Restriction map for
pFN21AB9944
(Circular) (Six-base) MAPSORT of: pFN21AB9944.seq Check: 8475 from: 1 to: 6661
pFN21AB9944 6661 bp
With 182 enzymes: SgfI *
March 28, 2012 12:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2411 3434 2411
Size: 1023 5638
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2513 2832 4471 278
Size: 53 83 186 936 977 319 1639 2468
Fragments arranged by size:
2468 1639 977 936 319 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4004 1937
Size: 2067 4594
Acc65I G'GTAC_C
Cuts at: 3982 3982
Size: 6661
AclI AA'CG_TT
Cuts at: 1563 3960 4788 5161 1563
Size: 2397 828 373 3063
Fragments arranged by size:
3063 2397 828 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2285 3668 4724 6108 6376 1632
Size: 653 1383 1056 1384 268 1917
Fragments arranged by size:
1917 1384 1383 1056 653 268
AfeI AGC'GCT
Cuts at: 2972 2972
Size: 6661
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6464
AflIII A'CryG_T
Cuts at: 1203 2934 3390 3687 5560 6537 1203
Size: 1731 456 297 1873 977 1327
Fragments arranged by size:
1873 1731 1327 977 456 297
AgeI A'CCGG_T
Cuts at: 5492 5492
Size: 6661
AhdI GACnn_n'nnGTC
Cuts at: 3217 5390 3217
Size: 2173 4488
AleI CACnn'nnGTG
Cuts at: 1166 2474 3899 1166
Size: 1308 1425 3928
Fragments arranged by size:
3928 1425 1308
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6661
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3747 3779 3747
Size: 32 6629
AlwNI CAG_nnn'CTG
Cuts at: 2843 2896 3337 4404 5976 2843
Size: 53 441 1067 1572 3528
Fragments arranged by size:
3528 1572 1067 441 53
ApaLI G'TGCA_C
Cuts at: 3421 4718 5874 3421
Size: 1297 1156 4208
Fragments arranged by size:
4208 1297 1156
ApoI r'AATT_y
Cuts at: 1493 3923 3970 4196 4348 6457 1493
Size: 2430 47 226 152 2109 1697
Fragments arranged by size:
2430 2109 1697 226 152 47
AseI AT'TA_AT
Cuts at: 160 1032 5215 160
Size: 872 4183 1606
Fragments arranged by size:
4183 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
AvaI C'yCGr_G
Cuts at: 1870 1943 2015 2445 2956 3308 3986 1870
Size: 73 72 430 511 352 678 4545
Fragments arranged by size:
4545 678 511 430 352 73 72
BamHI G'GATC_C
Cuts at: 3991 4358 3991
Size: 367 6294
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3982 5437 618
Size: 325 217 627 2195 1455 1842
Fragments arranged by size:
2195 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2733 3328 3585 3980 1391
Size: 1342 595 257 395 4072
Fragments arranged by size:
4072 1342 595 395 257
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5690
BbvCI CC'TCA_GC
Cuts at: 2662 3489 2662
Size: 827 5834
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4852 4886 1903
Size: 34 2915 34 3678
Fragments arranged by size:
3678 2915 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4553 5763 4553
Size: 1210 5451
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6661
BfrBI ATG'CAT
Cuts at: 2880 2880
Size: 6661
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3332 5272 136
Size: 107 122 71 631 605 1660 1940 1525
Fragments arranged by size:
1940 1660 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6656 6656
Size: 6661
BlpI GC'TnA_GC
Cuts at: 4078 4078
Size: 6661
Bme1580I G_kGCm'C
Cuts at: 1790 3398 3425 4722 5878 1790
Size: 1608 27 1297 1156 2573
Fragments arranged by size:
2573 1608 1297 1156 27
BmgBI CAC'GTC
Cuts at: 1318 2095 3357 1318
Size: 777 1262 4622
Fragments arranged by size:
4622 1262 777
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2310 3541 3886
Size: 416 53 83 399 24 893 1231 345
Cuts at: 3886 4392 5350 442
Size: 506 958 1753
Fragments arranged by size:
1753 1231 958 893 506 416 399 345
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6661
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 6661
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2196 2563 2635 3215 3698 5321 1239
Size: 957 367 72 580 483 1623 2579
Fragments arranged by size:
2579 1623 957 580 483 367 72
Bpu10I CC'TnA_GC
Cuts at: 2662 3489 2662
Size: 827 5834
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3111 4786 5651 5949 3111
Size: 1675 865 298 3823
Fragments arranged by size:
3823 1675 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2176 5324 882
Size: 617 677 3148 2219
Fragments arranged by size:
3148 2219 677 617
BsaAI yAC'GTr
Cuts at: 493 1204 3391 4452 493
Size: 711 2187 1061 2702
Fragments arranged by size:
2702 2187 1061 711
BsaBI GATnn'nnATC
Cuts at: 6655 6655
Size: 6661
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2510 2829 4468 4850
Size: 53 83 186 936 977 319 1639 382
Cuts at: 4850 5485 275
Size: 635 1451
Fragments arranged by size:
1639 1451 977 936 635 382 319 186
83 53
BsaWI w'CCGG_w
Cuts at: 2338 3450 5094 5492 5766 5913 6324 2338
Size: 1112 1644 398 274 147 411 2675
Fragments arranged by size:
2675 1644 1112 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2122 2152 2960 2990 3747
Size: 30 165 30 719 30 808 30 757
Cuts at: 3747 3777 1178
Size: 30 4062
Fragments arranged by size:
4062 808 757 719 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2093 2766 2879 1186
Size: 907 673 113 4968
Fragments arranged by size:
4968 907 673 113
BseYI C'CCAG_C
Cuts at: 2156 2843 3301 5864 6360 2156
Size: 687 458 2563 496 2457
Fragments arranged by size:
2563 2457 687 496 458
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2439 2445 2885 3523 1163
Size: 573 703 6 440 638 4301
Fragments arranged by size:
4301 703 638 573 440 6
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3451 4111 4872 5900 1540
Size: 275 186 1450 660 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 660 275 186
BsiHKAI G_wGCw'C
Cuts at: 3425 3585 4722 4807 5878 3425
Size: 160 1137 85 1071 4208
Fragments arranged by size:
4208 1137 1071 160 85
BsmI GAATG_Cn'
Cuts at: 4172 4265 4172
Size: 93 6568
BsmBI CGTCTCn'nnnn_
Cuts at: 2125 2861 2125
Size: 736 5925
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2733 3328 3398 3425 3585 3980 4722
Size: 399 943 595 70 27 160 395 742
Cuts at: 4722 4807 5878 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 943 742 595 399 395 160
85 70 27
BspEI T'CCGG_A
Cuts at: 6324 6324
Size: 6661
BspHI T'CATG_A
Cuts at: 4550 6280 4550
Size: 1730 4931
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2411 2904 2999 3198 3434 4016 844
Size: 1567 493 95 199 236 582 3489
Fragments arranged by size:
3489 1567 582 493 236 199 95
BsrBI CCG'CTC
Cuts at: 1394 1823 2011 3010 4548 1394
Size: 429 188 999 1538 3507
Fragments arranged by size:
3507 1538 999 429 188
BsrDI GCAATG_nn'
Cuts at: 65 1248 5156 5330 65
Size: 1183 3908 174 1396
Fragments arranged by size:
3908 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3252 3548 5305 5492 1700
Size: 110 1442 296 1757 187 2869
Fragments arranged by size:
2869 1757 1442 296 187 110
BsrGI T'GTAC_A
Cuts at: 96 2322 96
Size: 2226 4435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6661
BssSI C'ACGA_G
Cuts at: 2822 3041 3704 4721 5733 2822
Size: 219 663 1017 1012 3750
Fragments arranged by size:
3750 1017 1012 663 219
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2886 3429 794
Size: 2092 543 4026
Fragments arranged by size:
4026 2092 543
BstBI TT'CG_AA
Cuts at: 5465 5465
Size: 6661
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6661
BstYI r'GATC_y
Cuts at: 1972 3012 3991 4358 4743 4760 6212 6298 6310
Size: 1040 979 367 385 17 1452 86 12
Cuts at: 6310 6656 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1040 979 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2102 2413 2102
Size: 311 6350
BtgI C'CryG_G
Cuts at: 513 1065 2004 2544 3003 513
Size: 552 939 540 459 4171
Fragments arranged by size:
4171 939 552 540 459
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5922
Fragments arranged by size:
5922 683 56
BtsI GCAGTG_nn'
Cuts at: 3088 3263 3295 3500 4177 4971 4991 3088
Size: 175 32 205 677 794 20 4758
Fragments arranged by size:
4758 794 677 205 175 32 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6661
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6626
DraI TTT'AAA
Cuts at: 3965 3965
Size: 6661
DraIII CAC_nnn'GTG
Cuts at: 2350 2350
Size: 6661
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3479 5668 809
Size: 507 2163 2189 1802
Fragments arranged by size:
2189 2163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3849 4108 4997
Size: 54 1425 50 275 18 2019 259 889
Cuts at: 4997 6626 8
Size: 1629 43
Fragments arranged by size:
2019 1629 1425 889 275 259 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4108 1537
Size: 275 2296 4090
Fragments arranged by size:
4090 2296 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2525 2545 4591 1353
Size: 1172 20 2046 3423
Fragments arranged by size:
3423 2046 1172 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2143 5248 5622 5768 387
Size: 1064 138 554 3105 374 146 1280
Fragments arranged by size:
3105 1280 1064 554 374 146 138
EcoICRI GAG'CTC
Cuts at: 3583 3583
Size: 6661
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2196 2285 2563 2635 3215 3668 3698
Size: 393 564 89 278 72 580 453 30
Cuts at: 3698 4724 5321 6108 6376 1239
Size: 1026 597 787 268 1524
Fragments arranged by size:
1524 1026 787 597 580 564 453 393
278 268 89 72 30
EcoNI CCTnn'n_nnAGG
Cuts at: 3402 3402
Size: 6661
EcoO109I rG'GnC_Cy
Cuts at: 2449 2761 2893 3187 3334 3472 2449
Size: 312 132 294 147 138 5638
Fragments arranged by size:
5638 312 294 147 138 132
EcoRI G'AATT_C
Cuts at: 3970 4348 3970
Size: 378 6283
FspI TGC'GCA
Cuts at: 3788 5167 3788
Size: 1379 5282
HaeII r_GCGC'y
Cuts at: 1678 2974 5808 1678
Size: 1296 2834 2531
Fragments arranged by size:
2834 2531 1296
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2192 2224 2633 2665 2960
Size: 32 135 32 620 32 409 32 295
Cuts at: 2960 2992 3203 3235 3335 3367 5302 5334 5376
Size: 32 211 32 100 32 1935 32 42
Cuts at: 5376 5408 1373
Size: 32 2626
Fragments arranged by size:
2626 1935 620 409 295 211 135 100
42 32 32 32 32 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3211 3693 4005 4251 669
Size: 988 281 1273 482 312 246 3079
Fragments arranged by size:
3079 1273 988 482 312 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6661
HpaI GTT'AAC
Cuts at: 4251 4251
Size: 6661
KpnI G_GTAC'C
Cuts at: 3986 3986
Size: 6661
MfeI C'AATT_G
Cuts at: 4260 4260
Size: 6661
MluI A'CGCG_T
Cuts at: 6537 6537
Size: 6661
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2682 5775 5959 1305
Size: 1377 3093 184 2007
Fragments arranged by size:
3093 2007 1377 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4839
Fragments arranged by size:
4839 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3899 3936 4619 4978 5137 518
Size: 648 1308 1425 37 683 359 159 2042
Fragments arranged by size:
2042 1425 1308 683 648 359 159 37
MspA1I CmG'CkG
Cuts at: 1606 2840 2997 3718 4077 4754 5902 6147 1606
Size: 1234 157 721 359 677 1148 245 2120
Fragments arranged by size:
2120 1234 1148 721 677 359 245 157
NaeI GCC'GGC
Cuts at: 3254 3254
Size: 6661
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 5170
Fragments arranged by size:
5170 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6661
NgoMIV G'CCGG_C
Cuts at: 3252 3252
Size: 6661
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6661
NotI GC'GGCC_GC
Cuts at: 4108 4108
Size: 6661
NsiI A_TGCA'T
Cuts at: 2882 2882
Size: 6661
NspI r_CATG'y
Cuts at: 1132 1229 2289 2880 2932 3136 3691 4019 5564
Size: 97 1060 591 52 204 555 328 1545
Cuts at: 5564 1132
Size: 2229
Fragments arranged by size:
2229 1545 1060 591 555 328 204 97
52
PasI CC'CwG_GG
Cuts at: 1348 2049 3337 1348
Size: 701 1288 4672
Fragments arranged by size:
4672 1288 701
PciI A'CATG_T
Cuts at: 3687 5560 3687
Size: 1873 4788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6661
PfoI T'CCnGG_A
Cuts at: 3381 3381
Size: 6661
PmeI GTTT'AAAC
Cuts at: 3965 3965
Size: 6661
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3747 3779 4730 4762 6243 6275 3747
Size: 32 951 32 1481 32 4133
Fragments arranged by size:
4133 1481 951 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2449 2761 2893 3187 3472 2449
Size: 312 132 294 285 5638
Fragments arranged by size:
5638 312 294 285 132
PshAI GACnn'nnGTC
Cuts at: 1613 3075 3377 3600 1613
Size: 1462 302 223 4674
Fragments arranged by size:
4674 1462 302 223
PsiI TTA'TAA
Cuts at: 4231 4231
Size: 6661
PstI C_TGCA'G
Cuts at: 830 2563 3141 3513 4013 830
Size: 1733 578 372 500 3478
Fragments arranged by size:
3478 1733 578 500 372
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6661
RsrII CG'GwC_CG
Cuts at: 3546 3546
Size: 6661
SacI G_AGCT'C
Cuts at: 3585 3585
Size: 6661
SalI G'TCGA_C
Cuts at: 1936 4003 1936
Size: 2067 4594
SanDI GG'GwC_CC
Cuts at: 2449 2449
Size: 6661
SbfI CC_TGCA'GG
Cuts at: 2563 4013 2563
Size: 1450 5211
ScaI AGT'ACT
Cuts at: 4909 6621 4909
Size: 1712 4949
SfcI C'TryA_G
Cuts at: 826 1045 2185 2559 3137 3509 4009 5144 5825
Size: 219 1140 374 578 372 500 1135 681
Cuts at: 5825 6016 826
Size: 191 1471
Fragments arranged by size:
1471 1140 1135 681 578 500 374 372
219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6661
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6661
SmaI CCC'GGG
Cuts at: 1872 2447 3988 1872
Size: 575 1541 4545
Fragments arranged by size:
4545 1541 575
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3126 4765 5666 5928 820
Size: 197 926 1183 1639 901 262 1553
Fragments arranged by size:
1639 1553 1183 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4452 493
Size: 3959 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6661
SphI G_CATG'C
Cuts at: 1132 2932 4019 1132
Size: 1800 1087 3774
Fragments arranged by size:
3774 1800 1087
SspI AAT'ATT
Cuts at: 5 52 4585 5
Size: 47 4533 2081
Fragments arranged by size:
4533 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6661
StyI C'CwwG_G
Cuts at: 513 1065 2004 4100 513
Size: 552 939 2096 3074
Fragments arranged by size:
3074 2096 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3581 3710 4688 4705 4858 5043 5488
Size: 1255 2001 129 978 17 153 185 445
Cuts at: 5488 325
Size: 1498
Fragments arranged by size:
2001 1498 1255 978 445 185 153 129
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2322 3267 3528
Size: 274 80 33 51 1443 345 945 261
Cuts at: 3528 4907 6619 96
Size: 1379 1712 138
Fragments arranged by size:
1712 1443 1379 945 345 274 261 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3377 3409 6621 6653 3377
Size: 32 3212 32 3385
Fragments arranged by size:
3385 3212 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 2686 1316
Size: 1370 5291
XbaI T'CTAG_A
Cuts at: 3997 3997
Size: 6661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2164 2164
Size: 6661
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6661
XmaI C'CCGG_G
Cuts at: 1870 2445 3986 1870
Size: 575 1541 4545
Fragments arranged by size:
4545 1541 575
XmnI GAAnn'nnTTC
Cuts at: 4790 4790
Size: 6661
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2511 2830 4469 276
Size: 53 83 186 936 977 319 1639 2468
Fragments arranged by size:
2468 1639 977 936 319 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PstI PvuI RsrII SacI SalI
SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
ApaI AscI AvrII BaeI BbeI BsiWI BstXI BstZ17I
EcoRV FalI FseI FspAI KasI NarI NruI PacI
PmlI PspOMI PspXI PsrI PvuII SacII SapI SexAI
SfiI SfoI SrfI SwaI