Restriction map for
pFN21AB9925
(Circular) (Six-base) MAPSORT of: pFN21AB9925.seq Check: 5332 from: 1 to: 5743
pFN21AB9925 5743 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3553 278
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3086 1937
Size: 1149 4594
Acc65I G'GTAC_C
Cuts at: 3064 3064
Size: 5743
AclI AA'CG_TT
Cuts at: 1563 3870 4243 1563
Size: 2307 373 3063
Fragments arranged by size:
3063 2307 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2387 2468 3806 5190 5458 1632
Size: 755 81 1338 1384 268 1917
Fragments arranged by size:
1917 1384 1338 755 268 81
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5546
AflIII A'CryG_T
Cuts at: 1203 2913 4642 5619 1203
Size: 1710 1729 977 1327
Fragments arranged by size:
1729 1710 1327 977
AgeI A'CCGG_T
Cuts at: 4574 4574
Size: 5743
AhdI GACnn_n'nnGTC
Cuts at: 4472 4472
Size: 5743
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5743
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5743
AlwNI CAG_nnn'CTG
Cuts at: 2783 3486 5058 2783
Size: 703 1572 3468
Fragments arranged by size:
3468 1572 703
ApaLI G'TGCA_C
Cuts at: 3800 4956 3800
Size: 1156 4587
ApoI r'AATT_y
Cuts at: 1493 2548 2682 3052 3278 3430 5539 1493
Size: 1055 134 370 226 152 2109 1697
Fragments arranged by size:
2109 1697 1055 370 226 152 134
AseI AT'TA_AT
Cuts at: 160 1032 4297 160
Size: 872 3265 1606
Fragments arranged by size:
3265 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
AvaI C'yCGr_G
Cuts at: 1870 1943 2016 2760 3068 1870
Size: 73 73 744 308 4545
Fragments arranged by size:
4545 744 308 73 73
BamHI G'GATC_C
Cuts at: 3073 3440 3073
Size: 367 5376
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3064 4519 618
Size: 325 217 627 1277 1455 1842
Fragments arranged by size:
1842 1455 1277 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2853 3062 1391
Size: 1462 209 4072
Fragments arranged by size:
4072 1462 209
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2579 928
Size: 971 680 4092
Fragments arranged by size:
4092 971 680
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2729 2763 3934 3968 1903
Size: 34 792 34 1171 34 3678
Fragments arranged by size:
3678 1171 792 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3635 4845 3635
Size: 1210 4533
BclI T'GATC_A
Cuts at: 1548 2428 1548
Size: 880 4863
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4354 136
Size: 107 122 71 631 605 2682 1525
Fragments arranged by size:
2682 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2183 2886 5738 2183
Size: 703 2852 2188
Fragments arranged by size:
2852 2188 703
BlpI GC'TnA_GC
Cuts at: 3160 3160
Size: 5743
Bme1580I G_kGCm'C
Cuts at: 1790 2395 3804 4960 1790
Size: 605 1409 1156 2573
Fragments arranged by size:
2573 1409 1156 605
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5743
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2130 3474 4432
Size: 416 53 83 399 24 713 1344 958
Cuts at: 4432 442
Size: 1753
Fragments arranged by size:
1753 1344 958 713 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5743
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2867 3027 4403 1239
Size: 1628 160 1376 2579
Fragments arranged by size:
2579 1628 1376 160
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1995 3868 4733 5031 1995
Size: 1873 865 298 2707
Fragments arranged by size:
2707 1873 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2495 4406 882
Size: 617 996 1911 2219
Fragments arranged by size:
2219 1911 996 617
BsaAI yAC'GTr
Cuts at: 493 1204 3534 493
Size: 711 2330 2702
Fragments arranged by size:
2702 2330 711
BsaBI GATnn'nnATC
Cuts at: 5737 5737
Size: 5743
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3550 3932 4567 275
Size: 53 83 186 936 2017 382 635 1451
Fragments arranged by size:
2017 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4176 4574 4848 4995 5406 4176
Size: 398 274 147 411 4513
Fragments arranged by size:
4513 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3000 3030 1178
Size: 30 165 30 1597 30 3891
Fragments arranged by size:
3891 1597 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2099 2130 2917 1186
Size: 913 31 787 4012
Fragments arranged by size:
4012 913 787 31
BseYI C'CCAG_C
Cuts at: 4946 5442 4946
Size: 496 5247
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2210 2577 2781 1163
Size: 573 474 367 204 4125
Fragments arranged by size:
4125 573 474 367 204
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3193 3954 4982 1540
Size: 275 186 1192 761 1028 2301
Fragments arranged by size:
2301 1192 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2853 3038 3804 3889 4960 2853
Size: 185 766 85 1071 3636
Fragments arranged by size:
3636 1071 766 185 85
BsmI GAATG_Cn'
Cuts at: 2750 3254 3347 2750
Size: 504 93 5146
Fragments arranged by size:
5146 504 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2395 2853 3038 3062 3804 3889 4960
Size: 399 605 458 185 24 742 85 1071
Cuts at: 4960 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 605 458 399 185 85
24
BspEI T'CCGG_A
Cuts at: 5406 5406
Size: 5743
BspHI T'CATG_A
Cuts at: 2250 3632 5362 2250
Size: 1382 1730 2631
Fragments arranged by size:
2631 1730 1382
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3098 844
Size: 2254 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3630 1394
Size: 429 1807 3507
Fragments arranged by size:
3507 1807 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2868 4238 4412 65
Size: 1183 1620 1370 174 1396
Fragments arranged by size:
1620 1396 1370 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4387 4574 1700
Size: 110 2577 187 2869
Fragments arranged by size:
2869 2577 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5743
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5743
BssSI C'ACGA_G
Cuts at: 3803 4815 3803
Size: 1012 4731
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2458 794
Size: 1664 4079
BstBI TT'CG_AA
Cuts at: 4547 4547
Size: 5743
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5743
BstYI r'GATC_y
Cuts at: 1972 2183 2886 3073 3440 3825 3842 5294 5380
Size: 211 703 187 367 385 17 1452 86
Cuts at: 5380 5392 5738 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 703 385 367 346 211 187
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5004
Fragments arranged by size:
5004 683 56
BtsI GCAGTG_nn'
Cuts at: 2040 2307 2395 3259 4053 4073 2040
Size: 267 88 864 794 20 3710
Fragments arranged by size:
3710 864 794 267 88 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5743
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5708
DraI TTT'AAA
Cuts at: 3047 3047
Size: 5743
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4750 809
Size: 507 3434 1802
Fragments arranged by size:
3434 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3190 4079 5708
Size: 54 1425 50 275 18 1360 889 1629
Cuts at: 5708 8
Size: 43
Fragments arranged by size:
1629 1425 1360 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3190 1537
Size: 275 1378 4090
Fragments arranged by size:
4090 1378 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2959 3673 1353
Size: 1606 714 3423
Fragments arranged by size:
3423 1606 714
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4330 4704 4850 387
Size: 1064 138 2741 374 146 1280
Fragments arranged by size:
2741 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2851 2851
Size: 5743
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2387 2468 2867 3027 3806 4403 5190
Size: 393 755 81 399 160 779 597 787
Cuts at: 5190 5458 1239
Size: 268 1524
Fragments arranged by size:
1524 787 779 755 597 399 393 268
160 81
EcoRI G'AATT_C
Cuts at: 3052 3430 3052
Size: 378 5365
EcoRV GAT'ATC
Cuts at: 2311 2311
Size: 5743
FspI TGC'GCA
Cuts at: 4249 4249
Size: 5743
HaeII r_GCGC'y
Cuts at: 1678 4890 1678
Size: 3212 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3000 3032 4384 4416 4458
Size: 32 135 32 1428 32 1352 32 42
Cuts at: 4458 4490 1373
Size: 32 2626
Fragments arranged by size:
2626 1428 1352 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2832 3087 3333 669
Size: 988 281 894 255 246 3079
Fragments arranged by size:
3079 988 894 281 255 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5743
HpaI GTT'AAC
Cuts at: 3333 3333
Size: 5743
KpnI G_GTAC'C
Cuts at: 3068 3068
Size: 5743
MfeI C'AATT_G
Cuts at: 3342 3342
Size: 5743
MluI A'CGCG_T
Cuts at: 5619 5619
Size: 5743
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4857 5041 1305
Size: 3552 184 2007
Fragments arranged by size:
3552 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3921
Fragments arranged by size:
3921 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2805 2918 3701 4060 4219 518
Size: 648 1639 113 783 359 159 2042
Fragments arranged by size:
2042 1639 783 648 359 159 113
MspA1I CmG'CkG
Cuts at: 1606 3159 3836 4984 5229 1606
Size: 1553 677 1148 245 2120
Fragments arranged by size:
2120 1553 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5191
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5743
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5743
NotI GC'GGCC_GC
Cuts at: 3190 3190
Size: 5743
NspI r_CATG'y
Cuts at: 1132 1229 2917 3101 4646 1132
Size: 97 1688 184 1545 2229
Fragments arranged by size:
2229 1688 1545 184 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5743
PciI A'CATG_T
Cuts at: 2913 4642 2913
Size: 1729 4014
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5743
PmeI GTTT'AAAC
Cuts at: 3047 3047
Size: 5743
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3812 3844 5325 5357 3812
Size: 32 1481 32 4198
Fragments arranged by size:
4198 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5743
PsiI TTA'TAA
Cuts at: 3313 3313
Size: 5743
PstI C_TGCA'G
Cuts at: 830 2035 2332 2390 2463 3095 830
Size: 1205 297 58 73 632 3478
Fragments arranged by size:
3478 1205 632 297 73 58
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5743
SacI G_AGCT'C
Cuts at: 2853 2853
Size: 5743
SalI G'TCGA_C
Cuts at: 1936 3085 1936
Size: 1149 4594
SbfI CC_TGCA'GG
Cuts at: 3095 3095
Size: 5743
ScaI AGT'ACT
Cuts at: 3991 5703 3991
Size: 1712 4031
SfcI C'TryA_G
Cuts at: 826 1045 2031 2107 2328 2386 2459 3091 4226
Size: 219 986 76 221 58 73 632 1135
Cuts at: 4226 4907 5098 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 986 681 632 221 219 191
76 73 58
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5743
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5743
SmaI CCC'GGG
Cuts at: 1872 2018 3070 1872
Size: 146 1052 4545
Fragments arranged by size:
4545 1052 146
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2010 3847 4748 5010 820
Size: 197 926 67 1837 901 262 1553
Fragments arranged by size:
1837 1553 926 901 262 197 67
SnaBI TAC'GTA
Cuts at: 493 3534 493
Size: 3041 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5743
SphI G_CATG'C
Cuts at: 1132 3101 1132
Size: 1969 3774
SspI AAT'ATT
Cuts at: 5 52 2789 2945 3667 5
Size: 47 2737 156 722 2081
Fragments arranged by size:
2737 2081 722 156 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5743
StyI C'CwwG_G
Cuts at: 513 1065 3182 513
Size: 552 2117 3074
Fragments arranged by size:
3074 2117 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2280 3770 3787 3940 4125 4570 325
Size: 1255 700 1490 17 153 185 445 1498
Fragments arranged by size:
1498 1490 1255 700 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2103 3989 5701
Size: 274 80 33 51 1443 126 1886 1712
Cuts at: 5701 96
Size: 138
Fragments arranged by size:
1886 1712 1443 274 138 126 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5703 5735 5703
Size: 32 5711
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5743
XbaI T'CTAG_A
Cuts at: 3079 3079
Size: 5743
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5743
XmaI C'CCGG_G
Cuts at: 1870 2016 3068 1870
Size: 146 1052 4545
Fragments arranged by size:
4545 1052 146
XmnI GAAnn'nnTTC
Cuts at: 2381 3872 2381
Size: 1491 4252
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3551 276
Size: 53 83 186 936 2017 2468
Fragments arranged by size:
2468 2017 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PstI PvuI SacI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I
Bsu36I DraIII EcoNI EcoO109I FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI