Restriction map for
pFN21AB9971
(Circular) (Six-base) MAPSORT of: pFN21AB9971.seq Check: 601 from: 1 to: 6367
pFN21AB9971 6367 bp
With 182 enzymes: SgfI *
April 15, 2010 09:33 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2358 2358
Size: 6367
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4177 278
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2313 3710 1937
Size: 376 1397 4594
Fragments arranged by size:
4594 1397 376
Acc65I G'GTAC_C
Cuts at: 3688 3688
Size: 6367
AclI AA'CG_TT
Cuts at: 1563 4494 4867 1563
Size: 2931 373 3063
Fragments arranged by size:
3063 2931 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2913 3442 4430 5814 6082 1632
Size: 1281 529 988 1384 268 1917
Fragments arranged by size:
1917 1384 1281 988 529 268
AflII C'TTAA_G
Cuts at: 820 1017 3635 820
Size: 197 2618 3552
Fragments arranged by size:
3552 2618 197
AflIII A'CryG_T
Cuts at: 1203 2601 5266 6243 1203
Size: 1398 2665 977 1327
Fragments arranged by size:
2665 1398 1327 977
AgeI A'CCGG_T
Cuts at: 5198 5198
Size: 6367
AhdI GACnn_n'nnGTC
Cuts at: 5096 5096
Size: 6367
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6367
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6367
AlwNI CAG_nnn'CTG
Cuts at: 2635 4110 5682 2635
Size: 1475 1572 3320
Fragments arranged by size:
3320 1572 1475
ApaI G_GGCC'C
Cuts at: 2241 2241
Size: 6367
ApaLI G'TGCA_C
Cuts at: 4424 5580 4424
Size: 1156 5211
ApoI r'AATT_y
Cuts at: 1493 2681 3232 3676 3902 4054 6163 1493
Size: 1188 551 444 226 152 2109 1697
Fragments arranged by size:
2109 1697 1188 551 444 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4921 160
Size: 872 3889 1606
Fragments arranged by size:
3889 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
AvaI C'yCGr_G
Cuts at: 1870 1943 2692 3166 3658 3692 1870
Size: 73 749 474 492 34 4545
Fragments arranged by size:
4545 749 492 474 73 34
BamHI G'GATC_C
Cuts at: 3697 4064 3697
Size: 367 6000
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3110 3662 3688 5143 618
Size: 325 217 627 1323 552 26 1455 1842
Fragments arranged by size:
1842 1455 1323 627 552 325 217 26
BanII G_rGCy'C
Cuts at: 1391 2201 2241 2699 2999 3096 3686 1391
Size: 810 40 458 300 97 590 4072
Fragments arranged by size:
4072 810 590 458 300 97 40
BbeI G_GCGC'C
Cuts at: 3114 3114
Size: 6367
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2116 3448 928
Size: 971 217 1332 3847
Fragments arranged by size:
3847 1332 971 217
BbvCI CC'TCA_GC
Cuts at: 2303 3464 2303
Size: 1161 5206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4558 4592 1903
Size: 34 50 34 2537 34 3678
Fragments arranged by size:
3678 2537 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4259 5469 4259
Size: 1210 5157
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6367
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2373 2785 4978
Size: 107 122 71 631 605 701 412 2193
Cuts at: 4978 136
Size: 1525
Fragments arranged by size:
2193 1525 701 631 605 412 122 107
71
BglII A'GATC_T
Cuts at: 6362 6362
Size: 6367
BlpI GC'TnA_GC
Cuts at: 2172 2177 2993 3366 3784 2172
Size: 5 816 373 418 4755
Fragments arranged by size:
4755 816 418 373 5
Bme1580I G_kGCm'C
Cuts at: 1790 2241 3665 4428 5584 1790
Size: 451 1424 763 1156 2573
Fragments arranged by size:
2573 1424 1156 763 451
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6367
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4098 5056 442
Size: 416 53 83 399 24 2681 958 1753
Fragments arranged by size:
2681 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2328 1056
Size: 1272 5095
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2048 2215 2683 5027 1239
Size: 809 167 468 2344 2579
