Restriction map for
pFN21AB9885
(Circular) (Six-base) MAPSORT of: pFN21AB9885.seq Check: 1582 from: 1 to: 6664
pFN21AB9885 6664 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3031 3031
Size: 6664
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4474 278
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3187 3585 4007 1937
Size: 1250 398 422 4594
Fragments arranged by size:
4594 1250 422 398
Acc65I G'GTAC_C
Cuts at: 3985 3985
Size: 6664
AclI AA'CG_TT
Cuts at: 1563 4791 5164 1563
Size: 3228 373 3063
Fragments arranged by size:
3228 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2519 2658 4727 6111 6379 1632
Size: 887 139 2069 1384 268 1917
Fragments arranged by size:
2069 1917 1384 887 268 139
AflII C'TTAA_G
Cuts at: 820 1017 2846 820
Size: 197 1829 4638
Fragments arranged by size:
4638 1829 197
AflIII A'CryG_T
Cuts at: 1203 5563 6540 1203
Size: 4360 977 1327
Fragments arranged by size:
4360 1327 977
AgeI A'CCGG_T
Cuts at: 5495 5495
Size: 6664
AhdI GACnn_n'nnGTC
Cuts at: 5393 5393
Size: 6664
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6664
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 3331 1184
Size: 2147 4517
AlwNI CAG_nnn'CTG
Cuts at: 2146 2945 3325 3895 4407 5979 2146
Size: 799 380 570 512 1572 2831
Fragments arranged by size:
2831 1572 799 570 512 380
ApaLI G'TGCA_C
Cuts at: 4721 5877 4721
Size: 1156 5508
ApoI r'AATT_y
Cuts at: 1493 3554 3798 3973 4199 4351 6460 1493
Size: 2061 244 175 226 152 2109 1697
Fragments arranged by size:
2109 2061 1697 244 226 175 152
AseI AT'TA_AT
Cuts at: 160 1032 5218 160
Size: 872 4186 1606
Fragments arranged by size:
4186 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
AvaI C'yCGr_G
Cuts at: 1870 1943 2814 3989 1870
Size: 73 871 1175 4545
Fragments arranged by size:
4545 1175 871 73
AvrII C'CTAG_G
Cuts at: 3011 3011
Size: 6664
BamHI G'GATC_C
Cuts at: 3994 4361 3994
Size: 367 6297
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3578 3985 5440 618
Size: 325 217 627 1791 407 1455 1842
Fragments arranged by size:
1842 1791 1455 627 407 325 217
BanII G_rGCy'C
Cuts at: 1391 2277 2370 2799 2823 2844 2990 3983 1391
Size: 886 93 429 24 21 146 993 4072
Fragments arranged by size:
4072 993 886 429 146 93 24 21
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5693
BbvCI CC'TCA_GC
Cuts at: 2430 3573 2430
Size: 1143 5521
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3671 3705 4855 4889 1903
Size: 34 1734 34 1150 34 3678
Fragments arranged by size:
3678 1734 1150 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4556 5766 4556
Size: 1210 5454
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6664
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2933 3240 5275
Size: 107 122 71 631 605 1261 307 2035
Cuts at: 5275 136
Size: 1525
Fragments arranged by size:
2035 1525 1261 631 605 307 122 107
71
BglII A'GATC_T
Cuts at: 3277 3927 6659 3277
Size: 650 2732 3282
Fragments arranged by size:
3282 2732 650
BlpI GC'TnA_GC
Cuts at: 2276 3260 3410 4081 2276
Size: 984 150 671 4859
Fragments arranged by size:
4859 984 671 150
Bme1580I G_kGCm'C
Cuts at: 1790 3581 4725 5881 1790
Size: 1791 1144 1156 2573
Fragments arranged by size:
2573 1791 1156 1144
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6664
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3231 3956 4395
Size: 416 53 83 399 24 1814 725 439
Cuts at: 4395 5353 442
Size: 958 1753
Fragments arranged by size:
1814 1753 958 725 439 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6664
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3067 3067
Size: 6664
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2168 2543 2786 3090 3635 3662 5324 1239
Size: 929 375 243 304 545 27 1662 2579
Fragments arranged by size:
2579 1662 929 545 375 304 243 27
Bpu10I CC'TnA_GC
Cuts at: 2430 3573 3601 2430
Size: 1143 28 5493
Fragments arranged by size:
5493 1143 28
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3062 3344 4789 5654 5952 3062
Size: 282 1445 865 298 3774
Fragments arranged by size:
3774 1445 865 298 282
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5327 882
Size: 617 3828 2219
Fragments arranged by size:
3828 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3139 4455 493
Size: 711 1935 1316 2702
Fragments arranged by size:
2702 1935 1316 711
BsaBI GATnn'nnATC
Cuts at: 6658 6658
Size: 6664
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2320 2440 4471 4853
Size: 53 83 186 936 787 120 2031 382
Cuts at: 4853 5488 275
Size: 635 1451
Fragments arranged by size:
2031 1451 936 787 635 382 186 120
83 53
BsaWI w'CCGG_w
Cuts at: 3859 5097 5495 5769 5916 6327 3859
Size: 1238 398 274 147 411 4196
Fragments arranged by size:
4196 1238 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2335 2365 1178
Size: 30 165 30 932 30 5477
Fragments arranged by size:
5477 932 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 2591 2684 2783 2798 2801 3285 1186
Size: 1261 144 93 99 15 3 484 4565
Fragments arranged by size:
4565 1261 484 144 99 93 15 3
BseYI C'CCAG_C
Cuts at: 2226 2270 3685 5867 6363 2226
Size: 44 1415 2182 496 2527
Fragments arranged by size:
2527 2182 1415 496 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2099 2723 1163
Size: 573 363 624 5104
Fragments arranged by size:
5104 624 573 363
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4114 4875 5903 1540
Size: 275 186 2113 761 1028 2301
Fragments arranged by size:
2301 2113 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2799 2823 2844 3636 4725 4810 5881 2799
Size: 24 21 792 1089 85 1071 3582
Fragments arranged by size:
3582 1089 1071 792 85 24 21
BsmI GAATG_Cn'
Cuts at: 2066 4175 4268 2066
Size: 2109 93 4462
Fragments arranged by size:
4462 2109 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2277 2370 2799 2823 2844 2990 3581
Size: 399 487 93 429 24 21 146 591
Cuts at: 3581 3636 3983 4725 4810 5881 1391
Size: 55 347 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 591 487 429 399 347
146 93 85 55 24 21
BspEI T'CCGG_A
Cuts at: 6327 6327
Size: 6664
BspHI T'CATG_A
Cuts at: 4553 6283 4553
Size: 1730 4934
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2691 3031 4019 844
Size: 1847 340 988 3489
Fragments arranged by size:
3489 1847 988 340
BsrBI CCG'CTC
Cuts at: 1394 1823 2437 4551 1394
Size: 429 614 2114 3507
Fragments arranged by size:
3507 2114 614 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2691 3315 3735 3916 5159 5333 65
Size: 1183 1443 624 420 181 1243 174 1396
Fragments arranged by size:
1443 1396 1243 1183 624 420 181 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 5308 5495 1700
Size: 110 3498 187 2869
Fragments arranged by size:
3498 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2405 96
Size: 2309 4355
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6664
BssSI C'ACGA_G
Cuts at: 3759 4724 5736 3759
Size: 965 1012 4687
Fragments arranged by size:
4687 1012 965
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6664
BstBI TT'CG_AA
Cuts at: 5468 5468
Size: 6664
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6664
BstXI CCAn_nnnn'nTGG
Cuts at: 2074 2567 2074
Size: 493 6171
BstYI r'GATC_y
Cuts at: 1972 3277 3927 3994 4361 4746 4763 6215 6301
Size: 1305 650 67 367 385 17 1452 86
Cuts at: 6301 6313 6659 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1305 650 385 367 346 86
67 17 12
BstZ17I GTA'TAC
Cuts at: 3188 3188
Size: 6664
Bsu36I CC'TnA_GG
Cuts at: 3042 3813 3882 3042
Size: 771 69 5824
Fragments arranged by size:
5824 771 69
BtgI C'CryG_G
Cuts at: 513 1065 2004 2928 3172 3419 513
Size: 552 939 924 244 247 3758
Fragments arranged by size:
3758 939 924 552 247 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5925
Fragments arranged by size:
5925 683 56
BtsI GCAGTG_nn'
Cuts at: 2799 3071 3382 4180 4974 4994 2799
Size: 272 311 798 794 20 4469
Fragments arranged by size:
4469 798 794 311 272 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6664
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6629
DraI TTT'AAA
Cuts at: 3968 3968
Size: 6664
DraIII CAC_nnn'GTG
Cuts at: 3388 3388
Size: 6664
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 3855 5671 809
Size: 507 2539 1816 1802
Fragments arranged by size:
2539 1816 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2122 2160 2398
Size: 54 1425 50 275 18 292 38 238
Cuts at: 2398 2733 3379 3445 4111 5000 6629 8
Size: 335 646 66 666 889 1629 43
Fragments arranged by size:
1629 1425 889 666 646 335 292 275
238 66 54 50 43 38 18
EagI C'GGCC_G
Cuts at: 1537 1812 4111 1537
Size: 275 2299 4090
Fragments arranged by size:
4090 2299 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2085 4594 1353
Size: 732 2509 3423
Fragments arranged by size:
3423 2509 732
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2515 3215 5251 5625 5771 387
Size: 1064 138 926 700 2036 374 146 1280
Fragments arranged by size:
2036 1280 1064 926 700 374 146 138
EcoICRI GAG'CTC
Cuts at: 2797 2821 2842 2797
Size: 24 21 6619
Fragments arranged by size:
6619 24 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2168 2519 2543 2658 2786 3090 3635
Size: 393 536 351 24 115 128 304 545
Cuts at: 3635 3662 4727 5324 6111 6379 1239
Size: 27 1065 597 787 268 1524
Fragments arranged by size:
1524 1065 787 597 545 536 393 351
304 268 128 115 27 24
EcoNI CCTnn'n_nnAGG
Cuts at: 3397 3397
Size: 6664
EcoO109I rG'GnC_Cy
Cuts at: 2530 3015 3038 3671 2530
Size: 485 23 633 5523
Fragments arranged by size:
5523 633 485 23
EcoRI G'AATT_C
Cuts at: 3973 4351 3973
Size: 378 6286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 6664
FspI TGC'GCA
Cuts at: 5170 5170
Size: 6664
HaeII r_GCGC'y
Cuts at: 1678 5811 1678
Size: 4133 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2224 2256 3491 3523 5305
Size: 32 135 32 652 32 1235 32 1782
Cuts at: 5305 5337 5379 5411 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1782 1235 652 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2463 2908 4008 4254 669
Size: 988 281 525 445 1100 246 3079
Fragments arranged by size:
3079 1100 988 525 445 281 246
HindIII A'AGCT_T
Cuts at: 748 2108 3262 3479 748
Size: 1360 1154 217 3933
Fragments arranged by size:
3933 1360 1154 217
HpaI GTT'AAC
Cuts at: 2463 2908 4254 2463
Size: 445 1346 4873
Fragments arranged by size:
4873 1346 445
KpnI G_GTAC'C
Cuts at: 3989 3989
Size: 6664
MfeI C'AATT_G
Cuts at: 4263 4263
Size: 6664
MluI A'CGCG_T
Cuts at: 6540 6540
Size: 6664
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5778 5962 1305
Size: 4473 184 2007
Fragments arranged by size:
4473 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2124 2162 2400 2735 3381
Size: 54 1425 343 292 38 238 335 646
Cuts at: 3381 3447 10
Size: 66 3227
Fragments arranged by size:
3227 1425 646 343 335 292 238 66
54 38
MslI CAynn'nnrTG
Cuts at: 518 1166 2072 3749 4622 4981 5140 518
Size: 648 906 1677 873 359 159 2042
Fragments arranged by size:
2042 1677 906 873 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2098 2184 2230 2270 2429 2497 2777 3034
Size: 492 86 46 40 159 68 280 257
Cuts at: 3034 3409 3528 3685 3732 3892 4080 4757 5905
Size: 375 119 157 47 160 188 677 1148
Cuts at: 5905 6150 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 492 375 280 257 245
188 160 159 157 119 86 68 47
46 40
NcoI C'CATG_G
Cuts at: 513 1065 2004 2928 3419 513
Size: 552 939 924 491 3758
Fragments arranged by size:
3758 939 924 552 491
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6664
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6664
NotI GC'GGCC_GC
Cuts at: 4111 4111
Size: 6664
NspI r_CATG'y
Cuts at: 1132 1229 3748 4022 5567 1132
Size: 97 2519 274 1545 2229
Fragments arranged by size:
2519 2229 1545 274 97
PasI CC'CwG_GG
Cuts at: 1348 3595 1348
Size: 2247 4417
PciI A'CATG_T
Cuts at: 5563 5563
Size: 6664
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2146 3388 1108
Size: 1038 1242 4384
Fragments arranged by size:
4384 1242 1038
PfoI T'CCnGG_A
Cuts at: 2046 2559 2589 3106 2046
Size: 513 30 517 5604
Fragments arranged by size:
5604 517 513 30
PmeI GTTT'AAAC
Cuts at: 3968 3968
Size: 6664
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4733 4765 6246 6278 4733
Size: 32 1481 32 5119
Fragments arranged by size:
5119 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2530 3038 2530
Size: 508 6156
PshAI GACnn'nnGTC
Cuts at: 1613 3786 1613
Size: 2173 4491
PsiI TTA'TAA
Cuts at: 4234 4234
Size: 6664
PstI C_TGCA'G
Cuts at: 830 2014 2496 4016 830
Size: 1184 482 1520 3478
Fragments arranged by size:
3478 1520 1184 482
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6664
PvuII CAG'CTG
Cuts at: 2098 2230 2270 2429 2777 3409 3528 3685 3892
Size: 132 40 159 348 632 119 157 207
Cuts at: 3892 2098
Size: 4870
Fragments arranged by size:
4870 632 348 207 159 157 132 119
40
SacI G_AGCT'C
Cuts at: 2799 2823 2844 2799
Size: 24 21 6619
Fragments arranged by size:
6619 24 21
SalI G'TCGA_C
Cuts at: 1936 4006 1936
Size: 2070 4594
SapI GCTCTTCn'nnn_
Cuts at: 2085 2085
Size: 6664
SbfI CC_TGCA'GG
Cuts at: 4016 4016
Size: 6664
ScaI AGT'ACT
Cuts at: 3159 4912 6624 3159
Size: 1753 1712 3199
Fragments arranged by size:
3199 1753 1712
SfcI C'TryA_G
Cuts at: 826 1045 2010 2492 3666 3826 3931 4012 5147
Size: 219 965 482 1174 160 105 81 1135
Cuts at: 5147 5828 6019 826
Size: 681 191 1471
Fragments arranged by size:
1471 1174 1135 965 681 482 219 191
160 105 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 2933 2933
Size: 6664
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6664
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6664
SmaI CCC'GGG
Cuts at: 1872 3991 1872
Size: 2119 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2846 3077 3359 4768 5669 5931
Size: 197 926 903 231 282 1409 901 262
Cuts at: 5931 820
Size: 1553
Fragments arranged by size:
1553 1409 926 903 901 282 262 231
197
SnaBI TAC'GTA
Cuts at: 493 4455 493
Size: 3962 2702
SpeI A'CTAG_T
Cuts at: 152 3160 152
Size: 3008 3656
SphI G_CATG'C
Cuts at: 1132 4022 1132
Size: 2890 3774
SspI AAT'ATT
Cuts at: 5 52 4588 5
Size: 47 4536 2081
Fragments arranged by size:
4536 2081 47
StuI AGG'CCT
Cuts at: 1517 3606 1517
Size: 2089 4575
StyI C'CwwG_G
Cuts at: 513 1065 2004 2511 2526 2928 3011 3268 3372
Size: 552 939 507 15 402 83 257 104
Cuts at: 3372 3419 4103 513
Size: 47 684 3074
Fragments arranged by size:
3074 939 684 552 507 402 257 104
83 47 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2295 3608 4691 4708 4861 5046 5491
Size: 1255 715 1313 1083 17 153 185 445
Cuts at: 5491 325
Size: 1498
Fragments arranged by size:
1498 1313 1255 1083 715 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2405 3157 4910
Size: 274 80 33 51 1443 428 752 1753
Cuts at: 4910 6622 96
Size: 1712 138
Fragments arranged by size:
1753 1712 1443 752 428 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3498 3530 6624 6656 3498
Size: 32 3094 32 3506
Fragments arranged by size:
3506 3094 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6664
XbaI T'CTAG_A
Cuts at: 4000 4000
Size: 6664
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 2659 3326 3644 2393
Size: 266 667 318 5413
Fragments arranged by size:
5413 667 318 266
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6664
XmaI C'CCGG_G
Cuts at: 1870 3989 1870
Size: 2119 4545
XmnI GAAnn'nnTTC
Cuts at: 2618 3952 4793 2618
Size: 1334 841 4489
Fragments arranged by size:
4489 1334 841
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4472 276
Size: 53 83 186 936 2938 2468
Fragments arranged by size:
2938 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PstI PvuI PvuII SacI SalI SapI SbfI ScaI
SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI
BsmBI EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII
SacII SanDI SexAI SfoI SrfI SwaI