Restriction map for
pFN21AB9953
(Circular) (Six-base) MAPSORT of: pFN21AB9953.seq Check: 1859 from: 1 to: 6313
pFN21AB9953 6313 bp
With 182 enzymes: SgfI *
April 15, 2010 09:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2134 3145 3187 2134
Size: 1011 42 5260
Fragments arranged by size:
5260 1011 42
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4123 278
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3656 1937
Size: 1719 4594
Acc65I G'GTAC_C
Cuts at: 3634 3634
Size: 6313
AclI AA'CG_TT
Cuts at: 1563 4440 4813 1563
Size: 2877 373 3063
Fragments arranged by size:
3063 2877 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2720 4376 5760 6028 1632
Size: 1088 1656 1384 268 1917
Fragments arranged by size:
1917 1656 1384 1088 268
AfeI AGC'GCT
Cuts at: 2534 2534
Size: 6313
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6116
AflIII A'CryG_T
Cuts at: 1203 2328 5212 6189 1203
Size: 1125 2884 977 1327
Fragments arranged by size:
2884 1327 1125 977
AgeI A'CCGG_T
Cuts at: 5144 5144
Size: 6313
AhdI GACnn_n'nnGTC
Cuts at: 5042 5042
Size: 6313
AleI CACnn'nnGTG
Cuts at: 1166 2580 1166
Size: 1414 4899
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3173 1184
Size: 1989 4324
AlwNI CAG_nnn'CTG
Cuts at: 2359 2401 2740 2794 2851 2875 2992 3223 3256
Size: 42 339 54 57 24 117 231 33
Cuts at: 3256 3334 4056 5628 2359
Size: 78 722 1572 3044
Fragments arranged by size:
3044 1572 722 339 231 117 78 57
54 42 33 24
ApaLI G'TGCA_C
Cuts at: 4370 5526 4370
Size: 1156 5157
ApoI r'AATT_y
Cuts at: 1493 3622 3848 4000 6109 1493
Size: 2129 226 152 2109 1697
Fragments arranged by size:
2129 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4867 160
Size: 872 3835 1606
Fragments arranged by size:
3835 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
AvaI C'yCGr_G
Cuts at: 1870 1943 2243 2781 3120 3467 3638 1870
Size: 73 300 538 339 347 171 4545
Fragments arranged by size:
4545 538 347 339 300 171 73
BamHI G'GATC_C
Cuts at: 3643 4010 3643
Size: 367 5946
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2594 3483 3634 5089 618
Size: 325 217 627 807 889 151 1455 1842
Fragments arranged by size:
1842 1455 889 807 627 325 217 151
BanII G_rGCy'C
Cuts at: 1391 2259 2420 2529 3278 3318 3386 3611 3632
Size: 868 161 109 749 40 68 225 21
Cuts at: 3632 1391
Size: 4072
Fragments arranged by size:
4072 868 749 225 161 109 68 40
21
BbeI G_GCGC'C
Cuts at: 2598 3487 2598
Size: 889 5424
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2302 928
Size: 971 403 4939
Fragments arranged by size:
4939 971 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4504 4538 1903
Size: 34 2567 34 3678
Fragments arranged by size:
3678 2567 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4205 5415 4205
Size: 1210 5103
BclI T'GATC_A
Cuts at: 1548 2319 1548
Size: 771 5542
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2226 3372 4924
Size: 107 122 71 631 605 554 1146 1552
Cuts at: 4924 136
Size: 1525
Fragments arranged by size:
1552 1525 1146 631 605 554 122 107
71
BglII A'GATC_T
Cuts at: 6308 6308
Size: 6313
BlpI GC'TnA_GC
Cuts at: 2150 2414 3730 2150
Size: 264 1316 4733
Fragments arranged by size:
4733 1316 264
Bme1580I G_kGCm'C
Cuts at: 1790 4374 5530 1790
Size: 2584 1156 2573
Fragments arranged by size:
2584 2573 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6313
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4044 5002 442
Size: 416 53 83 399 24 2627 958 1753
Fragments arranged by size:
2627 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6313
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2066 2273 2648 2759 2816 2873 3278 3356
Size: 827 207 375 111 57 57 405 78
Cuts at: 3356 4973 1239
Size: 1617 2579
Fragments arranged by size:
2579 1617 827 405 375 207 111 78
57 57
Bpu10I CC'TnA_GC
Cuts at: 2705 3242 3272 3449 2705
Size: 537 30 177 5569
Fragments arranged by size:
5569 537 177 30
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2480 2513 2627 4438 5303 5601 2480
Size: 33 114 1811 865 298 3192
Fragments arranged by size:
3192 1811 865 298 114 33
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4976 882
Size: 617 3477 2219
Fragments arranged by size:
3477 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2233 3002 4104 493
Size: 711 1029 769 1102 2702
Fragments arranged by size:
2702 1102 1029 769 711
BsaBI GATnn'nnATC
Cuts at: 6307 6307
Size: 6313
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2595 2947 3484 4120
Size: 53 83 186 936 1062 352 537 636
Cuts at: 4120 4502 5137 275
Size: 382 635 1451
Fragments arranged by size:
1451 1062 936 636 635 537 382 352
186 83 53
BsaWI w'CCGG_w
Cuts at: 2952 4746 5144 5418 5565 5976 2952
Size: 1794 398 274 147 411 3289
Fragments arranged by size:
3289 1794 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2729 2759 1178
Size: 30 165 30 1326 30 4732
Fragments arranged by size:
4732 1326 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2153 2516 2558 2600 2870 2978 3071 3113
Size: 967 363 42 42 270 108 93 42
Cuts at: 3113 3575 1186
Size: 462 3924
Fragments arranged by size:
3924 967 462 363 270 108 93 42
42 42
BseYI C'CCAG_C
Cuts at: 2729 3166 3345 5516 6012 2729
Size: 437 179 2171 496 3030
Fragments arranged by size:
3030 2171 496 437 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2414 2507 2807 3089 3392 1163
Size: 573 678 93 300 282 303 4084
Fragments arranged by size:
4084 678 573 303 300 282 93
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3763 4524 5552 1540
Size: 275 186 1762 761 1028 2301
Fragments arranged by size:
2301 1762 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2151 2196 2259 2420 2529 3278 3318 4374 4459
Size: 45 63 161 109 749 40 1056 85
Cuts at: 4459 5530 2151
Size: 1071 2934
Fragments arranged by size:
2934 1071 1056 749 161 109 85 63
45 40
BsmI GAATG_Cn'
Cuts at: 3824 3917 3824
Size: 93 6220
BsmBI CGTCTCn'nnnn_
Cuts at: 3254 3254
Size: 6313
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2151 2196 2259 2420 2529 3278 3318
Size: 399 361 45 63 161 109 749 40
Cuts at: 3318 3386 3611 3632 4374 4459 5530 1391
Size: 68 225 21 742 85 1071 2174
Fragments arranged by size:
2174 1071 749 742 399 361 225 161
109 85 68 63 45 40 21
BspEI T'CCGG_A
Cuts at: 5976 5976
Size: 6313
BspHI T'CATG_A
Cuts at: 2373 2655 4202 5932 2373
Size: 282 1547 1730 2754
Fragments arranged by size:
2754 1730 1547 282
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2112 2134 2628 3145 3187 3409 3668 844
Size: 1268 22 494 517 42 222 259 3489
Fragments arranged by size:
3489 1268 517 494 259 222 42 22
BsrBI CCG'CTC
Cuts at: 1394 1823 3073 3478 4200 1394
Size: 429 1250 405 722 3507
Fragments arranged by size:
3507 1250 722 429 405
BsrDI GCAATG_nn'
Cuts at: 65 1248 4808 4982 65
Size: 1183 3560 174 1396
Fragments arranged by size:
3560 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2216 2299 2513 3371 4957 5144 1700
Size: 110 406 83 214 858 1586 187 2869
Fragments arranged by size:
2869 1586 858 406 214 187 110 83
BsrGI T'GTAC_A
Cuts at: 96 2274 3018 96
Size: 2178 744 3391
Fragments arranged by size:
3391 2178 744
BssHII G'CGCG_C
Cuts at: 1925 3520 1925
Size: 1595 4718
BssSI C'ACGA_G
Cuts at: 4373 5385 4373
Size: 1012 5301
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2875 3424 794
Size: 2081 549 3683
Fragments arranged by size:
3683 2081 549
BstBI TT'CG_AA
Cuts at: 5117 5117
Size: 6313
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6313
BstXI CCAn_nnnn'nTGG
Cuts at: 2569 2569
Size: 6313
BstYI r'GATC_y
Cuts at: 1972 3643 4010 4395 4412 5864 5950 5962 6308
Size: 1671 367 385 17 1452 86 12 346
Cuts at: 6308 1972
Size: 1977
Fragments arranged by size:
1977 1671 1452 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 3527 3527
Size: 6313
BtgI C'CryG_G
Cuts at: 513 1065 2715 3228 3579 513
Size: 552 1650 513 351 3247
Fragments arranged by size:
3247 1650 552 513 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5574
Fragments arranged by size:
5574 683 56
BtsI GCAGTG_nn'
Cuts at: 2117 2993 3829 4623 4643 2117
Size: 876 836 794 20 3787
Fragments arranged by size:
