Restriction map for
pFN21AB9891
(Circular) (Six-base) MAPSORT of: pFN21AB9891.seq Check: 9556 from: 1 to: 6106
pFN21AB9891 6106 bp
With 182 enzymes: SgfI *
October 20, 2009 10:35 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3916 278
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3449 1937
Size: 1512 4594
Acc65I G'GTAC_C
Cuts at: 3427 3427
Size: 6106
AclI AA'CG_TT
Cuts at: 1563 4233 4606 1563
Size: 2670 373 3063
Fragments arranged by size:
3063 2670 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3170 3366 4169 5553 5821 1632
Size: 1538 196 803 1384 268 1917
Fragments arranged by size:
1917 1538 1384 803 268 196
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5909
AflIII A'CryG_T
Cuts at: 1203 2377 5005 5982 1203
Size: 1174 2628 977 1327
Fragments arranged by size:
2628 1327 1174 977
AgeI A'CCGG_T
Cuts at: 4937 4937
Size: 6106
AhdI GACnn_n'nnGTC
Cuts at: 4835 4835
Size: 6106
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6106
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6106
AlwNI CAG_nnn'CTG
Cuts at: 2816 3849 5421 2816
Size: 1033 1572 3501
Fragments arranged by size:
3501 1572 1033
ApaLI G'TGCA_C
Cuts at: 3238 4163 5319 3238
Size: 925 1156 4025
Fragments arranged by size:
4025 1156 925
ApoI r'AATT_y
Cuts at: 1493 2253 2923 3129 3170 3415 3641 3793 5902
Size: 760 670 206 41 245 226 152 2109
Cuts at: 5902 1493
Size: 1697
Fragments arranged by size:
2109 1697 760 670 245 226 206 152
41
AseI AT'TA_AT
Cuts at: 160 1032 4660 160
Size: 872 3628 1606
Fragments arranged by size:
3628 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
AvaI C'yCGr_G
Cuts at: 1870 1943 3431 1870
Size: 73 1488 4545
Fragments arranged by size:
4545 1488 73
BamHI G'GATC_C
Cuts at: 3374 3436 3803 3374
Size: 62 367 5677
Fragments arranged by size:
5677 367 62
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3108 3427 4882 618
Size: 325 217 627 1321 319 1455 1842
Fragments arranged by size:
1842 1455 1321 627 325 319 217
BanII G_rGCy'C
Cuts at: 1391 3083 3425 1391
Size: 1692 342 4072
Fragments arranged by size:
4072 1692 342
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5135
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4297 4331 1903
Size: 34 2360 34 3678
Fragments arranged by size:
3678 2360 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2287 2365 3998 5208 2287
Size: 78 1633 1210 3185
Fragments arranged by size:
3185 1633 1210 78
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6106
BfrBI ATG'CAT
Cuts at: 2879 2879
Size: 6106
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4717 136
Size: 107 122 71 631 605 3045 1525
Fragments arranged by size:
3045 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 3153 6101 3153
Size: 2948 3158
BlpI GC'TnA_GC
Cuts at: 3523 3523
Size: 6106
Bme1580I G_kGCm'C
Cuts at: 1790 2547 3242 4167 5323 1790
Size: 757 695 925 1156 2573
Fragments arranged by size:
2573 1156 925 757 695
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6106
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3837 4795 442
Size: 416 53 83 399 24 2420 958 1753
Fragments arranged by size:
2420 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6106
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2100 2333 2517 2724 4766 1239
Size: 861 233 184 207 2042 2579
Fragments arranged by size:
2579 2042 861 233 207 184
Bpu10I CC'TnA_GC
Cuts at: 2941 2941
Size: 6106
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2207 2417 3379 4231 5096 5394 2207
Size: 210 962 852 865 298 2919
Fragments arranged by size:
2919 962 865 852 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2337 4769 882
Size: 617 838 2432 2219
Fragments arranged by size:
2432 2219 838 617
BsaAI yAC'GTr
Cuts at: 493 1204 3897 493
Size: 711 2693 2702
Fragments arranged by size:
2702 2693 711
BsaBI GATnn'nnATC
Cuts at: 6100 6100
Size: 6106
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3913 4295 4930 275
Size: 53 83 186 936 2380 382 635 1451
Fragments arranged by size:
2380 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4539 4937 5211 5358 5769 4539
Size: 398 274 147 411 4876
Fragments arranged by size:
4876 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2653 2683 2722 2752 3289
Size: 30 165 30 1250 30 39 30 537
Cuts at: 3289 3319 1178
Size: 30 3965
Fragments arranged by size:
3965 1250 537 165 39 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2097 2109 3021 1186
Size: 911 12 912 4271
Fragments arranged by size:
4271 912 911 12
BseYI C'CCAG_C
Cuts at: 2050 2623 5309 5805 2050
Size: 573 2686 496 2351
Fragments arranged by size:
2686 2351 573 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3095 1163
Size: 573 1359 4174
Fragments arranged by size:
4174 1359 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3556 4317 5345 1540
Size: 275 186 1555 761 1028 2301
Fragments arranged by size:
2301 1555 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3242 4167 4252 5323 3242
Size: 925 85 1071 4025
Fragments arranged by size:
4025 1071 925 85
BsmI GAATG_Cn'
Cuts at: 3617 3710 3617
Size: 93 6013
BsmBI CGTCTCn'nnnn_
Cuts at: 2351 2351
Size: 6106
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2547 3083 3242 3425 4167 4252 5323
Size: 399 757 536 159 183 742 85 1071
Cuts at: 5323 1391
Size: 2174
Fragments arranged by size:
2174 1071 757 742 536 399 183 159
85
BspEI T'CCGG_A
Cuts at: 5769 5769
Size: 6106
BspHI T'CATG_A
Cuts at: 2425 3995 5725 2425
Size: 1570 1730 2806
Fragments arranged by size:
2806 1730 1570
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2824 3461 844
Size: 1980 637 3489
Fragments arranged by size:
3489 1980 637
BsrBI CCG'CTC
Cuts at: 1394 1823 3993 1394
Size: 429 2170 3507
Fragments arranged by size:
3507 2170 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4601 4775 65
Size: 1183 3353 174 1396
Fragments arranged by size:
3353 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4750 4937 1700
Size: 110 2940 187 2869
Fragments arranged by size:
2940 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6106
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6106
BssSI C'ACGA_G
Cuts at: 4166 5178 4166
Size: 1012 5094
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6106
BstBI TT'CG_AA
Cuts at: 4910 4910
Size: 6106
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6106
BstYI r'GATC_y
Cuts at: 1972 2559 3153 3374 3436 3803 4188 4205 5657
Size: 587 594 221 62 367 385 17 1452
Cuts at: 5657 5743 5755 6101 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 594 587 385 367 346 221
86 62 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2973 513
Size: 552 1908 3646
Fragments arranged by size:
3646 1908 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5367
Fragments arranged by size:
5367 683 56
BtsI GCAGTG_nn'
Cuts at: 2142 3622 4416 4436 2142
Size: 1480 794 20 3812
Fragments arranged by size:
3812 1480 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6106
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6071
DraI TTT'AAA
Cuts at: 3410 3410
Size: 6106
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5113 809
Size: 507 3797 1802
Fragments arranged by size:
3797 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3553 4442 6071
Size: 54 1425 50 275 18 1723 889 1629
Cuts at: 6071 8
Size: 43
Fragments arranged by size:
1723 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3553 1537
Size: 275 1741 4090
Fragments arranged by size:
4090 1741 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2036 2998 3186 4036 1353
Size: 683 962 188 850 3423
Fragments arranged by size:
3423 962 850 683 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2106 2565 4693 5067 5213 387
Size: 1064 138 517 459 2128 374 146 1280
Fragments arranged by size:
2128 1280 1064 517 459 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2100 2333 2517 2724 3170 3366 4169
Size: 393 468 233 184 207 446 196 803
Cuts at: 4169 4766 5553 5821 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 803 787 597 468 446 393 268
233 207 196 184
EcoRI G'AATT_C
Cuts at: 3415 3793 3415
Size: 378 5728
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3324 3324
Size: 6106
FspI TGC'GCA
Cuts at: 4612 4612
Size: 6106
HaeII r_GCGC'y
Cuts at: 1678 2404 5253 1678
Size: 726 2849 2531
Fragments arranged by size:
2849 2531 726
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2720 2752 4747 4779 4821
Size: 32 135 32 1148 32 1995 32 42
Cuts at: 4821 4853 1373
Size: 32 2626
Fragments arranged by size:
2626 1995 1148 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2962 3450 3696 669
Size: 988 281 1024 488 246 3079
Fragments arranged by size:
3079 1024 988 488 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6106
HpaI GTT'AAC
Cuts at: 2962 3696 2962
Size: 734 5372
KpnI G_GTAC'C
Cuts at: 3431 3431
Size: 6106
MfeI C'AATT_G
Cuts at: 2439 3085 3705 2439
Size: 646 620 4840
Fragments arranged by size:
4840 646 620
MluI A'CGCG_T
Cuts at: 5982 5982
Size: 6106
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2842 3330 5220 5404 1305
Size: 1537 488 1890 184 2007
Fragments arranged by size:
2007 1890 1537 488 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4284
Fragments arranged by size:
4284 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2824 3248 3330 4064 4423 4582 518
Size: 648 1658 424 82 734 359 159 2042
Fragments arranged by size:
2042 1658 734 648 424 359 159 82
MspA1I CmG'CkG
Cuts at: 1606 2069 3522 4199 5347 5592 1606
Size: 463 1453 677 1148 245 2120
Fragments arranged by size:
2120 1453 1148 677 463 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5554
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6106
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6106
NotI GC'GGCC_GC
Cuts at: 3553 3553
Size: 6106
NsiI A_TGCA'T
Cuts at: 2881 2881
Size: 6106
NspI r_CATG'y
Cuts at: 1132 1229 2381 3403 3464 5009 1132
Size: 97 1152 1022 61 1545 2229
Fragments arranged by size:
2229 1545 1152 1022 97 61
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6106
PciI A'CATG_T
Cuts at: 2377 5005 2377
Size: 2628 3478
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2672 1108
Size: 1564 4542
PfoI T'CCnGG_A
Cuts at: 2671 2671
Size: 6106
PmeI GTTT'AAAC
Cuts at: 3410 3410
Size: 6106
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4175 4207 5688 5720 4175
Size: 32 1481 32 4561
Fragments arranged by size:
4561 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6106
PsiI TTA'TAA
Cuts at: 3676 3676
Size: 6106
PstI C_TGCA'G
Cuts at: 830 3458 830
Size: 2628 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6106
PvuII CAG'CTG
Cuts at: 2069 2069
Size: 6106
SalI G'TCGA_C
Cuts at: 1936 3448 1936
Size: 1512 4594
SapI GCTCTTCn'nnn_
Cuts at: 3186 3186
Size: 6106
SbfI CC_TGCA'GG
Cuts at: 3458 3458
Size: 6106
ScaI AGT'ACT
Cuts at: 3304 4354 6066 3304
Size: 1050 1712 3344
Fragments arranged by size:
3344 1712 1050
SexAI A'CCwGG_T
Cuts at: 2327 2327
Size: 6106
SfcI C'TryA_G
Cuts at: 826 1045 2898 3341 3454 4589 5270 5461 826
Size: 219 1853 443 113 1135 681 191 1471
Fragments arranged by size:
1853 1471 1135 681 443 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6106
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6106
SmaI CCC'GGG
Cuts at: 1872 3433 1872
Size: 1561 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2186 2432 3394 4210 5111 5373
Size: 197 926 243 246 962 816 901 262
Cuts at: 5373 820
Size: 1553
Fragments arranged by size:
1553 962 926 901 816 262 246 243
197
SnaBI TAC'GTA
Cuts at: 493 3897 493
Size: 3404 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6106
SphI G_CATG'C
Cuts at: 1132 3464 1132
Size: 2332 3774
SspI AAT'ATT
Cuts at: 5 52 4030 5
Size: 47 3978 2081
Fragments arranged by size:
3978 2081 47
StuI AGG'CCT
Cuts at: 1517 2304 1517
Size: 787 5319
StyI C'CwwG_G
Cuts at: 513 1065 3545 513
Size: 552 2480 3074
Fragments arranged by size:
3074 2480 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4133 4150 4303 4488 4933 325
Size: 1255 2553 17 153 185 445 1498
Fragments arranged by size:
2553 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2267 3302 4352
Size: 274 80 33 51 1443 290 1035 1050
Cuts at: 4352 6064 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1050 1035 290 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2105 2137 2722 2754 2994 3026 6066 6098 2105
Size: 32 585 32 240 32 3040 32 2113
Fragments arranged by size:
3040 2113 585 240 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6106
XbaI T'CTAG_A
Cuts at: 3442 3442
Size: 6106
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6106
XmaI C'CCGG_G
Cuts at: 1870 3431 1870
Size: 1561 4545
XmnI GAAnn'nnTTC
Cuts at: 3155 4235 3155
Size: 1080 5026
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3914 276
Size: 53 83 186 936 2380 2468
Fragments arranged by size:
2468 2380 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI
PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI
EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI
NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI PsrI
RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI
XcmI