Restriction map for
pFN21AB9961
(Circular) (Six-base) MAPSORT of: pFN21AB9961.seq Check: 3707 from: 1 to: 6313
pFN21AB9961 6313 bp
With 182 enzymes: SgfI *
May 28, 2009 10:51 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 2876 3634 2876
Size: 758 5555
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2924 4376 5760 6028 1632
Size: 1292 1452 1384 268 1917
Fragments arranged by size:
1917 1452 1384 1292 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 3345 5212 6189 1203
Size: 2142 1867 977 1327
Fragments arranged by size:
2142 1867 1327 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 2290 3349 5042 2290
Size: 1059 1693 3561
Fragments arranged by size:
3561 1693 1059
AleI CACnn'nnGTG
Cuts at: 1166 2270 2741 2885 1166
Size: 1104 471 144 4594
Fragments arranged by size:
4594 1104 471 144
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3006 3101 1184
Size: 1822 95 4396
Fragments arranged by size:
4396 1822 95
AlwNI CAG_nnn'CTG
Cuts at: 2579 2689 2776 3311 4056 5628 2579
Size: 110 87 535 745 1572 3264
Fragments arranged by size:
3264 1572 745 535 110 87
ApaI G_GGCC'C
Cuts at: 2246 2246
Size: 6313
ApaLI G'TGCA_C
Cuts at: 2075 4370 5526 2075
Size: 2295 1156 2862
Fragments arranged by size:
2862 2295 1156
ApoI r'AATT_y
Cuts at: 1493 3281 3622 3848 4000 6109 1493
Size: 1788 341 226 152 2109 1697
Fragments arranged by size:
2109 1788 1697 341 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2515 2802 3476 3599 3638 1870
Size: 73 572 287 674 123 39 4545
Fragments arranged by size:
4545 674 572 287 123 73 39
BamHI G'GATC_C
Cuts at: 3360 3643 4010 3360
Size: 283 367 5663
Fragments arranged by size:
5663 367 283
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2046 2425 2558 2876 3275
Size: 325 217 627 259 379 133 318 399
Cuts at: 3275 3394 3634 5089 618
Size: 119 240 1455 1842
Fragments arranged by size:
1842 1455 627 399 379 325 318 259
240 217 133 119
BanII G_rGCy'C
Cuts at: 1391 2064 2187 2246 3012 3378 3588 3632 1391
Size: 673 123 59 766 366 210 44 4072
Fragments arranged by size:
4072 766 673 366 210 123 59 44
BbeI G_GCGC'C
Cuts at: 2050 2429 2050
Size: 379 5934
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3255 928
Size: 971 1356 3986
Fragments arranged by size:
3986 1356 971
BbvCI CC'TCA_GC
Cuts at: 2448 2963 3096 2448
Size: 515 133 5665
Fragments arranged by size:
5665 515 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6313
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2148 2174 4924
Size: 107 122 71 631 605 476 26 2750
Cuts at: 4924 136
Size: 1525
Fragments arranged by size:
2750 1525 631 605 476 122 107 71
26
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 2384 3730 2384
Size: 1346 4967
Bme1580I G_kGCm'C
Cuts at: 1790 2079 2246 2750 3399 4374 5530 1790
Size: 289 167 504 649 975 1156 2573
Fragments arranged by size:
2573 1156 975 649 504 289 167
BmgBI CAC'GTC
Cuts at: 1318 2754 3346 3576 1318
Size: 1436 592 230 4055
Fragments arranged by size:
4055 1436 592 230
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2662 3583 4044
Size: 416 53 83 399 24 1245 921 461
Cuts at: 4044 5002 442
Size: 958 1753
Fragments arranged by size:
1753 1245 958 921 461 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2078 2321 2840 2907 3288 3581 4973 1239
Size: 839 243 519 67 381 293 1392 2579
Fragments arranged by size:
2579 1392 839 519 381 293 243 67
Bpu10I CC'TnA_GC
Cuts at: 2448 2963 3096 2448
Size: 515 133 5665
Fragments arranged by size:
5665 515 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3047 4438 5303 5601 3047
Size: 1391 865 298 3759
Fragments arranged by size:
3759 1391 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2540 4976 882
Size: 617 1041 2436 2219
Fragments arranged by size:
2436 2219 1041 617
BsaAI yAC'GTr
Cuts at: 493 1204 2422 3142 4104 493
Size: 711 1218 720 962 2702
Fragments arranged by size:
2702 1218 962 720 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2047 2426 4120 4502
Size: 53 83 186 936 514 379 1694 382
Cuts at: 4502 5137 275
Size: 635 1451
Fragments arranged by size:
1694 1451 936 635 514 382 379 186
83 53
BsaWI w'CCGG_w
Cuts at: 3450 4746 5144 5418 5565 5976 3450
Size: 1296 398 274 147 411 3787
Fragments arranged by size:
3787 1296 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2642 2672 2905 2935 1178
Size: 30 165 30 1239 30 233 30 4556
Fragments arranged by size:
4556 1239 233 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2200 2465 3020 3539 1186
Size: 1014 265 555 519 3960
Fragments arranged by size:
3960 1014 555 519 265
BseYI C'CCAG_C
Cuts at: 2117 2153 2411 2830 5516 6012 2117
Size: 36 258 419 2686 496 2418
Fragments arranged by size:
2686 2418 496 419 258 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3002 3198 3470 1163
Size: 573 1266 196 272 4006
Fragments arranged by size:
4006 1266 573 272 196
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2136 2178 3763 4524 5552 1540
Size: 275 186 135 42 1585 761 1028 2301
Fragments arranged by size:
2301 1585 1028 761 275 186 135 42
BsiHKAI G_wGCw'C
Cuts at: 2079 3378 4374 4459 5530 2079
Size: 1299 996 85 1071 2862
Fragments arranged by size:
2862 1299 1071 996 85
BsiWI C'GTAC_G
Cuts at: 3138 3138
Size: 6313
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2064 2079 2187 2246 2750 3012 3378
Size: 399 274 15 108 59 504 262 366
Cuts at: 3378 3399 3588 3632 4374 4459 5530 1391
Size: 21 189 44 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 504 399 366 274 262
189 108 85 59 44 21 15
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 2952 4202 5932 2952
Size: 1250 1730 3333
Fragments arranged by size:
3333 1730 1250
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3093 3555 3668 844
Size: 2249 462 113 3489
Fragments arranged by size:
3489 2249 462 113
BsrBI CCG'CTC
Cuts at: 1394 1823 2332 2491 3148 3442 3529 4200 1394
Size: 429 509 159 657 294 87 671 3507
Fragments arranged by size:
3507 671 657 509 429 294 159 87
BsrDI GCAATG_nn'
Cuts at: 65 1248 4808 4982 65
Size: 1183 3560 174 1396
Fragments arranged by size:
3560 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2252 4957 5144 1700
Size: 110 442 2705 187 2869
Fragments arranged by size:
2869 2705 442 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6313
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6313
BssSI C'ACGA_G
Cuts at: 2531 4373 5385 2531
Size: 1842 1012 3459
Fragments arranged by size:
3459 1842 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6313
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 2272 2887 3190 2272
Size: 615 303 5395
Fragments arranged by size:
5395 615 303
BstYI r'GATC_y
Cuts at: 1972 2622 2796 3360 3643 4010 4395 4412 5864
Size: 650 174 564 283 367 385 17 1452
Cuts at: 5864 5950 5962 6308 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 650 564 385 367 346 283
174 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3127 3522 3127
Size: 395 5918
BtgI C'CryG_G
Cuts at: 513 1065 2004 2146 2172 513
Size: 552 939 142 26 4654
Fragments arranged by size:
4654 939 552 142 26
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 3829 4623 4643 3829
Size: 794 20 5499
Fragments arranged by size:
5499 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DraIII CAC_nnn'GTG
Cuts at: 2745 2745
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2311 5320 809
Size: 507 995 3009 1802
Fragments arranged by size:
3009 1802 995 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2133 2140 2175
Size: 54 1425 50 275 18 303 7 35
Cuts at: 2175 2250 2778 2889 3219 3322 3760 4649 6278
Size: 75 528 111 330 103 438 889 1629
Cuts at: 6278 8
Size: 43
Fragments arranged by size:
1629 1425 889 528 438 330 303 275
111 103 75 54 50 43 35 18
7
EagI C'GGCC_G
Cuts at: 1537 1812 2133 2175 3760 1537
Size: 275 321 42 1585 4090
Fragments arranged by size:
4090 1585 321 275 42
EarI CTCTTCn'nnn_
Cuts at: 1353 4243 1353
Size: 2890 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3526 4900 5274 5420 387
Size: 1064 138 1937 1374 374 146 1280
Fragments arranged by size:
1937 1374 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3376 3376
Size: 6313
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2078 2321 2840 2907 2924 3288 3581
Size: 393 446 243 519 67 17 364 293
Cuts at: 3581 4376 4973 5760 6028 1239
Size: 795 597 787 268 1524
Fragments arranged by size:
1524 795 787 597 519 446 393 364
293 268 243 67 17
EcoNI CCTnn'n_nnAGG
Cuts at: 2780 2939 2780
Size: 159 6154
EcoO109I rG'GnC_Cy
Cuts at: 2523 2599 2773 2865 3169 2523
Size: 76 174 92 304 5667
Fragments arranged by size:
5667 304 174 92 76
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FspI TGC'GCA
Cuts at: 4819 4819
Size: 6313
HaeII r_GCGC'y
Cuts at: 1678 2050 2085 2097 2429 5460 1678
Size: 372 35 12 332 3031 2531
Fragments arranged by size:
3031 2531 372 332 35 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2276 2308 3335 3367 4954
Size: 32 135 32 704 32 1027 32 1587
Cuts at: 4954 4986 5028 5060 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1587 1027 704 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2347 3657 3903 669
Size: 988 281 409 1310 246 3079
Fragments arranged by size:
3079 1310 988 409 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KasI G'GCGC_C
Cuts at: 2046 2425 2046
Size: 379 5934
KpnI G_GTAC'C
Cuts at: 2880 3638 2880
Size: 758 5555
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 6189 6189
Size: 6313
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2457 3321 5427 5611 1305
Size: 1152 864 2106 184 2007
Fragments arranged by size:
2106 2007 1152 864 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2780 2891 10
Size: 54 1425 343 948 111 3432
Fragments arranged by size:
3432 1425 948 343 111 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2270 2715 2741 2885 3191 4271 4630
Size: 648 1104 445 26 144 306 1080 359
Cuts at: 4630 4789 518
Size: 159 2042
Fragments arranged by size:
2042 1104 1080 648 445 359 306 159
144 26
MspA1I CmG'CkG
Cuts at: 1606 2044 2148 2174 2248 2640 2689 3100 3729
Size: 438 104 26 74 392 49 411 629
Cuts at: 3729 4406 5554 5799 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 629 438 411 392 245
104 74 49 26
NarI GG'CG_CC
Cuts at: 2047 2426 2047
Size: 379 5934
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4822
Fragments arranged by size:
4822 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 2175 3760 2175
Size: 1585 4728
NspI r_CATG'y
Cuts at: 1132 1229 3671 5216 1132
Size: 97 2442 1545 2229
Fragments arranged by size:
2442 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3404 1348
Size: 2056 4257
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3294 1108
Size: 2186 4127
PfoI T'CCnGG_A
Cuts at: 3558 3558
Size: 6313
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2523 2865 3169 2523
Size: 342 304 5667
Fragments arranged by size:
5667 342 304
PshAI GACnn'nnGTC
Cuts at: 1613 2482 1613
Size: 869 5444
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PspOMI G'GGCC_C
Cuts at: 2242 2242
Size: 6313
PstI C_TGCA'G
Cuts at: 830 2685 2940 3552 3665 830
Size: 1855 255 612 113 3478
Fragments arranged by size:
3478 1855 612 255 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2689 3100 2689
Size: 411 5902
SacI G_AGCT'C
Cuts at: 3378 3378
Size: 6313
SacII CC_GC'GG
Cuts at: 2149 2175 2149
Size: 26 6287
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 2940 3552 3665 2940
Size: 612 113 5588
Fragments arranged by size:
5588 612 113
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SexAI A'CCwGG_T
Cuts at: 3204 3432 3204
Size: 228 6085
SfcI C'TryA_G
Cuts at: 826 1045 2681 2936 3548 3661 4796 5477 5668
Size: 219 1636 255 612 113 1135 681 191
Cuts at: 5668 826
Size: 1471
Fragments arranged by size:
1636 1471 1135 681 612 255 219 191
113
SfiI GGCCn_nnn'nGGCC
Cuts at: 2148 2148
Size: 6313
SfoI GGC'GCC
Cuts at: 2048 2427 2048
Size: 379 5934
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 3601 3640 1872
Size: 1729 39 4545
Fragments arranged by size:
4545 1729 39
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3062 4417 5318 5580 820
Size: 197 926 1119 1355 901 262 1553
Fragments arranged by size:
1553 1355 1119 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4104 493
Size: 3611 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 2930 3554 1517
Size: 1413 624 4276
Fragments arranged by size:
4276 1413 624
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2595 2781 3752 513
Size: 552 939 24 567 186 971 3074
Fragments arranged by size:
3074 971 939 567 552 186 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2681 4340 4357 4510 4695 5140 325
Size: 1255 1101 1659 17 153 185 445 1498
Fragments arranged by size:
1659 1498 1255 1101 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3030 3258 4559
Size: 274 80 33 51 1443 1053 228 1301
Cuts at: 4559 6271 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1301 1053 274 228 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2905 2937 6273 6305 2905
Size: 32 3336 32 2913
Fragments arranged by size:
3336 2913 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2219 1316
Size: 903 5410
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2419 2843 3190 2419
Size: 424 347 5542
Fragments arranged by size:
5542 424 347
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6313
XmaI C'CCGG_G
Cuts at: 1870 3599 3638 1870
Size: 1729 39 4545
Fragments arranged by size:
4545 1729 39
XmnI GAAnn'nnTTC
Cuts at: 4442 4442
Size: 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SbfI
ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BplI
BsmBI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI RsrII SanDI
SapI SrfI SwaI