Restriction map for
pFN21AB9947
(Circular) (Six-base) MAPSORT of: pFN21AB9947.seq Check: 43 from: 1 to: 6298
pFN21AB9947 6298 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4108 278
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3641 1937
Size: 1704 4594
Acc65I G'GTAC_C
Cuts at: 3619 3619
Size: 6298
AclI AA'CG_TT
Cuts at: 1563 4425 4798 1563
Size: 2862 373 3063
Fragments arranged by size:
3063 2862 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2426 2711 3054 4361 5745 6013 1632
Size: 794 285 343 1307 1384 268 1917
Fragments arranged by size:
1917 1384 1307 794 343 285 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6101
AflIII A'CryG_T
Cuts at: 1203 2091 5197 6174 1203
Size: 888 3106 977 1327
Fragments arranged by size:
3106 1327 977 888
AgeI A'CCGG_T
Cuts at: 5129 5129
Size: 6298
AhdI GACnn_n'nnGTC
Cuts at: 3384 5027 3384
Size: 1643 4655
AleI CACnn'nnGTG
Cuts at: 1166 3242 1166
Size: 2076 4222
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2586 3162 1184
Size: 1402 576 4320
Fragments arranged by size:
4320 1402 576
AlwNI CAG_nnn'CTG
Cuts at: 2056 2689 2995 3142 3164 4041 5613 2056
Size: 633 306 147 22 877 1572 2741
Fragments arranged by size:
2741 1572 877 633 306 147 22
ApaLI G'TGCA_C
Cuts at: 4355 5511 4355
Size: 1156 5142
ApoI r'AATT_y
Cuts at: 1493 3607 3833 3985 6094 1493
Size: 2114 226 152 2109 1697
Fragments arranged by size:
2114 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4852 160
Size: 872 3820 1606
Fragments arranged by size:
3820 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
AvaI C'yCGr_G
Cuts at: 1870 1943 3027 3501 3623 1870
Size: 73 1084 474 122 4545
Fragments arranged by size:
4545 1084 474 122 73
BamHI G'GATC_C
Cuts at: 3628 3995 3628
Size: 367 5931
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2570 3208 3248 3549 3619
Size: 325 217 627 783 638 40 301 70
Cuts at: 3619 5074 618
Size: 1455 1842
Fragments arranged by size:
1842 1455 783 638 627 325 301 217
70 40
BanII G_rGCy'C
Cuts at: 1391 2373 3617 1391
Size: 982 1244 4072
Fragments arranged by size:
4072 1244 982
BbeI G_GCGC'C
Cuts at: 3212 3553 3212
Size: 341 5957
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2914 928
Size: 971 1015 4312
Fragments arranged by size:
4312 1015 971
BbvCI CC'TCA_GC
Cuts at: 3453 3453
Size: 6298
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4489 4523 1903
Size: 34 2552 34 3678
Fragments arranged by size:
3678 2552 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4190 5400 4190
Size: 1210 5088
BclI T'GATC_A
Cuts at: 1548 3006 1548
Size: 1458 4840
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3155 3257 4909
Size: 107 122 71 631 605 1483 102 1652
Cuts at: 4909 136
Size: 1525
Fragments arranged by size:
1652 1525 1483 631 605 122 107 102
71
BglII A'GATC_T
Cuts at: 2523 6293 2523
Size: 3770 2528
BlpI GC'TnA_GC
Cuts at: 3715 3715
Size: 6298
Bme1580I G_kGCm'C
Cuts at: 1790 3349 4359 5515 1790
Size: 1559 1010 1156 2573
Fragments arranged by size:
2573 1559 1156 1010
BmgBI CAC'GTC
Cuts at: 1318 3460 1318
Size: 2142 4156
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2376 3428 4029
Size: 416 53 83 399 24 959 1052 601
Cuts at: 4029 4987 442
Size: 958 1753
Fragments arranged by size:
1753 1052 959 958 601 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6298
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2438 2438
Size: 6298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2105 2537 2876 3017 3111 3140 4958 1239
Size: 866 432 339 141 94 29 1818 2579
Fragments arranged by size:
2579 1818 866 432 339 141 94 29
