Restriction map for
pFN21AB9896
(Circular) (Six-base) MAPSORT of: pFN21AB9896.seq Check: 8111 from: 1 to: 8236
pFN21AB9896 8236 bp
With 182 enzymes: SgfI *
March 2, 2011 15:47 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 4005 4812 4005
Size: 807 7429
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 6046 278
Size: 53 83 186 936 4510 2468
Fragments arranged by size:
4510 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3657 3792 4110 4701 5579 1937
Size: 1720 135 318 591 878 4594
Fragments arranged by size:
4594 1720 878 591 318 135
Acc65I G'GTAC_C
Cuts at: 4608 4944 5557 4608
Size: 336 613 7287
Fragments arranged by size:
7287 613 336
AclI AA'CG_TT
Cuts at: 1563 6363 6736 1563
Size: 4800 373 3063
Fragments arranged by size:
4800 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2005 2177 3305 6299 7683 7951 1632
Size: 373 172 1128 2994 1384 268 1917
Fragments arranged by size:
2994 1917 1384 1128 373 268 172
AfeI AGC'GCT
Cuts at: 3095 3095
Size: 8236
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8039
AflIII A'CryG_T
Cuts at: 1203 7135 8112 1203
Size: 5932 977 1327
Fragments arranged by size:
5932 1327 977
AgeI A'CCGG_T
Cuts at: 7067 7067
Size: 8236
AhdI GACnn_n'nnGTC
Cuts at: 6965 6965
Size: 8236
AleI CACnn'nnGTG
Cuts at: 1166 2576 2918 3326 1166
Size: 1410 342 408 6076
Fragments arranged by size:
6076 1410 408 342
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2936 4077 4182 1184
Size: 1752 1141 105 5238
Fragments arranged by size:
5238 1752 1141 105
AlwNI CAG_nnn'CTG
Cuts at: 2047 2276 2512 2647 2734 2765 3724 3907 4621
Size: 229 236 135 87 31 959 183 714
Cuts at: 4621 4904 5979 7551 2047
Size: 283 1075 1572 2732
Fragments arranged by size:
2732 1572 1075 959 714 283 236 229
183 135 87 31
ApaI G_GGCC'C
Cuts at: 2524 3622 3718 4879 2524
Size: 1098 96 1161 5881
Fragments arranged by size:
5881 1161 1098 96
ApaLI G'TGCA_C
Cuts at: 3565 6293 7449 3565
Size: 2728 1156 4352
Fragments arranged by size:
4352 2728 1156
ApoI r'AATT_y
Cuts at: 1493 4589 5545 5771 5923 8032 1493
Size: 3096 956 226 152 2109 1697
Fragments arranged by size:
3096 2109 1697 956 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6790 160
Size: 872 5758 1606
Fragments arranged by size:
5758 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
AvaI C'yCGr_G
Cuts at: 1870 1943 2627 3621 3876 4092 4604 4809 4872
Size: 73 684 994 255 216 512 205 63
Cuts at: 4872 5408 5450 5561 1870
Size: 536 42 111 4545
Fragments arranged by size:
4545 994 684 536 512 255 216 205
111 73 63 42
BamHI G'GATC_C
Cuts at: 5566 5933 5566
Size: 367 7869
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2912 3686 4158 4608 4944
Size: 325 217 627 1125 774 472 450 336
Cuts at: 4944 5090 5404 5557 7012 618
Size: 146 314 153 1455 1842
Fragments arranged by size:
1842 1455 1125 774 627 472 450 336
325 314 217 153 146
BanII G_rGCy'C
Cuts at: 1391 2336 2524 3132 3622 3718 4651 4879 5555
Size: 945 188 608 490 96 933 228 676
Cuts at: 5555 1391
Size: 4072
Fragments arranged by size:
4072 945 933 676 608 490 228 188
96
BbeI G_GCGC'C
Cuts at: 5408 5408
Size: 8236
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2190 2867 3353 3568 4280 928
Size: 971 291 677 486 215 712 4884
Fragments arranged by size:
4884 971 712 677 486 291 215
BbvCI CC'TCA_GC
Cuts at: 5433 5433
Size: 8236
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3175 3209 6427 6461 1903
Size: 34 1238 34 3218 34 3678
Fragments arranged by size:
3678 3218 1238 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 6128 7338 6128
Size: 1210 7026
BclI T'GATC_A
Cuts at: 1548 1548
Size: 8236
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2144 2756 3923
Size: 107 122 71 631 605 472 612 1167
Cuts at: 3923 4334 4343 5298 5341 6847 136
Size: 411 9 955 43 1506 1525
Fragments arranged by size:
1525 1506 1167 955 631 612 605 472
411 122 107 71 43 9
BglII A'GATC_T
Cuts at: 4245 8231 4245
Size: 3986 4250
BlpI GC'TnA_GC
Cuts at: 4776 5653 4776
Size: 877 7359
Bme1580I G_kGCm'C
Cuts at: 1790 2270 2524 3152 3569 3622 3689 3718 3928
Size: 480 254 628 417 53 67 29 210
Cuts at: 3928 4161 4192 4330 4790 4879 5093 6297 7453
Size: 233 31 138 460 89 214 1204 1156
Cuts at: 7453 1790
Size: 2573
Fragments arranged by size:
2573 1204 1156 628 480 460 417 254
233 214 210 138 89 67 53 31
29
BmgBI CAC'GTC
Cuts at: 1318 3328 1318
Size: 2010 6226
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3473 3712 3728
Size: 416 53 83 399 24 2056 239 16
Cuts at: 3728 3938 4469 5967 6925 442
Size: 210 531 1498 958 1753
Fragments arranged by size:
2056 1753 1498 958 531 416 399 239
210 83 53 24 16
BmtI G_CTAG'C
Cuts at: 1056 4083 1056
Size: 3027 5209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2355 2355
Size: 8236
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3862 5345 6896 1239
Size: 2623 1483 1551 2579
Fragments arranged by size:
2623 2579 1551 1483
Bpu10I CC'TnA_GC
Cuts at: 2354 4319 5147 5433 2354
Size: 1965 828 286 5157
Fragments arranged by size:
5157 1965 828 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3116 3218 5141 6361 7226 7524 3116
Size: 102 1923 1220 865 298 3828
Fragments arranged by size:
3828 1923 1220 865 298 102
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2897 2911 4825 6899 882
Size: 617 1398 14 1914 2074 2219
Fragments arranged by size:
2219 2074 1914 1398 617 14
BsaAI yAC'GTr
Cuts at: 493 1204 6027 493
Size: 711 4823 2702
Fragments arranged by size:
4823 2702 711
BsaBI GATnn'nnATC
Cuts at: 8230 8230
Size: 8236
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2136 5405 6043 6425
Size: 53 83 186 936 603 3269 638 382
Cuts at: 6425 7060 275
Size: 635 1451
Fragments arranged by size:
3269 1451 936 638 635 603 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 6669 7067 7341 7488 7899 6669
Size: 398 274 147 411 7006
Fragments arranged by size:
7006 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2862 2892 3089 3119 4439
Size: 30 165 30 1459 30 197 30 1320
Cuts at: 4439 4469 4649 4679 5244 5274 5298 5328 1178
Size: 30 180 30 565 30 24 30 4086
Fragments arranged by size:
4086 1459 1320 565 197 180 165 30
30 30 30 30 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2326 3094 3965 4781 5252 1186
Size: 1140 768 871 816 471 4170
Fragments arranged by size:
4170 1140 871 816 768 471
BseYI C'CCAG_C
Cuts at: 2047 2249 2394 2542 2548 2591 2637 2724 3613
Size: 202 145 148 6 43 46 87 889
Cuts at: 3613 3632 3682 3711 3900 4548 4559 4882 5077
Size: 19 50 29 189 648 11 323 195
Cuts at: 5077 7439 7935 2047
Size: 2362 496 2348
Fragments arranged by size:
2362 2348 889 648 496 323 202 195
189 148 145 87 50 46 43 29
19 11 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2385 2942 3509 4106 4115 4230 4838
Size: 573 649 557 567 597 9 115 608
Cuts at: 4838 5021 5462 1163
Size: 183 441 3937
Fragments arranged by size:
3937 649 608 597 573 567 557 441
183 115 9
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3518 5269 5467 5686 6447 7475
Size: 275 186 1517 1751 198 219 761 1028
Cuts at: 7475 1540
Size: 2301
Fragments arranged by size:
2301 1751 1517 1028 761 275 219 198
186
BsiHKAI G_wGCw'C
Cuts at: 2336 3132 3333 3569 3768 4066 4434 4534 4803
Size: 796 201 236 199 298 368 100 269
Cuts at: 4803 5352 6297 6382 7453 2336
Size: 549 945 85 1071 3119
Fragments arranged by size:
3119 1071 945 796 549 368 298 269
236 201 199 100 85
BsmI GAATG_Cn'
Cuts at: 5747 5840 5747
Size: 93 8143
BsmBI CGTCTCn'nnnn_
Cuts at: 2695 3525 3775 4013 4089 2695
Size: 830 250 238 76 6842
Fragments arranged by size:
6842 830 250 238 76
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2270 2336 2524 3132 3152 3333 3569
Size: 399 480 66 188 608 20 181 236
Cuts at: 3569 3622 3689 3718 3768 3928 4066 4161 4192
Size: 53 67 29 50 160 138 95 31
Cuts at: 4192 4330 4434 4534 4651 4790 4803 4879 5093
Size: 138 104 100 117 139 13 76 214
Cuts at: 5093 5352 5555 6297 6382 7453 1391
Size: 259 203 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 608 480 399 259 236
214 203 188 181 160 139 138 138
117 104 100 95 85 76 67 66
53 50 31 29 20 13
BspEI T'CCGG_A
Cuts at: 7899 7899
Size: 8236
BspHI T'CATG_A
Cuts at: 6125 7855 6125
Size: 1730 6506
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2788 2879 4005 4620 4812 4956 5028 5391
Size: 1944 91 1126 615 192 144 72 363
Cuts at: 5391 5591 844
Size: 200 3489
Fragments arranged by size:
3489 1944 1126 615 363 200 192 144
91 72
BsrBI CCG'CTC
Cuts at: 1394 1823 2358 3102 3549 4780 6123 1394
Size: 429 535 744 447 1231 1343 3507
Fragments arranged by size:
3507 1343 1231 744 535 447 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 6731 6905 65
Size: 1183 5483 174 1396
Fragments arranged by size:
5483 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3012 3933 4694 6880 7067 1700
Size: 110 1202 921 761 2186 187 2869
Fragments arranged by size:
2869 2186 1202 921 761 187 110
BsrGI T'GTAC_A
Cuts at: 96 4455 96
Size: 4359 3877
BssHII G'CGCG_C
Cuts at: 1925 5441 1925
Size: 3516 4720
BssSI C'ACGA_G
Cuts at: 6296 7308 6296
Size: 1012 7224
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2647 4129 4208 4217 794
Size: 1853 1482 79 9 4813
Fragments arranged by size:
4813 1853 1482 79 9
BstBI TT'CG_AA
Cuts at: 2181 7040 2181
Size: 4859 3377
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8236
BstXI CCAn_nnnn'nTGG
Cuts at: 2131 3056 3668 3741 4177 2131
Size: 925 612 73 436 6190
Fragments arranged by size:
6190 925 612 436 73
BstYI r'GATC_y
Cuts at: 1972 2790 3136 3289 4245 5566 5933 6318 6335
Size: 818 346 153 956 1321 367 385 17
Cuts at: 6335 7787 7873 7885 8231 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
1977 1452 1321 956 818 385 367 346
346 153 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 5156 5156
Size: 8236
BtgI C'CryG_G
Cuts at: 513 1065 2004 2166 2754 3055 4137 4286 4392
Size: 552 939 162 588 301 1082 149 106
Cuts at: 4392 4442 5214 5330 513
Size: 50 772 116 3419
Fragments arranged by size:
3419 1082 939 772 588 552 301 162
149 116 106 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3833 487
Size: 683 56 2607 4890
Fragments arranged by size:
4890 2607 683 56
BtsI GCAGTG_nn'
Cuts at: 3258 3316 3442 3457 3463 4164 4330 4382 5752
Size: 58 126 15 6 701 166 52 1370
Cuts at: 5752 6546 6566 3258
Size: 794 20 4928
Fragments arranged by size:
4928 1370 794 701 166 126 58 52
20 15 6
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8236
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 4275 4310 529
Size: 35 3711 35 4455
Fragments arranged by size:
4455 3711 35 35
DraI TTT'AAA
Cuts at: 5540 5540
Size: 8236
DraIII CAC_nnn'GTG
Cuts at: 4352 4393 4352
Size: 41 8195
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3479 7243 809
Size: 507 2163 3764 1802
Fragments arranged by size:
3764 2163 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3515 4239 4464
Size: 54 1425 50 275 18 1685 724 225
Cuts at: 4464 4683 4710 5683 6572 8201 8
Size: 219 27 973 889 1629 43
Fragments arranged by size:
1685 1629 1425 973 889 724 275 225
219 54 50 43 27 18
EagI C'GGCC_G
Cuts at: 1537 1812 3515 5683 1537
Size: 275 1703 2168 4090
Fragments arranged by size:
4090 2168 1703 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2460 6166 1353
Size: 1107 3706 3423
Fragments arranged by size:
3706 3423 1107
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2970 5104 5333 6823 7197 7343
Size: 1064 138 1381 2134 229 1490 374 146
Cuts at: 7343 387
Size: 1280
Fragments arranged by size:
2134 1490 1381 1280 1064 374 229 146
138
EcoICRI GAG'CTC
Cuts at: 2334 3130 2334
Size: 796 7440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2005 2177 3305 3862 5345 6299 6896
Size: 393 373 172 1128 557 1483 954 597
Cuts at: 6896 7683 7951 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1483 1128 954 787 597 557 393
373 268 172
EcoNI CCTnn'n_nnAGG
Cuts at: 2580 2601 4052 4490 5207 2580
Size: 21 1451 438 717 5609
Fragments arranged by size:
5609 1451 717 438 21
EcoO109I rG'GnC_Cy
Cuts at: 2520 2521 2680 2800 2930 3034 3618 3824 4045
Size: 1 159 120 130 104 584 206 221
Cuts at: 4045 4876 5103 5143 5291 2520
Size: 831 227 40 148 5465
Fragments arranged by size:
5465 831 584 227 221 206 159 148
130 120 104 40 1
EcoRI G'AATT_C
Cuts at: 4589 5545 5923 4589
Size: 956 378 6902
Fragments arranged by size:
6902 956 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2207 2207
Size: 8236
FseI GG_CCGG'CC
Cuts at: 3937 3937
Size: 8236
FspI TGC'GCA
Cuts at: 3185 3257 5036 6742 3185
Size: 72 1779 1706 4679
Fragments arranged by size:
4679 1779 1706 72
FspAI rTGC'GCAy
Cuts at: 5036 5036
Size: 8236
HaeII r_GCGC'y
Cuts at: 1678 2048 2078 2375 3097 4086 4092 4184 5408
Size: 370 30 297 722 989 6 92 1224
Cuts at: 5408 7383 1678
Size: 1975 2531
Fragments arranged by size:
2531 1975 1224 989 722 370 297 92
30 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2279 2311 3281 3313 3377
Size: 32 135 32 707 32 970 32 64
Cuts at: 3377 3409 4208 4240 6877 6909 6951 6983 1373
Size: 32 799 32 2637 32 42 32 2626
Fragments arranged by size:
2637 2626 970 799 707 135 64 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 5580 5826 669
Size: 988 281 3642 246 3079
Fragments arranged by size:
3642 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 5456 748
Size: 4708 3528
HpaI GTT'AAC
Cuts at: 5826 5826
Size: 8236
KasI G'GCGC_C
Cuts at: 5404 5404
Size: 8236
KpnI G_GTAC'C
Cuts at: 4612 4948 5561 4612
Size: 336 613 7287
Fragments arranged by size:
7287 613 336
MfeI C'AATT_G
Cuts at: 5835 5835
Size: 8236
MluI A'CGCG_T
Cuts at: 8112 8112
Size: 8236
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5161 7350 7534 1305
Size: 3856 2189 184 2007
Fragments arranged by size:
3856 2189 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 4685 4712 10
Size: 54 1425 343 2853 27 3534
Fragments arranged by size:
3534 2853 1425 343 54 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2576 2918 3326 6194 6553 6712 518
Size: 648 1410 342 408 2868 359 159 2042
Fragments arranged by size:
2868 2042 1410 648 408 359 342 159
MspA1I CmG'CkG
Cuts at: 1606 2647 2747 3023 3444 3514 3907 4023 4129
Size: 1041 100 276 421 70 393 116 106
Cuts at: 4129 4574 4618 4629 4736 4792 5005 5332 5652
Size: 445 44 11 107 56 213 327 320
Cuts at: 5652 6329 7477 7722 1606
Size: 677 1148 245 2120
Fragments arranged by size:
2120 1148 1041 677 445 421 393 327
320 276 245 213 116 107 106 100
70 56 44 11
NaeI GCC'GGC
Cuts at: 3935 3935
Size: 8236
NarI GG'CG_CC
Cuts at: 5405 5405
Size: 8236
NcoI C'CATG_G
Cuts at: 513 1065 2004 2166 4137 4442 513
Size: 552 939 162 1971 305 4307
Fragments arranged by size:
4307 1971 939 552 305 162
NdeI CA'TA_TG
Cuts at: 387 387
Size: 8236
NgoMIV G'CCGG_C
Cuts at: 3933 3933
Size: 8236
NheI G'CTAG_C
Cuts at: 1052 4079 1052
Size: 3027 5209
NotI GC'GGCC_GC
Cuts at: 5683 5683
Size: 8236
NspI r_CATG'y
Cuts at: 1132 1229 2191 3541 5428 5594 7139 1132
Size: 97 962 1350 1887 166 1545 2229
Fragments arranged by size:
2229 1887 1545 1350 962 166 97
PasI CC'CwG_GG
Cuts at: 1348 2447 3668 4565 4842 5086 1348
Size: 1099 1221 897 277 244 4498
Fragments arranged by size:
4498 1221 1099 897 277 244
PciI A'CATG_T
Cuts at: 7135 7135
Size: 8236
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2716 4377 4393 4719 5046 1108
Size: 1608 1661 16 326 327 4298
Fragments arranged by size:
4298 1661 1608 327 326 16
PfoI T'CCnGG_A
Cuts at: 2599 4509 2599
Size: 1910 6326
PmeI GTTT'AAAC
Cuts at: 5540 5540
Size: 8236
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6305 6337 7818 7850 6305
Size: 32 1481 32 6691
Fragments arranged by size:
6691 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2680 2800 5143 2680
Size: 120 2343 5773
Fragments arranged by size:
5773 2343 120
PshAI GACnn'nnGTC
Cuts at: 1613 5271 5522 1613
Size: 3658 251 4327
Fragments arranged by size:
4327 3658 251
PsiI TTA'TAA
Cuts at: 5806 5806
Size: 8236
PspOMI G'GGCC_C
Cuts at: 2520 3618 3714 4875 2520
Size: 1098 96 1161 5881
Fragments arranged by size:
5881 1161 1098 96
PstI C_TGCA'G
Cuts at: 830 2493 2817 4005 4053 4617 4821 4953 5588
Size: 1663 324 1188 48 564 204 132 635
Cuts at: 5588 830
Size: 3478
Fragments arranged by size:
3478 1663 1188 635 564 324 204 132
48
PvuI CG_AT'CG
Cuts at: 2001 5467 2001
Size: 3466 4770
PvuII CAG'CTG
Cuts at: 2747 3023 3907 4129 4574 4618 4629 4736 5005
Size: 276 884 222 445 44 11 107 269
Cuts at: 5005 2747
Size: 5978
Fragments arranged by size:
5978 884 445 276 269 222 107 44
11
SacI G_AGCT'C
Cuts at: 2336 3132 2336
Size: 796 7440
SacII CC_GC'GG
Cuts at: 5333 5333
Size: 8236
SalI G'TCGA_C
Cuts at: 1936 5578 1936
Size: 3642 4594
SanDI GG'GwC_CC
Cuts at: 2680 2680
Size: 8236
SbfI CC_TGCA'GG
Cuts at: 4953 5588 4953
Size: 635 7601
ScaI AGT'ACT
Cuts at: 6484 8196 6484
Size: 1712 6524
SfcI C'TryA_G
Cuts at: 826 1045 2489 2613 2813 4001 4049 4613 4657
Size: 219 1444 124 200 1188 48 564 44
Cuts at: 4657 4817 4949 5584 6719 7400 7591 826
Size: 160 132 635 1135 681 191 1471
Fragments arranged by size:
1471 1444 1188 1135 681 635 564 219
200 191 160 132 124 48 44
SfoI GGC'GCC
Cuts at: 5406 5406
Size: 8236
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8236
SgrAI Cr'CCGG_yG
Cuts at: 1700 4694 1700
Size: 2994 5242
SmaI CCC'GGG
Cuts at: 1872 2629 3623 3878 4811 4874 5410 5563 1872
Size: 757 994 255 933 63 536 153 4545
Fragments arranged by size:
4545 994 933 757 536 255 153 63
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3131 3233 5120 5450 6340 7241
Size: 197 926 1188 102 1887 330 890 901
Cuts at: 7241 7503 820
Size: 262 1553
Fragments arranged by size:
1887 1553 1188 926 901 890 330 262
197 102
SnaBI TAC'GTA
Cuts at: 493 6027 493
Size: 5534 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8236
SphI G_CATG'C
Cuts at: 1132 3541 5428 5594 1132
Size: 2409 1887 166 3774
Fragments arranged by size:
3774 2409 1887 166
SrfI GCCC'GGGC
Cuts at: 4874 5410 4874
Size: 536 7700
SspI AAT'ATT
Cuts at: 5 52 6160 5
Size: 47 6108 2081
Fragments arranged by size:
6108 2081 47
StuI AGG'CCT
Cuts at: 1517 4337 5360 1517
Size: 2820 1023 4393
Fragments arranged by size:
4393 2820 1023
StyI C'CwwG_G
Cuts at: 513 1065 2004 2141 2166 3421 3600 3774 4137
Size: 552 939 137 25 1255 179 174 363
Cuts at: 4137 4442 4893 5675 513
Size: 305 451 782 3074
Fragments arranged by size:
3074 1255 939 782 552 451 363 305
179 174 137 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2099 2419 2651 2717 4003 6263 6280
Size: 1255 519 320 232 66 1286 2260 17
Cuts at: 6280 6433 6618 7063 325
Size: 153 185 445 1498
Fragments arranged by size:
2260 1498 1286 1255 519 445 320 232
185 153 66 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3834 4455 6482
Size: 274 80 33 51 1443 1857 621 2027
Cuts at: 6482 8194 96
Size: 1712 138
Fragments arranged by size:
2027 1857 1712 1443 621 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2469 2501 2523 2555 2862 2894 4299 4331 5160
Size: 32 22 32 307 32 1405 32 829
Cuts at: 5160 5192 5244 5276 5298 5330 8196 8228 2469
Size: 32 52 32 22 32 2866 32 2477
Fragments arranged by size:
2866 2477 1405 829 307 52 32 32
32 32 32 32 32 32 22 22
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 8236
XbaI T'CTAG_A
Cuts at: 5572 5572
Size: 8236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3952 3952
Size: 8236
XhoI C'TCGA_G
Cuts at: 1943 5450 1943
Size: 3507 4729
XmaI C'CCGG_G
Cuts at: 1870 2627 3621 3876 4809 4872 5408 5561 1870
Size: 757 994 255 933 63 536 153 4545
Fragments arranged by size:
4545 994 933 757 536 255 153 63
XmnI GAAnn'nnTTC
Cuts at: 6365 6365
Size: 8236
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 6044 276
Size: 53 83 186 936 4510 2468
Fragments arranged by size:
4510 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI
FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SacI SacII SalI SanDI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV
NruI NsiI PacI PmlI PspXI PsrI RsrII SapI
SexAI SfiI SwaI