Restriction map for
pFN21AB9934
(Circular) (Six-base) MAPSORT of: pFN21AB9934.seq Check: 4809 from: 1 to: 6646
pFN21AB9934 6646 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2599 3194 2599
Size: 595 6051
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4456 278
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3989 1937
Size: 2052 4594
Acc65I G'GTAC_C
Cuts at: 3967 3967
Size: 6646
AclI AA'CG_TT
Cuts at: 1563 4773 5146 1563
Size: 3210 373 3063
Fragments arranged by size:
3210 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4709 6093 6361 1632
Size: 3077 1384 268 1917
Fragments arranged by size:
3077 1917 1384 268
AfeI AGC'GCT
Cuts at: 2273 2273
Size: 6646
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6449
AflIII A'CryG_T
Cuts at: 1203 2332 5545 6522 1203
Size: 1129 3213 977 1327
Fragments arranged by size:
3213 1327 1129 977
AgeI A'CCGG_T
Cuts at: 2647 5477 2647
Size: 2830 3816
AhdI GACnn_n'nnGTC
Cuts at: 5375 5375
Size: 6646
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6646
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2952 1184
Size: 1768 4878
AlwNI CAG_nnn'CTG
Cuts at: 2128 2389 2554 2686 3382 3403 4389 5961 2128
Size: 261 165 132 696 21 986 1572 2813
Fragments arranged by size:
2813 1572 986 696 261 165 132 21
ApaI G_GGCC'C
Cuts at: 2160 2160
Size: 6646
ApaLI G'TGCA_C
Cuts at: 4703 5859 4703
Size: 1156 5490
ApoI r'AATT_y
Cuts at: 1493 3955 4181 4333 6442 1493
Size: 2462 226 152 2109 1697
Fragments arranged by size:
2462 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5200 160
Size: 872 4168 1606
Fragments arranged by size:
4168 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
AvaI C'yCGr_G
Cuts at: 1870 1943 2567 3302 3971 1870
Size: 73 624 735 669 4545
Fragments arranged by size:
4545 735 669 624 73
BamHI G'GATC_C
Cuts at: 3976 4343 3976
Size: 367 6279
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2813 3021 3206 3227 3317
Size: 325 217 627 1026 208 185 21 90
Cuts at: 3317 3802 3967 5422 618
Size: 485 165 1455 1842
Fragments arranged by size:
1842 1455 1026 627 485 325 217 208
185 165 90 21
BanII G_rGCy'C
Cuts at: 1391 2160 2292 2375 3342 3458 3522 3965 1391
Size: 769 132 83 967 116 64 443 4072
Fragments arranged by size:
4072 967 769 443 132 116 83 64
BbeI G_GCGC'C
Cuts at: 3231 3231
Size: 6646
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2055 928
Size: 971 156 5519
Fragments arranged by size:
5519 971 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4837 4871 1903
Size: 34 2900 34 3678
Fragments arranged by size:
3678 2900 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3830 4538 5748 3830
Size: 708 1210 4728
Fragments arranged by size:
4728 1210 708
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6646
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2105 2804 5257
Size: 107 122 71 631 605 433 699 2453
Cuts at: 5257 136
Size: 1525
Fragments arranged by size:
2453 1525 699 631 605 433 122 107
71
BglII A'GATC_T
Cuts at: 3132 6641 3132
Size: 3509 3137
BlpI GC'TnA_GC
Cuts at: 2523 2954 4063 2523
Size: 431 1109 5106
Fragments arranged by size:
5106 1109 431
Bme1580I G_kGCm'C
Cuts at: 1790 2160 2818 3807 4707 5863 1790
Size: 370 658 989 900 1156 2573
Fragments arranged by size:
2573 1156 989 900 658 370
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6646
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4377 5335 442
Size: 416 53 83 399 24 2960 958 1753
Fragments arranged by size:
2960 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6646
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2917 3472 2917
Size: 555 6091
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2054 2726 3295 3446 3467 3764 3899 5306
Size: 815 672 569 151 21 297 135 1407
Cuts at: 5306 1239
Size: 2579
Fragments arranged by size:
2579 1407 815 672 569 297 151 135
21
Bpu10I CC'TnA_GC
Cuts at: 3295 3295
Size: 6646
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4771 5636 5934 4771
Size: 865 298 5483
Fragments arranged by size:
5483 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2030 2447 2550 2826 3082 3321 5309
Size: 617 531 417 103 276 256 239 1988
Cuts at: 5309 882
Size: 2219
Fragments arranged by size:
2219 1988 617 531 417 276 256 239
103
BsaAI yAC'GTr
Cuts at: 493 1204 2479 3496 3910 4437 493
Size: 711 1275 1017 414 527 2702
Fragments arranged by size:
2702 1275 1017 711 527 414
BsaBI GATnn'nnATC
Cuts at: 6640 6640
Size: 6646
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3228 4453 4835 5470
Size: 53 83 186 936 1695 1225 382 635
Cuts at: 5470 275
Size: 1451
Fragments arranged by size:
1695 1451 1225 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2647 3348 3822 5079 5477 5751 5898 6309 2647
Size: 701 474 1257 398 274 147 411 2984
Fragments arranged by size:
2984 1257 701 474 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2027 2057 2492 2522 1178
Size: 30 165 30 624 30 435 30 5302
Fragments arranged by size:
5302 624 435 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2023 2081 3611 3614 3644 3896 3899 3917
Size: 837 58 1530 3 30 252 3 18
Cuts at: 3917 1186
Size: 3915
Fragments arranged by size:
3915 1530 837 252 58 30 18 3
3
BseYI C'CCAG_C
Cuts at: 2121 2639 2668 3489 3523 3738 5849 6345 2121
Size: 518 29 821 34 215 2111 496 2422
Fragments arranged by size:
2422 2111 821 518 496 215 34 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2011 2431 3657 3932 1163
Size: 573 275 420 1226 275 3877
Fragments arranged by size:
3877 1226 573 420 275 275
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2112 2328 4096 4857 5885 1540
Size: 275 186 111 216 1768 761 1028 2301
Fragments arranged by size:
2301 1768 1028 761 275 216 186 111
BsiHKAI G_wGCw'C
Cuts at: 2375 3342 3458 3909 4707 4792 5863 2375
Size: 967 116 451 798 85 1071 3158
Fragments arranged by size:
3158 1071 967 798 451 116 85
BsmI GAATG_Cn'
Cuts at: 4157 4250 4157
Size: 93 6553
BsmBI CGTCTCn'nnnn_
Cuts at: 3719 3719
Size: 6646
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2160 2292 2375 2818 3342 3458 3522
Size: 399 370 132 83 443 524 116 64
Cuts at: 3522 3807 3909 3965 4707 4792 5863 1391
Size: 285 102 56 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 524 443 399 370 285
132 116 102 85 83 64 56
BspEI T'CCGG_A
Cuts at: 3348 3822 6309 3348
Size: 474 2487 3685
Fragments arranged by size:
3685 2487 474
BspHI T'CATG_A
Cuts at: 4535 6265 4535
Size: 1730 4916
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2599 3194 4001 844
Size: 1755 595 807 3489
Fragments arranged by size:
3489 1755 807 595
BsrBI CCG'CTC
Cuts at: 1394 1823 2013 2077 2286 2527 3634 4533 1394
Size: 429 190 64 209 241 1107 899 3507
Fragments arranged by size:
3507 1107 899 429 241 209 190 64
BsrDI GCAATG_nn'
Cuts at: 65 1248 3249 5141 5315 65
Size: 1183 2001 1892 174 1396
Fragments arranged by size:
2001 1892 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2647 2798 2835 5290 5477 1700
Size: 110 837 151 37 2455 187 2869
Fragments arranged by size:
2869 2455 837 187 151 110 37
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6646
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6646
BssSI C'ACGA_G
Cuts at: 2093 4706 5718 2093
Size: 2613 1012 3021
Fragments arranged by size:
3021 2613 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6646
BstBI TT'CG_AA
Cuts at: 5450 5450
Size: 6646
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6646
BstXI CCAn_nnnn'nTGG
Cuts at: 3700 3700
Size: 6646
BstYI r'GATC_y
Cuts at: 1972 2490 3132 3976 4343 4728 4745 6197 6283
Size: 518 642 844 367 385 17 1452 86
Cuts at: 6283 6295 6641 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 844 642 518 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2175 2175
Size: 6646
BtgI C'CryG_G
Cuts at: 513 1065 2298 2452 3331 3474 3807 513
Size: 552 1233 154 879 143 333 3352
Fragments arranged by size:
3352 1233 879 552 333 154 143
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5907
Fragments arranged by size:
5907 683 56
BtsI GCAGTG_nn'
Cuts at: 3592 4162 4956 4976 3592
Size: 570 794 20 5262
Fragments arranged by size:
5262 794 570 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6646
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6611
DraI TTT'AAA
Cuts at: 3950 3950
Size: 6646
DraIII CAC_nnn'GTG
Cuts at: 2479 2479
Size: 6646
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3779 5653 809
Size: 507 2463 1874 1802
Fragments arranged by size:
2463 1874 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2109 2161 2325
Size: 54 1425 50 275 18 279 52 164
Cuts at: 2325 2613 2656 2761 2805 3463 3681 4093 4982
Size: 288 43 105 44 658 218 412 889
Cuts at: 4982 6611 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 658 412 288 279 275
218 164 105 54 52 50 44 43
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2109 2325 4093 1537
Size: 275 297 216 1768 4090
Fragments arranged by size:
4090 1768 297 275 216
EarI CTCTTCn'nnn_
Cuts at: 1353 2225 3446 4576 1353
Size: 872 1221 1130 3423
Fragments arranged by size:
3423 1221 1130 872
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2107 2207 5233 5607 5753 387
Size: 1064 138 518 100 3026 374 146 1280
Fragments arranged by size:
3026 1280 1064 518 374 146 138 100
EcoICRI GAG'CTC
Cuts at: 2373 3340 3456 2373
Size: 967 116 5563
Fragments arranged by size:
5563 967 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2054 2726 3295 3446 3467 3764 3899
Size: 393 422 672 569 151 21 297 135
Cuts at: 3899 4709 5306 6093 6361 1239
Size: 810 597 787 268 1524
Fragments arranged by size:
1524 810 787 672 597 569 422 393
297 268 151 135 21
EcoNI CCTnn'n_nnAGG
Cuts at: 2807 2930 3201 2807
Size: 123 271 6252
Fragments arranged by size:
6252 271 123
EcoO109I rG'GnC_Cy
Cuts at: 2551 2749 2896 2944 3004 3299 3306 2551
Size: 198 147 48 60 295 7 5891
Fragments arranged by size:
5891 295 198 147 60 48 7
EcoRI G'AATT_C
Cuts at: 3955 4333 3955
Size: 378 6268
EcoRV GAT'ATC
Cuts at: 3925 3925
Size: 6646
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 6646
FspI TGC'GCA
Cuts at: 5152 5152
Size: 6646
HaeII r_GCGC'y
Cuts at: 1678 2083 2275 2979 3231 5793 1678
Size: 405 192 704 252 2562 2531
Fragments arranged by size:
2562 2531 704 405 252 192
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3255 3287 3343 3375 5287
Size: 32 135 32 1683 32 56 32 1912
Cuts at: 5287 5319 5361 5393 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1912 1683 135 56 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2500 3990 4236 669
Size: 988 281 562 1490 246 3079
Fragments arranged by size:
3079 1490 988 562 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6646
HpaI GTT'AAC
Cuts at: 4236 4236
Size: 6646
KasI G'GCGC_C
Cuts at: 3227 3227
Size: 6646
KpnI G_GTAC'C
Cuts at: 3971 3971
Size: 6646
MfeI C'AATT_G
Cuts at: 4245 4245
Size: 6646
MluI A'CGCG_T
Cuts at: 6522 6522
Size: 6646
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5760 5944 1305
Size: 4455 184 2007
Fragments arranged by size:
4455 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2658 3465 3683 10
Size: 54 1425 343 826 807 218 2973
Fragments arranged by size:
2973 1425 826 807 343 218 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2061 4604 4963 5122 518
Size: 648 895 2543 359 159 2042
Fragments arranged by size:
2543 2042 895 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2128 2300 2668 3315 3324 3622 3742 3769
Size: 522 172 368 647 9 298 120 27
Cuts at: 3769 4062 4739 5887 6132 1606
Size: 293 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 647 522 368 298 293
245 172 120 27 9
NaeI GCC'GGC
Cuts at: 2800 2800
Size: 6646
NarI GG'CG_CC
Cuts at: 3228 3228
Size: 6646
NcoI C'CATG_G
Cuts at: 513 1065 2452 513
Size: 552 1387 4707
Fragments arranged by size:
4707 1387 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6646
NgoMIV G'CCGG_C
Cuts at: 2798 2798
Size: 6646
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6646
NotI GC'GGCC_GC
Cuts at: 2109 4093 2109
Size: 1984 4662
NspI r_CATG'y
Cuts at: 1132 1229 4004 5549 1132
Size: 97 2775 1545 2229
Fragments arranged by size:
2775 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2771 1348
Size: 1423 5223
PciI A'CATG_T
Cuts at: 5545 5545
Size: 6646
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 2611 1108
Size: 1281 222 5143
Fragments arranged by size:
5143 1281 222
PmeI GTTT'AAAC
Cuts at: 3950 3950
Size: 6646
PmlI CAC'GTG
Cuts at: 2479 3910 2479
Size: 1431 5215
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4715 4747 6228 6260 4715
Size: 32 1481 32 5101
Fragments arranged by size:
5101 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6646
PsiI TTA'TAA
Cuts at: 4216 4216
Size: 6646
PspOMI G'GGCC_C
Cuts at: 2156 2156
Size: 6646
PstI C_TGCA'G
Cuts at: 830 2259 2682 2931 3408 3998 830
Size: 1429 423 249 477 590 3478
Fragments arranged by size:
3478 1429 590 477 423 249
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6646
PvuII CAG'CTG
Cuts at: 2128 3622 3742 3769 2128
Size: 1494 120 27 5005
Fragments arranged by size:
5005 1494 120 27
SacI G_AGCT'C
Cuts at: 2375 3342 3458 2375
Size: 967 116 5563
Fragments arranged by size:
5563 967 116
SacII CC_GC'GG
Cuts at: 2301 2301
Size: 6646
SalI G'TCGA_C
Cuts at: 1936 3988 1936
Size: 2052 4594
SapI GCTCTTCn'nnn_
Cuts at: 2225 3446 2225
Size: 1221 5425
SbfI CC_TGCA'GG
Cuts at: 2682 3998 2682
Size: 1316 5330
ScaI AGT'ACT
Cuts at: 4894 6606 4894
Size: 1712 4934
SexAI A'CCwGG_T
Cuts at: 2460 2460
Size: 6646
SfcI C'TryA_G
Cuts at: 826 1045 2255 2678 2927 3404 3830 3994 5129
Size: 219 1210 423 249 477 426 164 1135
Cuts at: 5129 5810 6001 826
Size: 681 191 1471
Fragments arranged by size:
1471 1210 1135 681 477 426 423 249
219 191 164
SfoI GGC'GCC
Cuts at: 3229 3229
Size: 6646
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6646
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6646
SmaI CCC'GGG
Cuts at: 1872 3304 3973 1872
Size: 1432 669 4545
Fragments arranged by size:
4545 1432 669
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4750 5651 5913 820
Size: 197 926 2807 901 262 1553
Fragments arranged by size:
2807 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4437 493
Size: 3944 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6646
SphI G_CATG'C
Cuts at: 1132 4004 1132
Size: 2872 3774
SspI AAT'ATT
Cuts at: 5 52 4570 5
Size: 47 4518 2081
Fragments arranged by size:
4518 2081 47
StuI AGG'CCT
Cuts at: 1517 2021 3749 1517
Size: 504 1728 4414
Fragments arranged by size:
4414 1728 504
StyI C'CwwG_G
Cuts at: 513 1065 2427 2435 2452 3713 4085 513
Size: 552 1362 8 17 1261 372 3074
Fragments arranged by size:
3074 1362 1261 552 372 17 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2465 3051 4673 4690 4843 5028 5473
Size: 1255 885 586 1622 17 153 185 445
Cuts at: 5473 325
Size: 1498
Fragments arranged by size:
1622 1498 1255 885 586 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4892 6604 96
Size: 274 80 33 51 1443 2915 1712 138
Fragments arranged by size:
2915 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2027 2059 2448 2480 6606 6638 2027
Size: 32 389 32 4126 32 2035
Fragments arranged by size:
4126 2035 389 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3054 1316
Size: 1738 4908
XbaI T'CTAG_A
Cuts at: 3982 3982
Size: 6646
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3100 3364 3383 3421 3511 3100
Size: 264 19 38 90 6235
Fragments arranged by size:
6235 264 90 38 19
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6646
XmaI C'CCGG_G
Cuts at: 1870 3302 3971 1870
Size: 1432 669 4545
Fragments arranged by size:
4545 1432 669
XmnI GAAnn'nnTTC
Cuts at: 4775 4775
Size: 6646
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4454 276
Size: 53 83 186 936 2920 2468
Fragments arranged by size:
2920 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI
PvuII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstZ17I
FseI FspAI NruI NsiI PacI PfoI PpuMI PspXI
PsrI RsrII SanDI SfiI SrfI SwaI