Bpu10I CC'TnA_GC
Cuts at: 3453 3453
Size: 6298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4423 5288 5586 4423
Size: 865 298 5135
Fragments arranged by size:
5135 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2255 4961 882
Size: 617 756 2706 2219
Fragments arranged by size:
2706 2219 756 617
BsaAI yAC'GTr
Cuts at: 493 1204 2530 3344 4089 493
Size: 711 1326 814 745 2702
Fragments arranged by size:
2702 1326 814 745 711
BsaBI GATnn'nnATC
Cuts at: 6292 6292
Size: 6298
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3209 3550 4105 4487
Size: 53 83 186 936 1676 341 555 382
Cuts at: 4487 5122 275
Size: 635 1451
Fragments arranged by size:
1676 1451 936 635 555 382 341 186
83 53
BsaWI w'CCGG_w
Cuts at: 3542 4731 5129 5403 5550 5961 3542
Size: 1189 398 274 147 411 3879
Fragments arranged by size:
3879 1189 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2078 2108 3513 3543 1178
Size: 30 165 30 675 30 1405 30 3933
Fragments arranged by size:
3933 1405 675 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6298
BseYI C'CCAG_C
Cuts at: 2049 2161 2963 5501 5997 2049
Size: 112 802 2538 496 2350
Fragments arranged by size:
2538 2350 802 496 112
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2042 1163
Size: 573 306 5419
Fragments arranged by size:
5419 573 306
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3748 4509 5537 1540
Size: 275 186 1747 761 1028 2301
Fragments arranged by size:
2301 1747 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2091 4359 4444 5515 2091
Size: 2268 85 1071 2874
Fragments arranged by size:
2874 2268 1071 85
BsmI GAATG_Cn'
Cuts at: 3809 3902 3809
Size: 93 6205
BsmBI CGTCTCn'nnnn_
Cuts at: 2174 2174
Size: 6298
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2091 2373 3349 3617 4359 4444 5515
Size: 399 301 282 976 268 742 85 1071
Cuts at: 5515 1391
Size: 2174
Fragments arranged by size:
2174 1071 976 742 399 301 282 268
85
BspEI T'CCGG_A
Cuts at: 5961 5961
Size: 6298
BspHI T'CATG_A
Cuts at: 2245 2824 4187 5917 2245
Size: 579 1363 1730 2626
Fragments arranged by size:
2626 1730 1363 579
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3300 3653 844
Size: 2456 353 3489
Fragments arranged by size:
3489 2456 353
BsrBI CCG'CTC
Cuts at: 1394 1823 4185 1394
Size: 429 2362 3507
Fragments arranged by size:
3507 2362 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3354 4793 4967 65
Size: 1183 2106 1439 174 1396
Fragments arranged by size:
2106 1439 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2319 3595 4942 5129 1700
Size: 110 509 1276 1347 187 2869
Fragments arranged by size:
2869 1347 1276 509 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6298
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6298
BssSI C'ACGA_G
Cuts at: 2883 4358 5370 2883
Size: 1475 1012 3811
Fragments arranged by size:
3811 1475 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3164 794
Size: 2370 3928
BstBI TT'CG_AA
Cuts at: 5102 5102
Size: 6298
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6298
BstXI CCAn_nnnn'nTGG
Cuts at: 3244 3244
Size: 6298
BstYI r'GATC_y
Cuts at: 1972 2358 2523 2686 3628 3995 4380 4397 5849
Size: 386 165 163 942 367 385 17 1452
Cuts at: 5849 5935 5947 6293 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 942 386 385 367 346 165
163 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2067 2286 3243 3252 3403 513
Size: 552 1002 219 957 9 151 3408
Fragments arranged by size:
3408 1002 957 552 219 151 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5559
Fragments arranged by size:
5559 683 56
BtsI GCAGTG_nn'
