Restriction map for
pFN21AB9965
(Circular) (Six-base) MAPSORT of: pFN21AB9965.seq Check: 8770 from: 1 to: 6352
pFN21AB9965 6352 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4162 278
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2303 3695 1937
Size: 366 1392 4594
Fragments arranged by size:
4594 1392 366
Acc65I G'GTAC_C
Cuts at: 2204 3673 2204
Size: 1469 4883
AclI AA'CG_TT
Cuts at: 1563 4479 4852 1563
Size: 2916 373 3063
Fragments arranged by size:
3063 2916 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2400 2810 3441 4415 5799 6067 1632
Size: 768 410 631 974 1384 268 1917
Fragments arranged by size:
1917 1384 974 768 631 410 268
AfeI AGC'GCT
Cuts at: 3140 3140
Size: 6352
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6155
AflIII A'CryG_T
Cuts at: 1203 5251 6228 1203
Size: 4048 977 1327
Fragments arranged by size:
4048 1327 977
AgeI A'CCGG_T
Cuts at: 5183 5183
Size: 6352
AhdI GACnn_n'nnGTC
Cuts at: 5081 5081
Size: 6352
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6352
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3159 3309 1184
Size: 1975 150 4227
Fragments arranged by size:
4227 1975 150
AlwNI CAG_nnn'CTG
Cuts at: 4095 5667 4095
Size: 1572 4780
ApaLI G'TGCA_C
Cuts at: 3144 4409 5565 3144
Size: 1265 1156 3931
Fragments arranged by size:
3931 1265 1156
ApoI r'AATT_y
Cuts at: 1493 2399 2456 2793 3262 3410 3661 3887 4039
Size: 906 57 337 469 148 251 226 152
Cuts at: 4039 6148 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 906 469 337 251 226 152
148 57
AseI AT'TA_AT
Cuts at: 160 1032 4906 160
Size: 872 3874 1606
Fragments arranged by size:
3874 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
AvaI C'yCGr_G
Cuts at: 1870 1943 3677 1870
Size: 73 1734 4545
Fragments arranged by size:
4545 1734 73
BamHI G'GATC_C
Cuts at: 3682 4049 3682
Size: 367 5985
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2084 2204 3673 5128 618
Size: 325 217 627 297 120 1469 1455 1842
Fragments arranged by size:
1842 1469 1455 627 325 297 217 120
BanII G_rGCy'C
Cuts at: 1391 3671 1391
Size: 2280 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3368 3467 928
Size: 971 1469 99 3813
Fragments arranged by size:
3813 1469 971 99
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3209 3243 4543 4577 1903
Size: 34 1272 34 1300 34 3678
Fragments arranged by size:
3678 1300 1272 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4244 5454 4244
Size: 1210 5142
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6352
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2100 4963 136
Size: 107 122 71 631 605 428 2863 1525
Fragments arranged by size:
2863 1525 631 605 428 122 107 71
BglII A'GATC_T
Cuts at: 6347 6347
Size: 6352
BlpI GC'TnA_GC
Cuts at: 2438 2730 3769 2438
Size: 292 1039 5021
Fragments arranged by size:
5021 1039 292
Bme1580I G_kGCm'C
Cuts at: 1790 2087 2499 3148 4413 5569 1790
Size: 297 412 649 1265 1156 2573
Fragments arranged by size:
2573 1265 1156 649 412 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6352
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2210 2555 3390
Size: 416 53 83 399 24 793 345 835
Cuts at: 3390 4083 5041 442
Size: 693 958 1753
Fragments arranged by size:
1753 958 835 793 693 416 399 345
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3011 5012 1239
Size: 1772 2001 2579
Fragments arranged by size:
2579 2001 1772
Bpu10I CC'TnA_GC
Cuts at: 2391 2391
Size: 6352
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4477 5342 5640 4477
Size: 865 298 5189
Fragments arranged by size:
5189 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5015 882
Size: 617 3516 2219
Fragments arranged by size:
3516 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4143 493
Size: 711 2939 2702
Fragments arranged by size:
2939 2702 711
BsaBI GATnn'nnATC
Cuts at: 6346 6346
Size: 6352
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2172 4159 4541 5176
Size: 53 83 186 936 639 1987 382 635
Cuts at: 5176 275
Size: 1451
Fragments arranged by size:
1987 1451 936 639 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4785 5183 5457 5604 6015 4785
Size: 398 274 147 411 5122
Fragments arranged by size:
5122 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2132 2162 1178
Size: 30 165 30 729 30 5368
Fragments arranged by size:
5368 729 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2144 2156 2653 1186
Size: 958 12 497 4885
Fragments arranged by size:
4885 958 497 12
BseYI C'CCAG_C
Cuts at: 5555 6051 5555
Size: 496 5856
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2039 1163
Size: 573 303 5476
Fragments arranged by size:
5476 573 303
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3802 4563 5591 1540
Size: 275 186 1801 761 1028 2301
Fragments arranged by size:
2301 1801 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3148 4413 4498 5569 3148
Size: 1265 85 1071 3931
Fragments arranged by size:
3931 1265 1071 85
BsmI GAATG_Cn'
Cuts at: 2273 2377 3863 3956 2273
Size: 104 1486 93 4669
Fragments arranged by size:
4669 1486 104 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 2499 3148 3671 4413 4498 5569
Size: 399 297 412 649 523 742 85 1071
Cuts at: 5569 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 649 523 412 399 297
85
BspEI T'CCGG_A
Cuts at: 6015 6015
Size: 6352
BspHI T'CATG_A
Cuts at: 4241 5971 4241
Size: 1730 4622
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3707 844
Size: 2863 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4239 1394
Size: 429 2416 3507
Fragments arranged by size:
3507 2416 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4847 5021 65
Size: 1183 3599 174 1396
Fragments arranged by size:
3599 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2058 4996 5183 1700
Size: 110 248 2938 187 2869
Fragments arranged by size:
2938 2869 248 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6352
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6352
BssSI C'ACGA_G
Cuts at: 4412 5424 4412
Size: 1012 5340
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2091 794
Size: 1297 5055
BstBI TT'CG_AA
Cuts at: 2233 3601 5156 2233
Size: 1368 1555 3429
Fragments arranged by size:
3429 1555 1368
BstEII G'GTnAC_C
Cuts at: 1184 3031 3122 1184
Size: 1847 91 4414
Fragments arranged by size:
4414 1847 91
BstYI r'GATC_y
Cuts at: 1972 3222 3512 3682 4049 4434 4451 5903 5989
Size: 1250 290 170 367 385 17 1452 86
Cuts at: 5989 6001 6347 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1250 385 367 346 290 170
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2678 2678
Size: 6352
BtgI C'CryG_G
Cuts at: 513 1065 2004 2033 513
Size: 552 939 29 4832
Fragments arranged by size:
4832 939 552 29
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5613
Fragments arranged by size:
5613 683 56
BtsI GCAGTG_nn'
Cuts at: 3868 4662 4682 3868
Size: 794 20 5538
Fragments arranged by size:
5538 794 20
ClaI AT'CG_AT
Cuts at: 1554 2508 1554
Size: 954 5398
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2734 2769 529
Size: 35 2170 35 4112
Fragments arranged by size:
4112 2170 35 35
DraI TTT'AAA
Cuts at: 3656 3656
Size: 6352
DraIII CAC_nnn'GTG
Cuts at: 2091 2091
Size: 6352
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5359 809
Size: 507 4043 1802
Fragments arranged by size:
4043 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3799 4688 6317
Size: 54 1425 50 275 18 1969 889 1629
Cuts at: 6317 8
Size: 43
Fragments arranged by size:
1969 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3799 1537
Size: 275 1987 4090
Fragments arranged by size:
4090 1987 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2526 3206 4282 1353
Size: 1173 680 1076 3423
Fragments arranged by size:
3423 1173 1076 680
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4939 5313 5459 387
Size: 1064 138 3350 374 146 1280
Fragments arranged by size:
3350 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2400 2810 3011 3441 4415 5012 5799
Size: 393 768 410 201 430 974 597 787
Cuts at: 5799 6067 1239
Size: 268 1524
Fragments arranged by size:
1524 974 787 768 597 430 410 393
268 201
EcoO109I rG'GnC_Cy
Cuts at: 3091 3091
Size: 6352
EcoRI G'AATT_C
Cuts at: 2793 3661 4039 2793
Size: 868 378 5106
Fragments arranged by size:
5106 868 378
FspI TGC'GCA
Cuts at: 4858 4858
Size: 6352
HaeII r_GCGC'y
Cuts at: 1678 2557 3142 5499 1678
Size: 879 585 2357 2531
Fragments arranged by size:
2531 2357 879 585
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2285 2317 2699 2731 3214
Size: 32 135 32 713 32 382 32 483
Cuts at: 3214 3246 4993 5025 5067 5099 1373
Size: 32 1747 32 42 32 2626
Fragments arranged by size:
2626 1747 713 483 382 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3553 3696 3942 669
Size: 988 281 1615 143 246 3079
Fragments arranged by size:
3079 1615 988 281 246 143
HindIII A'AGCT_T
Cuts at: 748 2853 748
Size: 2105 4247
HpaI GTT'AAC
Cuts at: 3553 3942 3553
Size: 389 5963
KpnI G_GTAC'C
Cuts at: 2208 3677 2208
Size: 1469 4883
MfeI C'AATT_G
Cuts at: 2484 3951 2484
Size: 1467 4885
MluI A'CGCG_T
Cuts at: 6228 6228
Size: 6352
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2226 3485 5466 5650 1305
Size: 921 1259 1981 184 2007
Fragments arranged by size:
2007 1981 1259 921 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4530
Fragments arranged by size:
4530 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2324 3291 4310 4669 4828 518
Size: 648 1158 967 1019 359 159 2042
Fragments arranged by size:
2042 1158 1019 967 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2035 3768 4445 5593 5838 1606
Size: 429 1733 677 1148 245 2120
Fragments arranged by size:
2120 1733 1148 677 429 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4861
Fragments arranged by size:
4861 939 552
NdeI CA'TA_TG
Cuts at: 387 3538 387
Size: 3151 3201
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6352
NotI GC'GGCC_GC
Cuts at: 3799 3799
Size: 6352
NspI r_CATG'y
Cuts at: 1132 1229 3290 3710 5255 1132
Size: 97 2061 420 1545 2229
Fragments arranged by size:
2229 2061 1545 420 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6352
PciI A'CATG_T
Cuts at: 5251 5251
Size: 6352
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2430 2624 1108
Size: 1322 194 4836
Fragments arranged by size:
4836 1322 194
PfoI T'CCnGG_A
Cuts at: 2254 2254
Size: 6352
PmeI GTTT'AAAC
Cuts at: 3656 3656
Size: 6352
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4421 4453 5934 5966 4421
Size: 32 1481 32 4807
Fragments arranged by size:
4807 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6352
PsiI TTA'TAA
Cuts at: 3922 3922
Size: 6352
PstI C_TGCA'G
Cuts at: 830 3117 3704 830
Size: 2287 587 3478
Fragments arranged by size:
3478 2287 587
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6352
SacII CC_GC'GG
Cuts at: 2036 2036
Size: 6352
SalI G'TCGA_C
Cuts at: 1936 3694 1936
Size: 1758 4594
SapI GCTCTTCn'nnn_
Cuts at: 2526 2526
Size: 6352
SbfI CC_TGCA'GG
Cuts at: 3704 3704
Size: 6352
ScaI AGT'ACT
Cuts at: 2565 4600 6312 2565
Size: 2035 1712 2605
Fragments arranged by size:
2605 2035 1712
SfcI C'TryA_G
Cuts at: 826 1045 3113 3353 3700 4835 5516 5707 826
Size: 219 2068 240 347 1135 681 191 1471
Fragments arranged by size:
2068 1471 1135 681 347 240 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6352
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6352
SmaI CCC'GGG
Cuts at: 1872 3679 1872
Size: 1807 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4456 5357 5619 820
Size: 197 926 2513 901 262 1553
Fragments arranged by size:
2513 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4143 493
Size: 3650 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6352
SphI G_CATG'C
Cuts at: 1132 3290 3710 1132
Size: 2158 420 3774
Fragments arranged by size:
3774 2158 420
SspI AAT'ATT
Cuts at: 5 52 2991 4276 5
Size: 47 2939 1285 2081
Fragments arranged by size:
2939 2081 1285 47
StuI AGG'CCT
Cuts at: 1517 2906 1517
Size: 1389 4963
StyI C'CwwG_G
Cuts at: 513 1065 2004 3039 3791 513
Size: 552 939 1035 752 3074
Fragments arranged by size:
3074 1035 939 752 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2898 3141 4379 4396 4549 4734 5179
Size: 1255 1318 243 1238 17 153 185 445
Cuts at: 5179 325
Size: 1498
Fragments arranged by size:
1498 1318 1255 1238 445 243 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2563 4598 6310
Size: 274 80 33 51 1443 586 2035 1712
Cuts at: 6310 96
Size: 138
Fragments arranged by size:
2035 1712 1443 586 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2132 2164 3064 3096 3200 3232 6312 6344 2132
Size: 32 900 32 104 32 3080 32 2140
Fragments arranged by size:
3080 2140 900 104 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3367 1316
Size: 2051 4301
XbaI T'CTAG_A
Cuts at: 3688 3688
Size: 6352
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2585 2585
Size: 6352
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6352
XmaI C'CCGG_G
Cuts at: 1870 3677 1870
Size: 1807 4545
XmnI GAAnn'nnTTC
Cuts at: 2520 3016 4481 2520
Size: 496 1465 4391
Fragments arranged by size:
4391 1465 496
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4160 276
Size: 53 83 186 936 2626 2468
Fragments arranged by size:
2626 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI SacII SalI SapI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI
EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SanDI SexAI SfiI SfoI SrfI
SwaI