Restriction map for
pFN21AB9879
(Circular) (Six-base) MAPSORT of: pFN21AB9879.seq Check: 734 from: 1 to: 6457
pFN21AB9879 6457 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4267 278
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3800 1937
Size: 1863 4594
Acc65I G'GTAC_C
Cuts at: 3778 3778
Size: 6457
AclI AA'CG_TT
Cuts at: 1563 4584 4957 1563
Size: 3021 373 3063
Fragments arranged by size:
3063 3021 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2071 2840 3302 4520 5904 6172 1632
Size: 439 769 462 1218 1384 268 1917
Fragments arranged by size:
1917 1384 1218 769 462 439 268
AflII C'TTAA_G
Cuts at: 820 1017 2219 820
Size: 197 1202 5058
Fragments arranged by size:
5058 1202 197
AflIII A'CryG_T
Cuts at: 1203 5356 6333 1203
Size: 4153 977 1327
Fragments arranged by size:
4153 1327 977
AgeI A'CCGG_T
Cuts at: 5288 5288
Size: 6457
AhdI GACnn_n'nnGTC
Cuts at: 5186 5186
Size: 6457
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6457
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2159 2198 2631 1184
Size: 975 39 433 5010
Fragments arranged by size:
5010 975 433 39
AlwNI CAG_nnn'CTG
Cuts at: 2519 2704 4200 5772 2519
Size: 185 1496 1572 3204
Fragments arranged by size:
3204 1572 1496 185
ApaI G_GGCC'C
Cuts at: 2211 2268 3502 3623 2211
Size: 57 1234 121 5045
Fragments arranged by size:
5045 1234 121 57
ApaLI G'TGCA_C
Cuts at: 4514 5670 4514
Size: 1156 5301
ApoI r'AATT_y
Cuts at: 1493 3766 3992 4144 6253 1493
Size: 2273 226 152 2109 1697
Fragments arranged by size:
2273 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 5011 160
Size: 872 3979 1606
Fragments arranged by size:
3979 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
AvaI C'yCGr_G
Cuts at: 1870 1943 2189 2204 3158 3597 3643 3782 1870
Size: 73 246 15 954 439 46 139 4545
Fragments arranged by size:
4545 954 439 246 139 73 46 15
BamHI G'GATC_C
Cuts at: 3787 4154 3787
Size: 367 6090
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2548 2676 3127 3223 3356
Size: 325 217 627 761 128 451 96 133
Cuts at: 3356 3565 3778 5233 618
Size: 209 213 1455 1842
Fragments arranged by size:
1842 1455 761 627 451 325 217 213
209 133 128 96
BanII G_rGCy'C
Cuts at: 1391 2211 2268 3112 3502 3623 3776 1391
Size: 820 57 844 390 121 153 4072
Fragments arranged by size:
4072 844 820 390 153 121 57
BbeI G_GCGC'C
Cuts at: 2680 3131 3360 2680
Size: 451 229 5777
Fragments arranged by size:
5777 451 229
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3543 928
Size: 971 1644 3842
Fragments arranged by size:
3842 1644 971
BbvCI CC'TCA_GC
Cuts at: 2445 2445
Size: 6457
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2771 2805 4648 4682 1903
Size: 34 834 34 1843 34 3678
Fragments arranged by size:
3678 1843 834 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2798 4349 5559 2798
Size: 1551 1210 3696
Fragments arranged by size:
3696 1551 1210
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6457
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2497 3083 5068
Size: 107 122 71 631 605 825 586 1985
Cuts at: 5068 136
Size: 1525
Fragments arranged by size:
1985 1525 825 631 605 586 122 107
71
BglII A'GATC_T
Cuts at: 6452 6452
Size: 6457
BlpI GC'TnA_GC
Cuts at: 3874 3874
Size: 6457
Bme1580I G_kGCm'C
Cuts at: 1790 2211 2268 3264 3502 3560 3570 3623 3662
Size: 421 57 996 238 58 10 53 39
Cuts at: 3662 3684 4518 5674 1790
Size: 22 834 1156 2573
Fragments arranged by size:
2573 1156 996 834 421 238 58 57
53 39 22 10
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6457
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3226 3492 4188
