Restriction map for
pFN21AB9937
(Circular) (Six-base) MAPSORT of: pFN21AB9937.seq Check: 1069 from: 1 to: 6271
pFN21AB9937 6271 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2787 4081 278
Size: 53 83 186 936 1251 1294 2468
Fragments arranged by size:
2468 1294 1251 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3614 1937
Size: 1677 4594
Acc65I G'GTAC_C
Cuts at: 2736 2828 3592 2736
Size: 92 764 5415
Fragments arranged by size:
5415 764 92
AclI AA'CG_TT
Cuts at: 1563 4398 4771 1563
Size: 2835 373 3063
Fragments arranged by size:
3063 2835 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2726 4334 5718 5986 1632
Size: 1094 1608 1384 268 1917
Fragments arranged by size:
1917 1608 1384 1094 268
AfeI AGC'GCT
Cuts at: 2032 2969 3027 3260 2032
Size: 937 58 233 5043
Fragments arranged by size:
5043 937 233 58
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6074
AflIII A'CryG_T
Cuts at: 1203 5170 6147 1203
Size: 3967 977 1327
Fragments arranged by size:
3967 1327 977
AgeI A'CCGG_T
Cuts at: 5102 5102
Size: 6271
AhdI GACnn_n'nnGTC
Cuts at: 5000 5000
Size: 6271
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6271
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6271
AlwNI CAG_nnn'CTG
Cuts at: 3523 4014 5586 3523
Size: 491 1572 4208
Fragments arranged by size:
4208 1572 491
ApaLI G'TGCA_C
Cuts at: 4328 5484 4328
Size: 1156 5115
ApoI r'AATT_y
Cuts at: 1493 2371 2702 3580 3806 3958 6067 1493
Size: 878 331 878 226 152 2109 1697
Fragments arranged by size:
2109 1697 878 878 331 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4825 160
Size: 872 3793 1606
Fragments arranged by size:
3793 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
AvaI C'yCGr_G
Cuts at: 1870 1943 2105 3365 3378 3596 1870
Size: 73 162 1260 13 218 4545
Fragments arranged by size:
4545 1260 218 162 73 13
BamHI G'GATC_C
Cuts at: 2232 3601 3968 2232
Size: 1369 367 4535
Fragments arranged by size:
4535 1369 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2150 2736 2828 3592 5047
Size: 325 217 627 363 586 92 764 1455
Cuts at: 5047 618
Size: 1842
Fragments arranged by size:
1842 1455 764 627 586 363 325 217
92
BanII G_rGCy'C
Cuts at: 1391 3074 3189 3590 1391
Size: 1683 115 401 4072
Fragments arranged by size:
4072 1683 401 115
BbeI G_GCGC'C
Cuts at: 2154 2154
Size: 6271
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3254 928
Size: 971 1355 3945
Fragments arranged by size:
3945 1355 971
BbvCI CC'TCA_GC
Cuts at: 3117 3117
Size: 6271
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4462 4496 1903
Size: 34 2525 34 3678
Fragments arranged by size:
3678 2525 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4163 5373 4163
Size: 1210 5061
BclI T'GATC_A
Cuts at: 1548 3483 1548
Size: 1935 4336
BfrBI ATG'CAT
Cuts at: 2486 2486
Size: 6271
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4882 136
Size: 107 122 71 631 605 3210 1525
Fragments arranged by size:
3210 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6266 6266
Size: 6271
BlpI GC'TnA_GC
Cuts at: 3183 3688 3183
Size: 505 5766
Bme1580I G_kGCm'C
Cuts at: 1790 3103 4332 5488 1790
Size: 1313 1229 1156 2573
Fragments arranged by size:
2573 1313 1229 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6271
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3034 3544 4002
Size: 416 53 83 399 24 1617 510 458
Cuts at: 4002 4960 442
Size: 958 1753
Fragments arranged by size:
1753 1617 958 510 458 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 2333 3026 1056
Size: 1277 693 4301
Fragments arranged by size:
4301 1277 693
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2064 2623 2064
Size: 559 5712
