Restriction map for
pFN21AB9881
(Circular) (Six-base) MAPSORT of: pFN21AB9881.seq Check: 6696 from: 1 to: 6502
pFN21AB9881 6502 bp
With 182 enzymes: SgfI *
October 20, 2009 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2204 2989 2204
Size: 785 5717
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4312 278
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3845 1937
Size: 1908 4594
Acc65I G'GTAC_C
Cuts at: 3672 3823 3672
Size: 151 6351
AclI AA'CG_TT
Cuts at: 1563 4629 5002 1563
Size: 3066 373 3063
Fragments arranged by size:
3066 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2162 4565 5949 6217 1632
Size: 530 2403 1384 268 1917
Fragments arranged by size:
2403 1917 1384 530 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6305
AflIII A'CryG_T
Cuts at: 1203 2574 5401 6378 1203
Size: 1371 2827 977 1327
Fragments arranged by size:
2827 1371 1327 977
AgeI A'CCGG_T
Cuts at: 5333 5333
Size: 6502
AhdI GACnn_n'nnGTC
Cuts at: 5231 5231
Size: 6502
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6502
AlwNI CAG_nnn'CTG
Cuts at: 2677 2824 4245 5817 2677
Size: 147 1421 1572 3362
Fragments arranged by size:
3362 1572 1421 147
ApaI G_GGCC'C
Cuts at: 2012 3436 2012
Size: 1424 5078
ApaLI G'TGCA_C
Cuts at: 4559 5715 4559
Size: 1156 5346
ApoI r'AATT_y
Cuts at: 1493 3811 4037 4189 6298 1493
Size: 2318 226 152 2109 1697
Fragments arranged by size:
2318 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5056 160
Size: 872 4024 1606
Fragments arranged by size:
4024 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
AvaI C'yCGr_G
Cuts at: 1870 1943 2234 2526 2923 3827 1870
Size: 73 291 292 397 904 4545
Fragments arranged by size:
4545 904 397 292 291 73
BamHI G'GATC_C
Cuts at: 3061 3832 4199 3061
Size: 771 367 5364
Fragments arranged by size:
5364 771 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2388 2461 2482 2963 3476
Size: 325 217 627 601 73 21 481 513
Cuts at: 3476 3619 3672 3823 5278 618
Size: 143 53 151 1455 1842
Fragments arranged by size:
1842 1455 627 601 513 481 325 217
151 143 73 53 21
BanII G_rGCy'C
Cuts at: 1391 2012 3404 3436 3821 1391
Size: 621 1392 32 385 4072
Fragments arranged by size:
4072 1392 621 385 32
BbeI G_GCGC'C
Cuts at: 2392 2465 2392
Size: 73 6429
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3591 928
Size: 971 1692 3839
Fragments arranged by size:
3839 1692 971
BbvCI CC'TCA_GC
Cuts at: 3423 3423
Size: 6502
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4693 4727 1903
Size: 34 2756 34 3678
Fragments arranged by size:
3678 2756 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4394 5604 4394
Size: 1210 5292
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6502
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2162 2474 2769
Size: 107 122 71 631 605 490 312 295
Cuts at: 2769 3122 3770 5113 136
Size: 353 648 1343 1525
Fragments arranged by size:
1525 1343 648 631 605 490 353 312
295 122 107 71
BglII A'GATC_T
Cuts at: 6497 6497
Size: 6502
BlpI GC'TnA_GC
Cuts at: 2129 3919 2129
Size: 1790 4712
Bme1580I G_kGCm'C
Cuts at: 1790 2012 2487 3436 3481 3500 4563 5719 1790
Size: 222 475 949 45 19 1063 1156 2573
Fragments arranged by size:
2573 1156 1063 949 475 222 45 19
BmgBI CAC'GTC
Cuts at: 1318 2575 1318
Size: 1257 5245
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2902 3439 3487
Size: 416 53 83 399 24 1485 537 48
Cuts at: 3487 4233 5191 442
Size: 746 958 1753
Fragments arranged by size:
1753 1485 958 746 537 416 399 83
53 48 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6502
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3418 3421 3418
Size: 3 6499
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2999 5162 1239
Size: 1760 2163 2579
Fragments arranged by size:
2579 2163 1760
Bpu10I CC'TnA_GC
Cuts at: 2678 3423 2678
Size: 745 5757
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2288 3017 3191 4627 5492 5790 2288
Size: 729 174 1436 865 298 3000
Fragments arranged by size:
3000 1436 865 729 298 174
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2585 3089 5165 882
Size: 617 1086 504 2076 2219
Fragments arranged by size:
2219 2076 1086 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 4293 493
Size: 711 3089 2702
Fragments arranged by size:
3089 2702 711
BsaBI GATnn'nnATC
Cuts at: 6496 6496
Size: 6502
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2165 2389 2462 4309
Size: 53 83 186 936 632 224 73 1847
Cuts at: 4309 4691 5326 275
Size: 382 635 1451
Fragments arranged by size:
1847 1451 936 635 632 382 224 186
83 73 53
BsaWI w'CCGG_w
Cuts at: 2074 4935 5333 5607 5754 6165 2074
Size: 2861 398 274 147 411 2411
Fragments arranged by size:
2861 2411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2162 2192 3665 3695 1178
Size: 30 165 30 759 30 1473 30 3985
Fragments arranged by size:
3985 1473 759 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2081 2345 2546 3242 3245 3248 3251 3263
Size: 895 264 201 696 3 3 3 12
Cuts at: 3263 3266 3293 3296 3308 3311 3314 3317 3356
Size: 3 27 3 12 3 3 3 39
Cuts at: 3356 3365 3368 3394 3398 3410 3617 1186
Size: 9 3 26 4 12 207 4071
Fragments arranged by size:
4071 895 696 264 207 201 39 27
26 12 12 12 9 4 3 3
3 3 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 2335 2670 3079 3629 5705 6201 2335
Size: 335 409 550 2076 496 2636
Fragments arranged by size:
2636 2076 550 496 409 335
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2960 3083 3725 1163
Size: 573 1224 123 642 3940
Fragments arranged by size:
3940 1224 642 573 123
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2478 2657 2896 3952 4713 5741
Size: 275 186 477 179 239 1056 761 1028
Cuts at: 5741 1540
Size: 2301
Fragments arranged by size:
2301 1056 1028 761 477 275 239 186
179
BsiHKAI G_wGCw'C
Cuts at: 2135 2510 3404 4563 4648 5719 2135
Size: 375 894 1159 85 1071 2918
Fragments arranged by size:
2918 1159 1071 894 375 85
BsmI GAATG_Cn'
Cuts at: 4013 4106 4013
Size: 93 6409
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2012 2135 2487 2510 3404 3436 3481
Size: 399 222 123 352 23 894 32 45
Cuts at: 3481 3500 3821 4563 4648 5719 1391
Size: 19 321 742 85 1071 2174
Fragments arranged by size:
2174 1071 894 742 399 352 321 222
123 85 45 32 23 19
BspEI T'CCGG_A
Cuts at: 2074 6165 2074
Size: 4091 2411
BspHI T'CATG_A
Cuts at: 4391 6121 4391
Size: 1730 4772
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2088 2204 2989 3857 844
Size: 1244 116 785 868 3489
Fragments arranged by size:
3489 1244 868 785 116
BsrBI CCG'CTC
Cuts at: 1394 1823 4389 1394
Size: 429 2566 3507
Fragments arranged by size:
3507 2566 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4997 5171 65
Size: 1183 3749 174 1396
Fragments arranged by size:
3749 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2276 2284 2385 2735 2768 2891 3452
Size: 110 466 8 101 350 33 123 561
Cuts at: 3452 5146 5333 1700
Size: 1694 187 2869
Fragments arranged by size:
2869 1694 561 466 350 187 123 110
101 33 8
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6502
BssHII G'CGCG_C
Cuts at: 1925 2152 2430 2521 1925
Size: 227 278 91 5906
Fragments arranged by size:
5906 278 227 91
BssSI C'ACGA_G
Cuts at: 4562 5574 4562
Size: 1012 5490
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6502
BstBI TT'CG_AA
Cuts at: 5306 5306
Size: 6502
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6502
BstYI r'GATC_y
Cuts at: 1972 2265 3061 3832 4199 4584 4601 6053 6139
Size: 293 796 771 367 385 17 1452 86
Cuts at: 6139 6151 6497 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 796 771 385 367 346 293
86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3067 3067
Size: 6502
BtgI C'CryG_G
Cuts at: 513 1065 2004 2392 2478 2628 3039 3645 3660
Size: 552 939 388 86 150 411 606 15
Cuts at: 3660 513
Size: 3355
Fragments arranged by size:
3355 939 606 552 411 388 150 86
15
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2233 487
Size: 683 56 1007 4756
Fragments arranged by size:
4756 1007 683 56
BtsI GCAGTG_nn'
Cuts at: 2503 3132 4018 4812 4832 2503
Size: 629 886 794 20 4173
Fragments arranged by size:
