Restriction map for
pFN21AB9949
(Circular) (Six-base) MAPSORT of: pFN21AB9949.seq Check: 2785 from: 1 to: 6301
pFN21AB9949 6301 bp
With 182 enzymes: SgfI *
April 15, 2010 09:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3045 3045
Size: 6301
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2566 4111 278
Size: 53 83 186 936 1030 1545 2468
Fragments arranged by size:
2468 1545 1030 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3481 3644 1937
Size: 1544 163 4594
Fragments arranged by size:
4594 1544 163
Acc65I G'GTAC_C
Cuts at: 3385 3622 3385
Size: 237 6064
AclI AA'CG_TT
Cuts at: 1563 3436 4428 4801 1563
Size: 1873 992 373 3063
Fragments arranged by size:
3063 1873 992 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2280 2823 4364 5748 6016 1632
Size: 648 543 1541 1384 268 1917
Fragments arranged by size:
1917 1541 1384 648 543 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6104
AflIII A'CryG_T
Cuts at: 1203 5200 6177 1203
Size: 3997 977 1327
Fragments arranged by size:
3997 1327 977
AgeI A'CCGG_T
Cuts at: 5132 5132
Size: 6301
AhdI GACnn_n'nnGTC
Cuts at: 5030 5030
Size: 6301
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6301
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6301
AlwNI CAG_nnn'CTG
Cuts at: 2080 4044 5616 2080
Size: 1964 1572 2765
Fragments arranged by size:
2765 1964 1572
ApaI G_GGCC'C
Cuts at: 3520 3520
Size: 6301
ApaLI G'TGCA_C
Cuts at: 4358 5514 4358
Size: 1156 5145
ApoI r'AATT_y
Cuts at: 1493 3048 3161 3610 3836 3988 6097 1493
Size: 1555 113 449 226 152 2109 1697
Fragments arranged by size:
2109 1697 1555 449 226 152 113
AseI AT'TA_AT
Cuts at: 160 1032 2220 4855 160
Size: 872 1188 2635 1606
Fragments arranged by size:
2635 1606 1188 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
AvaI C'yCGr_G
Cuts at: 1870 1943 3626 1870
Size: 73 1683 4545
Fragments arranged by size:
4545 1683 73
BamHI G'GATC_C
Cuts at: 3631 3998 3631
Size: 367 5934
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3056 3212 3385 3622 5077
Size: 325 217 627 1269 156 173 237 1455
Cuts at: 5077 618
Size: 1842
Fragments arranged by size:
1842 1455 1269 627 325 237 217 173
156
BanII G_rGCy'C
Cuts at: 1391 2995 3520 3620 1391
Size: 1604 525 100 4072
Fragments arranged by size:
4072 1604 525 100
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3566 928
Size: 971 1667 3663
Fragments arranged by size:
3663 1667 971
BbvCI CC'TCA_GC
Cuts at: 2010 2010
Size: 6301
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4492 4526 1903
Size: 34 50 34 2471 34 3678
Fragments arranged by size:
3678 2471 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2158 4193 5403 2158
Size: 2035 1210 3056
Fragments arranged by size:
3056 2035 1210
BclI T'GATC_A
Cuts at: 1548 2505 1548
Size: 957 5344
BfrBI ATG'CAT
Cuts at: 2232 2232
Size: 6301
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4912 136
Size: 107 122 71 631 605 3240 1525
Fragments arranged by size:
3240 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6296 6296
Size: 6301
BlpI GC'TnA_GC
Cuts at: 3718 3718
Size: 6301
Bme1580I G_kGCm'C
Cuts at: 1790 2202 3195 3215 3520 4362 5518 1790
Size: 412 993 20 305 842 1156 2573
Fragments arranged by size:
2573 1156 993 842 412 305 20
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6301
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2112 4032 4990
Size: 416 53 83 399 24 695 1920 958
Cuts at: 4990 442
Size: 1753
Fragments arranged by size:
1920 1753 958 695 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6301
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2500 3517 4961 1239
Size: 1261 1017 1444 2579
Fragments arranged by size:
2579 1444 1261 1017
Bpu10I CC'TnA_GC
Cuts at: 2010 2152 2194 2010
Size: 142 42 6117
Fragments arranged by size:
6117 142 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2539 4426 5291 5589 2539
Size: 1887 865 298 3251
Fragments arranged by size:
3251 1887 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3081 4964 882
Size: 617 1582 1883 2219
Fragments arranged by size:
2219 1883 1582 617
BsaAI yAC'GTr
Cuts at: 493 1204 4092 493
Size: 711 2888 2702
Fragments arranged by size:
2888 2702 711
BsaBI GATnn'nnATC
Cuts at: 6295 6295
Size: 6301
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2563 4108 4490 5125
Size: 53 83 186 936 1030 1545 382 635
Cuts at: 5125 275
Size: 1451
Fragments arranged by size:
1545 1451 1030 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4734 5132 5406 5553 5964 4734
Size: 398 274 147 411 5071
Fragments arranged by size:
5071 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3336 3366 1178
Size: 30 165 30 1933 30 4113
Fragments arranged by size:
4113 1933 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6301
BseYI C'CCAG_C
Cuts at: 2352 3513 5504 6000 2352
Size: 1161 1991 496 2653
Fragments arranged by size:
2653 1991 1161 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3023 1163
Size: 573 1287 4441
Fragments arranged by size:
4441 1287 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3751 4512 5540 1540
Size: 275 186 1750 761 1028 2301
Fragments arranged by size:
2301 1750 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4362 4447 5518 4362
Size: 85 1071 5145
Fragments arranged by size:
5145 1071 85
BsmI GAATG_Cn'
Cuts at: 2454 3515 3812 3905 2454
Size: 1061 297 93 4850
Fragments arranged by size:
4850 1061 297 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2202 2995 3195 3215 3520 3620 4362
Size: 399 412 793 200 20 305 100 742
Cuts at: 4362 4447 5518 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 793 742 412 399 305 200
100 85 20
BspEI T'CCGG_A
Cuts at: 5964 5964
Size: 6301
BspHI T'CATG_A
Cuts at: 4190 5920 4190
Size: 1730 4571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3045 3656 844
Size: 2201 611 3489
Fragments arranged by size:
3489 2201 611
BsrBI CCG'CTC
Cuts at: 1394 1823 4188 1394
Size: 429 2365 3507
Fragments arranged by size:
3507 2365 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2245 3135 4796 4970 65
Size: 1183 997 890 1661 174 1396
Fragments arranged by size:
1661 1396 1183 997 890 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4945 5132 1700
Size: 110 3135 187 2869
Fragments arranged by size:
3135 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6301
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6301
BssSI C'ACGA_G
Cuts at: 2061 4361 5373 2061
Size: 2300 1012 2989
Fragments arranged by size:
2989 2300 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6301
BstBI TT'CG_AA
Cuts at: 5105 5105
Size: 6301
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6301
BstXI CCAn_nnnn'nTGG
Cuts at: 2945 2945
Size: 6301
BstYI r'GATC_y
Cuts at: 1972 2318 2948 3631 3998 4383 4400 5852 5938
Size: 346 630 683 367 385 17 1452 86
Cuts at: 5938 5950 6296 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 683 630 385 367 346 346
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5749
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5562
Fragments arranged by size:
5562 683 56
BtsI GCAGTG_nn'
Cuts at: 2414 3817 4611 4631 2414
Size: 1403 794 20 4084
Fragments arranged by size:
4084 1403 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6301
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2746 2781 529
Size: 35 2182 35 4049
Fragments arranged by size:
4049 2182 35 35
DraI TTT'AAA
Cuts at: 2181 3605 2181
Size: 1424 4877
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5308 809
Size: 507 3992 1802
Fragments arranged by size:
3992 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3443 3748 4637
Size: 54 1425 50 275 18 1613 305 889
Cuts at: 4637 6266 8
Size: 1629 43
Fragments arranged by size:
1629 1613 1425 889 305 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3748 1537
Size: 275 1936 4090
Fragments arranged by size:
4090 1936 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4231 1353
Size: 2878 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4888 5262 5408 387
Size: 1064 138 3299 374 146 1280
Fragments arranged by size:
3299 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2280 2500 2823 3517 4364 4961 5748
Size: 393 648 220 323 694 847 597 787
Cuts at: 5748 6016 1239
Size: 268 1524
Fragments arranged by size:
1524 847 787 694 648 597 393 323
268 220
EcoRI G'AATT_C
Cuts at: 3610 3988 3610
Size: 378 5923
FspI TGC'GCA
Cuts at: 4807 4807
Size: 6301
HaeII r_GCGC'y
Cuts at: 1678 5448 1678
Size: 3770 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3313 3345 4942 4974 5016
Size: 32 135 32 1741 32 1597 32 42
Cuts at: 5016 5048 1373
Size: 32 2626
Fragments arranged by size:
2626 1741 1597 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3645 3891 669
Size: 988 281 1707 246 3079
Fragments arranged by size:
3079 1707 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6301
HpaI GTT'AAC
Cuts at: 3891 3891
Size: 6301
KpnI G_GTAC'C
Cuts at: 3389 3626 3389
Size: 237 6064
MfeI C'AATT_G
Cuts at: 3075 3365 3447 3900 3075
Size: 290 82 453 5476
Fragments arranged by size:
5476 453 290 82
MluI A'CGCG_T
Cuts at: 6177 6177
Size: 6301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2145 2360 2590 5415 5599 1305
Size: 840 215 230 2825 184 2007
Fragments arranged by size:
2825 2007 840 230 215 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4479
Fragments arranged by size:
4479 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2105 2480 2943 4259 4618 4777 518
Size: 648 939 375 463 1316 359 159 2042
Fragments arranged by size:
2042 1316 939 648 463 375 359 159
MspA1I CmG'CkG
Cuts at: 1606 2520 2814 3017 3717 4394 5542 5787 1606
Size: 914 294 203 700 677 1148 245 2120
Fragments arranged by size:
2120 1148 914 700 677 294 245 203
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5749
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6301
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6301
NotI GC'GGCC_GC
Cuts at: 3748 3748
Size: 6301
NsiI A_TGCA'T
Cuts at: 2234 2234
Size: 6301
NspI r_CATG'y
Cuts at: 1132 1229 2232 3659 5204 1132
Size: 97 1003 1427 1545 2229
Fragments arranged by size:
2229 1545 1427 1003 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6301
PciI A'CATG_T
Cuts at: 5200 5200
Size: 6301
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6301
PfoI T'CCnGG_A
Cuts at: 2906 2906
Size: 6301
PmeI GTTT'AAAC
Cuts at: 3605 3605
Size: 6301
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4370 4402 5883 5915 4370
Size: 32 1481 32 4756
Fragments arranged by size:
4756 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6301
PsiI TTA'TAA
Cuts at: 3871 3871
Size: 6301
PspOMI G'GGCC_C
Cuts at: 3516 3516
Size: 6301
PstI C_TGCA'G
Cuts at: 830 3653 830
Size: 2823 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6301
PvuII CAG'CTG
Cuts at: 2520 2814 3017 2520
Size: 294 203 5804
Fragments arranged by size:
5804 294 203
SalI G'TCGA_C
Cuts at: 1936 3643 1936
Size: 1707 4594
SbfI CC_TGCA'GG
Cuts at: 3653 3653
Size: 6301
ScaI AGT'ACT
Cuts at: 4549 6261 4549
Size: 1712 4589
SfcI C'TryA_G
Cuts at: 826 1045 3295 3649 4784 5465 5656 826
Size: 219 2250 354 1135 681 191 1471
Fragments arranged by size:
2250 1471 1135 681 354 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6301
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6301
SmaI CCC'GGG
Cuts at: 1872 3628 1872
Size: 1756 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2554 4405 5306 5568 820
Size: 197 926 611 1851 901 262 1553
Fragments arranged by size:
1851 1553 926 901 611 262 197
SnaBI TAC'GTA
Cuts at: 493 4092 493
Size: 3599 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6301
SphI G_CATG'C
Cuts at: 1132 3659 1132
Size: 2527 3774
SspI AAT'ATT
Cuts at: 5 52 4225 5
Size: 47 4173 2081
Fragments arranged by size:
4173 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6301
StyI C'CwwG_G
Cuts at: 513 1065 3061 3740 513
Size: 552 1996 679 3074
Fragments arranged by size:
3074 1996 679 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3074 4328 4345 4498 4683 5128 325
Size: 1255 1494 1254 17 153 185 445 1498
Fragments arranged by size:
1498 1494 1255 1254 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4547 6259 96
Size: 274 80 33 51 1443 2570 1712 138
Fragments arranged by size:
2570 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3336 3368 6261 6293 3336
Size: 32 2893 32 3344
Fragments arranged by size:
3344 2893 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6301
XbaI T'CTAG_A
Cuts at: 3637 3637
Size: 6301
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3223 3223
Size: 6301
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6301
XmaI C'CCGG_G
Cuts at: 1870 3626 1870
Size: 1756 4545
XmnI GAAnn'nnTTC
Cuts at: 2789 4430 2789
Size: 1641 4660
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2564 4109 276
Size: 53 83 186 936 1030 1545 2468
Fragments arranged by size:
2468 1545 1030 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI