Restriction map for
pFN21AB9897
(Circular) (Six-base) MAPSORT of: pFN21AB9897.seq Check: 8869 from: 1 to: 6235
pFN21AB9897 6235 bp
With 182 enzymes: SgfI *
January 30, 2014 17:24 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4045 278
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2774 3578 1937
Size: 837 804 4594
Fragments arranged by size:
4594 837 804
Acc65I G'GTAC_C
Cuts at: 3556 3556
Size: 6235
AclI AA'CG_TT
Cuts at: 1563 4362 4735 1563
Size: 2799 373 3063
Fragments arranged by size:
3063 2799 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2265 2477 2637 4298 5682 5950 1632
Size: 633 212 160 1661 1384 268 1917
Fragments arranged by size:
1917 1661 1384 633 268 212 160
AflII C'TTAA_G
Cuts at: 820 1017 3125 820
Size: 197 2108 3930
Fragments arranged by size:
3930 2108 197
AflIII A'CryG_T
Cuts at: 1203 2558 3530 5134 6111 1203
Size: 1355 972 1604 977 1327
Fragments arranged by size:
1604 1355 1327 977 972
AgeI A'CCGG_T
Cuts at: 5066 5066
Size: 6235
AhdI GACnn_n'nnGTC
Cuts at: 2533 4964 2533
Size: 2431 3804
AleI CACnn'nnGTG
Cuts at: 1166 3531 1166
Size: 2365 3870
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6235
AlwNI CAG_nnn'CTG
Cuts at: 3978 5550 3978
Size: 1572 4663
ApaLI G'TGCA_C
Cuts at: 4292 5448 4292
Size: 1156 5079
ApoI r'AATT_y
Cuts at: 1493 2156 2275 2650 2981 3544 3770 3922 6031
Size: 663 119 375 331 563 226 152 2109
Cuts at: 6031 1493
Size: 1697
Fragments arranged by size:
2109 1697 663 563 375 331 226 152
119
AseI AT'TA_AT
Cuts at: 160 1032 3110 4789 160
Size: 872 2078 1679 1606
Fragments arranged by size:
2078 1679 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
AvaI C'yCGr_G
Cuts at: 1870 1943 3560 1870
Size: 73 1617 4545
Fragments arranged by size:
4545 1617 73
BamHI G'GATC_C
Cuts at: 3565 3932 3565
Size: 367 5868
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2734 3556 5011 618
Size: 325 217 627 947 822 1455 1842
Fragments arranged by size:
1842 1455 947 822 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3251 3554 1391
Size: 1860 303 4072
Fragments arranged by size:
4072 1860 303
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5264
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4426 4460 1903
Size: 34 2489 34 3678
Fragments arranged by size:
3678 2489 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4127 5337 4127
Size: 1210 5025
BclI T'GATC_A
Cuts at: 1548 2827 1548
Size: 1279 4956
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4846 136
Size: 107 122 71 631 605 3174 1525
Fragments arranged by size:
3174 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2726 3121 6230 2726
Size: 395 3109 2731
Fragments arranged by size:
3109 2731 395
BlpI GC'TnA_GC
Cuts at: 3652 3652
Size: 6235
Bme1580I G_kGCm'C
Cuts at: 1790 4296 5452 1790
Size: 2506 1156 2573
Fragments arranged by size:
2573 2506 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6235
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3966 4924 442
Size: 416 53 83 399 24 2549 958 1753
Fragments arranged by size:
2549 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4895 1239
Size: 3656 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2924 4360 5225 5523 2924
Size: 1436 865 298 3636
Fragments arranged by size:
3636 1436 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2702 2879 4898 882
Size: 617 1203 177 2019 2219
Fragments arranged by size:
2219 2019 1203 617 177
BsaAI yAC'GTr
Cuts at: 493 1204 2559 4026 493
Size: 711 1355 1467 2702
Fragments arranged by size:
2702 1467 1355 711
BsaBI GATnn'nnATC
Cuts at: 2877 2919 6229 2877
Size: 42 3310 2883
Fragments arranged by size:
3310 2883 42
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4042 4424 5059 275
Size: 53 83 186 936 2509 382 635 1451
Fragments arranged by size:
2509 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4668 5066 5340 5487 5898 4668
Size: 398 274 147 411 5005
Fragments arranged by size:
5005 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6010
Fragments arranged by size:
6010 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6235
BseYI C'CCAG_C
Cuts at: 5438 5934 5438
Size: 496 5739
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5662
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3685 4446 5474 1540
Size: 275 186 1684 761 1028 2301
Fragments arranged by size:
2301 1684 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 4296 4381 5452 4296
Size: 85 1071 5079
Fragments arranged by size:
5079 1071 85
BsmI GAATG_Cn'
Cuts at: 3746 3839 3746
Size: 93 6142
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3251 3554 4296 4381 5452 1391
Size: 399 1461 303 742 85 1071 2174
Fragments arranged by size:
2174 1461 1071 742 399 303 85
BspEI T'CCGG_A
Cuts at: 5898 5898
Size: 6235
BspHI T'CATG_A
Cuts at: 4124 5854 4124
Size: 1730 4505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2015 3590 844
Size: 1171 1575 3489
Fragments arranged by size:
3489 1575 1171
BsrBI CCG'CTC
Cuts at: 1394 1823 4122 1394
Size: 429 2299 3507
Fragments arranged by size:
3507 2299 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4730 4904 65
Size: 1183 3482 174 1396
Fragments arranged by size:
3482 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4879 5066 1700
Size: 110 3069 187 2869
Fragments arranged by size:
3069 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6235
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6235
BssSI C'ACGA_G
Cuts at: 4295 5307 4295
Size: 1012 5223
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2198 794
Size: 1404 4831
BstBI TT'CG_AA
Cuts at: 5039 5039
Size: 6235
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6235
BstYI r'GATC_y
Cuts at: 1972 2726 3121 3565 3932 4317 4334 5786 5872
Size: 754 395 444 367 385 17 1452 86
Cuts at: 5872 5884 6230 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 754 444 395 385 367 346
86 17 12
BstZ17I GTA'TAC
Cuts at: 2775 2775
Size: 6235
Bsu36I CC'TnA_GG
Cuts at: 3450 3450
Size: 6235
BtgI C'CryG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 4453
Fragments arranged by size:
4453 939 552 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5496
Fragments arranged by size:
5496 683 56
BtsI GCAGTG_nn'
Cuts at: 2917 3339 3751 4545 4565 2917
Size: 422 412 794 20 4587
Fragments arranged by size:
4587 794 422 412 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6235
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3066 3101 529
Size: 35 2502 35 3663
Fragments arranged by size:
3663 2502 35 35
DraI TTT'AAA
Cuts at: 3539 3539
Size: 6235
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5242 809
Size: 507 3926 1802
Fragments arranged by size:
3926 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2472 3682 4571
Size: 54 1425 50 275 18 642 1210 889
Cuts at: 4571 6200 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1210 889 642 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3682 1537
Size: 275 1870 4090
Fragments arranged by size:
4090 1870 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2146 3019 3426 4165 1353
Size: 793 873 407 739 3423
Fragments arranged by size:
3423 873 793 739 407
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2810 4822 5196 5342 387
Size: 1064 138 1221 2012 374 146 1280
Fragments arranged by size:
2012 1280 1221 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2265 2477 2637 4298 4895 5682 5950
Size: 393 633 212 160 1661 597 787 268
Cuts at: 5950 1239
Size: 1524
Fragments arranged by size:
1661 1524 787 633 597 393 268 212
160
EcoNI CCTnn'n_nnAGG
Cuts at: 2100 2100
Size: 6235
EcoO109I rG'GnC_Cy
Cuts at: 2105 2997 3483 2105
Size: 892 486 4857
Fragments arranged by size:
4857 892 486
EcoRI G'AATT_C
Cuts at: 2275 2981 3544 3922 2275
Size: 706 563 378 4588
Fragments arranged by size:
4588 706 563 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2595 2595
Size: 6235
FspI TGC'GCA
Cuts at: 4741 4741
Size: 6235
HaeII r_GCGC'y
Cuts at: 1678 5382 1678
Size: 3704 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2519 2551 4876 4908 4950
Size: 32 135 32 947 32 2325 32 42
Cuts at: 4950 4982 1373
Size: 32 2626
Fragments arranged by size:
2626 2325 947 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3579 3825 669
Size: 988 281 1641 246 3079
Fragments arranged by size:
3079 1641 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6235
HpaI GTT'AAC
Cuts at: 3825 3825
Size: 6235
KpnI G_GTAC'C
Cuts at: 3560 3560
Size: 6235
MfeI C'AATT_G
Cuts at: 2468 3834 2468
Size: 1366 4869
MluI A'CGCG_T
Cuts at: 6111 6111
Size: 6235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5349 5533 1305
Size: 4044 184 2007
Fragments arranged by size:
4044 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4413
Fragments arranged by size:
4413 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2850 2975 3333 3531 4193 4552 4711
Size: 648 1684 125 358 198 662 359 159
Cuts at: 4711 518
Size: 2042
Fragments arranged by size:
2042 1684 662 648 359 358 198 159
125
MspA1I CmG'CkG
Cuts at: 1606 3651 4328 5476 5721 1606
Size: 2045 677 1148 245 2120
Fragments arranged by size:
2120 2045 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2295 513
Size: 552 939 291 4453
Fragments arranged by size:
4453 939 552 291
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6235
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6235
NotI GC'GGCC_GC
Cuts at: 3682 3682
Size: 6235
NspI r_CATG'y
Cuts at: 1132 1229 3534 3593 5138 1132
Size: 97 2305 59 1545 2229
Fragments arranged by size:
2305 2229 1545 97 59
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6235
PciI A'CATG_T
Cuts at: 3530 5134 3530
Size: 1604 4631
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6235
PmeI GTTT'AAAC
Cuts at: 3539 3539
Size: 6235
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4304 4336 5817 5849 4304
Size: 32 1481 32 4690
Fragments arranged by size:
4690 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2105 2997 3483 2105
Size: 892 486 4857
Fragments arranged by size:
4857 892 486
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6235
PsiI TTA'TAA
Cuts at: 2141 2403 3805 2141
Size: 262 1402 4571
Fragments arranged by size:
4571 1402 262
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2826 2858 2826
Size: 32 6203
PstI C_TGCA'G
Cuts at: 830 3587 830
Size: 2757 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6235
SalI G'TCGA_C
Cuts at: 1936 3577 1936
Size: 1641 4594
SbfI CC_TGCA'GG
Cuts at: 3587 3587
Size: 6235
ScaI AGT'ACT
Cuts at: 2076 4483 6195 2076
Size: 2407 1712 2116
Fragments arranged by size:
2407 2116 1712
SfcI C'TryA_G
Cuts at: 826 1045 3425 3583 4718 5399 5590 826
Size: 219 2380 158 1135 681 191 1471
Fragments arranged by size:
2380 1471 1135 681 219 191 158
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6235
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6235
SmaI CCC'GGG
Cuts at: 1872 3562 1872
Size: 1690 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2903 3125 4339 5240 5502 820
Size: 197 926 960 222 1214 901 262 1553
Fragments arranged by size:
1553 1214 960 926 901 262 222 197
SnaBI TAC'GTA
Cuts at: 493 4026 493
Size: 3533 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6235
SphI G_CATG'C
Cuts at: 1132 3593 1132
Size: 2461 3774
SspI AAT'ATT
Cuts at: 5 52 2839 4159 5
Size: 47 2787 1320 2081
Fragments arranged by size:
2787 2081 1320 47
StuI AGG'CCT
Cuts at: 1517 2313 1517
Size: 796 5439
StyI C'CwwG_G
Cuts at: 513 1065 2004 2295 3000 3674 513
Size: 552 939 291 705 674 3074
Fragments arranged by size:
3074 939 705 674 552 291
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2752 4262 4279 4432 4617 5062 325
Size: 1255 1172 1510 17 153 185 445 1498
Fragments arranged by size:
1510 1498 1255 1172 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2074 4481 6193
Size: 274 80 33 51 1443 97 2407 1712
Cuts at: 6193 96
Size: 138
Fragments arranged by size:
2407 1712 1443 274 138 97 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3270 3302 6195 6227 3270
Size: 32 2893 32 3278
Fragments arranged by size:
3278 2893 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6235
XbaI T'CTAG_A
Cuts at: 3571 3571
Size: 6235
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6235
XmaI C'CCGG_G
Cuts at: 1870 3560 1870
Size: 1690 4545
XmnI GAAnn'nnTTC
Cuts at: 2394 3324 4364 2394
Size: 930 1040 4265
Fragments arranged by size:
4265 1040 930
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4043 276
Size: 53 83 186 936 2509 2468
Fragments arranged by size:
2509 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI DraIII
EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PspOMI PspXI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI XcmI