Restriction map for
pFN21AB9933
(Circular) (Six-base) MAPSORT of: pFN21AB9933.seq Check: 3811 from: 1 to: 6172
pFN21AB9933 6172 bp
With 182 enzymes: SgfI *
April 15, 2010 09:28 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3982 278
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2850 3515 1937
Size: 913 665 4594
Fragments arranged by size:
4594 913 665
Acc65I G'GTAC_C
Cuts at: 3493 3493
Size: 6172
AclI AA'CG_TT
Cuts at: 1563 4299 4672 1563
Size: 2736 373 3063
Fragments arranged by size:
3063 2736 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2704 3236 4235 5619 5887 1632
Size: 1072 532 999 1384 268 1917
Fragments arranged by size:
1917 1384 1072 999 532 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5975
AflIII A'CryG_T
Cuts at: 1203 2928 3234 5071 6048 1203
Size: 1725 306 1837 977 1327
Fragments arranged by size:
1837 1725 1327 977 306
AgeI A'CCGG_T
Cuts at: 5003 5003
Size: 6172
AhdI GACnn_n'nnGTC
Cuts at: 4901 4901
Size: 6172
AleI CACnn'nnGTG
Cuts at: 1166 2633 2843 1166
Size: 1467 210 4495
Fragments arranged by size:
4495 1467 210
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6172
AlwNI CAG_nnn'CTG
Cuts at: 2794 3382 3915 5487 2794
Size: 588 533 1572 3479
Fragments arranged by size:
3479 1572 588 533
ApaI G_GGCC'C
Cuts at: 2668 2815 3114 3289 3391 2668
Size: 147 299 175 102 5449
Fragments arranged by size:
5449 299 175 147 102
ApaLI G'TGCA_C
Cuts at: 4229 5385 4229
Size: 1156 5016
ApoI r'AATT_y
Cuts at: 1493 2036 3481 3707 3859 5968 1493
Size: 543 1445 226 152 2109 1697
Fragments arranged by size:
2109 1697 1445 543 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4726 160
Size: 872 3694 1606
Fragments arranged by size:
3694 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
AvaI C'yCGr_G
Cuts at: 1870 1943 2768 2800 3113 3265 3497 1870
Size: 73 825 32 313 152 232 4545
Fragments arranged by size:
4545 825 313 232 152 73 32
AvrII C'CTAG_G
Cuts at: 3280 3280
Size: 6172
BamHI G'GATC_C
Cuts at: 3502 3869 3502
Size: 367 5805
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2177 2424 2627 3493 4948
Size: 325 217 627 390 247 203 866 1455
Cuts at: 4948 618
Size: 1842
Fragments arranged by size:
1842 1455 866 627 390 325 247 217
203
BanII G_rGCy'C
Cuts at: 1391 2668 2815 3045 3114 3272 3289 3391 3491
Size: 1277 147 230 69 158 17 102 100
Cuts at: 3491 1391
Size: 4072
Fragments arranged by size:
4072 1277 230 158 147 102 100 69
17
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5201
BbvCI CC'TCA_GC
Cuts at: 3132 3132
Size: 6172
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4363 4397 1903
Size: 34 2426 34 3678
Fragments arranged by size:
3678 2426 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4064 5274 4064
Size: 1210 4962
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6172
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3128 3350 3395
Size: 107 122 71 631 605 1456 222 45
Cuts at: 3395 4783 136
Size: 1388 1525
Fragments arranged by size:
1525 1456 1388 631 605 222 122 107
71 45
BglII A'GATC_T
Cuts at: 6167 6167
Size: 6172
BlpI GC'TnA_GC
Cuts at: 2581 3589 2581
Size: 1008 5164
Bme1580I G_kGCm'C
Cuts at: 1790 2247 2397 2495 2630 2668 2815 3114 3198
Size: 457 150 98 135 38 147 299 84
Cuts at: 3198 3289 3391 4233 5389 1790
Size: 91 102 842 1156 2573
Fragments arranged by size:
2573 1156 842 457 299 150 147 135
102 98 91 84 38
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6172
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3903 4861 442
Size: 416 53 83 399 24 2486 958 1753
Fragments arranged by size:
2486 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6172
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2857 3005 3146 3383 3455 4832 1239
Size: 1169 449 148 141 237 72 1377 2579
Fragments arranged by size:
2579 1377 1169 449 237 148 141 72
Bpu10I CC'TnA_GC
Cuts at: 2198 3132 2198
Size: 934 5238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4297 5162 5460 4297
Size: 865 298 5009
Fragments arranged by size:
5009 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4835 882
Size: 617 3336 2219
Fragments arranged by size:
3336 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3193 3963 493
Size: 711 1989 770 2702
Fragments arranged by size:
2702 1989 770 711
BsaBI GATnn'nnATC
Cuts at: 3170 6166 3170
Size: 2996 3176
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3979 4361 4996 275
Size: 53 83 186 936 2446 382 635 1451
Fragments arranged by size:
2446 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2880 4605 5003 5277 5424 5835 2880
Size: 1725 398 274 147 411 3217
Fragments arranged by size:
3217 1725 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2492 2522 1178
Size: 30 165 30 1089 30 4828
Fragments arranged by size:
4828 1089 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2516 2915 3149 3262 3380 3452 1186
Size: 1330 399 234 113 118 72 3906
Fragments arranged by size:
3906 1330 399 234 118 113 72
BseYI C'CCAG_C
Cuts at: 3197 3323 3353 3445 5375 5871 3197
Size: 126 30 92 1930 496 3498
Fragments arranged by size:
3498 1930 496 126 92 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2302 2475 2510 1163
Size: 573 566 173 35 4825
Fragments arranged by size:
4825 573 566 173 35
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3421 3622 4383 5411 1540
Size: 275 186 1420 201 761 1028 2301
Fragments arranged by size:
2301 1420 1028 761 275 201 186
BsiHKAI G_wGCw'C
Cuts at: 2193 4233 4318 5389 2193
Size: 2040 85 1071 2976
Fragments arranged by size:
2976 2040 1071 85
BsmI GAATG_Cn'
Cuts at: 3683 3776 3683
Size: 93 6079
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2193 2247 2397 2495 2630 2668 2815
Size: 399 403 54 150 98 135 38 147
Cuts at: 2815 3045 3114 3198 3272 3289 3391 3491 4233
Size: 230 69 84 74 17 102 100 742
Cuts at: 4233 4318 5389 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 403 399 230 150 147
135 102 100 98 85 84 74 69
54 38 17
BspEI T'CCGG_A
Cuts at: 5835 5835
Size: 6172
BspHI T'CATG_A
Cuts at: 2656 3051 4061 5791 2656
Size: 395 1010 1730 3037
Fragments arranged by size:
3037 1730 1010 395
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3527 844
Size: 2683 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2644 2737 3147 4059 1394
Size: 429 821 93 410 912 3507
Fragments arranged by size:
3507 912 821 429 410 93
BsrDI GCAATG_nn'
Cuts at: 65 1248 4667 4841 65
Size: 1183 3419 174 1396
Fragments arranged by size:
3419 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2059 2374 3225 3249 4816 5003 1700
Size: 110 249 315 851 24 1567 187 2869
Fragments arranged by size:
2869 1567 851 315 249 187 110 24
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6172
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6172
BssSI C'ACGA_G
Cuts at: 4232 5244 4232
Size: 1012 5160
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2124 3382 794
Size: 1330 1258 3584
Fragments arranged by size:
3584 1330 1258
BstBI TT'CG_AA
Cuts at: 4976 4976
Size: 6172
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6172
BstXI CCAn_nnnn'nTGG
Cuts at: 2146 2407 2689 2146
Size: 261 282 5629
Fragments arranged by size:
5629 282 261
BstYI r'GATC_y
Cuts at: 1972 2337 3502 3869 4254 4271 5723 5809 5821
Size: 365 1165 367 385 17 1452 86 12
Cuts at: 5821 6167 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1165 385 367 365 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 3392 3392
Size: 6172
BtgI C'CryG_G
Cuts at: 513 1065 2430 2778 3258 513
Size: 552 1365 348 480 3427
Fragments arranged by size:
3427 1365 552 480 348
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2401 487
Size: 683 56 1175 4258
Fragments arranged by size:
4258 1175 683 56
BtsI GCAGTG_nn'
Cuts at: 3176 3303 3688 4482 4502 3176
Size: 127 385 794 20 4846
Fragments arranged by size:
4846 794 385 127 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6172
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6137
DraI TTT'AAA
Cuts at: 3476 3476
Size: 6172
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2321 5179 809
Size: 507 1005 2858 1802
Fragments arranged by size:
2858 1802 1005 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3336 3619 4508
Size: 54 1425 50 275 18 1506 283 889
Cuts at: 4508 6137 8
Size: 1629 43
Fragments arranged by size:
1629 1506 1425 889 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3619 1537
Size: 275 1807 4090
Fragments arranged by size:
4090 1807 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3083 4102 1353
Size: 1730 1019 3423
Fragments arranged by size:
3423 1730 1019
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4759 5133 5279 387
Size: 1064 138 3170 374 146 1280
Fragments arranged by size:
3170 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2408 2704 2857 3005 3146 3236 3383
Size: 393 776 296 153 148 141 90 147
Cuts at: 3383 3455 4235 4832 5619 5887 1239
Size: 72 780 597 787 268 1524
Fragments arranged by size:
1524 787 780 776 597 393 296 268
153 148 147 141 90 72
EcoNI CCTnn'n_nnAGG
Cuts at: 3080 3080
Size: 6172
EcoO109I rG'GnC_Cy
Cuts at: 2252 2664 2755 2811 2812 2980 3211 3285 3286
Size: 412 91 56 1 168 231 74 1
Cuts at: 3286 3388 2252
Size: 102 5036
Fragments arranged by size:
5036 412 231 168 102 91 74 56
1 1
EcoRI G'AATT_C
Cuts at: 3481 3859 3481
Size: 378 5794
FspI TGC'GCA
Cuts at: 2606 4678 2606
Size: 2072 4100
FspAI rTGC'GCAy
Cuts at: 2606 2606
Size: 6172
HaeII r_GCGC'y
Cuts at: 1678 2750 3401 5319 1678
Size: 1072 651 1918 2531
Fragments arranged by size:
2531 1918 1072 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2397 2429 4813 4845 4887
Size: 32 135 32 825 32 2384 32 42
Cuts at: 4887 4919 1373
Size: 32 2626
Fragments arranged by size:
2626 2384 825 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2134 3516 3762 669
Size: 988 281 196 1382 246 3079
Fragments arranged by size:
3079 1382 988 281 246 196
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6172
HpaI GTT'AAC
Cuts at: 3762 3762
Size: 6172
KpnI G_GTAC'C
Cuts at: 3497 3497
Size: 6172
MfeI C'AATT_G
Cuts at: 3771 3771
Size: 6172
MluI A'CGCG_T
Cuts at: 6048 6048
Size: 6172
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3051 5286 5470 1305
Size: 1746 2235 184 2007
Fragments arranged by size:
2235 2007 1746 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3338 10
Size: 54 1425 343 1506 2844
Fragments arranged by size:
2844 1506 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2093 2324 2633 2843 3050 3056 4130
Size: 648 927 231 309 210 207 6 1074
Cuts at: 4130 4489 4648 518
Size: 359 159 2042
Fragments arranged by size:
2042 1074 927 648 359 309 231 210
207 159 6
MspA1I CmG'CkG
Cuts at: 1606 2304 2365 2498 2553 2743 2953 3208 3327
Size: 698 61 133 55 190 210 255 119
Cuts at: 3327 3445 3588 4265 5413 5658 1606
Size: 118 143 677 1148 245 2120
Fragments arranged by size:
2120 1148 698 677 255 245 210 190
143 133 119 118 61 55
NaeI GCC'GGC
Cuts at: 3227 3251 3227
Size: 24 6148
NcoI C'CATG_G
Cuts at: 513 1065 2778 3258 513
Size: 552 1713 480 3427
Fragments arranged by size:
3427 1713 552 480
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6172
NgoMIV G'CCGG_C
Cuts at: 3225 3249 3225
Size: 24 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6172
NotI GC'GGCC_GC
Cuts at: 3619 3619
Size: 6172
NspI r_CATG'y
Cuts at: 1132 1229 2048 2589 2971 3530 5075 1132
Size: 97 819 541 382 559 1545 2229
Fragments arranged by size:
2229 1545 819 559 541 382 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6172
PciI A'CATG_T
Cuts at: 5071 5071
Size: 6172
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6172
PfoI T'CCnGG_A
Cuts at: 2259 3081 2259
Size: 822 5350
PmeI GTTT'AAAC
Cuts at: 3476 3476
Size: 6172
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4241 4273 5754 5786 4241
Size: 32 1481 32 4627
Fragments arranged by size:
4627 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2252 2755 3211 2252
Size: 503 456 5213
Fragments arranged by size:
5213 503 456
PshAI GACnn'nnGTC
Cuts at: 1613 2274 2619 1613
Size: 661 345 5166
Fragments arranged by size:
5166 661 345
PsiI TTA'TAA
Cuts at: 3742 3742
Size: 6172
PspOMI G'GGCC_C
Cuts at: 2664 2811 3110 3285 3387 2664
Size: 147 299 175 102 5449
Fragments arranged by size:
5449 299 175 147 102
PspXI vC'TCGA_Gb
Cuts at: 2768 2800 2768
Size: 32 6140
PstI C_TGCA'G
Cuts at: 830 2790 3524 830
Size: 1960 734 3478
Fragments arranged by size:
3478 1960 734
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6172
PvuII CAG'CTG
Cuts at: 2553 2743 3445 2553
Size: 190 702 5280
Fragments arranged by size:
5280 702 190
SalI G'TCGA_C
Cuts at: 1936 3514 1936
Size: 1578 4594
SanDI GG'GwC_CC
Cuts at: 2755 2755
Size: 6172
SbfI CC_TGCA'GG
Cuts at: 2790 3524 2790
Size: 734 5438
ScaI AGT'ACT
Cuts at: 4420 6132 4420
Size: 1712 4460
SfcI C'TryA_G
Cuts at: 826 1045 2786 3520 4655 5336 5527 826
Size: 219 1741 734 1135 681 191 1471
Fragments arranged by size:
1741 1471 1135 734 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6172
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6172
SmaI CCC'GGG
Cuts at: 1872 3267 3499 1872
Size: 1395 232 4545
Fragments arranged by size:
4545 1395 232
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2768 2800 4276 5177 5439 820
Size: 197 926 825 32 1476 901 262 1553
Fragments arranged by size:
1553 1476 926 901 825 262 197 32
SnaBI TAC'GTA
Cuts at: 493 3963 493
Size: 3470 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6172
SphI G_CATG'C
Cuts at: 1132 2589 2971 3530 1132
Size: 1457 382 559 3774
Fragments arranged by size:
3774 1457 559 382
SspI AAT'ATT
Cuts at: 5 52 4096 5
Size: 47 4044 2081
Fragments arranged by size:
4044 2081 47
StuI AGG'CCT
Cuts at: 1517 2225 3188 1517
Size: 708 963 4501
Fragments arranged by size:
4501 963 708
StyI C'CwwG_G
Cuts at: 513 1065 2778 3258 3280 3611 513
Size: 552 1713 480 22 331 3074
Fragments arranged by size:
3074 1713 552 480 331 22
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3130 4199 4216 4369 4554 4999 325
Size: 1255 1550 1069 17 153 185 445 1498
Fragments arranged by size:
1550 1498 1255 1069 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4418 6130 96
Size: 274 80 33 51 1443 2441 1712 138
Fragments arranged by size:
2441 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6132 6164 6132
Size: 32 6140
Tth111I GACn'n_nGTC
Cuts at: 1316 2291 1316
Size: 975 5197
XbaI T'CTAG_A
Cuts at: 3508 3508
Size: 6172
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2980 3331 2980
Size: 351 5821
XhoI C'TCGA_G
Cuts at: 1943 2768 2800 1943
Size: 825 32 5315
Fragments arranged by size:
5315 825 32
XmaI C'CCGG_G
Cuts at: 1870 3265 3497 1870
Size: 1395 232 4545
Fragments arranged by size:
4545 1395 232
XmnI GAAnn'nnTTC
Cuts at: 2930 4301 2930
Size: 1371 4801
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3980 276
Size: 53 83 186 936 2446 2468
Fragments arranged by size:
2468 2446 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI
PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV FalI FseI
KasI NarI NruI NsiI PacI PmlI PsrI RsrII
SacI SacII SapI SexAI SfiI SfoI SrfI SwaI