Restriction map for
pFN21AB9930
(Circular) (Six-base) MAPSORT of: pFN21AB9930.seq Check: 5764 from: 1 to: 6406
pFN21AB9930 6406 bp
With 182 enzymes: SgfI *
August 16, 2010 14:55 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4216 278
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3749 1937
Size: 1812 4594
Acc65I G'GTAC_C
Cuts at: 2769 3727 2769
Size: 958 5448
AclI AA'CG_TT
Cuts at: 1563 4533 4906 1563
Size: 2970 373 3063
Fragments arranged by size:
3063 2970 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2537 2549 3485 4469 5853 6121 1632
Size: 905 12 936 984 1384 268 1917
Fragments arranged by size:
1917 1384 984 936 905 268 12
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6209
AflIII A'CryG_T
Cuts at: 1203 3051 5305 6282 1203
Size: 1848 2254 977 1327
Fragments arranged by size:
2254 1848 1327 977
AgeI A'CCGG_T
Cuts at: 5237 5237
Size: 6406
AhdI GACnn_n'nnGTC
Cuts at: 5135 5135
Size: 6406
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6406
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2712 1184
Size: 1528 4878
AlwNI CAG_nnn'CTG
Cuts at: 2515 4149 5721 2515
Size: 1634 1572 3200
Fragments arranged by size:
3200 1634 1572
ApaLI G'TGCA_C
Cuts at: 4463 5619 4463
Size: 1156 5250
ApoI r'AATT_y
Cuts at: 1493 2080 3715 3941 4093 6202 1493
Size: 587 1635 226 152 2109 1697
Fragments arranged by size:
2109 1697 1635 587 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4960 160
Size: 872 3928 1606
Fragments arranged by size:
3928 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
AvaI C'yCGr_G
Cuts at: 1870 1943 3731 1870
Size: 73 1788 4545
Fragments arranged by size:
4545 1788 73
BamHI G'GATC_C
Cuts at: 3736 4103 3736
Size: 367 6039
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2769 3727 5182 618
Size: 325 217 627 982 958 1455 1842
Fragments arranged by size:
1842 1455 982 958 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3725 1391
Size: 2334 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2039 2707 928
Size: 971 140 668 4627
Fragments arranged by size:
4627 971 668 140
BbvCI CC'TCA_GC
Cuts at: 3368 3368
Size: 6406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4597 4631 1903
Size: 34 2660 34 3678
Fragments arranged by size:
3678 2660 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4298 5508 4298
Size: 1210 5196
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6406
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2832 3149 5017
Size: 107 122 71 631 605 1160 317 1868
Cuts at: 5017 136
Size: 1525
Fragments arranged by size:
1868 1525 1160 631 605 317 122 107
71
BglII A'GATC_T
Cuts at: 6401 6401
Size: 6406
BlpI GC'TnA_GC
Cuts at: 2900 3608 3823 2900
Size: 708 215 5483
Fragments arranged by size:
5483 708 215
Bme1580I G_kGCm'C
Cuts at: 1790 3069 4467 5623 1790
Size: 1279 1398 1156 2573
Fragments arranged by size:
2573 1398 1279 1156
BmgBI CAC'GTC
Cuts at: 1318 3567 3628 1318
Size: 2249 61 4096
Fragments arranged by size:
4096 2249 61
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2656 4137 5095
Size: 416 53 83 399 24 1239 1481 958
Cuts at: 5095 442
Size: 1753
Fragments arranged by size:
1753 1481 1239 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2890 3322 2890
Size: 432 5974
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3081 3410 5066 1239
Size: 1842 329 1656 2579
Fragments arranged by size:
2579 1842 1656 329
Bpu10I CC'TnA_GC
Cuts at: 2967 3368 3492 2967
Size: 401 124 5881
Fragments arranged by size:
5881 401 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3211 4531 5396 5694 3211
Size: 1320 865 298 3923
Fragments arranged by size:
3923 1320 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5069 882
Size: 617 3570 2219
Fragments arranged by size:
3570 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4197 493
Size: 711 2993 2702
Fragments arranged by size:
2993 2702 711
BsaBI GATnn'nnATC
Cuts at: 6400 6400
Size: 6406
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4213 4595 5230 275
Size: 53 83 186 936 2680 382 635 1451
Fragments arranged by size:
2680 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2766 4839 5237 5511 5658 6069 2766
Size: 2073 398 274 147 411 3103
Fragments arranged by size:
3103 2073 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3163 3193 1178
Size: 30 165 30 1760 30 4391
Fragments arranged by size:
4391 1760 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2251 2324 2327 2646 3000 1186
Size: 1065 73 3 319 354 4592
Fragments arranged by size:
4592 1065 354 319 73 3
BseYI C'CCAG_C
Cuts at: 3309 5609 6105 3309
Size: 2300 496 3610
Fragments arranged by size:
3610 2300 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3650 3681 1163
