Restriction map for
pFN21AB9918
(Circular) (Six-base) MAPSORT of: pFN21AB9918.seq Check: 3874 from: 1 to: 6442
pFN21AB9918 6442 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2640 4252 278
Size: 53 83 186 936 1104 1612 2468
Fragments arranged by size:
2468 1612 1104 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2009 3785 1937
Size: 72 1776 4594
Fragments arranged by size:
4594 1776 72
Acc65I G'GTAC_C
Cuts at: 3763 3763
Size: 6442
AclI AA'CG_TT
Cuts at: 1563 4569 4942 1563
Size: 3006 373 3063
Fragments arranged by size:
3063 3006 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2346 2619 2626 3723 4505 5889 6157 1632
Size: 714 273 7 1097 782 1384 268 1917
Fragments arranged by size:
1917 1384 1097 782 714 273 268 7
AfeI AGC'GCT
Cuts at: 2457 3044 2457
Size: 587 5855
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6245
AflIII A'CryG_T
Cuts at: 1203 2675 3177 5341 6318 1203
Size: 1472 502 2164 977 1327
Fragments arranged by size:
2164 1472 1327 977 502
AgeI A'CCGG_T
Cuts at: 5273 5273
Size: 6442
AhdI GACnn_n'nnGTC
Cuts at: 5171 5171
Size: 6442
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6442
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3036 1184
Size: 1852 4590
AlwNI CAG_nnn'CTG
Cuts at: 2018 3272 4185 5757 2018
Size: 1254 913 1572 2703
Fragments arranged by size:
2703 1572 1254 913
ApaLI G'TGCA_C
Cuts at: 4499 5655 4499
Size: 1156 5286
ApoI r'AATT_y
Cuts at: 1493 2520 2764 3751 3977 4129 6238 1493
Size: 1027 244 987 226 152 2109 1697
Fragments arranged by size:
2109 1697 1027 987 244 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4996 160
Size: 872 3964 1606
Fragments arranged by size:
3964 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
AvaI C'yCGr_G
Cuts at: 1870 1943 3767 1870
Size: 73 1824 4545
Fragments arranged by size:
4545 1824 73
BamHI G'GATC_C
Cuts at: 3772 4139 3772
Size: 367 6075
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2593 3763 5218 618
Size: 325 217 627 806 1170 1455 1842
Fragments arranged by size:
1842 1455 1170 806 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2336 3761 1391
Size: 945 1425 4072
Fragments arranged by size:
4072 1425 945
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2034 2632 928
Size: 971 135 598 4738
Fragments arranged by size:
4738 971 598 135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4633 4667 1903
Size: 34 2696 34 3678
Fragments arranged by size:
3678 2696 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2913 4334 5544 2913
Size: 1421 1210 3811
Fragments arranged by size:
3811 1421 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6442
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5053 136
Size: 107 122 71 631 605 3381 1525
Fragments arranged by size:
3381 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6437 6437
Size: 6442
BlpI GC'TnA_GC
Cuts at: 3859 3859
Size: 6442
Bme1580I G_kGCm'C
Cuts at: 1790 3464 4503 5659 1790
Size: 1674 1039 1156 2573
Fragments arranged by size:
2573 1674 1156 1039
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6442
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3297 4173 5131
Size: 416 53 83 399 24 1880 876 958
Cuts at: 5131 442
Size: 1753
Fragments arranged by size:
1880 1753 958 876 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6442
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 5102 1239
Size: 3863 2579
Bpu10I CC'TnA_GC
Cuts at: 2772 2772
Size: 6442
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4567 5432 5730 4567
Size: 865 298 5279
Fragments arranged by size:
5279 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5105 882
Size: 617 3606 2219
Fragments arranged by size:
3606 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4233 493
Size: 711 3029 2702
Fragments arranged by size:
3029 2702 711
BsaBI GATnn'nnATC
Cuts at: 6436 6436
Size: 6442
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2637 4249 4631 5266
Size: 53 83 186 936 1104 1612 382 635
Cuts at: 5266 275
Size: 1451
Fragments arranged by size:
1612 1451 1104 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4875 5273 5547 5694 6105 4875
Size: 398 274 147 411 5212
Fragments arranged by size:
5212 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6217
Fragments arranged by size:
6217 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2017 2020 2023 2026 2151 1186
Size: 831 3 3 3 125 5477
Fragments arranged by size:
5477 831 125 3 3 3
BseYI C'CCAG_C
Cuts at: 2646 2707 2820 5645 6141 2646
Size: 61 113 2825 496 2947
Fragments arranged by size:
2947 2825 496 113 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5869
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2049 3892 4653 5681 1540
Size: 275 186 48 1843 761 1028 2301
Fragments arranged by size:
2301 1843 1028 761 275 186 48
BsiHKAI G_wGCw'C
Cuts at: 4503 4588 5659 4503
Size: 85 1071 5286
Fragments arranged by size:
5286 1071 85
BsmI GAATG_Cn'
Cuts at: 3953 4046 3953
Size: 93 6349
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2336 3464 3761 4503 4588 5659 1391
Size: 399 546 1128 297 742 85 1071 2174
Fragments arranged by size:
2174 1128 1071 742 546 399 297 85
BspEI T'CCGG_A
Cuts at: 6105 6105
Size: 6442
BspHI T'CATG_A
Cuts at: 4331 6061 4331
Size: 1730 4712
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3797 844
Size: 2953 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4329 1394
Size: 429 2506 3507
Fragments arranged by size:
3507 2506 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2366 2400 2967 3689 4937 5111 65
Size: 1183 1118 34 567 722 1248 174 1396
Fragments arranged by size:
1396 1248 1183 1118 722 567 174 34
BsrFI r'CCGG_y
Cuts at: 1700 1810 2048 5086 5273 1700
Size: 110 238 3038 187 2869
Fragments arranged by size:
3038 2869 238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6442
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6442
BssSI C'ACGA_G
Cuts at: 4502 5514 4502
Size: 1012 5430
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6442
BstBI TT'CG_AA
Cuts at: 5246 5246
Size: 6442
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6442
BstXI CCAn_nnnn'nTGG
Cuts at: 3415 3415
Size: 6442
BstYI r'GATC_y
Cuts at: 1972 2812 3236 3772 4139 4524 4541 5993 6079
Size: 840 424 536 367 385 17 1452 86
Cuts at: 6079 6091 6437 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 840 536 424 385 367 346
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3325 3325
Size: 6442
BtgI C'CryG_G
Cuts at: 513 1065 3118 3362 513
Size: 552 2053 244 3593
Fragments arranged by size:
3593 2053 552 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5703
Fragments arranged by size:
5703 683 56
BtsI GCAGTG_nn'
Cuts at: 3133 3492 3958 4752 4772 3133
Size: 359 466 794 20 4803
Fragments arranged by size:
4803 794 466 359 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6442
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6407
DraI TTT'AAA
Cuts at: 3746 3746
Size: 6442
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5449 809
Size: 507 4133 1802
Fragments arranged by size:
4133 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2046 3889 4778
Size: 54 1425 50 275 18 216 1843 889
Cuts at: 4778 6407 8
Size: 1629 43
Fragments arranged by size:
1843 1629 1425 889 275 216 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2046 3889 1537
Size: 275 234 1843 4090
Fragments arranged by size:
4090 1843 275 234
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 2115 2128 4372 1353
Size: 732 30 13 2244 3423
Fragments arranged by size:
3423 2244 732 30 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5029 5403 5549 387
Size: 1064 138 3440 374 146 1280
Fragments arranged by size:
3440 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2346 2619 2626 3723 4505 5102 5889
Size: 393 714 273 7 1097 782 597 787
Cuts at: 5889 6157 1239
Size: 268 1524
Fragments arranged by size:
1524 1097 787 782 714 597 393 273
268 7
EcoNI CCTnn'n_nnAGG
Cuts at: 3626 3626
Size: 6442
EcoO109I rG'GnC_Cy
Cuts at: 2077 2470 2077
Size: 393 6049
EcoRI G'AATT_C
Cuts at: 3751 4129 3751
Size: 378 6064
FspI TGC'GCA
Cuts at: 4948 4948
Size: 6442
HaeII r_GCGC'y
Cuts at: 1678 2459 2827 3046 5589 1678
Size: 781 368 219 2543 2531
Fragments arranged by size:
2543 2531 781 368 219
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2662 2694 5083 5115 5157
Size: 32 135 32 1090 32 2389 32 42
Cuts at: 5157 5189 1373
Size: 32 2626
Fragments arranged by size:
2626 2389 1090 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3786 4032 669
Size: 988 281 1848 246 3079