Cuts at: 2388 3046 3283 3814 4608 4628 2388
Size: 658 237 531 794 20 4058
Fragments arranged by size:
4058 794 658 531 237 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6298
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6263
DraI TTT'AAA
Cuts at: 3602 3602
Size: 6298
DraIII CAC_nnn'GTG
Cuts at: 3325 3325
Size: 6298
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5305 809
Size: 507 3989 1802
Fragments arranged by size:
3989 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3048 3327 3745
Size: 54 1425 50 275 18 1218 279 418
Cuts at: 3745 4634 6263 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1218 889 418 279 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3745 1537
Size: 275 1933 4090
Fragments arranged by size:
4090 1933 275
EarI CTCTTCn'nnn_
Cuts at: 1353 4228 1353
Size: 2875 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3188 4885 5259 5405 387
Size: 1064 138 1599 1697 374 146 1280
Fragments arranged by size:
1697 1599 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2105 2426 2537 2711 2876 3017 3054
Size: 393 473 321 111 174 165 141 37
Cuts at: 3054 3111 3140 4361 4958 5745 6013 1239
Size: 57 29 1221 597 787 268 1524
Fragments arranged by size:
1524 1221 787 597 473 393 321 268
174 165 141 111 57 37 29
EcoO109I rG'GnC_Cy
Cuts at: 2351 2773 3430 2351
Size: 422 657 5219
Fragments arranged by size:
5219 657 422
EcoRI G'AATT_C
Cuts at: 3607 3985 3607
Size: 378 5920
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2316 2316
Size: 6298
FspI TGC'GCA
Cuts at: 2279 3131 4804 2279
Size: 852 1673 3773
Fragments arranged by size:
3773 1673 852
FspAI rTGC'GCAy
Cuts at: 2279 3131 2279
Size: 852 5446
HaeII r_GCGC'y
Cuts at: 1678 3212 3553 5445 1678
Size: 1534 341 1892 2531
Fragments arranged by size:
2531 1892 1534 341
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2343 2375 3355 3370 3387
Size: 32 135 32 771 32 980 15 17
Cuts at: 3387 3402 3513 3545 4939 4971 5013 5045 1373
Size: 15 111 32 1394 32 42 32 2626
Fragments arranged by size:
2626 1394 980 771 135 111 42 32
32 32 32 32 32 17 15 15
HincII GTy'rAC
Cuts at: 669 1657 1938 3642 3888 669
Size: 988 281 1704 246 3079
Fragments arranged by size:
3079 1704 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2293 748
Size: 1545 4753
HpaI GTT'AAC
Cuts at: 3888 3888
Size: 6298
KasI G'GCGC_C
Cuts at: 3208 3549 3208
Size: 341 5957
KpnI G_GTAC'C
Cuts at: 3623 3623
Size: 6298
MfeI C'AATT_G
Cuts at: 3897 3897
Size: 6298
MluI A'CGCG_T
Cuts at: 6174 6174
Size: 6298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2649 5412 5596 1305
Size: 1344 2763 184 2007
Fragments arranged by size:
2763 2007 1344 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3050 3329 10
Size: 54 1425 343 1218 279 2979
Fragments arranged by size:
2979 1425 1218 343 279 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3242 3359 4256 4615 4774 518
Size: 648 2076 117 897 359 159 2042
Fragments arranged by size:
2076 2042 897 648 359 159 117
MspA1I CmG'CkG
Cuts at: 1606 2056 2385 3714 4391 5539 5784 1606
Size: 450 329 1329 677 1148 245 2120
Fragments arranged by size:
2120 1329 1148 677 450 329 245
NarI GG'CG_CC
Cuts at: 3209 3550 3209
Size: 341 5957
NcoI C'CATG_G
Cuts at: 513 1065 2067 513
Size: 552 1002 4744
Fragments arranged by size:
4744 1002 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6298
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6298
NotI GC'GGCC_GC
Cuts at: 3745 3745
Size: 6298
NspI r_CATG'y
Cuts at: 1132 1229 2095 3136 3656 5201 1132
Size: 97 866 1041 520 1545 2229
Fragments arranged by size:
2229 1545 1041 866 520 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6298
PciI A'CATG_T
Cuts at: 2091 5197 2091
Size: 3106 3192
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6298
PfoI T'CCnGG_A
Cuts at: 2082 2682 2853 3060 3438 2082
Size: 600 171 207 378 4942
Fragments arranged by size:
4942 600 378 207 171
PmeI GTTT'AAAC
Cuts at: 3602 3602
Size: 6298
PmlI CAC'GTG
Cuts at: 3344 3344
Size: 6298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4367 4399 5880 5912 4367
Size: 32 1481 32 4753
Fragments arranged by size:
4753 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2351 2773 3430 2351
Size: 422 657 5219
Fragments arranged by size:
5219 657 422
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6298
PsiI TTA'TAA
Cuts at: 3868 3868
Size: 6298
PstI C_TGCA'G
Cuts at: 830 2395 2914 3278 3650 830
Size: 1565 519 364 372 3478
Fragments arranged by size:
3478 1565 519 372 364
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6298
PvuII CAG'CTG
Cuts at: 2056 2056
Size: 6298
SalI G'TCGA_C
Cuts at: 1936 3640 1936
Size: 1704 4594
SbfI CC_TGCA'GG
Cuts at: 3650 3650
Size: 6298
ScaI AGT'ACT
Cuts at: 4546 6258 4546
Size: 1712 4586
SexAI A'CCwGG_T
Cuts at: 3321 3321
Size: 6298
SfcI C'TryA_G
Cuts at: 826 1045 2391 2910 3274 3646 4781 5462 5653
Size: 219 1346 519 364 372 1135 681 191
Cuts at: 5653 826
Size: 1471
Fragments arranged by size:
1471 1346 1135 681 519 372 364 219
191
SfoI GGC'GCC
Cuts at: 3210 3551 3210
Size: 341 5957
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6298
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6298
SmaI CCC'GGG
Cuts at: 1872 3625 1872
Size: 1753 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4402 5303 5565 820
Size: 197 926 2459 901 262 1553
Fragments arranged by size:
2459 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4089 493
Size: 3596 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6298
SphI G_CATG'C
Cuts at: 1132 3136 3656 1132
Size: 2004 520 3774
Fragments arranged by size:
3774 2004 520
SspI AAT'ATT
Cuts at: 5 52 4222 5
Size: 47 4170 2081
Fragments arranged by size:
4170 2081 47
StuI AGG'CCT
Cuts at: 1517 3260 3446 1517
Size: 1743 186 4369
Fragments arranged by size:
4369 1743 186
StyI C'CwwG_G
Cuts at: 513 1065 2067 2610 3737 513
Size: 552 1002 543 1127 3074
Fragments arranged by size:
3074 1127 1002 552 543
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2134 3236 4325 4342 4495 4680 5125
Size: 1255 554 1102 1089 17 153 185 445
Cuts at: 5125 325
Size: 1498
Fragments arranged by size:
1498 1255 1102 1089 554 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4544 6256 96
Size: 274 80 33 51 1443 2567 1712 138
Fragments arranged by size:
2567 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2078 2110 2199 2231 6258 6290 2078
Size: 32 89 32 4027 32 2086
Fragments arranged by size:
4027 2086 89 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3434 1316
Size: 2118 4180
XbaI T'CTAG_A
Cuts at: 2338 3634 2338
Size: 1296 5002
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3058 3401 3058
Size: 343 5955
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6298
XmaI C'CCGG_G
Cuts at: 1870 3623 1870
Size: 1753 4545
XmnI GAAnn'nnTTC
Cuts at: 2921 4427 2921
Size: 1506 4792
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4106 276
Size: 53 83 186 936 2572 2468
Fragments arranged by size:
2572 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI
BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI NaeI
NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII
SacI SacII SanDI SapI SfiI SrfI SwaI