Size: 416 53 83 399 24 1809 266 696
Cuts at: 4188 5146 442
Size: 958 1753
Fragments arranged by size:
1809 1753 958 696 416 399 266 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6457
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2215 2215
Size: 6457
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2408 2784 3744 5117 1239
Size: 1169 376 960 1373 2579
Fragments arranged by size:
2579 1373 1169 960 376
Bpu10I CC'TnA_GC
Cuts at: 2445 2445
Size: 6457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2153 2578 4582 5447 5745 2153
Size: 425 2004 865 298 2865
Fragments arranged by size:
2865 2004 865 425 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3242 5120 882
Size: 617 1743 1878 2219
Fragments arranged by size:
2219 1878 1743 617
BsaAI yAC'GTr
Cuts at: 493 1204 2182 4248 493
Size: 711 978 2066 2702
Fragments arranged by size:
2702 2066 978 711
BsaBI GATnn'nnATC
Cuts at: 6451 6451
Size: 6457
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2021 2677 2868
Size: 53 83 186 936 476 12 656 191
Cuts at: 2868 3015 3128 3357 4264 4646 5281 275
Size: 147 113 229 907 382 635 1451
Fragments arranged by size:
1451 936 907 656 635 476 382 229
191 186 147 113 83 53 12
BsaWI w'CCGG_w
Cuts at: 4890 5288 5562 5709 6120 4890
Size: 398 274 147 411 5227
Fragments arranged by size:
5227 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6232
Fragments arranged by size:
6232 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2447 3212 3364 3664 1186
Size: 1261 765 152 300 3979
Fragments arranged by size:
3979 1261 765 300 152
BseYI C'CCAG_C
Cuts at: 2268 2289 2295 2422 2992 3057 3179 3384 3396
Size: 21 6 127 570 65 122 205 12
Cuts at: 3396 5660 6156 2268
Size: 2264 496 2569
Fragments arranged by size:
2569 2264 570 496 205 127 122 65
21 12 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2036 3369 3541 1163
Size: 573 300 1333 172 4079
Fragments arranged by size:
4079 1333 573 300 172
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3627 3907 4668 5696 1540
Size: 275 186 1626 280 761 1028 2301
Fragments arranged by size:
2301 1626 1028 761 280 275 186
BsiHKAI G_wGCw'C
Cuts at: 2187 4518 4603 5674 2187
Size: 2331 85 1071 2970
Fragments arranged by size:
2970 2331 1071 85
BsmI GAATG_Cn'
Cuts at: 2620 3968 4061 2620
Size: 1348 93 5016
Fragments arranged by size:
5016 1348 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2187 2211 2268 3112 3264 3502 3560
Size: 399 397 24 57 844 152 238 58
Cuts at: 3560 3570 3623 3662 3684 3776 4518 4603 5674
Size: 10 53 39 22 92 742 85 1071
Cuts at: 5674 1391
Size: 2174
Fragments arranged by size:
2174 1071 844 742 399 397 238 152
92 85 58 57 53 39 24 22
10
BspEI T'CCGG_A
Cuts at: 6120 6120
Size: 6457
BspHI T'CATG_A
Cuts at: 4346 6076 4346
Size: 1730 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2061 3812 844
Size: 1217 1751 3489
Fragments arranged by size:
3489 1751 1217
BsrBI CCG'CTC
Cuts at: 1394 1823 2199 3529 3648 4344 1394
Size: 429 376 1330 119 696 3507
Fragments arranged by size:
3507 1330 696 429 376 119
BsrDI GCAATG_nn'
Cuts at: 65 1248 2860 3270 4952 5126 65
Size: 1183 1612 410 1682 174 1396
Fragments arranged by size:
1682 1612 1396 1183 410 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2309 2864 2964 3073 5101 5288 1700
Size: 110 499 555 100 109 2028 187 2869
Fragments arranged by size:
2869 2028 555 499 187 110 109 100
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6457
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6457
BssSI C'ACGA_G
Cuts at: 4517 5529 4517
Size: 1012 5445
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6457
BstBI TT'CG_AA