3787 876 836 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6313
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6278
DraI TTT'AAA
Cuts at: 3617 3617
Size: 6313
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5320 809
Size: 507 4004 1802
Fragments arranged by size:
4004 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2218 2559 3225
Size: 54 1425 50 275 18 388 341 666
Cuts at: 3225 3760 4649 6278 8
Size: 535 889 1629 43
Fragments arranged by size:
1629 1425 889 666 535 388 341 275
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3760 1537
Size: 275 1948 4090
Fragments arranged by size:
4090 1948 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4243 1353
Size: 2890 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2918 3194 4900 5274 5420 387
Size: 1064 138 1329 276 1706 374 146 1280
Fragments arranged by size:
1706 1329 1280 1064 374 276 146 138
EcoICRI GAG'CTC
Cuts at: 2257 2418 2527 3276 3316 2257
Size: 161 109 749 40 5254
Fragments arranged by size:
5254 749 161 109 40
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2273 2648 2720 2759 2816 2873
Size: 393 434 207 375 72 39 57 57
Cuts at: 2873 3278 3356 4376 4973 5760 6028 1239
Size: 405 78 1020 597 787 268 1524
Fragments arranged by size:
1524 1020 787 597 434 405 393 375
268 207 78 72 57 57 39
EcoNI CCTnn'n_nnAGG
Cuts at: 2657 2774 2918 2930 2657
Size: 117 144 12 6040
Fragments arranged by size:
6040 144 117 12
EcoO109I rG'GnC_Cy
Cuts at: 2119 2398 2767 2923 3124 3306 3331 2119
Size: 279 369 156 201 182 25 5101
Fragments arranged by size:
5101 369 279 201 182 156 25
EcoRI G'AATT_C
Cuts at: 3622 4000 3622
Size: 378 5935
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3383 3383
Size: 6313
FspI TGC'GCA
Cuts at: 2468 2918 3149 4819 2468
Size: 450 231 1670 3962
Fragments arranged by size:
3962 1670 450 231
HaeII r_GCGC'y
Cuts at: 1678 2536 2598 2876 3487 5460 1678
Size: 858 62 278 611 1973 2531
Fragments arranged by size:
2531 1973 858 611 278 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4954 4986 5028 5060 1373
Size: 32 135 32 3382 32 42 32 2626
Fragments arranged by size:
3382 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3657 3903 669
Size: 988 281 1719 246 3079
Fragments arranged by size:
3079 1719 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6313
HpaI GTT'AAC
Cuts at: 3903 3903
Size: 6313
KasI G'GCGC_C
Cuts at: 2594 3483 2594
Size: 889 5424
KpnI G_GTAC'C
Cuts at: 3638 3638
Size: 6313
MfeI C'AATT_G
Cuts at: 3912 3912
Size: 6313
MluI A'CGCG_T
Cuts at: 2328 6189 2328
Size: 3861 2452
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1989 3303 5427 5611 1305
Size: 684 1314 2124 184 2007
Fragments arranged by size:
2124 2007 1314 684 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2561 10
Size: 54 1425 343 729 3762
Fragments arranged by size:
3762 1425 729 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2580 4271 4630 4789 518
Size: 648 1414 1691 359 159 2042
Fragments arranged by size:
2042 1691 1414 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2212 2272 2356 2626 2725 3193 3256 3456
Size: 606 60 84 270 99 468 63 200
Cuts at: 3456 3729 4406 5554 5799 1606
Size: 273 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 606 468 273 270 245
200 99 84 63 60
NaeI GCC'GGC
Cuts at: 2301 2515 3373 2301
Size: 214 858 5241
Fragments arranged by size:
5241 858 214
NarI GG'CG_CC
Cuts at: 2595 3484 2595
Size: 889 5424
NcoI C'CATG_G
Cuts at: 513 1065 2715 513
Size: 552 1650 4111
Fragments arranged by size:
4111 1650 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6313
NgoMIV G'CCGG_C
Cuts at: 2299 2513 3371 2299
Size: 214 858 5241
Fragments arranged by size:
5241 858 214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6313
NotI GC'GGCC_GC
Cuts at: 3760 3760
Size: 6313
NspI r_CATG'y
Cuts at: 1132 1229 3671 5216 1132
Size: 97 2442 1545 2229
Fragments arranged by size:
2442 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6313
PciI A'CATG_T
Cuts at: 5212 5212
Size: 6313
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2851 3334 1108
Size: 1743 483 4087
Fragments arranged by size:
4087 1743 483
PfoI T'CCnGG_A
Cuts at: 2043 2196 2808 2043
Size: 153 612 5548
Fragments arranged by size:
5548 612 153
PmeI GTTT'AAAC
Cuts at: 3617 3617
Size: 6313
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4382 4414 5895 5927 4382
Size: 32 1481 32 4768
Fragments arranged by size:
4768 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2119 2398 2923 3306 3331 2119
Size: 279 525 383 25 5101
Fragments arranged by size:
5101 525 383 279 25
PshAI GACnn'nnGTC
Cuts at: 1613 2369 3266 1613
Size: 756 897 4660
Fragments arranged by size:
4660 897 756
PsiI TTA'TAA
Cuts at: 3883 3883
Size: 6313
PstI C_TGCA'G
Cuts at: 830 2211 2385 2625 2757 2988 3051 3132 3192
Size: 1381 174 240 132 231 63 81 60
Cuts at: 3192 3198 3665 830
Size: 6 467 3478
Fragments arranged by size:
3478 1381 467 240 231 174 132 81
63 60 6
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6313
PvuII CAG'CTG
Cuts at: 2272 2356 2626 3193 3256 2272
Size: 84 270 567 63 5329
Fragments arranged by size:
5329 567 270 84 63
RsrII CG'GwC_CG
Cuts at: 2214 2214
Size: 6313
SacI G_AGCT'C
Cuts at: 2259 2420 2529 3278 3318 2259
Size: 161 109 749 40 5254
Fragments arranged by size:
5254 749 161 109 40
SalI G'TCGA_C
Cuts at: 1936 3655 1936
Size: 1719 4594
SbfI CC_TGCA'GG
Cuts at: 3132 3665 3132
Size: 533 5780
ScaI AGT'ACT
Cuts at: 4561 6273 4561
Size: 1712 4601
SexAI A'CCwGG_T
Cuts at: 2163 2163
Size: 6313
SfcI C'TryA_G
Cuts at: 826 1045 2207 2381 2621 2753 2984 3047 3128
Size: 219 1162 174 240 132 231 63 81
Cuts at: 3128 3188 3194 3661 4796 5477 5668 826
Size: 60 6 467 1135 681 191 1471
Fragments arranged by size:
1471 1162 1135 681 467 240 231 219
191 174 132 81 63 60 6
SfoI GGC'GCC
Cuts at: 2596 3485 2596
Size: 889 5424
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6313
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6313
SmaI CCC'GGG
Cuts at: 1872 2783 3122 3640 1872
Size: 911 339 518 4545
Fragments arranged by size:
4545 911 518 339
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2243 2495 2528 2642 4417 5318
Size: 197 926 300 252 33 114 1775 901
Cuts at: 5318 5580 820
Size: 262 1553
Fragments arranged by size:
1775 1553 926 901 300 262 252 197
114 33
SnaBI TAC'GTA
Cuts at: 493 3002 4104 493
Size: 2509 1102 2702
Fragments arranged by size:
2702 2509 1102
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6313
SphI G_CATG'C
Cuts at: 1132 3671 1132
Size: 2539 3774
SspI AAT'ATT
Cuts at: 5 52 4237 5
Size: 47 4185 2081
Fragments arranged by size:
4185 2081 47
StuI AGG'CCT
Cuts at: 1517 2346 2801 3447 1517
Size: 829 455 646 4383
Fragments arranged by size:
4383 829 646 455
StyI C'CwwG_G
Cuts at: 513 1065 2715 3752 513
Size: 552 1650 1037 3074
Fragments arranged by size:
3074 1650 1037 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2062 2177 3313 3359 4340 4357 4510
Size: 1255 482 115 1136 46 981 17 153
Cuts at: 4510 4695 5140 325
Size: 185 445 1498
Fragments arranged by size:
1498 1255 1136 981 482 445 185 153
115 46 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2274 3018 4559
Size: 274 80 33 51 1443 297 744 1541
Cuts at: 4559 6271 96
Size: 1712 138
Fragments arranged by size:
1712 1541 1443 744 297 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2173 2205 2729 2761 6273 6305 2173
Size: 32 524 32 3512 32 2181
Fragments arranged by size:
3512 2181 524 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6313
XbaI T'CTAG_A
Cuts at: 3649 3649
Size: 6313
XhoI C'TCGA_G
Cuts at: 1943 2243 1943
Size: 300 6013
XmaI C'CCGG_G
Cuts at: 1870 2781 3120 3638 1870
Size: 911 339 518 4545
Fragments arranged by size:
4545 911 518 339
XmnI GAAnn'nnTTC
Cuts at: 4442 4442
Size: 6313
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4121 276
Size: 53 83 186 936 2587 2468
Fragments arranged by size:
2587 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PstI PvuI PvuII RsrII SacI SalI
SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI
PacI PmlI PspOMI PspXI PsrI SacII SanDI SapI
SfiI SrfI SwaI XcmI