Restriction map for
pFN21AB9954
(Circular) (Six-base) MAPSORT of: pFN21AB9954.seq Check: 2901 from: 1 to: 6736
pFN21AB9954 6736 bp
With 182 enzymes: SgfI *
April 15, 2010 09:23 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3844 3844
Size: 6736
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4546 278
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4079 1937
Size: 2142 4594
Acc65I G'GTAC_C
Cuts at: 4057 4057
Size: 6736
AclI AA'CG_TT
Cuts at: 1563 4863 5236 1563
Size: 3300 373 3063
Fragments arranged by size:
3300 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2169 2620 2921 3353 4799 6183 6451 1632
Size: 537 451 301 432 1446 1384 268 1917
Fragments arranged by size:
1917 1446 1384 537 451 432 301 268
AfeI AGC'GCT
Cuts at: 2170 2170
Size: 6736
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6539
AflIII A'CryG_T
Cuts at: 1203 5635 6612 1203
Size: 4432 977 1327
Fragments arranged by size:
4432 1327 977
AgeI A'CCGG_T
Cuts at: 5567 5567
Size: 6736
AhdI GACnn_n'nnGTC
Cuts at: 5465 5465
Size: 6736
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6736
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2232 4005 1184
Size: 1048 1773 3915
Fragments arranged by size:
3915 1773 1048
AlwNI CAG_nnn'CTG
Cuts at: 4479 6051 4479
Size: 1572 5164
ApaLI G'TGCA_C
Cuts at: 4793 5949 4793
Size: 1156 5580
ApoI r'AATT_y
Cuts at: 1493 2332 2489 2684 2753 3033 3267 3355 3405
Size: 839 157 195 69 280 234 88 50
Cuts at: 3405 3412 4009 4045 4271 4423 6532 1493
Size: 7 597 36 226 152 2109 1697
Fragments arranged by size:
2109 1697 839 597 280 234 226 195
157 152 88 69 50 36 7
AseI AT'TA_AT
Cuts at: 160 1032 5290 160
Size: 872 4258 1606
Fragments arranged by size:
4258 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
AvaI C'yCGr_G
Cuts at: 1870 1943 4061 1870
Size: 73 2118 4545
Fragments arranged by size:
4545 2118 73
BamHI G'GATC_C
Cuts at: 3800 4066 4433 3800
Size: 266 367 6103
Fragments arranged by size:
6103 367 266
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4057 5512 618
Size: 325 217 627 2270 1455 1842
Fragments arranged by size:
2270 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4055 1391
Size: 2664 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2019 2157 2789 3876 928
Size: 971 120 138 632 1087 3788
Fragments arranged by size:
3788 1087 971 632 138 120
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4927 4961 1903
Size: 34 2990 34 3678
Fragments arranged by size:
3678 2990 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3613 4628 5838 3613
Size: 1015 1210 4511
Fragments arranged by size:
4511 1210 1015
BclI T'GATC_A
Cuts at: 1548 2998 3499 3814 1548
Size: 1450 501 315 4470
Fragments arranged by size:
4470 1450 501 315
BfrBI ATG'CAT
Cuts at: 2735 2975 3010 2735
Size: 240 35 6461
Fragments arranged by size:
6461 240 35
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2109 2187 5347
Size: 107 122 71 631 605 437 78 3160
Cuts at: 5347 136
Size: 1525
Fragments arranged by size:
3160 1525 631 605 437 122 107 78
71
BglII A'GATC_T
Cuts at: 3628 6731 3628
Size: 3103 3633
BlpI GC'TnA_GC
Cuts at: 3276 4153 3276
Size: 877 5859
Bme1580I G_kGCm'C
Cuts at: 1790 4797 5953 1790
Size: 3007 1156 2573
Fragments arranged by size:
3007 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 2591 1318
Size: 1273 5463
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4467 5425 442
Size: 416 53 83 399 24 3050 958 1753
Fragments arranged by size:
3050 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6736
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2529 2529
Size: 6736
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5396 1239
Size: 4157 2579
Bpu10I CC'TnA_GC
Cuts at: 2965 2965
Size: 6736
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2904 4861 5726 6024 2904
Size: 1957 865 298 3616
Fragments arranged by size:
3616 1957 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2265 5399 882
Size: 617 766 3134 2219
Fragments arranged by size:
3134 2219 766 617
BsaAI yAC'GTr
Cuts at: 493 1204 4527 493
Size: 711 3323 2702
Fragments arranged by size:
3323 2702 711
BsaBI GATnn'nnATC
Cuts at: 2895 6730 2895
Size: 3835 2901
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3196 4543 4925 5560
Size: 53 83 186 936 1663 1347 382 635
Cuts at: 5560 275
Size: 1451
Fragments arranged by size:
1663 1451 1347 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 5169 5567 5841 5988 6399 5169
Size: 398 274 147 411 5506
Fragments arranged by size:
5506 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2607 2637 1178
Size: 30 165 30 1204 30 5277
Fragments arranged by size:
5277 1204 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6736
BseYI C'CCAG_C
Cuts at: 2278 5939 6435 2278
Size: 3661 496 2579
Fragments arranged by size:
3661 2579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3453 1163
Size: 573 1717 4446
Fragments arranged by size:
4446 1717 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4186 4947 5975 1540
Size: 275 186 2185 761 1028 2301
Fragments arranged by size:
2301 2185 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2416 3829 4797 4882 5953 2416
Size: 1413 968 85 1071 3199
Fragments arranged by size:
3199 1413 1071 968 85
BsmI GAATG_Cn'
Cuts at: 4247 4340 4247
Size: 93 6643
BsmBI CGTCTCn'nnnn_
Cuts at: 2672 2672
Size: 6736
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2416 3829 4055 4797 4882 5953 1391
Size: 399 626 1413 226 742 85 1071 2174
Fragments arranged by size:
2174 1413 1071 742 626 399 226 85
BspEI T'CCGG_A
Cuts at: 6399 6399
Size: 6736
BspHI T'CATG_A
Cuts at: 2242 2656 2995 4625 6355 2242
Size: 414 339 1630 1730 2623
Fragments arranged by size:
2623 1730 1630 414 339
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3844 4091 844
Size: 3000 247 3489
Fragments arranged by size:
3489 3000 247
BsrBI CCG'CTC
Cuts at: 1394 1823 2579 4623 1394
Size: 429 756 2044 3507
Fragments arranged by size:
3507 2044 756 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2197 3012 5231 5405 65
Size: 1183 949 815 2219 174 1396
Fragments arranged by size:
2219 1396 1183 949 815 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3198 5380 5567 1700
Size: 110 1388 2182 187 2869
Fragments arranged by size:
2869 2182 1388 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6736
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6736
BssSI C'ACGA_G
Cuts at: 4796 5808 4796
Size: 1012 5724
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6736
BstBI TT'CG_AA
Cuts at: 5540 5540
Size: 6736
BstEII G'GTnAC_C
Cuts at: 1184 2570 1184
Size: 1386 5350
BstYI r'GATC_y
Cuts at: 1972 3628 3800 4066 4433 4818 4835 6287 6373
Size: 1656 172 266 367 385 17 1452 86
Cuts at: 6373 6385 6731 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1656 1452 385 367 346 266 172
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2526 513
Size: 552 1461 4723
Fragments arranged by size:
4723 1461 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5997
Fragments arranged by size:
5997 683 56
BtsI GCAGTG_nn'
Cuts at: 4252 5046 5066 4252
Size: 794 20 5922
Fragments arranged by size:
5922 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6736
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6701
DraI TTT'AAA
Cuts at: 4006 4040 4006
Size: 34 6702
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5743 809
Size: 507 4427 1802
Fragments arranged by size:
4427 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4183 5072 6701
Size: 54 1425 50 275 18 2353 889 1629
Cuts at: 6701 8
Size: 43
Fragments arranged by size:
2353 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4183 1537
Size: 275 2371 4090
Fragments arranged by size:
4090 2371 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2704 2944 4666 1353
Size: 1351 240 1722 3423
Fragments arranged by size:
3423 1722 1351 240
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5323 5697 5843 387
Size: 1064 138 3734 374 146 1280
Fragments arranged by size:
3734 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2169 2620 2921 3353 4799 5396 6183
Size: 393 537 451 301 432 1446 597 787
Cuts at: 6183 6451 1239
Size: 268 1524
Fragments arranged by size:
1524 1446 787 597 537 451 432 393
301 268
EcoRI G'AATT_C
Cuts at: 3355 3405 4045 4423 3355
Size: 50 640 378 5668
Fragments arranged by size:
5668 640 378 50
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3334 3334
Size: 6736
FspI TGC'GCA
Cuts at: 5242 5242
Size: 6736
HaeII r_GCGC'y
Cuts at: 1678 2172 5883 1678
Size: 494 3711 2531
Fragments arranged by size:
3711 2531 494
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3069 3101 3484 3516 3640
Size: 32 135 32 1497 32 383 32 124
Cuts at: 3640 3672 5377 5409 5451 5483 1373
Size: 32 1705 32 42 32 2626
Fragments arranged by size:
2626 1705 1497 383 135 124 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3234 3325 4080 4326 669
Size: 988 281 1296 91 755 246 3079
Fragments arranged by size:
3079 1296 988 755 281 246 91
HindIII A'AGCT_T
Cuts at: 748 2046 748
Size: 1298 5438
HpaI GTT'AAC
Cuts at: 3234 4326 3234
Size: 1092 5644
KpnI G_GTAC'C
Cuts at: 4061 4061
Size: 6736
MfeI C'AATT_G
Cuts at: 2913 4335 2913
Size: 1422 5314
MluI A'CGCG_T
Cuts at: 6612 6612
Size: 6736
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2122 2939 3878 3978 5850 6034 1305
Size: 817 817 939 100 1872 184 2007
Fragments arranged by size:
2007 1872 939 817 817 184 100
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4914
Fragments arranged by size:
4914 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2214 2241 4694 5053 5212 518
Size: 648 1048 27 2453 359 159 2042
Fragments arranged by size:
2453 2042 1048 648 359 159 27
MspA1I CmG'CkG
Cuts at: 1606 2278 2702 4152 4829 5977 6222 1606
Size: 672 424 1450 677 1148 245 2120
Fragments arranged by size:
2120 1450 1148 677 672 424 245
NaeI GCC'GGC
Cuts at: 3200 3200
Size: 6736
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 6184
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6736
NgoMIV G'CCGG_C
Cuts at: 3198 3198
Size: 6736
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6736
NotI GC'GGCC_GC
Cuts at: 4183 4183
Size: 6736
NsiI A_TGCA'T
Cuts at: 2737 2977 3012 2737
Size: 240 35 6461
Fragments arranged by size:
6461 240 35
NspI r_CATG'y
Cuts at: 1132 1229 2958 4094 5639 1132
Size: 97 1729 1136 1545 2229
Fragments arranged by size:
2229 1729 1545 1136 97
PasI CC'CwG_GG
Cuts at: 1348 3898 1348
Size: 2550 4186
PciI A'CATG_T
Cuts at: 5635 5635
Size: 6736
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6736
PmeI GTTT'AAAC
Cuts at: 4040 4040
Size: 6736
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4805 4837 6318 6350 4805
Size: 32 1481 32 5191
Fragments arranged by size:
5191 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6736
PsiI TTA'TAA
Cuts at: 4306 4306
Size: 6736
PstI C_TGCA'G
Cuts at: 830 2134 2520 4088 830
Size: 1304 386 1568 3478
Fragments arranged by size:
3478 1568 1304 386
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6736
PvuII CAG'CTG
Cuts at: 2278 2702 2278
Size: 424 6312
SalI G'TCGA_C
Cuts at: 1936 4078 1936
Size: 2142 4594
SapI GCTCTTCn'nnn_
Cuts at: 2704 2944 2704
Size: 240 6496
SbfI CC_TGCA'GG
Cuts at: 4088 4088
Size: 6736
ScaI AGT'ACT
Cuts at: 4984 6696 4984
Size: 1712 5024
SfcI C'TryA_G
Cuts at: 826 1045 2130 2516 2820 3118 4084 5219 5900
Size: 219 1085 386 304 298 966 1135 681
Cuts at: 5900 6091 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1085 966 681 386 304 298
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6736
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6736
SmaI CCC'GGG
Cuts at: 1872 4063 1872
Size: 2191 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2919 4840 5741 6003 820
Size: 197 926 976 1921 901 262 1553
Fragments arranged by size:
1921 1553 976 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4527 493
Size: 4034 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6736
SphI G_CATG'C
Cuts at: 1132 4094 1132
Size: 2962 3774
SspI AAT'ATT
Cuts at: 5 52 4660 5
Size: 47 4608 2081
Fragments arranged by size:
4608 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6736
StyI C'CwwG_G
Cuts at: 513 1065 2067 2626 2725 4175 513
Size: 552 1002 559 99 1450 3074
Fragments arranged by size:
3074 1450 1002 559 552 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2558 2589 2829 4763 4780 4933 5118
Size: 1255 978 31 240 1934 17 153 185
Cuts at: 5118 5563 325
Size: 445 1498
Fragments arranged by size:
1934 1498 1255 978 445 240 185 153
31 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4982 6694 96
Size: 274 80 33 51 1443 3005 1712 138
Fragments arranged by size:
3005 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2607 2639 3415 3447 3835 3867 6696 6728 2607
Size: 32 776 32 388 32 2829 32 2615
Fragments arranged by size:
2829 2615 776 388 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6736
XbaI T'CTAG_A
Cuts at: 4072 4072
Size: 6736
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6736
XmaI C'CCGG_G
Cuts at: 1870 4061 1870
Size: 2191 4545
XmnI GAAnn'nnTTC
Cuts at: 4865 4865
Size: 6736
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4544 276
Size: 53 83 186 936 3010 2468
Fragments arranged by size:
3010 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NsiI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI PvuII SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FseI FspAI KasI NarI NruI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI