Restriction map for
pFN21AB9955
(Circular) (Six-base) MAPSORT of: pFN21AB9955.seq Check: 5970 from: 1 to: 6316
pFN21AB9955 6316 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3427 3427
Size: 6316
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4126 278
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3659 1937
Size: 1722 4594
Acc65I G'GTAC_C
Cuts at: 2085 2516 2867 3637 2085
Size: 431 351 770 4764
Fragments arranged by size:
4764 770 431 351
AclI AA'CG_TT
Cuts at: 1563 4443 4816 1563
Size: 2880 373 3063
Fragments arranged by size:
3063 2880 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2660 2987 3546 4379 5763 6031 1632
Size: 1028 327 559 833 1384 268 1917
Fragments arranged by size:
1917 1384 1028 833 559 327 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6119
AflIII A'CryG_T
Cuts at: 1203 3348 5215 6192 1203
Size: 2145 1867 977 1327
Fragments arranged by size:
2145 1867 1327 977
AgeI A'CCGG_T
Cuts at: 5147 5147
Size: 6316
AhdI GACnn_n'nnGTC
Cuts at: 2189 3352 5045 2189
Size: 1163 1693 3460
Fragments arranged by size:
3460 1693 1163
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6316
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2734 1184
Size: 1550 4766
AlwNI CAG_nnn'CTG
Cuts at: 4059 5631 4059
Size: 1572 4744
ApaI G_GGCC'C
Cuts at: 2662 2662
Size: 6316
ApaLI G'TGCA_C
Cuts at: 4373 5529 4373
Size: 1156 5160
ApoI r'AATT_y
Cuts at: 1493 2327 2616 3625 3851 4003 6112 1493
Size: 834 289 1009 226 152 2109 1697
Fragments arranged by size:
2109 1697 1009 834 289 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4870 160
Size: 872 3838 1606
Fragments arranged by size:
3838 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
AvaI C'yCGr_G
Cuts at: 1870 1943 2655 2805 3308 3641 1870
Size: 73 712 150 503 333 4545
Fragments arranged by size:
4545 712 503 333 150 73
BamHI G'GATC_C
Cuts at: 3646 4013 3646
Size: 367 5949
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2085 2516 2552 2867 3637
Size: 325 217 627 298 431 36 315 770
Cuts at: 3637 5092 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 770 627 431 325 315 298
217 36
BanII G_rGCy'C
Cuts at: 1391 2081 2662 3015 3088 3498 3591 3635 1391
Size: 690 581 353 73 410 93 44 4072
Fragments arranged by size:
4072 690 581 410 353 93 73 44
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3258 3573 928
Size: 971 1359 315 3671
Fragments arranged by size:
3671 1359 971 315
BbvCI CC'TCA_GC
Cuts at: 2112 2112
Size: 6316
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4507 4541 1903
Size: 34 2570 34 3678
Fragments arranged by size:
3678 2570 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2633 4208 5418 2633
Size: 1575 1210 3531
Fragments arranged by size:
3531 1575 1210
BclI T'GATC_A
Cuts at: 1548 2823 1548
Size: 1275 5041
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3311 4927 136
Size: 107 122 71 631 605 1639 1616 1525
Fragments arranged by size:
1639 1616 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3172 6311 3172
Size: 3139 3177
BlpI GC'TnA_GC
Cuts at: 2481 3733 2481
Size: 1252 5064
Bme1580I G_kGCm'C
Cuts at: 1790 2662 4377 5533 1790
Size: 872 1715 1156 2573
Fragments arranged by size:
2573 1715 1156 872
BmgBI CAC'GTC
Cuts at: 1318 3349 1318
Size: 2031 4285
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2656 2843 3586
Size: 416 53 83 399 24 1239 187 743
Cuts at: 3586 4047 5005 442
Size: 461 958 1753
Fragments arranged by size:
1753 1239 958 743 461 416 399 187
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6316
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2276 2276
Size: 6316
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2406 3452 4976 1239
Size: 1167 1046 1524 2579
Fragments arranged by size:
2579 1524 1167 1046
Bpu10I CC'TnA_GC
Cuts at: 2112 2112
Size: 6316
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2960 4441 5306 5604 2960
Size: 1481 865 298 3672
Fragments arranged by size:
3672 1481 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2589 2720 4979 882
Size: 617 1090 131 2259 2219
Fragments arranged by size:
2259 2219 1090 617 131
BsaAI yAC'GTr
Cuts at: 493 1204 4107 493
Size: 711 2903 2702
Fragments arranged by size:
2903 2702 711
BsaBI GATnn'nnATC
Cuts at: 6310 6310
Size: 6316
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4123 4505 5140 275
Size: 53 83 186 936 2590 382 635 1451
Fragments arranged by size:
2590 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4749 5147 5421 5568 5979 4749
Size: 398 274 147 411 5086
Fragments arranged by size:
5086 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2586 2616 1178
Size: 30 165 30 1183 30 4878
Fragments arranged by size:
4878 1183 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2066 2256 3023 1186
Size: 880 190 767 4479
Fragments arranged by size:
4479 880 767 190
BseYI C'CCAG_C
Cuts at: 2123 2405 5519 6015 2123
Size: 282 3114 496 2424
Fragments arranged by size:
3114 2424 496 282
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5743
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3766 4527 5555 1540
Size: 275 186 1765 761 1028 2301
Fragments arranged by size:
2301 1765 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2081 3088 3498 4377 4462 5533 2081
Size: 1007 410 879 85 1071 2864
Fragments arranged by size:
2864 1071 1007 879 410 85
BsiWI C'GTAC_G
Cuts at: 2234 2234
Size: 6316
BsmI GAATG_Cn'
Cuts at: 3827 3920 3827
Size: 93 6223
BsmBI CGTCTCn'nnnn_
Cuts at: 2220 2220
Size: 6316
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2081 2662 3015 3088 3498 3591 3635
Size: 399 291 581 353 73 410 93 44
Cuts at: 3635 4377 4462 5533 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 581 410 399 353 291
93 85 73 44
BspEI T'CCGG_A
Cuts at: 5979 5979
Size: 6316
BspHI T'CATG_A
Cuts at: 2955 3498 4205 5935 2955
Size: 543 707 1730 3336
Fragments arranged by size:
3336 1730 707 543
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2702 3427 3671 844
Size: 1858 725 244 3489
Fragments arranged by size:
3489 1858 725 244
BsrBI CCG'CTC
Cuts at: 1394 1823 2373 4203 1394
Size: 429 550 1830 3507
Fragments arranged by size:
3507 1830 550 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2024 4811 4985 65
Size: 1183 776 2787 174 1396
Fragments arranged by size:
2787 1396 1183 776 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4960 5147 1700
Size: 110 3150 187 2869
Fragments arranged by size:
3150 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2686 96
Size: 2590 3726
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6316
BssSI C'ACGA_G
Cuts at: 4376 5388 4376
Size: 1012 5304
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6316
BstBI TT'CG_AA
Cuts at: 5120 5120
Size: 6316
BstEII G'GTnAC_C
Cuts at: 1184 3382 1184
Size: 2198 4118
BstXI CCAn_nnnn'nTGG
Cuts at: 2081 2081
Size: 6316
BstYI r'GATC_y
Cuts at: 1972 3172 3646 4013 4398 4415 5867 5953 5965
Size: 1200 474 367 385 17 1452 86 12
Cuts at: 5965 6311 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1200 474 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4825
Fragments arranged by size:
4825 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5577
Fragments arranged by size:
5577 683 56
BtsI GCAGTG_nn'
Cuts at: 3003 3505 3832 4626 4646 3003
Size: 502 327 794 20 4673
Fragments arranged by size:
4673 794 502 327 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6316
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3087 3122 529
Size: 35 2523 35 3723
Fragments arranged by size:
3723 2523 35 35
DraI TTT'AAA
Cuts at: 3620 3620
Size: 6316
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2981 5323 809
Size: 507 1665 2342 1802
Fragments arranged by size:
2342 1802 1665 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2467 3763 4652
Size: 54 1425 50 275 18 637 1296 889
Cuts at: 4652 6281 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1296 889 637 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3763 1537
Size: 275 1951 4090
Fragments arranged by size:
4090 1951 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2215 2439 3076 3388 4246 1353
Size: 862 224 637 312 858 3423
Fragments arranged by size:
3423 862 858 637 312 224
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4903 5277 5423 387
Size: 1064 138 3314 374 146 1280
Fragments arranged by size:
3314 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2079 3086 3496 2079
Size: 1007 410 4899
Fragments arranged by size:
4899 1007 410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2406 2660 2987 3452 3546 4379 4976
Size: 393 774 254 327 465 94 833 597
Cuts at: 4976 5763 6031 1239
Size: 787 268 1524
Fragments arranged by size:
1524 833 787 774 597 465 393 327
268 254 94
EcoO109I rG'GnC_Cy
Cuts at: 2226 3520 2226
Size: 1294 5022
EcoRI G'AATT_C
Cuts at: 3625 4003 3625
Size: 378 5938
FspI TGC'GCA
Cuts at: 4822 4822
Size: 6316
HaeII r_GCGC'y
Cuts at: 1678 5463 1678
Size: 3785 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2175 2207 2586 2618 3164
Size: 32 135 32 603 32 379 32 546
Cuts at: 3164 3196 3338 3370 4957 4989 5031 5063 1373
Size: 32 142 32 1587 32 42 32 2626
Fragments arranged by size:
2626 1587 603 546 379 142 135 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2428 3660 3906 669
Size: 988 281 490 1232 246 3079
Fragments arranged by size:
3079 1232 988 490 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6316
HpaI GTT'AAC
Cuts at: 3906 3906
Size: 6316
KpnI G_GTAC'C
Cuts at: 2089 2520 2871 3641 2089
Size: 431 351 770 4764
Fragments arranged by size:
4764 770 431 351