Fragments arranged by size:
3079 1848 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2276 3103 3641 748
Size: 1528 827 538 3549
Fragments arranged by size:
3549 1528 827 538
HpaI GTT'AAC
Cuts at: 4032 4032
Size: 6442
KpnI G_GTAC'C
Cuts at: 3767 3767
Size: 6442
MfeI C'AATT_G
Cuts at: 4041 4041
Size: 6442
MluI A'CGCG_T
Cuts at: 2675 6318 2675
Size: 3643 2799
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2110 2660 5556 5740 1305
Size: 805 550 2896 184 2007
Fragments arranged by size:
2896 2007 805 550 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4620
Fragments arranged by size:
4620 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3005 3413 4400 4759 4918 518
Size: 648 1839 408 987 359 159 2042
Fragments arranged by size:
2042 1839 987 648 408 359 159
MspA1I CmG'CkG
Cuts at: 1606 2646 3858 4535 5683 5928 1606
Size: 1040 1212 677 1148 245 2120
Fragments arranged by size:
2120 1212 1148 1040 677 245
NcoI C'CATG_G
Cuts at: 513 1065 3362 513
Size: 552 2297 3593
Fragments arranged by size:
3593 2297 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6442
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6442
NotI GC'GGCC_GC
Cuts at: 3889 3889
Size: 6442
NspI r_CATG'y
Cuts at: 1132 1229 2446 3181 3800 5345 1132
Size: 97 1217 735 619 1545 2229
Fragments arranged by size:
2229 1545 1217 735 619 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6442
PciI A'CATG_T
Cuts at: 3177 5341 3177
Size: 2164 4278
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2425 1108
Size: 1317 5125
PfoI T'CCnGG_A
Cuts at: 2878 2878
Size: 6442
PmeI GTTT'AAAC
Cuts at: 3746 3746
Size: 6442
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4511 4543 6024 6056 4511
Size: 32 1481 32 4897
Fragments arranged by size:
4897 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2077 2470 2077
Size: 393 6049
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6442
PsiI TTA'TAA
Cuts at: 4012 4012
Size: 6442
PstI C_TGCA'G
Cuts at: 830 3794 830
Size: 2964 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6442
PvuII CAG'CTG
Cuts at: 2646 2646
Size: 6442
SalI G'TCGA_C
Cuts at: 1936 3784 1936
Size: 1848 4594
SbfI CC_TGCA'GG
Cuts at: 3794 3794
Size: 6442
ScaI AGT'ACT
Cuts at: 4690 6402 4690
Size: 1712 4730
SexAI A'CCwGG_T
Cuts at: 3271 3271
Size: 6442
SfcI C'TryA_G
Cuts at: 826 1045 2010 3443 3790 4925 5606 5797 826
Size: 219 965 1433 347 1135 681 191 1471
Fragments arranged by size:
1471 1433 1135 965 681 347 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6442
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6442
SmaI CCC'GGG
Cuts at: 1872 3769 1872
Size: 1897 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4546 5447 5709 820
Size: 197 926 2603 901 262 1553
Fragments arranged by size:
2603 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4233 493
Size: 3740 2702
SpeI A'CTAG_T
Cuts at: 152 3571 152
Size: 3419 3023
SphI G_CATG'C
Cuts at: 1132 2446 3800 1132
Size: 1314 1354 3774
Fragments arranged by size:
3774 1354 1314
SspI AAT'ATT
Cuts at: 5 52 2269 4366 5
Size: 47 2217 2097 2081
Fragments arranged by size:
2217 2097 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6442
StyI C'CwwG_G
Cuts at: 513 1065 2419 3362 3881 513
Size: 552 1354 943 519 3074
Fragments arranged by size:
3074 1354 943 552 519
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4469 4486 4639 4824 5269 325
Size: 1255 2889 17 153 185 445 1498
Fragments arranged by size:
2889 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2948 4688 6400
Size: 274 80 33 51 1443 971 1740 1712
Cuts at: 6400 96
Size: 138
Fragments arranged by size:
1740 1712 1443 971 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6402 6434 6402
Size: 32 6410
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6442
XbaI T'CTAG_A
Cuts at: 3778 3778
Size: 6442
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3478 3478
Size: 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6442
XmaI C'CCGG_G
Cuts at: 1870 3767 1870
Size: 1897 4545
XmnI GAAnn'nnTTC
Cuts at: 4571 4571
Size: 6442
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2638 4250 276
Size: 53 83 186 936 1104 1612 2468
Fragments arranged by size:
2468 1612 1104 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SfoI SrfI SwaI