Restriction map for
pFN21AB9940
(Circular) (Six-base) MAPSORT of: pFN21AB9940.seq Check: 9892 from: 1 to: 6676
pFN21AB9940 6676 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4486 278
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4019 1937
Size: 2082 4594
Acc65I G'GTAC_C
Cuts at: 3162 3997 3162
Size: 835 5841
AclI AA'CG_TT
Cuts at: 1563 4803 5176 1563
Size: 3240 373 3063
Fragments arranged by size:
3240 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2825 2871 4739 6123 6391 1632
Size: 1193 46 1868 1384 268 1917
Fragments arranged by size:
1917 1868 1384 1193 268 46
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6479
AflIII A'CryG_T
Cuts at: 1203 2941 3153 5575 6552 1203
Size: 1738 212 2422 977 1327
Fragments arranged by size:
2422 1738 1327 977 212
AgeI A'CCGG_T
Cuts at: 5507 5507
Size: 6676
AhdI GACnn_n'nnGTC
Cuts at: 5405 5405
Size: 6676
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6676
AlwNI CAG_nnn'CTG
Cuts at: 3233 4419 5991 3233
Size: 1186 1572 3918
Fragments arranged by size:
3918 1572 1186
ApaI G_GGCC'C
Cuts at: 2869 2869
Size: 6676
ApaLI G'TGCA_C
Cuts at: 2505 3426 4733 5889 2505
Size: 921 1307 1156 3292
Fragments arranged by size:
3292 1307 1156 921
ApoI r'AATT_y
Cuts at: 1493 3132 3194 3268 3366 3848 3985 4211 4363
Size: 1639 62 74 98 482 137 226 152
Cuts at: 4363 6472 1493
Size: 2109 1697
Fragments arranged by size:
2109 1697 1639 482 226 152 137 98
74 62
AseI AT'TA_AT
Cuts at: 160 1032 5230 160
Size: 872 4198 1606
Fragments arranged by size:
4198 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
AvaI C'yCGr_G
Cuts at: 1870 1943 4001 1870
Size: 73 2058 4545
Fragments arranged by size:
4545 2058 73
AvrII C'CTAG_G
Cuts at: 3322 3322
Size: 6676
BamHI G'GATC_C
Cuts at: 2290 4006 4373 2290
Size: 1716 367 4593
Fragments arranged by size:
4593 1716 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3162 3997 5452 618
Size: 325 217 627 1375 835 1455 1842
Fragments arranged by size:
1842 1455 1375 835 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2045 2869 3839 3995 1391
Size: 654 824 970 156 4072
Fragments arranged by size:
4072 970 824 654 156
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2859 2977 3001 928
Size: 971 960 118 24 4603
Fragments arranged by size:
4603 971 960 118 24
BbvCI CC'TCA_GC
Cuts at: 3544 3544
Size: 6676
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4867 4901 1903
Size: 34 2930 34 3678
Fragments arranged by size:
3678 2930 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2180 4568 5778 2180
Size: 2388 1210 3078
Fragments arranged by size:
3078 2388 1210
BclI T'GATC_A
Cuts at: 1548 2577 3720 1548
Size: 1029 1143 4504
Fragments arranged by size:
4504 1143 1029
BfrBI ATG'CAT
Cuts at: 3503 3503
Size: 6676
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5287 136
Size: 107 122 71 631 605 3615 1525
Fragments arranged by size:
3615 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6671 6671
Size: 6676
BlpI GC'TnA_GC
Cuts at: 4093 4093
Size: 6676
Bme1580I G_kGCm'C
Cuts at: 1790 2509 2869 3430 4737 5893 1790
Size: 719 360 561 1307 1156 2573
Fragments arranged by size:
2573 1307 1156 719 561 360
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6676
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4407 5365 442
Size: 416 53 83 399 24 2990 958 1753
Fragments arranged by size:
2990 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6676
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 5336 1239
Size: 848 3249 2579
Fragments arranged by size:
3249 2579 848
Bpu10I CC'TnA_GC
Cuts at: 2832 3544 2832
Size: 712 5964
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4801 5666 5964 4801
Size: 865 298 5513
Fragments arranged by size:
5513 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2814 5339 882
Size: 617 1315 2525 2219
Fragments arranged by size:
2525 2219 1315 617
BsaAI yAC'GTr
Cuts at: 493 1204 2971 4467 493
Size: 711 1767 1496 2702
Fragments arranged by size:
2702 1767 1496 711
BsaBI GATnn'nnATC
Cuts at: 3185 6670 3185
Size: 3485 3191
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3924 4483 4865 5500
Size: 53 83 186 936 2391 559 382 635
Cuts at: 5500 275
Size: 1451
Fragments arranged by size:
2391 1451 936 635 559 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2316 5109 5507 5781 5928 6339 2316
Size: 2793 398 274 147 411 2653
Fragments arranged by size:
2793 2653 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2443 2473 2812 2842 3222
Size: 30 165 30 1040 30 339 30 380
Cuts at: 3222 3252 1178
Size: 30 4602
Fragments arranged by size:
4602 1040 380 339 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2861 1186
Size: 831 844 5001
Fragments arranged by size:
5001 844 831
BseYI C'CCAG_C
Cuts at: 2265 3665 5879 6375 2265
Size: 1400 2214 496 2566
Fragments arranged by size:
2566 2214 1400 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2065 2184 3523 1163
Size: 573 329 119 1339 4316
Fragments arranged by size:
4316 1339 573 329 119
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4126 4887 5915 1540
Size: 275 186 2125 761 1028 2301
Fragments arranged by size:
2301 2125 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2045 2509 2536 3430 4737 4822 5893 2045
Size: 464 27 894 1307 85 1071 2828
Fragments arranged by size:
2828 1307 1071 894 464 85 27
BsmI GAATG_Cn'
Cuts at: 3505 3583 4187 4280 3505
Size: 78 604 93 5901
Fragments arranged by size:
5901 604 93 78
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2045 2509 2536 2869 3430 3839 3995
Size: 399 255 464 27 333 561 409 156
Cuts at: 3995 4737 4822 5893 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 561 464 409 399 333
255 156 85 27
BspEI T'CCGG_A
Cuts at: 6339 6339
Size: 6676
BspHI T'CATG_A
Cuts at: 3063 4565 6295 3063
Size: 1502 1730 3444
Fragments arranged by size:
3444 1730 1502
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4031 844
Size: 3187 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4563 1394
Size: 429 2740 3507
Fragments arranged by size:
3507 2740 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3754 3906 5171 5345 65
Size: 1183 2506 152 1265 174 1396
Fragments arranged by size:
2506 1396 1265 1183 174 152
BsrFI r'CCGG_y
Cuts at: 1700 1810 3089 5320 5507 1700
Size: 110 1279 2231 187 2869
Fragments arranged by size:
2869 2231 1279 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6676
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6676
BssSI C'ACGA_G
Cuts at: 4736 5748 4736
Size: 1012 5664
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2162 3456 3554 3971 794
Size: 1368 1294 98 417 3499
Fragments arranged by size:
3499 1368 1294 417 98
BstBI TT'CG_AA
Cuts at: 5480 5480
Size: 6676
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6676
BstXI CCAn_nnnn'nTGG
Cuts at: 2975 2975
Size: 6676
BstYI r'GATC_y
Cuts at: 1972 2290 2730 3341 4006 4373 4758 4775 6227
Size: 318 440 611 665 367 385 17 1452
Cuts at: 6227 6313 6325 6671 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 665 611 440 385 367 346
318 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2093 513
Size: 552 939 89 5096
Fragments arranged by size:
5096 939 552 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2510 2941 487
Size: 683 56 1284 431 4222
Fragments arranged by size:
4222 1284 683 431 56
BtsI GCAGTG_nn'
Cuts at: 2999 4192 4986 5006 2999
Size: 1193 794 20 4669
Fragments arranged by size:
4669 1193 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6676
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6641
DraI TTT'AAA
Cuts at: 3492 3980 3492
Size: 488 6188
DraIII CAC_nnn'GTG
Cuts at: 2342 3434 2342
Size: 1092 5584
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2600 5683 809
Size: 507 1284 3083 1802
Fragments arranged by size:
3083 1802 1284 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4123 5012 6641
Size: 54 1425 50 275 18 2293 889 1629
Cuts at: 6641 8
Size: 43
Fragments arranged by size:
2293 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4123 1537
Size: 275 2311 4090
Fragments arranged by size:
4090 2311 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2111 2120 4606 1353
Size: 758 9 2486 3423
Fragments arranged by size:
3423 2486 758 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2011 2020 5263 5637 5783 387
Size: 1064 138 422 9 3243 374 146 1280
Fragments arranged by size:
3243 1280 1064 422 374 146 138 9
EcoICRI GAG'CTC
Cuts at: 2043 2043
Size: 6676
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2825 2871 4739 5336 6123 6391
Size: 393 455 738 46 1868 597 787 268
Cuts at: 6391 1239
Size: 1524
Fragments arranged by size:
1868 1524 787 738 597 455 393 268
46
EcoNI CCTnn'n_nnAGG
Cuts at: 3632 3632
Size: 6676
EcoO109I rG'GnC_Cy
Cuts at: 2572 2866 2572
Size: 294 6382
EcoRI G'AATT_C
Cuts at: 3848 3985 4363 3848
Size: 137 378 6161
Fragments arranged by size:
6161 378 137
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2876 3574 2876
Size: 698 5978
FspI TGC'GCA
Cuts at: 2936 5182 2936
Size: 2246 4430
HaeII r_GCGC'y
Cuts at: 1678 5823 1678
Size: 4145 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5317 5349 5391 5423 1373
Size: 32 135 32 3745 32 42 32 2626
Fragments arranged by size:
3745 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4020 4266 669
Size: 988 281 2082 246 3079
Fragments arranged by size:
3079 2082 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6676
HpaI GTT'AAC
Cuts at: 4266 4266
Size: 6676
KpnI G_GTAC'C
Cuts at: 3166 4001 3166
Size: 835 5841
MfeI C'AATT_G
Cuts at: 4275 4275
Size: 6676
MluI A'CGCG_T
Cuts at: 6552 6552
Size: 6676
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2211 3037 5790 5974 1305
Size: 906 826 2753 184 2007
Fragments arranged by size:
2753 2007 906 826 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4854
Fragments arranged by size:
4854 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2126 2529 3569 4634 4993 5152 518
Size: 648 960 403 1040 1065 359 159 2042
Fragments arranged by size:
2042 1065 1040 960 648 403 359 159
MspA1I CmG'CkG
Cuts at: 1606 2065 2269 4092 4769 5917 6162 1606
Size: 459 204 1823 677 1148 245 2120
Fragments arranged by size:
2120 1823 1148 677 459 245 204
NcoI C'CATG_G
Cuts at: 513 1065 2004 2093 513
Size: 552 939 89 5096
Fragments arranged by size:
5096 939 552 89
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6676
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6676
NotI GC'GGCC_GC
Cuts at: 4123 4123
Size: 6676
NsiI A_TGCA'T
Cuts at: 3505 3505
Size: 6676
NspI r_CATG'y
Cuts at: 1132 1229 3157 3210 4034 5579 1132
Size: 97 1928 53 824 1545 2229
Fragments arranged by size:
2229 1928 1545 824 97 53
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6676
PciI A'CATG_T
Cuts at: 3153 5575 3153
Size: 2422 4254
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2249 3172 1108
Size: 1141 923 4612
Fragments arranged by size:
4612 1141 923
PmeI GTTT'AAAC
Cuts at: 3980 3980
Size: 6676
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4745 4777 6258 6290 4745
Size: 32 1481 32 5131
Fragments arranged by size:
5131 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2179 1613
Size: 566 6110
PsiI TTA'TAA
Cuts at: 4246 4246
Size: 6676
PspOMI G'GGCC_C
Cuts at: 2865 2865
Size: 6676
PstI C_TGCA'G
Cuts at: 830 2018 3150 3653 4028 830
Size: 1188 1132 503 375 3478
Fragments arranged by size:
3478 1188 1132 503 375
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6676
SacI G_AGCT'C
Cuts at: 2045 2045
Size: 6676
SalI G'TCGA_C
Cuts at: 1936 4018 1936
Size: 2082 4594
SapI GCTCTTCn'nnn_
Cuts at: 2111 2111
Size: 6676
SbfI CC_TGCA'GG
Cuts at: 4028 4028
Size: 6676
ScaI AGT'ACT
Cuts at: 2721 4924 6636 2721
Size: 2203 1712 2761
Fragments arranged by size:
2761 2203 1712
SfcI C'TryA_G
Cuts at: 826 1045 2014 2331 2892 3146 3649 4024 5159
Size: 219 969 317 561 254 503 375 1135
Cuts at: 5159 5840 6031 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 969 681 561 503 375 317
254 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6676
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6676
SmaI CCC'GGG
Cuts at: 1872 4003 1872
Size: 2131 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4780 5681 5943 820
Size: 197 926 2837 901 262 1553
Fragments arranged by size:
2837 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4467 493
Size: 3974 2702
SpeI A'CTAG_T
Cuts at: 152 2176 2671 152
Size: 2024 495 4157
Fragments arranged by size:
4157 2024 495
SphI G_CATG'C
Cuts at: 1132 4034 1132
Size: 2902 3774
SspI AAT'ATT
Cuts at: 5 52 4600 5
Size: 47 4548 2081
Fragments arranged by size:
4548 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6676
StyI C'CwwG_G
Cuts at: 513 1065 2004 2093 2357 3322 3628 4115 513
Size: 552 939 89 264 965 306 487 3074
Fragments arranged by size:
3074 965 939 552 487 306 264 89
SwaI ATTT'AAAT
Cuts at: 3492 3492
Size: 6676
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2279 3040 3697 4703 4720 4873 5058
Size: 1255 699 761 657 1006 17 153 185
Cuts at: 5058 5503 325
Size: 445 1498
Fragments arranged by size:
1498 1255 1006 761 699 657 445 185
153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2719 2776 3054
Size: 274 80 33 51 1443 742 57 278
Cuts at: 3054 3150 4922 6634 96
Size: 96 1772 1712 138
Fragments arranged by size:
1772 1712 1443 742 278 274 138 96
80 57 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2443 2475 6636 6668 2443
Size: 32 4161 32 2451
Fragments arranged by size:
4161 2451 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6676
XbaI T'CTAG_A
Cuts at: 4012 4012
Size: 6676
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2689 2689
Size: 6676
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6676
XmaI C'CCGG_G
Cuts at: 1870 4001 1870
Size: 2131 4545
XmnI GAAnn'nnTTC
Cuts at: 3198 4805 3198
Size: 1607 5069
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4484 276
Size: 53 83 186 936 2950 2468
Fragments arranged by size:
2950 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NsiI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BplI BsiWI
BsmBI BstZ17I Bsu36I EcoRV FseI FspAI KasI NaeI
NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspXI
PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI
SrfI