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2087 2927 4931 1239
Size: 848 840 2004 2579
Fragments arranged by size:
2579 2004 848 840
Bpu10I CC'TnA_GC
Cuts at: 3117 3117
Size: 6271
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2843 3515 4396 5261 5559 2843
Size: 672 881 865 298 3555
Fragments arranged by size:
3555 881 865 672 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2858 4934 882
Size: 617 1359 2076 2219
Fragments arranged by size:
2219 2076 1359 617
BsaAI yAC'GTr
Cuts at: 493 1204 2819 3481 4062 493
Size: 711 1615 662 581 2702
Fragments arranged by size:
2702 1615 711 662 581
BsaBI GATnn'nnATC
Cuts at: 6265 6265
Size: 6271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2151 2784 4078
Size: 53 83 186 936 476 142 633 1294
Cuts at: 4078 4460 5095 275
Size: 382 635 1451
Fragments arranged by size:
1451 1294 936 635 633 476 382 186
142 83 53
BsaWI w'CCGG_w
Cuts at: 4704 5102 5376 5523 5934 4704
Size: 398 274 147 411 5041
Fragments arranged by size:
5041 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2060 2090 2201 2231 2939
Size: 30 165 30 657 30 111 30 708
Cuts at: 2939 2969 1178
Size: 30 4480
Fragments arranged by size:
4480 708 657 165 111 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2346 2414 2846 2948 2975 1186
Size: 1160 68 432 102 27 4482
Fragments arranged by size:
4482 1160 432 102 68 27
BseYI C'CCAG_C
Cuts at: 2111 2385 3108 5474 5970 2111
Size: 274 723 2366 496 2412
Fragments arranged by size:
2412 2366 723 496 274
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5698
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2091 3721 4482 5510 1540
Size: 275 186 90 1630 761 1028 2301
Fragments arranged by size:
2301 1630 1028 761 275 186 90
BsiHKAI G_wGCw'C
Cuts at: 3074 3189 4332 4417 5488 3074
Size: 115 1143 85 1071 3857
Fragments arranged by size:
3857 1143 1071 115 85
BsmI GAATG_Cn'
Cuts at: 2488 3782 3875 2488
Size: 1294 93 4884
Fragments arranged by size:
4884 1294 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3074 3103 3189 3590 4332 4417 5488
Size: 399 1284 29 86 401 742 85 1071
Cuts at: 5488 1391
Size: 2174
Fragments arranged by size:
2174 1284 1071 742 401 399 86 85
29
BspEI T'CCGG_A
Cuts at: 5934 5934
Size: 6271
BspHI T'CATG_A
Cuts at: 4160 5890 4160
Size: 1730 4541
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3626 844
Size: 2782 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4158 1394
Size: 429 2335 3507
Fragments arranged by size:
3507 2335 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4766 4940 65
Size: 1183 3518 174 1396
Fragments arranged by size:
3518 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2147 4915 5102 1700
Size: 110 337 2768 187 2869
Fragments arranged by size:
2869 2768 337 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6271
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6271
BssSI C'ACGA_G
Cuts at: 4331 5343 4331
Size: 1012 5259
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6271
BstBI TT'CG_AA
Cuts at: 2378 5075 2378
Size: 2697 3574
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6271
BstYI r'GATC_y
Cuts at: 1972 2232 2263 3360 3601 3968 4353 4370 5822
Size: 260 31 1097 241 367 385 17 1452
Cuts at: 5822 5908 5920 6266 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1097 385 367 346 260 241
86 31 17 12
Bsu36I CC'TnA_GG
Cuts at: 2358 3552 2358
Size: 1194 5077
BtgI C'CryG_G
Cuts at: 513 1065 2004 2154 2823 513
Size: 552 939 150 669 3961
Fragments arranged by size:
3961 939 669 552 150
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5532
Fragments arranged by size:
5532 683 56
BtsI GCAGTG_nn'
Cuts at: 3281 3787 4581 4601 3281
Size: 506 794 20 4951
Fragments arranged by size:
4951 794 506 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6271
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6236
DraI TTT'AAA
Cuts at: 3575 3575
Size: 6271
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2783 5278 809
Size: 507 1467 2495 1802
Fragments arranged by size:
2495 1802 1467 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2088 3213 3718
Size: 54 1425 50 275 18 258 1125 505
Cuts at: 3718 4607 6236 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1125 889 505 275 258 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2088 3718 1537
Size: 275 276 1630 4090
Fragments arranged by size:
4090 1630 276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2355 2639 2802 4201 1353
Size: 1002 284 163 1399 3423
Fragments arranged by size:
3423 1399 1002 284 163
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2040 4858 5232 5378 387
Size: 1064 138 451 2818 374 146 1280
Fragments arranged by size:
2818 1280 1064 451 374 146 138
EcoICRI GAG'CTC
Cuts at: 3072 3187 3072
Size: 115 6156
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2087 2726 2927 4334 4931 5718 5986
Size: 393 455 639 201 1407 597 787 268
Cuts at: 5986 1239
Size: 1524
Fragments arranged by size:
1524 1407 787 639 597 455 393 268
201
EcoNI CCTnn'n_nnAGG
Cuts at: 3551 3551
Size: 6271
EcoO109I rG'GnC_Cy
Cuts at: 2776 2776
Size: 6271
EcoRI G'AATT_C
Cuts at: 3580 3958 3580
Size: 378 5893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3563 3563
Size: 6271
FspI TGC'GCA
Cuts at: 4777 4777
Size: 6271
HaeII r_GCGC'y
Cuts at: 1678 2034 2154 2971 3029 3262 5418 1678
Size: 356 120 817 58 233 2156 2531
Fragments arranged by size:
2531 2156 817 356 233 120 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2060 2092 2740 2772 3359
Size: 32 135 32 488 32 648 32 587
Cuts at: 3359 3391 4912 4944 4986 5018 1373
Size: 32 1521 32 42 32 2626
Fragments arranged by size:
2626 1521 648 587 488 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3007 3615 3861 669
Size: 988 281 1069 608 246 3079
Fragments arranged by size:
3079 1069 988 608 281 246
HindIII A'AGCT_T
Cuts at: 748 2518 2591 748
Size: 1770 73 4428
Fragments arranged by size:
4428 1770 73
HpaI GTT'AAC
Cuts at: 3861 3861
Size: 6271
KasI G'GCGC_C
Cuts at: 2150 2150
Size: 6271
KpnI G_GTAC'C
Cuts at: 2740 2832 3596 2740
Size: 92 764 5415
Fragments arranged by size:
5415 764 92
MfeI C'AATT_G
Cuts at: 3870 3870
Size: 6271
MluI A'CGCG_T
Cuts at: 6147 6147
Size: 6271
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5385 5569 1305
Size: 4080 184 2007
Fragments arranged by size:
4080 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3215 10
Size: 54 1425 343 1383 3066
Fragments arranged by size:
3066 1425 1383 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2657 2906 4229 4588 4747 518
Size: 648 1491 249 1323 359 159 2042
Fragments arranged by size:
2042 1491 1323 648 359 249 159
MspA1I CmG'CkG
Cuts at: 1606 2156 3112 3523 3687 4364 5512 5757 1606
Size: 550 956 411 164 677 1148 245 2120
Fragments arranged by size:
2120 1148 956 677 550 411 245 164
NarI GG'CG_CC
Cuts at: 2151 2151
Size: 6271
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4780
Fragments arranged by size:
4780 939 552
NdeI CA'TA_TG
Cuts at: 387 2488 387
Size: 2101 4170
NheI G'CTAG_C
Cuts at: 1052 2329 3022 1052
Size: 1277 693 4301
Fragments arranged by size:
4301 1277 693
NotI GC'GGCC_GC
Cuts at: 3718 3718
Size: 6271
NsiI A_TGCA'T
Cuts at: 2488 2488
Size: 6271
NspI r_CATG'y
Cuts at: 1132 1229 2987 3629 5174 1132
Size: 97 1758 642 1545 2229
Fragments arranged by size:
2229 1758 1545 642 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6271
