Restriction map for
pFN21AB9943
(Circular) (Six-base) MAPSORT of: pFN21AB9943.seq Check: 5770 from: 1 to: 6196
pFN21AB9943 6196 bp
With 182 enzymes: SgfI *
April 15, 2010 09:37 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4006 278
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2667 3539 1937
Size: 730 872 4594
Fragments arranged by size:
4594 872 730
Acc65I G'GTAC_C
Cuts at: 3517 3517
Size: 6196
AclI AA'CG_TT
Cuts at: 1563 4323 4696 1563
Size: 2760 373 3063
Fragments arranged by size:
3063 2760 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2127 2153 2284 2402 2708 4259 5643 5911
Size: 495 26 131 118 306 1551 1384 268
Cuts at: 5911 1632
Size: 1917
Fragments arranged by size:
1917 1551 1384 495 306 268 131 118
26
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5999
AflIII A'CryG_T
Cuts at: 1203 5095 6072 1203
Size: 3892 977 1327
Fragments arranged by size:
3892 1327 977
AgeI A'CCGG_T
Cuts at: 5027 5027
Size: 6196
AhdI GACnn_n'nnGTC
Cuts at: 4925 4925
Size: 6196
AleI CACnn'nnGTG
Cuts at: 1166 3002 1166
Size: 1836 4360
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6196
AlwNI CAG_nnn'CTG
Cuts at: 3428 3939 5511 3428
Size: 511 1572 4113
Fragments arranged by size:
4113 1572 511
ApaLI G'TGCA_C
Cuts at: 4253 5409 4253
Size: 1156 5040
ApoI r'AATT_y
Cuts at: 1493 2151 2210 2231 2717 2967 3242 3315 3505
Size: 658 59 21 486 250 275 73 190
Cuts at: 3505 3731 3883 5992 1493
Size: 226 152 2109 1697
Fragments arranged by size:
2109 1697 658 486 275 250 226 190
152 73 59 21
AseI AT'TA_AT
Cuts at: 160 1032 4750 160
Size: 872 3718 1606
Fragments arranged by size:
3718 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
AvaI C'yCGr_G
Cuts at: 1870 1943 3521 1870
Size: 73 1578 4545
Fragments arranged by size:
4545 1578 73
BamHI G'GATC_C
Cuts at: 3526 3893 3526
Size: 367 5829
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2747 3517 4972 618
Size: 325 217 627 960 770 1455 1842
Fragments arranged by size:
1842 1455 960 770 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3515 1391
Size: 2124 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2564 928
Size: 971 665 4560
Fragments arranged by size:
4560 971 665
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4387 4421 1903
Size: 34 2450 34 3678
Fragments arranged by size:
3678 2450 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4088 5298 4088
Size: 1210 4986
BclI T'GATC_A
Cuts at: 1548 2764 1548
Size: 1216 4980
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4807 136
Size: 107 122 71 631 605 3135 1525
Fragments arranged by size:
3135 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6191 6191
Size: 6196
BlpI GC'TnA_GC
Cuts at: 3613 3613
Size: 6196
Bme1580I G_kGCm'C
Cuts at: 1790 4257 5413 1790
Size: 2467 1156 2573
Fragments arranged by size:
2573 2467 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6196
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3124 3927 4885
Size: 416 53 83 399 24 1707 803 958
Cuts at: 4885 442
Size: 1753
Fragments arranged by size:
1753 1707 958 803 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3490 4856 1239
Size: 2251 1366 2579
Fragments arranged by size:
2579 2251 1366
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2635 4321 5186 5484 2635
Size: 1686 865 298 3347
Fragments arranged by size:
3347 1686 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4859 882
Size: 617 3360 2219
Fragments arranged by size:
3360 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3987 493
Size: 711 2783 2702
Fragments arranged by size:
2783 2702 711
BsaBI GATnn'nnATC
Cuts at: 2763 6190 2763
Size: 3427 2769
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2105 4003 4385 5020
Size: 53 83 186 936 572 1898 382 635
Cuts at: 5020 275
Size: 1451
Fragments arranged by size:
1898 1451 936 635 572 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4629 5027 5301 5448 5859 4629
Size: 398 274 147 411 4966
Fragments arranged by size:
4966 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5971
Fragments arranged by size:
5971 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6196
BseYI C'CCAG_C
Cuts at: 3219 5399 5895 3219
Size: 2180 496 3520
Fragments arranged by size:
3520 2180 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5623
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3646 4407 5435 1540
Size: 275 186 1645 761 1028 2301
Fragments arranged by size:
2301 1645 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4257 4342 5413 4257
Size: 85 1071 5040
Fragments arranged by size:
5040 1071 85
BsmI GAATG_Cn'
Cuts at: 3707 3800 3707
Size: 93 6103
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3515 4257 4342 5413 1391
Size: 399 1725 742 85 1071 2174
Fragments arranged by size:
2174 1725 1071 742 399 85
BspEI T'CCGG_A
Cuts at: 5859 5859
Size: 6196
BspHI T'CATG_A
Cuts at: 4085 5815 4085
Size: 1730 4466
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3551 844
Size: 2707 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4083 1394
Size: 429 2260 3507
Fragments arranged by size:
3507 2260 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4691 4865 65
Size: 1183 3443 174 1396
Fragments arranged by size:
3443 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2090 4840 5027 1700
Size: 110 280 2750 187 2869
Fragments arranged by size:
2869 2750 280 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6196
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6196
BssSI C'ACGA_G
Cuts at: 4256 5268 4256
Size: 1012 5184
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2317 794
Size: 1523 4673
BstBI TT'CG_AA
Cuts at: 5000 5000
Size: 6196
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6196
BstYI r'GATC_y
Cuts at: 1972 3358 3526 3893 4278 4295 5747 5833 5845
Size: 1386 168 367 385 17 1452 86 12
Cuts at: 5845 6191 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1386 385 367 346 168 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2046 513
Size: 552 939 42 4663
Fragments arranged by size:
4663 939 552 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5457
Fragments arranged by size:
5457 683 56
BtsI GCAGTG_nn'
Cuts at: 3712 4506 4526 3712
Size: 794 20 5382
Fragments arranged by size:
5382 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6196
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6161
DraI TTT'AAA
Cuts at: 2236 2512 3500 2236
Size: 276 988 4932
Fragments arranged by size:
4932 988 276
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5203 809
Size: 507 3887 1802
Fragments arranged by size:
3887 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2040 2079
Size: 54 1425 50 275 18 177 33 39
Cuts at: 2079 2388 3643 4532 6161 8
Size: 309 1255 889 1629 43
Fragments arranged by size:
1629 1425 1255 889 309 275 177 54
50 43 39 33 18
EagI C'GGCC_G
Cuts at: 1537 1812 3643 1537
Size: 275 1831 4090
Fragments arranged by size:
4090 1831 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2511 2532 2547 2691 2694 3141 3484 4126
Size: 1158 21 15 144 3 447 343 642
Cuts at: 4126 1353
Size: 3423
Fragments arranged by size:
3423 1158 642 447 343 144 21 15
3
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4783 5157 5303 387
Size: 1064 138 3194 374 146 1280
Fragments arranged by size:
3194 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2127 2153 2284 2402 2708 3490 4259
Size: 393 495 26 131 118 306 782 769
Cuts at: 4259 4856 5643 5911 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 782 769 597 495 393 306
268 131 118 26
EcoRI G'AATT_C
Cuts at: 2151 3505 3883 2151
Size: 1354 378 4464
Fragments arranged by size:
4464 1354 378
FspI TGC'GCA
Cuts at: 4702 4702
Size: 6196
HaeII r_GCGC'y
Cuts at: 1678 2114 3184 5343 1678
Size: 436 1070 2159 2531
Fragments arranged by size:
2531 2159 1070 436
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2358 2390 4837 4869 4911
Size: 32 135 32 786 32 2447 32 42
Cuts at: 4911 4943 1373
Size: 32 2626
Fragments arranged by size:
2626 2447 786 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3540 3786 669
Size: 988 281 1602 246 3079
Fragments arranged by size:
3079 1602 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2634 3081 748
Size: 1886 447 3863
Fragments arranged by size:
3863 1886 447
HpaI GTT'AAC
Cuts at: 3786 3786
Size: 6196
KpnI G_GTAC'C
Cuts at: 3521 3521
Size: 6196
MfeI C'AATT_G
Cuts at: 2384 3795 2384
Size: 1411 4785
MluI A'CGCG_T
Cuts at: 6072 6072
Size: 6196
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3488 5310 5494 1305
Size: 2183 1822 184 2007
Fragments arranged by size:
2183 2007 1822 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2390 10
Size: 54 1425 343 558 3816
Fragments arranged by size:
3816 1425 558 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2403 3002 4154 4513 4672 518
Size: 648 1237 599 1152 359 159 2042
Fragments arranged by size:
2042 1237 1152 648 599 359 159
MspA1I CmG'CkG
Cuts at: 1606 2048 3612 4289 5437 5682 1606
Size: 442 1564 677 1148 245 2120
Fragments arranged by size:
2120 1564 1148 677 442 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4705
Fragments arranged by size:
4705 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6196
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6196
NotI GC'GGCC_GC
Cuts at: 3643 3643
Size: 6196
NspI r_CATG'y
Cuts at: 1132 1229 3554 5099 1132
Size: 97 2325 1545 2229
Fragments arranged by size:
2325 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6196
PciI A'CATG_T
Cuts at: 5095 5095
Size: 6196
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3390 1108
Size: 2282 3914
PfoI T'CCnGG_A
Cuts at: 2842 2842
Size: 6196
PmeI GTTT'AAAC
Cuts at: 3500 3500
Size: 6196
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4265 4297 5778 5810 4265
Size: 32 1481 32 4651
Fragments arranged by size:
4651 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6196
PsiI TTA'TAA
Cuts at: 3766 3766
Size: 6196
PstI C_TGCA'G
Cuts at: 830 3548 830
Size: 2718 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6196
SacII CC_GC'GG
Cuts at: 2049 2049
Size: 6196
SalI G'TCGA_C
Cuts at: 1936 3538 1936
Size: 1602 4594
SapI GCTCTTCn'nnn_
Cuts at: 2511 2532 2511
Size: 21 6175
SbfI CC_TGCA'GG
Cuts at: 3548 3548
Size: 6196
ScaI AGT'ACT
Cuts at: 2140 4444 6156 2140
Size: 2304 1712 2180
Fragments arranged by size:
2304 2180 1712
SfcI C'TryA_G
Cuts at: 826 1045 2664 3544 4679 5360 5551 826
Size: 219 1619 880 1135 681 191 1471
Fragments arranged by size:
1619 1471 1135 880 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6196
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6196
SmaI CCC'GGG
Cuts at: 1872 3523 1872
Size: 1651 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2650 4300 5201 5463 820
Size: 197 926 707 1650 901 262 1553
Fragments arranged by size:
1650 1553 926 901 707 262 197
SnaBI TAC'GTA
Cuts at: 493 3987 493
Size: 3494 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6196
SphI G_CATG'C
Cuts at: 1132 3554 1132
Size: 2422 3774
SspI AAT'ATT
Cuts at: 5 52 4120 5
Size: 47 4068 2081
Fragments arranged by size:
4068 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6196
StyI C'CwwG_G
Cuts at: 513 1065 2004 3635 513
Size: 552 939 1631 3074
Fragments arranged by size:
3074 1631 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4223 4240 4393 4578 5023 325
Size: 1255 2643 17 153 185 445 1498
Fragments arranged by size:
2643 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2138 4442 6154
Size: 274 80 33 51 1443 161 2304 1712
Cuts at: 6154 96
Size: 138
Fragments arranged by size:
2304 1712 1443 274 161 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6156 6188 6156
Size: 32 6164
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6196
XbaI T'CTAG_A
Cuts at: 2830 2902 3532 2830
Size: 72 630 5494
Fragments arranged by size:
5494 630 72
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6196
XmaI C'CCGG_G
Cuts at: 1870 3521 1870
Size: 1651 4545
XmnI GAAnn'nnTTC
Cuts at: 2636 2826 2838 4325 2636
Size: 190 12 1487 4507
Fragments arranged by size:
4507 1487 190 12
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4004 276
Size: 53 83 186 936 2470 2468
Fragments arranged by size:
2470 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PshAI PsiI PstI PvuI SacII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI
SanDI SexAI SfiI SfoI SrfI SwaI XcmI