 2223 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2291 2733 748
Size: 1543 442 4832
Fragments arranged by size:
4832 1543 442
HpaI GTT'AAC
Cuts at: 4407 4407
Size: 6817
KasI G'GCGC_C
Cuts at: 2065 2065
Size: 6817
KpnI G_GTAC'C
Cuts at: 4142 4142
Size: 6817
MfeI C'AATT_G
Cuts at: 4416 4416
Size: 6817
MluI A'CGCG_T
Cuts at: 6693 6693
Size: 6817
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2538 2805 3832 5931 6115 1305
Size: 1233 267 1027 2099 184 2007
Fragments arranged by size:
2099 2007 1233 1027 267 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3887 10
Size: 54 1425 343 2055 2940
Fragments arranged by size:
2940 2055 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2900 3621 3668 3791 4775 5134 5293
Size: 648 1734 721 47 123 984 359 159
Cuts at: 5293 518
Size: 2042
Fragments arranged by size:
2042 1734 984 721 648 359 159 123
47
MspA1I CmG'CkG
Cuts at: 1606 3883 3973 4003 4233 4910 6058 6303 1606
Size: 2277 90 30 230 677 1148 245 2120
Fragments arranged by size:
2277 2120 1148 677 245 230 90 30
NaeI GCC'GGC
Cuts at: 2070 3481 2070
Size: 1411 5406
NarI GG'CG_CC
Cuts at: 2066 2066
Size: 6817
NcoI C'CATG_G
Cuts at: 513 1065 2004 3828 4068 513
Size: 552 939 1824 240 3262
Fragments arranged by size:
3262 1824 939 552 240
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6817
NgoMIV G'CCGG_C
Cuts at: 2068 3479 2068
Size: 1411 5406
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6817
NotI GC'GGCC_GC
Cuts at: 4264 4264
Size: 6817
NspI r_CATG'y
Cuts at: 1132 1229 3475 3877 4175 5720 1132
Size: 97 2246 402 298 1545 2229
Fragments arranged by size:
2246 2229 1545 402 298 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6817
PciI A'CATG_T
Cuts at: 5716 5716
Size: 6817
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6817
PfoI T'CCnGG_A
Cuts at: 2472 2553 3246 2472
Size: 81 693 6043
Fragments arranged by size:
6043 693 81
PmeI GTTT'AAAC
Cuts at: 4121 4121
Size: 6817
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4886 4918 6399 6431 4886
Size: 32 1481 32 5272
Fragments arranged by size:
5272 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3705 3705
Size: 6817
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6817
PsiI TTA'TAA
Cuts at: 4387 4387
Size: 6817
PspOMI G'GGCC_C
Cuts at: 2134 2134
Size: 6817
PstI C_TGCA'G
Cuts at: 830 2568 3222 3921 4169 830
Size: 1738 654 699 248 3478
Fragments arranged by size:
3478 1738 699 654 248
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6817
PvuII CAG'CTG
Cuts at: 3883 3973 4003 3883
Size: 90 30 6697
Fragments arranged by size:
6697 90 30
SacI G_AGCT'C
Cuts at: 3606 3750 3606
Size: 144 6673
SalI G'TCGA_C
Cuts at: 1936 4159 1936
Size: 2223 4594
SapI GCTCTTCn'nnn_
Cuts at: 2496 2496
Size: 6817
SbfI CC_TGCA'GG
Cuts at: 4169 4169
Size: 6817
ScaI AGT'ACT
Cuts at: 5065 6777 5065
Size: 1712 5105
SfcI C'TryA_G
Cuts at: 826 1045 2564 3218 3917 4165 5300 5981 6172
Size: 219 1519 654 699 248 1135 681 191
Cuts at: 6172 826
Size: 1471
Fragments arranged by size:
1519 1471 1135 699 681 654 248 219
191
SfoI GGC'GCC
Cuts at: 2067 2067
Size: 6817
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6817
SgrAI Cr'CCGG_yG
Cuts at: 1700 2068 1700
Size: 368 6449
SmaI CCC'GGG
Cuts at: 1872 2676 2810 4144 1872
Size: 804 134 1334 4545
Fragments arranged by size:
4545 1334 804 134
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4921 5822 6084 820
Size: 197 926 2978 901 262 1553
Fragments arranged by size:
2978 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4608 493
Size: 4115 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6817
SphI G_CATG'C
Cuts at: 1132 3475 3877 4175 1132
Size: 2343 402 298 3774
Fragments arranged by size:
3774 2343 402 298
SspI AAT'ATT
Cuts at: 5 52 4741 5
Size: 47 4689 2081
Fragments arranged by size:
4689 2081 47
StuI AGG'CCT
Cuts at: 1517 2549 1517
Size: 1032 5785
StyI C'CwwG_G
Cuts at: 513 1065 2004 2373 2913 3126 3828 4061 4068
Size: 552 939 369 540 213 702 233 7
Cuts at: 4068 4256 513
Size: 188 3074
Fragments arranged by size:
3074 939 702 552 540 369 233 213
188 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3833 4844 4861 5014 5199 5644 325
Size: 1255 2253 1011 17 153 185 445 1498
Fragments arranged by size:
2253 1498 1255 1011 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2319 2832 2859
Size: 274 80 33 51 1443 342 513 27
Cuts at: 2859 3099 5063 6775 96
Size: 240 1964 1712 138
Fragments arranged by size:
1964 1712 1443 513 342 274 240 138
80 51 33 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2762 2794 6777 6809 2762
Size: 32 3983 32 2770
Fragments arranged by size:
3983 2770 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3665 1316
Size: 2349 4468
XbaI T'CTAG_A
Cuts at: 4153 4153
Size: 6817
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2128 2371 2902 3520 2128
Size: 243 531 618 5425
Fragments arranged by size:
5425 618 531 243
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6817
XmaI C'CCGG_G
Cuts at: 1870 2674 2808 4142 1870
Size: 804 134 1334 4545
Fragments arranged by size:
4545 1334 804 134
XmnI GAAnn'nnTTC
Cuts at: 4946 4946
Size: 6817
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4625 276
Size: 53 83 186 936 3091 2468
Fragments arranged by size:
3091 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI PvuII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BfrBI BsiWI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NruI
NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI
SexAI SfiI SrfI SwaI