PciI A'CATG_T
Cuts at: 5170 5170
Size: 6271
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6271
PfoI T'CCnGG_A
Cuts at: 2943 2943
Size: 6271
PmeI GTTT'AAAC
Cuts at: 3575 3575
Size: 6271
PmlI CAC'GTG
Cuts at: 2819 3481 2819
Size: 662 5609
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4340 4372 5853 5885 4340
Size: 32 1481 32 4726
Fragments arranged by size:
4726 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2776 2776
Size: 6271
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6271
PsiI TTA'TAA
Cuts at: 3841 3841
Size: 6271
PspXI vC'TCGA_Gb
Cuts at: 3365 3365
Size: 6271
PstI C_TGCA'G
Cuts at: 830 2701 3288 3345 3623 830
Size: 1871 587 57 278 3478
Fragments arranged by size:
3478 1871 587 278 57
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6271
PvuII CAG'CTG
Cuts at: 3112 3523 3112
Size: 411 5860
SacI G_AGCT'C
Cuts at: 3074 3189 3074
Size: 115 6156
SacII CC_GC'GG
Cuts at: 2157 2157
Size: 6271
SalI G'TCGA_C
Cuts at: 1936 3613 1936
Size: 1677 4594
SapI GCTCTTCn'nnn_
Cuts at: 2639 2802 2639
Size: 163 6108
SbfI CC_TGCA'GG
Cuts at: 3623 3623
Size: 6271
ScaI AGT'ACT
Cuts at: 3533 4519 6231 3533
Size: 986 1712 3573
Fragments arranged by size:
3573 1712 986
SfcI C'TryA_G
Cuts at: 826 1045 2026 2697 3284 3341 3619 4754 5435
Size: 219 981 671 587 57 278 1135 681
Cuts at: 5435 5626 826
Size: 191 1471
Fragments arranged by size:
1471 1135 981 681 671 587 278 219
191 57
SfoI GGC'GCC
Cuts at: 2152 2152
Size: 6271
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6271
SgrAI Cr'CCGG_yG
Cuts at: 1700 2147 1700
Size: 447 5824
SmaI CCC'GGG
Cuts at: 1872 3598 1872
Size: 1726 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2858 3365 3494 4375 5276 5538
Size: 197 926 915 507 129 881 901 262
Cuts at: 5538 820
Size: 1553
Fragments arranged by size:
1553 926 915 901 881 507 262 197
129
SnaBI TAC'GTA
Cuts at: 493 4062 493
Size: 3569 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6271
SphI G_CATG'C
Cuts at: 1132 2987 3629 1132
Size: 1855 642 3774
Fragments arranged by size:
3774 1855 642
SspI AAT'ATT
Cuts at: 5 52 4195 5
Size: 47 4143 2081
Fragments arranged by size:
4143 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6271
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 3351 3462 3710 513
Size: 552 939 291 1056 111 248 3074
Fragments arranged by size:
3074 1056 939 552 291 248 111
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4298 4315 4468 4653 5098 325
Size: 1255 2718 17 153 185 445 1498
Fragments arranged by size:
2718 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3531 4517 6229
Size: 274 80 33 51 1443 1554 986 1712
Cuts at: 6229 96
Size: 138
Fragments arranged by size:
1712 1554 1443 986 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2939 2971 6231 6263 2939
Size: 32 3260 32 2947
Fragments arranged by size:
3260 2947 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6271
XbaI T'CTAG_A
Cuts at: 3607 3607
Size: 6271
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2840 2840
Size: 6271
XhoI C'TCGA_G
Cuts at: 1943 3365 1943
Size: 1422 4849
XmaI C'CCGG_G
Cuts at: 1870 3596 1870
Size: 1726 4545
XmnI GAAnn'nnTTC
Cuts at: 2618 4400 2618
Size: 1782 4489
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2785 4079 276
Size: 53 83 186 936 1251 1294 2468
Fragments arranged by size:
2468 1294 1251 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NsiI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI PvuII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BsiWI BsmBI
BstXI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV
NruI PacI PspOMI PsrI RsrII SanDI SexAI SfiI
SrfI SwaI