Restriction map for
pFN21AB9913
(Circular) (Six-base) MAPSORT of: pFN21AB9913.seq Check: 5619 from: 1 to: 5560
pFN21AB9913 5560 bp
With 182 enzymes: SgfI *
October 20, 2009 10:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3370 278
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2808 2903 1937
Size: 871 95 4594
Fragments arranged by size:
4594 871 95
Acc65I G'GTAC_C
Cuts at: 2881 2881
Size: 5560
AclI AA'CG_TT
Cuts at: 1563 3687 4060 1563
Size: 2124 373 3063
Fragments arranged by size:
3063 2124 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2817 3623 5007 5275 1632
Size: 1185 806 1384 268 1917
Fragments arranged by size:
1917 1384 1185 806 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5363
AflIII A'CryG_T
Cuts at: 1203 4459 5436 1203
Size: 3256 977 1327
Fragments arranged by size:
3256 1327 977
AgeI A'CCGG_T
Cuts at: 4391 4391
Size: 5560
AhdI GACnn_n'nnGTC
Cuts at: 4289 4289
Size: 5560
AleI CACnn'nnGTG
Cuts at: 1166 2798 1166
Size: 1632 3928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5560
AlwNI CAG_nnn'CTG
Cuts at: 3303 4875 3303
Size: 1572 3988
ApaLI G'TGCA_C
Cuts at: 2779 3617 4773 2779
Size: 838 1156 3566
Fragments arranged by size:
3566 1156 838
ApoI r'AATT_y
Cuts at: 1493 2183 2212 2869 3095 3247 5356 1493
Size: 690 29 657 226 152 2109 1697
Fragments arranged by size:
2109 1697 690 657 226 152 29
AseI AT'TA_AT
Cuts at: 160 1032 4114 160
Size: 872 3082 1606
Fragments arranged by size:
3082 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
AvaI C'yCGr_G
Cuts at: 1870 1943 2885 1870
Size: 73 942 4545
Fragments arranged by size:
4545 942 73
BamHI G'GATC_C
Cuts at: 2890 3257 2890
Size: 367 5193
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2881 4336 618
Size: 325 217 627 1094 1455 1842
Fragments arranged by size:
1842 1455 1094 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2879 1391
Size: 1488 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2352 928
Size: 971 453 4136
Fragments arranged by size:
4136 971 453
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3751 3785 1903
Size: 34 1814 34 3678
Fragments arranged by size:
3678 1814 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3452 4662 3452
Size: 1210 4350
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5560
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4171 136
Size: 107 122 71 631 605 2499 1525
Fragments arranged by size:
2499 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5555 5555
Size: 5560
BlpI GC'TnA_GC
Cuts at: 2977 2977
Size: 5560
Bme1580I G_kGCm'C
Cuts at: 1790 2783 3621 4777 1790
Size: 993 838 1156 2573
Fragments arranged by size:
2573 1156 993 838
BmgBI CAC'GTC
Cuts at: 1318 2504 1318
Size: 1186 4374
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2765 3291 4249
Size: 416 53 83 399 24 1348 526 958
Cuts at: 4249 442
Size: 1753
Fragments arranged by size:
1753 1348 958 526 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5560
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4220 1239
Size: 2981 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3685 4550 4848 3685
Size: 865 298 4397
Fragments arranged by size:
4397 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4223 882
Size: 617 2724 2219
Fragments arranged by size:
2724 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3351 493
Size: 711 2147 2702
Fragments arranged by size:
2702 2147 711
BsaBI GATnn'nnATC
Cuts at: 5554 5554
Size: 5560
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3367 3749 4384 275
Size: 53 83 186 936 1834 382 635 1451
Fragments arranged by size:
1834 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2017 2153 3993 4391 4665 4812 5223 2017
Size: 136 1840 398 274 147 411 2354
Fragments arranged by size:
2354 1840 411 398 274 147 136
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2225 2255 1178
Size: 30 165 30 822 30 4483
Fragments arranged by size:
4483 822 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2075 2663 1186
Size: 889 588 4083
Fragments arranged by size:
4083 889 588
BseYI C'CCAG_C
Cuts at: 4763 5259 4763
Size: 496 5064
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4987
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3010 3771 4799 1540
Size: 275 186 1009 761 1028 2301
Fragments arranged by size:
2301 1028 1009 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2783 3621 3706 4777 2783
Size: 838 85 1071 3566
Fragments arranged by size:
3566 1071 838 85
BsmI GAATG_Cn'
Cuts at: 3071 3164 3071
Size: 93 5467
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2783 2879 3621 3706 4777 1391
Size: 399 993 96 742 85 1071 2174
Fragments arranged by size:
2174 1071 993 742 399 96 85
BspEI T'CCGG_A
Cuts at: 2017 5223 2017
Size: 3206 2354
BspHI T'CATG_A
Cuts at: 3449 5179 3449
Size: 1730 3830
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2915 844
Size: 2071 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3447 1394
Size: 429 1624 3507
Fragments arranged by size:
3507 1624 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2622 4055 4229 65
Size: 1183 1374 1433 174 1396
Fragments arranged by size:
1433 1396 1374 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2010 2065 4204 4391 1700
Size: 110 200 55 2139 187 2869
Fragments arranged by size:
2869 2139 200 187 110 55
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5560
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5560
BssSI C'ACGA_G
Cuts at: 3620 4632 3620
Size: 1012 4548
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5560
BstBI TT'CG_AA
Cuts at: 4364 4364
Size: 5560
BstEII G'GTnAC_C
Cuts at: 1184 2362 2474 1184
Size: 1178 112 4270
Fragments arranged by size:
4270 1178 112
BstXI CCAn_nnnn'nTGG
Cuts at: 2800 2800
Size: 5560
BstYI r'GATC_y
Cuts at: 1972 2261 2890 3257 3642 3659 5111 5197 5209
Size: 289 629 367 385 17 1452 86 12
Cuts at: 5209 5555 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 629 385 367 346 289 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2269 2269
Size: 5560
BtgI C'CryG_G
Cuts at: 513 1065 2479 513
Size: 552 1414 3594
Fragments arranged by size:
3594 1414 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4821
Fragments arranged by size:
4821 683 56
BtsI GCAGTG_nn'
Cuts at: 3076 3870 3890 3076
Size: 794 20 4746
Fragments arranged by size:
4746 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5560
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5525
DraI TTT'AAA
Cuts at: 2864 2864
Size: 5560
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4567 809
Size: 507 3251 1802
Fragments arranged by size:
3251 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3007 3896 5525
Size: 54 1425 50 275 18 1177 889 1629
Cuts at: 5525 8
Size: 43
Fragments arranged by size:
1629 1425 1177 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3007 1537
Size: 275 1195 4090
Fragments arranged by size:
4090 1195 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2040 3490 1353
Size: 687 1450 3423
Fragments arranged by size:
3423 1450 687
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4147 4521 4667 387
Size: 1064 138 2558 374 146 1280
Fragments arranged by size:
2558 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2817 3623 4220 5007 5275 1239
Size: 393 1185 806 597 787 268 1524
Fragments arranged by size:
1524 1185 806 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2869 3247 2869
Size: 378 5182
FspI TGC'GCA
Cuts at: 4066 4066
Size: 5560
HaeII r_GCGC'y
Cuts at: 1678 4707 1678
Size: 3029 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2228 2260 2488 2520 4201
Size: 32 135 32 656 32 228 32 1681
Cuts at: 4201 4233 4275 4307 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1681 656 228 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2507 2904 3150 669
Size: 988 281 569 397 246 3079
Fragments arranged by size:
3079 988 569 397 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5560
HpaI GTT'AAC
Cuts at: 3150 3150
Size: 5560
KpnI G_GTAC'C
Cuts at: 2885 2885
Size: 5560
MfeI C'AATT_G
Cuts at: 3159 3159
Size: 5560
MluI A'CGCG_T
Cuts at: 5436 5436
Size: 5560
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2321 2423 4674 4858 1305
Size: 1016 102 2251 184 2007
Fragments arranged by size:
2251 2007 1016 184 102
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3738
Fragments arranged by size:
3738 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2798 3518 3877 4036 518
Size: 648 1632 720 359 159 2042
Fragments arranged by size:
2042 1632 720 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2976 3653 4801 5046 1606
Size: 1370 677 1148 245 2120
Fragments arranged by size:
2120 1370 1148 677 245
NaeI GCC'GGC
Cuts at: 2067 2067
Size: 5560
NcoI C'CATG_G
Cuts at: 513 1065 2479 513
Size: 552 1414 3594
Fragments arranged by size:
3594 1414 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5560
NgoMIV G'CCGG_C
Cuts at: 2065 2065
Size: 5560
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5560
NotI GC'GGCC_GC
Cuts at: 3007 3007
Size: 5560
NspI r_CATG'y
Cuts at: 1132 1229 2918 4463 1132
Size: 97 1689 1545 2229
Fragments arranged by size:
2229 1689 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5560
PciI A'CATG_T
Cuts at: 4459 4459
Size: 5560
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2485 1108
Size: 1377 4183
PmeI GTTT'AAAC
Cuts at: 2864 2864
Size: 5560
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3629 3661 5142 5174 3629
Size: 32 1481 32 4015
Fragments arranged by size:
4015 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5560
PsiI TTA'TAA
Cuts at: 3130 3130
Size: 5560
PstI C_TGCA'G
Cuts at: 830 2912 830
Size: 2082 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5560
SalI G'TCGA_C
Cuts at: 1936 2902 1936
Size: 966 4594
SapI GCTCTTCn'nnn_
Cuts at: 2040 2040
Size: 5560
SbfI CC_TGCA'GG
Cuts at: 2912 2912
Size: 5560
ScaI AGT'ACT
Cuts at: 3808 5520 3808
Size: 1712 3848
SfcI C'TryA_G
Cuts at: 826 1045 2908 4043 4724 4915 826
Size: 219 1863 1135 681 191 1471
Fragments arranged by size:
1863 1471 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5560
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5560
SmaI CCC'GGG
Cuts at: 1872 2887 1872
Size: 1015 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3664 4565 4827 820
Size: 197 926 1721 901 262 1553
Fragments arranged by size:
1721 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3351 493
Size: 2858 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5560
SphI G_CATG'C
Cuts at: 1132 2918 1132
Size: 1786 3774
SspI AAT'ATT
Cuts at: 5 52 3484 5
Size: 47 3432 2081
Fragments arranged by size:
3432 2081 47
StuI AGG'CCT
Cuts at: 1517 2450 1517
Size: 933 4627
StyI C'CwwG_G
Cuts at: 513 1065 2479 2999 513
Size: 552 1414 520 3074
Fragments arranged by size:
3074 1414 552 520
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2784 3587 3604 3757 3942 4387 325
Size: 1255 1204 803 17 153 185 445 1498
Fragments arranged by size:
1498 1255 1204 803 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2356 3806 5518
Size: 274 80 33 51 1443 379 1450 1712
Cuts at: 5518 96
Size: 138
Fragments arranged by size:
1712 1450 1443 379 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5520 5552 5520
Size: 32 5528
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5560
XbaI T'CTAG_A
Cuts at: 2896 2896
Size: 5560
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2227 2227
Size: 5560
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5560
XmaI C'CCGG_G
Cuts at: 1870 2885 1870
Size: 1015 4545
XmnI GAAnn'nnTTC
Cuts at: 2826 3689 2826
Size: 863 4697
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3368 276
Size: 53 83 186 936 1834 2468
Fragments arranged by size:
2468 1834 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI
SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI
SfiI SfoI SrfI SwaI