Size: 573 1914 31 3888
Fragments arranged by size:
3888 1914 573 31
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3538 3856 4617 5645 1540
Size: 275 186 1537 318 761 1028 2301
Fragments arranged by size:
2301 1537 1028 761 318 275 186
BsiHKAI G_wGCw'C
Cuts at: 2077 2580 2735 4467 4552 5623 2077
Size: 503 155 1732 85 1071 2860
Fragments arranged by size:
2860 1732 1071 503 155 85
BsmI GAATG_Cn'
Cuts at: 2869 3917 4010 2869
Size: 1048 93 5265
Fragments arranged by size:
5265 1048 93
BsmBI CGTCTCn'nnnn_
Cuts at: 3287 3618 3287
Size: 331 6075
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2077 2580 2735 3069 3725 4467 4552
Size: 399 287 503 155 334 656 742 85
Cuts at: 4552 5623 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 656 503 399 334 287
155 85
BspEI T'CCGG_A
Cuts at: 6069 6069
Size: 6406
BspHI T'CATG_A
Cuts at: 4295 6025 4295
Size: 1730 4676
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3761 844
Size: 2917 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3612 4293 1394
Size: 429 1789 681 3507
Fragments arranged by size:
3507 1789 681 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4901 5075 65
Size: 1183 3653 174 1396
Fragments arranged by size:
3653 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2831 3537 3572 5050 5237 1700
Size: 110 1021 706 35 1478 187 2869
Fragments arranged by size:
2869 1478 1021 706 187 110 35
BsrGI T'GTAC_A
Cuts at: 96 2134 96
Size: 2038 4368
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6406
BssSI C'ACGA_G
Cuts at: 2938 3296 4466 5478 2938
Size: 358 1170 1012 3866
Fragments arranged by size:
3866 1170 1012 358
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6406
BstBI TT'CG_AA
Cuts at: 5210 5210
Size: 6406
BstEII G'GTnAC_C
Cuts at: 1184 3504 1184
Size: 2320 4086
BstXI CCAn_nnnn'nTGG
Cuts at: 3349 3349
Size: 6406
BstYI r'GATC_y
Cuts at: 1972 3261 3736 4103 4488 4505 5957 6043 6055
Size: 1289 475 367 385 17 1452 86 12
Cuts at: 6055 6401 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1289 475 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2037 2724 3039 3526 513
Size: 552 972 687 315 487 3393
Fragments arranged by size:
3393 972 687 552 487 315
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3501 487
Size: 683 56 2275 3392
Fragments arranged by size:
3392 2275 683 56
BtsI GCAGTG_nn'
Cuts at: 2789 3922 4716 4736 2789
Size: 1133 794 20 4459
Fragments arranged by size:
4459 1133 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6406
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6371
DraI TTT'AAA
Cuts at: 3710 3710
Size: 6406
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5413 809
Size: 507 4097 1802
Fragments arranged by size:
4097 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2824 3535 3539
Size: 54 1425 50 275 18 994 711 4
Cuts at: 3539 3853 4742 6371 8
Size: 314 889 1629 43
Fragments arranged by size:
1629 1425 994 889 711 314 275 54
50 43 18 4
EagI C'GGCC_G
Cuts at: 1537 1812 3535 3853 1537
Size: 275 1723 318 4090
Fragments arranged by size:
4090 1723 318 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2042 3441 3468 3669 4336 1353
Size: 689 1399 27 201 667 3423
Fragments arranged by size:
3423 1399 689 667 201 27
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3275 3368 4993 5367 5513 387
Size: 1064 138 1686 93 1625 374 146 1280
Fragments arranged by size:
1686 1625 1280 1064 374 146 138 93
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2537 2549 3081 3410 3485 4469 5066
Size: 393 905 12 532 329 75 984 597
Cuts at: 5066 5853 6121 1239
Size: 787 268 1524
Fragments arranged by size:
1524 984 905 787 597 532 393 329
268 75 12
EcoRI G'AATT_C
Cuts at: 3715 4093 3715
Size: 378 6028
EcoRV GAT'ATC
Cuts at: 2167 2167
Size: 6406
FseI GG_CCGG'CC
Cuts at: 3541 3541
Size: 6406
FspI TGC'GCA
Cuts at: 4912 4912
Size: 6406
HaeII r_GCGC'y
Cuts at: 1678 2290 5553 1678
Size: 612 3263 2531
Fragments arranged by size:
3263 2531 612
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3163 3195 3265 3297 5047
Size: 32 135 32 1591 32 70 32 1750
Cuts at: 5047 5079 5121 5153 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1750 1591 135 70 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2385 3750 3996 669
Size: 988 281 447 1365 246 3079
Fragments arranged by size:
3079 1365 988 447 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6406
HpaI GTT'AAC
Cuts at: 3996 3996
Size: 6406
KpnI G_GTAC'C
Cuts at: 2773 3731 2773
Size: 958 5448
MfeI C'AATT_G
Cuts at: 4005 4005
Size: 6406
MluI A'CGCG_T
Cuts at: 6282 6282
Size: 6406
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5520 5704 1305
Size: 4215 184 2007
Fragments arranged by size:
4215 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2826 10
Size: 54 1425 343 994 3590
Fragments arranged by size:
3590 1425 994 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2066 2642 3554 4364 4723 4882 518
Size: 648 900 576 912 810 359 159 2042
Fragments arranged by size:
2042 912 900 810 648 576 359 159
MspA1I CmG'CkG
Cuts at: 1606 2056 2543 3442 3822 4499 5647 5892 1606
Size: 450 487 899 380 677 1148 245 2120
Fragments arranged by size:
2120 1148 899 677 487 450 380 245
NaeI GCC'GGC
Cuts at: 2833 3539 3574 2833
Size: 706 35 5665
Fragments arranged by size:
5665 706 35
NcoI C'CATG_G
Cuts at: 513 1065 2037 3039 3526 513
Size: 552 972 1002 487 3393
Fragments arranged by size:
3393 1002 972 552 487
NdeI CA'TA_TG
Cuts at: 387 2359 387
Size: 1972 4434
NgoMIV G'CCGG_C
Cuts at: 2831 3537 3572 2831
Size: 706 35 5665
Fragments arranged by size:
5665 706 35
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6406
NotI GC'GGCC_GC
Cuts at: 3853 3853
Size: 6406
NspI r_CATG'y
Cuts at: 1132 1229 3055 3764 5309 1132
Size: 97 1826 709 1545 2229
Fragments arranged by size:
2229 1826 1545 709 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6406
PciI A'CATG_T
Cuts at: 3051 5305 3051
Size: 2254 4152
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2564 2725 1108
Size: 1456 161 4789
Fragments arranged by size:
4789 1456 161
PmeI GTTT'AAAC
Cuts at: 3710 3710
Size: 6406
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4475 4507 5988 6020 4475
Size: 32 1481 32 4861
Fragments arranged by size:
4861 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6406
PsiI TTA'TAA
Cuts at: 3976 3976
Size: 6406
PstI C_TGCA'G
Cuts at: 830 3157 3270 3414 3758 830
Size: 2327 113 144 344 3478
Fragments arranged by size:
3478 2327 344 144 113
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6406
PvuII CAG'CTG
Cuts at: 2056 2543 3442 2056
Size: 487 899 5020
Fragments arranged by size:
5020 899 487
SalI G'TCGA_C
Cuts at: 1936 3748 1936
Size: 1812 4594
SapI GCTCTTCn'nnn_
Cuts at: 3441 3468 3441
Size: 27 6379
SbfI CC_TGCA'GG
Cuts at: 3270 3414 3758 3270
Size: 144 344 5918
Fragments arranged by size:
5918 344 144
ScaI AGT'ACT
Cuts at: 4654 6366 4654
Size: 1712 4694
SfcI C'TryA_G
Cuts at: 826 1045 3153 3266 3410 3754 4889 5570 5761
Size: 219 2108 113 144 344 1135 681 191
Cuts at: 5761 826
Size: 1471
Fragments arranged by size:
2108 1471 1135 681 344 219 191 144
113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6406
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6406
SmaI CCC'GGG
Cuts at: 1872 3733 1872
Size: 1861 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3190 4510 5411 5673 820
Size: 197 926 1247 1320 901 262 1553
Fragments arranged by size:
1553 1320 1247 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4197 493
Size: 3704 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6406
SphI G_CATG'C
Cuts at: 1132 3764 1132
Size: 2632 3774
SspI AAT'ATT
Cuts at: 5 52 4330 5
Size: 47 4278 2081
Fragments arranged by size:
4278 2081 47
StuI AGG'CCT
Cuts at: 1517 3305 3638 1517
Size: 1788 333 4285
Fragments arranged by size:
4285 1788 333
StyI C'CwwG_G
Cuts at: 513 1065 2037 3039 3526 3845 513
Size: 552 972 1002 487 319 3074
Fragments arranged by size:
3074 1002 972 552 487 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3539 3602 4433 4450 4603 4788 5233
Size: 1255 1959 63 831 17 153 185 445
Cuts at: 5233 325
Size: 1498
Fragments arranged by size:
1959 1498 1255 831 445 185 153 63
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2134 4652 6364
Size: 274 80 33 51 1443 157 2518 1712
Cuts at: 6364 96
Size: 138
Fragments arranged by size:
2518 1712 1443 274 157 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2581 2613 3577 3609 6366 6398 2581
Size: 32 964 32 2757 32 2589
Fragments arranged by size:
2757 2589 964 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6406
XbaI T'CTAG_A
Cuts at: 3742 3742
Size: 6406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3056 3085 3056
Size: 29 6377
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6406
XmaI C'CCGG_G
Cuts at: 1870 3731 1870
Size: 1861 4545
XmnI GAAnn'nnTTC
Cuts at: 2714 4535 2714
Size: 1821 4585
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4214 276
Size: 53 83 186 936 2680 2468
Fragments arranged by size:
2680 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EarI EciI Eco57MI EcoRI EcoRV FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PpiI
PshAI PsiI PstI PvuI PvuII SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BfrBI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I
FalI FspAI KasI NarI NruI NsiI PacI PfoI
PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII
SanDI SexAI SfiI SfoI SrfI SwaI