MfeI C'AATT_G
Cuts at: 3915 3915
Size: 6316
MluI A'CGCG_T
Cuts at: 6192 6192
Size: 6316
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5430 5614 1305
Size: 4125 184 2007
Fragments arranged by size:
4125 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4494
Fragments arranged by size:
4494 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3194 4274 4633 4792 518
Size: 648 2028 1080 359 159 2042
Fragments arranged by size:
2042 2028 1080 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3732 4409 5557 5802 1606
Size: 2126 677 1148 245 2120
Fragments arranged by size:
2126 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4825
Fragments arranged by size:
4825 939 552
NdeI CA'TA_TG
Cuts at: 387 3212 387
Size: 2825 3491
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6316
NotI GC'GGCC_GC
Cuts at: 3763 3763
Size: 6316
NspI r_CATG'y
Cuts at: 1132 1229 3674 5219 1132
Size: 97 2445 1545 2229
Fragments arranged by size:
2445 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6316
PciI A'CATG_T
Cuts at: 5215 5215
Size: 6316
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3297 1108
Size: 2189 4127
PfoI T'CCnGG_A
Cuts at: 3561 3561
Size: 6316
PmeI GTTT'AAAC
Cuts at: 3620 3620
Size: 6316
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2063 2095 4385 4417 5898 5930 2063
Size: 32 2290 32 1481 32 2449
Fragments arranged by size:
2449 2290 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2226 3520 2226
Size: 1294 5022
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6316
PsiI TTA'TAA
Cuts at: 2613 3886 2613
Size: 1273 5043
PspOMI G'GGCC_C
Cuts at: 2658 2658
Size: 6316
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3314 3346 3314
Size: 32 6284
PstI C_TGCA'G
Cuts at: 830 2713 2820 3133 3668 830
Size: 1883 107 313 535 3478
Fragments arranged by size:
3478 1883 535 313 107
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6316
SacI G_AGCT'C
Cuts at: 2081 3088 3498 2081
Size: 1007 410 4899
Fragments arranged by size:
4899 1007 410
SalI G'TCGA_C
Cuts at: 1936 3658 1936
Size: 1722 4594
SapI GCTCTTCn'nnn_
Cuts at: 3076 3076
Size: 6316
SbfI CC_TGCA'GG
Cuts at: 3668 3668
Size: 6316
ScaI AGT'ACT
Cuts at: 3548 4564 6276 3548
Size: 1016 1712 3588
Fragments arranged by size:
3588 1712 1016
SexAI A'CCwGG_T
Cuts at: 3601 3612 3601
Size: 11 6305
SfcI C'TryA_G
Cuts at: 826 1045 2709 2816 3129 3451 3664 4799 5480
Size: 219 1664 107 313 322 213 1135 681
Cuts at: 5480 5671 826
Size: 191 1471
Fragments arranged by size:
1664 1471 1135 681 322 313 219 213
191 107
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6316
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6316
SmaI CCC'GGG
Cuts at: 1872 2657 3643 1872
Size: 785 986 4545
Fragments arranged by size:
4545 986 785
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2805 2939 4420 5321 5583 820
Size: 197 926 862 134 1481 901 262 1553
Fragments arranged by size:
1553 1481 926 901 862 262 197 134
SnaBI TAC'GTA
Cuts at: 493 4107 493
Size: 3614 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6316
SphI G_CATG'C
Cuts at: 1132 3674 1132
Size: 2542 3774
SspI AAT'ATT
Cuts at: 5 52 2337 4240 5
Size: 47 2285 1903 2081
Fragments arranged by size:
2285 2081 1903 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6316
StyI C'CwwG_G
Cuts at: 513 1065 2004 2179 2756 3755 513
Size: 552 939 175 577 999 3074
Fragments arranged by size:
3074 999 939 577 552 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4343 4360 4513 4698 5143 325
Size: 1255 2763 17 153 185 445 1498
Fragments arranged by size:
2763 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2686 3261 3546
Size: 274 80 33 51 1443 709 575 285
Cuts at: 3546 4562 6274 96
Size: 1016 1712 138
Fragments arranged by size:
1712 1443 1016 709 575 285 274 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2238 2270 2553 2585 6276 6308 2238
Size: 32 283 32 3691 32 2246
Fragments arranged by size:
3691 2246 283 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2600 1316
Size: 1284 5032
XbaI T'CTAG_A
Cuts at: 3652 3652
Size: 6316
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2413 3599 2413
Size: 1186 5130
XhoI C'TCGA_G
Cuts at: 1943 2805 1943
Size: 862 5454
XmaI C'CCGG_G
Cuts at: 1870 2655 3641 1870
Size: 785 986 4545
Fragments arranged by size:
4545 986 785
XmnI GAAnn'nnTTC
Cuts at: 2328 2673 4445 2328
Size: 345 1772 4199
Fragments arranged by size:
4199 1772 345
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4124 276
Size: 53 83 186 936 2590 2468
Fragments arranged by size:
2590 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI
PvuI SacI SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BfrBI BstZ17I
Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI
PvuII RsrII SacII SanDI SfiI SfoI SrfI SwaI