4173 886 794 629 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6502
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6467
DraI TTT'AAA
Cuts at: 3806 3806
Size: 6502
DraIII CAC_nnn'GTG
Cuts at: 2398 3106 2398
Size: 708 5794
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2658 5509 809
Size: 507 1342 2851 1802
Fragments arranged by size:
2851 1802 1342 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2475 2550 2654
Size: 54 1425 50 275 18 645 75 104
Cuts at: 2654 2733 2745 2826 2889 2893 3042 3949 4838
Size: 79 12 81 63 4 149 907 889
Cuts at: 4838 6467 8
Size: 1629 43
Fragments arranged by size:
1629 1425 907 889 645 275 149 104
81 79 75 63 54 50 43 18
12 4
EagI C'GGCC_G
Cuts at: 1537 1812 2475 2654 2893 3949 1537
Size: 275 663 179 239 1056 4090
Fragments arranged by size:
4090 1056 663 275 239 179
EarI CTCTTCn'nnn_
Cuts at: 1353 3219 3240 3252 3270 3333 3375 3546 3561
Size: 1866 21 12 18 63 42 171 15
Cuts at: 3561 4432 1353
Size: 871 3423
Fragments arranged by size:
3423 1866 871 171 63 42 21 18
15 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2372 5089 5463 5609 387
Size: 1064 138 783 2717 374 146 1280
Fragments arranged by size:
2717 1280 1064 783 374 146 138
EcoICRI GAG'CTC
Cuts at: 3402 3402
Size: 6502
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2162 2999 4565 5162 5949 6217 1239
Size: 393 530 837 1566 597 787 268 1524
Fragments arranged by size:
1566 1524 837 787 597 530 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2084 2084
Size: 6502
EcoO109I rG'GnC_Cy
Cuts at: 2009 2821 2878 3094 2009
Size: 812 57 216 5417
Fragments arranged by size:
5417 812 216 57
EcoRI G'AATT_C
Cuts at: 3811 4189 3811
Size: 378 6124
FseI GG_CCGG'CC
Cuts at: 2895 2895
Size: 6502
FspI TGC'GCA
Cuts at: 2084 2321 5008 2084
Size: 237 2687 3578
Fragments arranged by size:
3578 2687 237
HaeII r_GCGC'y
Cuts at: 1678 2107 2318 2392 2465 2586 2677 5649 1678
Size: 429 211 74 73 121 91 2972 2531
Fragments arranged by size:
2972 2531 429 211 121 91 74 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5143 5175 5217 5249 1373
Size: 32 135 32 3571 32 42 32 2626
Fragments arranged by size:
3571 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3846 4092 669
Size: 988 281 1908 246 3079
Fragments arranged by size:
3079 1908 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6502
HpaI GTT'AAC
Cuts at: 4092 4092
Size: 6502
KasI G'GCGC_C
Cuts at: 2388 2461 2388
Size: 73 6429
KpnI G_GTAC'C
Cuts at: 3676 3827 3676
Size: 151 6351
MfeI C'AATT_G
Cuts at: 4101 4101
Size: 6502
MluI A'CGCG_T
Cuts at: 6378 6378
Size: 6502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2148 2385 5616 5800 1305
Size: 843 237 3231 184 2007
Fragments arranged by size:
3231 2007 843 237 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2828 10
Size: 54 1425 343 996 3684
Fragments arranged by size:
3684 1425 996 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2855 4460 4819 4978 518
Size: 648 1689 1605 359 159 2042
Fragments arranged by size:
2042 1689 1605 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2026 2412 2480 2630 2728 2944 2977 3079
Size: 420 386 68 150 98 216 33 102
Cuts at: 3079 3918 4595 5743 5988 1606
Size: 839 677 1148 245 2120
Fragments arranged by size:
2120 1148 839 677 420 386 245 216
150 102 98 68 33
NaeI GCC'GGC
Cuts at: 2278 2387 2737 2770 2893 2278
Size: 109 350 33 123 5887
Fragments arranged by size:
5887 350 123 109 33
NarI GG'CG_CC
Cuts at: 2389 2462 2389
Size: 73 6429
NcoI C'CATG_G
Cuts at: 513 1065 2004 3039 3645 3660 513
Size: 552 939 1035 606 15 3355
Fragments arranged by size:
3355 1035 939 606 552 15
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6502
NgoMIV G'CCGG_C
Cuts at: 2276 2385 2735 2768 2891 2276
Size: 109 350 33 123 5887
Fragments arranged by size:
5887 350 123 109 33
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6502
NotI GC'GGCC_GC
Cuts at: 3949 3949
Size: 6502
NspI r_CATG'y
Cuts at: 1132 1229 2939 3860 5405 1132
Size: 97 1710 921 1545 2229
Fragments arranged by size:
2229 1710 1545 921 97
PasI CC'CwG_GG
Cuts at: 1348 3436 1348
Size: 2088 4414
PciI A'CATG_T
Cuts at: 5401 5401
Size: 6502
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2062 2367 2398 2601 2824 2914 1108
Size: 954 305 31 203 223 90 4696
Fragments arranged by size:
4696 954 305 223 203 90 31
PfoI T'CCnGG_A
Cuts at: 2034 2055 3513 2034
Size: 21 1458 5023
Fragments arranged by size:
5023 1458 21
PmeI GTTT'AAAC
Cuts at: 3806 3806
Size: 6502
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4571 4603 6084 6116 4571
Size: 32 1481 32 4957
Fragments arranged by size:
4957 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2878 2878
Size: 6502
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6502
PsiI TTA'TAA
Cuts at: 4072 4072
Size: 6502
PspOMI G'GGCC_C
Cuts at: 2008 3432 2008
Size: 1424 5078
PspXI vC'TCGA_Gb
Cuts at: 2234 2234
Size: 6502
PstI C_TGCA'G
Cuts at: 830 2411 2866 3078 3854 830
Size: 1581 455 212 776 3478
Fragments arranged by size:
3478 1581 776 455 212
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6502
PvuII CAG'CTG
Cuts at: 2026 2944 3079 2026
Size: 918 135 5449
Fragments arranged by size:
5449 918 135
SacI G_AGCT'C
Cuts at: 3404 3404
Size: 6502
SacII CC_GC'GG
Cuts at: 2481 2631 2481
Size: 150 6352
SalI G'TCGA_C
Cuts at: 1936 3844 1936
Size: 1908 4594
SbfI CC_TGCA'GG
Cuts at: 3854 3854
Size: 6502
ScaI AGT'ACT
Cuts at: 2834 4750 6462 2834
Size: 1916 1712 2874
Fragments arranged by size:
2874 1916 1712
SfcI C'TryA_G
Cuts at: 826 1045 2407 2862 3074 3850 4985 5666 5857
Size: 219 1362 455 212 776 1135 681 191
Cuts at: 5857 826
Size: 1471
Fragments arranged by size:
1471 1362 1135 776 681 455 219 212
191
SfiI GGCCn_nnn'nGGCC
Cuts at: 2769 2769
Size: 6502
SfoI GGC'GCC
Cuts at: 2390 2463 2390
Size: 73 6429
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6502
SgrAI Cr'CCGG_yG
Cuts at: 1700 2385 1700
Size: 685 5817
SmaI CCC'GGG
Cuts at: 1872 3829 1872
Size: 1957 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2234 2303 3032 3206 4606 5507
Size: 197 926 291 69 729 174 1400 901
Cuts at: 5507 5769 820
Size: 262 1553
Fragments arranged by size:
1553 1400 926 901 729 291 262 197
174 69
SnaBI TAC'GTA
Cuts at: 493 4293 493
Size: 3800 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6502
SphI G_CATG'C
Cuts at: 1132 3860 1132
Size: 2728 3774
SspI AAT'ATT
Cuts at: 5 52 4426 5
Size: 47 4374 2081
Fragments arranged by size:
4374 2081 47
StuI AGG'CCT
Cuts at: 1517 2843 3072 1517
Size: 1326 229 4947
Fragments arranged by size:
4947 1326 229
StyI C'CwwG_G
Cuts at: 513 1065 2004 2799 2844 2913 3039 3645 3660
Size: 552 939 795 45 69 126 606 15
Cuts at: 3660 3941 513
Size: 281 3074
Fragments arranged by size:
3074 939 795 606 552 281 126 69
45 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2684 2831 4529 4546 4699 4884 5329
Size: 1255 1104 147 1698 17 153 185 445
Cuts at: 5329 325
Size: 1498
Fragments arranged by size:
1698 1498 1255 1104 445 185 153 147
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2832 4748 6460
Size: 274 80 33 51 1443 855 1916 1712
Cuts at: 6460 96
Size: 138
Fragments arranged by size:
1916 1712 1443 855 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2037 2069 2956 2988 3479 3511 6462 6494 2037
Size: 32 887 32 491 32 2951 32 2045
Fragments arranged by size:
2951 2045 887 491 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6502
XbaI T'CTAG_A
Cuts at: 3838 3838
Size: 6502
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2368 2821 2368
Size: 453 6049
XhoI C'TCGA_G
Cuts at: 1943 2234 1943
Size: 291 6211
XmaI C'CCGG_G
Cuts at: 1870 3827 1870
Size: 1957 4545
XmnI GAAnn'nnTTC
Cuts at: 4631 4631
Size: 6502
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4310 276
Size: 53 83 186 936 2776 2468
Fragments arranged by size:
2776 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NspI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII
SacI SacII SalI SbfI ScaI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI
BstXI BstZ17I EcoRV FalI FspAI NruI NsiI PacI
PmlI PsrI RsrII SanDI SapI SexAI SrfI SwaI