Fragments arranged by size:
2579 2344 809 468 167
Bpu10I CC'TnA_GC
Cuts at: 2303 3464 2303
Size: 1161 5206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4492 5357 5655 4492
Size: 865 298 5204
Fragments arranged by size:
5204 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3542 3566 5030 882
Size: 617 2043 24 1464 2219
Fragments arranged by size:
2219 2043 1464 617 24
BsaAI yAC'GTr
Cuts at: 493 1204 4158 493
Size: 711 2954 2702
Fragments arranged by size:
2954 2702 711
BsaBI GATnn'nnATC
Cuts at: 3269 6361 3269
Size: 3092 3275
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3111 3585 4174 4556
Size: 53 83 186 936 1578 474 589 382
Cuts at: 4556 5191 275
Size: 635 1451
Fragments arranged by size:
1578 1451 936 635 589 474 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 4800 5198 5472 5619 6030 4800
Size: 398 274 147 411 5137
Fragments arranged by size:
5137 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3390 3420 1178
Size: 30 165 30 1987 30 4125
Fragments arranged by size:
4125 1987 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2222 1186
Size: 1036 5331
BseYI C'CCAG_C
Cuts at: 2356 2376 5570 6066 2356
Size: 20 3194 496 2657
Fragments arranged by size:
3194 2657 496 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2287 3086 3269 1163
Size: 573 551 799 183 4261
Fragments arranged by size:
4261 799 573 551 183
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3817 4578 5606 1540
Size: 275 186 1816 761 1028 2301
Fragments arranged by size:
2301 1816 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2580 3084 4428 4513 5584 2580
Size: 504 1344 85 1071 3363
Fragments arranged by size:
3363 1344 1071 504 85
BsmI GAATG_Cn'
Cuts at: 2476 3367 3878 3971 2476
Size: 891 511 93 4872
Fragments arranged by size:
4872 891 511 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2201 2241 2580 2699 2999 3084 3096
Size: 399 411 40 339 119 300 85 12
Cuts at: 3096 3665 3686 4428 4513 5584 1391
Size: 569 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 569 411 399 339 300
119 85 85 40 21 12
BspEI T'CCGG_A
Cuts at: 6030 6030
Size: 6367
BspHI T'CATG_A
Cuts at: 3261 4256 5986 3261
Size: 995 1730 3642
Fragments arranged by size:
3642 1730 995
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2023 2358 3291 3722 844
Size: 1179 335 933 431 3489
Fragments arranged by size:
3489 1179 933 431 335
BsrBI CCG'CTC
Cuts at: 1394 1823 3152 4254 1394
Size: 429 1329 1102 3507
Fragments arranged by size:
3507 1329 1102 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3333 4862 5036 65
Size: 1183 2085 1529 174 1396
Fragments arranged by size:
2085 1529 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5011 5198 1700
Size: 110 3201 187 2869
Fragments arranged by size:
3201 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6367
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6367
BssSI C'ACGA_G
Cuts at: 4427 5439 4427
Size: 1012 5355
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3484 794
Size: 2690 3677
BstBI TT'CG_AA
Cuts at: 5171 5171
Size: 6367
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6367
BstXI CCAn_nnnn'nTGG
Cuts at: 2113 2162 2189 2113
Size: 49 27 6291
Fragments arranged by size:
6291 49 27
BstYI r'GATC_y
Cuts at: 1972 3697 4064 4449 4466 5918 6004 6016 6362
Size: 1725 367 385 17 1452 86 12 346
Cuts at: 6362 1972
Size: 1977
Fragments arranged by size:
1977 1725 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3032 3032
Size: 6367
BtgI C'CryG_G
Cuts at: 513 1065 2013 2241 3219 3522 513
Size: 552 948 228 978 303 3358
Fragments arranged by size:
3358 978 948 552 303 228
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2902 487
Size: 683 56 1676 3952
Fragments arranged by size:
3952 1676 683 56
BtsI GCAGTG_nn'
Cuts at: 2149 3883 4677 4697 2149
Size: 1734 794 20 3819
Fragments arranged by size:
3819 1734 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6367
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6332
DraI TTT'AAA
Cuts at: 2955 3671 2955
Size: 716 5651
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5374 809
Size: 507 4058 1802
Fragments arranged by size:
4058 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2260 2331 2524
Size: 54 1425 50 275 18 430 71 193
Cuts at: 2524 3222 3316 3814 4703 6332 8
Size: 698 94 498 889 1629 43
Fragments arranged by size:
1629 1425 889 698 498 430 275 193
94 71 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3814 1537
Size: 275 2002 4090
Fragments arranged by size:
4090 2002 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2404 2621 2831 2967 4297 1353
Size: 1051 217 210 136 1330 3423
Fragments arranged by size:
3423 1330 1051 217 210 136
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2609 4954 5328 5474 387
Size: 1064 138 1020 2345 374 146 1280
Fragments arranged by size:
2345 1280 1064 1020 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2048 2215 2683 2913 3442 4430 5027
Size: 393 416 167 468 230 529 988 597
Cuts at: 5027 5814 6082 1239
Size: 787 268 1524
Fragments arranged by size:
1524 988 787 597 529 468 416 393
268 230 167
EcoNI CCTnn'n_nnAGG
Cuts at: 2627 2972 2627
Size: 345 6022
EcoO109I rG'GnC_Cy
Cuts at: 2237 2360 2632 3171 2237
Size: 123 272 539 5433
Fragments arranged by size:
5433 539 272 123
EcoRI G'AATT_C
Cuts at: 3676 4054 3676
Size: 378 5989
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2083 3061 2083
Size: 978 5389
FspI TGC'GCA
Cuts at: 4873 4873
Size: 6367
HaeII r_GCGC'y
Cuts at: 1678 2275 2301 2756 2791 3114 5514 1678
Size: 597 26 455 35 323 2400 2531
Fragments arranged by size:
2531 2400 597 455 323 35 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5008 5040 5082 5114 1373
Size: 32 135 32 3436 32 42 32 2626
Fragments arranged by size:
3436 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2560 3711 3957 669
Size: 988 281 622 1151 246 3079
Fragments arranged by size:
3079 1151 988 622 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6367
HpaI GTT'AAC
Cuts at: 3957 3957
Size: 6367
KasI G'GCGC_C
Cuts at: 3110 3110
Size: 6367
KpnI G_GTAC'C
Cuts at: 3692 3692
Size: 6367
MfeI C'AATT_G
Cuts at: 3966 3966
Size: 6367
MluI A'CGCG_T
Cuts at: 6243 6243
Size: 6367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5481 5665 1305
Size: 4176 184 2007
Fragments arranged by size:
4176 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2262 2333 3224 10
Size: 54 1425 343 430 71 891 3153
Fragments arranged by size:
3153 1425 891 430 343 71 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2111 2606 3053 3224 3305 4325 4684
Size: 648 945 495 447 171 81 1020 359
Cuts at: 4684 4843 518
Size: 159 2042
Fragments arranged by size:
2042 1020 945 648 495 447 359 171
159 81
MspA1I CmG'CkG
Cuts at: 1606 2243 2307 2356 2800 3120 3524 3783 4460
Size: 637 64 49 444 320 404 259 677
Cuts at: 4460 5608 5853 1606
Size: 1148 245 2120
Fragments arranged by size:
2120 1148 677 637 444 404 320 259
245 64 49
NarI GG'CG_CC
Cuts at: 3111 3111
Size: 6367
NcoI C'CATG_G
Cuts at: 513 1065 3219 513
Size: 552 2154 3661
Fragments arranged by size:
3661 2154 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6367
NheI G'CTAG_C
Cuts at: 1052 2324 1052
Size: 1272 5095
NotI GC'GGCC_GC
Cuts at: 3814 3814
Size: 6367
NspI r_CATG'y
Cuts at: 1132 1229 2605 3725 5270 1132
Size: 97 1376 1120 1545 2229
Fragments arranged by size:
2229 1545 1376 1120 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6367
PciI A'CATG_T
Cuts at: 2601 5266 2601
Size: 2665 3702
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6367
PfoI T'CCnGG_A
Cuts at: 2438 2438
Size: 6367
PmeI GTTT'AAAC
Cuts at: 3671 3671
Size: 6367
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4436 4468 5949 5981 4436
Size: 32 1481 32 4822
Fragments arranged by size:
4822 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2360 2360
Size: 6367
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6367
PsiI TTA'TAA
Cuts at: 3937 3937
Size: 6367
PspOMI G'GGCC_C
Cuts at: 2237 2237
Size: 6367
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3050 3082 3050
Size: 32 6335
PstI C_TGCA'G
Cuts at: 830 2631 2799 3719 830
Size: 1801 168 920 3478
Fragments arranged by size:
3478 1801 920 168
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6367
PvuII CAG'CTG
Cuts at: 2307 2800 2307
Size: 493 5874
SacII CC_GC'GG
Cuts at: 2244 3525 2244
Size: 1281 5086
SalI G'TCGA_C
Cuts at: 1936 3709 1936
Size: 1773 4594
SanDI GG'GwC_CC
Cuts at: 2360 2360
Size: 6367
SapI GCTCTTCn'nnn_
Cuts at: 2404 2621 2404
Size: 217 6150
SbfI CC_TGCA'GG
Cuts at: 3719 3719
Size: 6367
ScaI AGT'ACT
Cuts at: 4615 6327 4615
Size: 1712 4655
SexAI A'CCwGG_T
Cuts at: 2421 2421
Size: 6367
SfcI C'TryA_G
Cuts at: 826 1045 2627 2795 3715 4850 5531 5722 826
Size: 219 1582 168 920 1135 681 191 1471
Fragments arranged by size:
1582 1471 1135 920 681 219 191 168
SfoI GGC'GCC
Cuts at: 3112 3112
Size: 6367
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6367
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6367
SmaI CCC'GGG
Cuts at: 1872 3168 3694 1872
Size: 1296 526 4545
Fragments arranged by size:
4545 1296 526
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3635 4471 5372 5634 820
Size: 197 926 1692 836 901 262 1553
Fragments arranged by size:
1692 1553 926 901 836 262 197
SnaBI TAC'GTA
Cuts at: 493 4158 493
Size: 3665 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6367
SphI G_CATG'C
Cuts at: 1132 3725 1132
Size: 2593 3774
SspI AAT'ATT
Cuts at: 5 52 4291 5
Size: 47 4239 2081
Fragments arranged by size:
4239 2081 47
StuI AGG'CCT
Cuts at: 1517 2232 2252 1517
Size: 715 20 5632
Fragments arranged by size:
5632 715 20
StyI C'CwwG_G
Cuts at: 513 1065 2257 3219 3552 3595 3806 513
Size: 552 1192 962 333 43 211 3074
Fragments arranged by size:
3074 1192 962 552 333 211 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2368 4394 4411 4564 4749 5194 325
Size: 1255 788 2026 17 153 185 445 1498
Fragments arranged by size:
2026 1498 1255 788 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4613 6325 96
Size: 274 80 33 51 1443 2636 1712 138
Fragments arranged by size:
2636 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3390 3422 6327 6359 3390
Size: 32 2905 32 3398
Fragments arranged by size:
3398 2905 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6367
XbaI T'CTAG_A
Cuts at: 3703 3703
Size: 6367
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2011 2707 3199 2011
Size: 696 492 5179
Fragments arranged by size:
5179 696 492
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6367
XmaI C'CCGG_G
Cuts at: 1870 3166 3692 1870
Size: 1296 526 4545
Fragments arranged by size:
4545 1296 526
XmnI GAAnn'nnTTC
Cuts at: 4496 4496
Size: 6367
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4175 276
Size: 53 83 186 936 2641 2468
Fragments arranged by size:
2641 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI
PsrI PstI PvuI PvuII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI
BsmBI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI
SfiI SrfI SwaI