Cuts at: 5261 5261
Size: 6457
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6457
BstXI CCAn_nnnn'nTGG
Cuts at: 2242 2422 2242
Size: 180 6277
BstYI r'GATC_y
Cuts at: 1972 3787 4154 4539 4556 6008 6094 6106 6452
Size: 1815 367 385 17 1452 86 12 346
Cuts at: 6452 1972
Size: 1977
Fragments arranged by size:
1977 1815 1452 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2066 2073 2439 3042 3378 513
Size: 552 939 62 7 366 603 336 3592
Fragments arranged by size:
3592 939 603 552 366 336 62 7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3386 487
Size: 683 56 2160 3558
Fragments arranged by size:
3558 2160 683 56
BtsI GCAGTG_nn'
Cuts at: 2520 2694 2883 3973 4767 4787 2520
Size: 174 189 1090 794 20 4190
Fragments arranged by size:
4190 1090 794 189 174 20
ClaI AT'CG_AT
Cuts at: 1554 2151 1554
Size: 597 5860
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3700 3735 529
Size: 35 3136 35 3251
Fragments arranged by size:
3251 3136 35 35
DraI TTT'AAA
Cuts at: 3761 3761
Size: 6457
DraIII CAC_nnn'GTG
Cuts at: 3220 3220
Size: 6457
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5464 809
Size: 507 4148 1802
Fragments arranged by size:
4148 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2229 2412 3291
Size: 54 1425 50 275 18 399 183 879
Cuts at: 3291 3624 3904 4793 6422 8
Size: 333 280 889 1629 43
Fragments arranged by size:
1629 1425 889 879 399 333 280 275
183 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3624 3904 1537
Size: 275 1812 280 4090
Fragments arranged by size:
4090 1812 280 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2582 2831 4387 1353
Size: 1229 249 1556 3423
Fragments arranged by size:
3423 1556 1229 249
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2704 3342 5044 5418 5564 387
Size: 1064 138 1115 638 1702 374 146 1280
Fragments arranged by size:
1702 1280 1115 1064 638 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2071 2408 2784 2840 3302 3744 4520
Size: 393 439 337 376 56 462 442 776
Cuts at: 4520 5117 5904 6172 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 787 776 597 462 442 439 393
376 337 268 56
EcoNI CCTnn'n_nnAGG
Cuts at: 3327 3327
Size: 6457
EcoO109I rG'GnC_Cy
Cuts at: 2208 2239 2264 2265 2403 2460 3049 3319 3338
Size: 31 25 1 138 57 589 270 19
Cuts at: 3338 3498 3499 3619 3751 2208
Size: 160 1 120 132 4914
Fragments arranged by size:
4914 589 270 160 138 132 120 57
31 25 19 1 1
EcoRI G'AATT_C
Cuts at: 3766 4144 3766
Size: 378 6079
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3601 3601
Size: 6457
FspI TGC'GCA
Cuts at: 4963 4963
Size: 6457
HaeII r_GCGC'y
Cuts at: 1678 2680 2711 3131 3360 5604 1678
Size: 1002 31 420 229 2244 2531
Fragments arranged by size:
2531 2244 1002 420 229 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 5098 5130 5172 5204 1373
Size: 32 135 32 3526 32 42 32 2626
Fragments arranged by size:
3526 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3801 4047 669
Size: 988 281 1863 246 3079
Fragments arranged by size:
3079 1863 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6457
HpaI GTT'AAC
Cuts at: 4047 4047
Size: 6457
KasI G'GCGC_C
Cuts at: 2676 3127 3356 2676
Size: 451 229 5777
Fragments arranged by size:
5777 451 229
KpnI G_GTAC'C
Cuts at: 3782 3782
Size: 6457
MfeI C'AATT_G
Cuts at: 4056 4056
Size: 6457
MluI A'CGCG_T
Cuts at: 6333 6333
Size: 6457
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5571 5755 1305
Size: 4266 184 2007
Fragments arranged by size:
4266 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2414 10
Size: 54 1425 343 582 4053
Fragments arranged by size:
4053 1425 582 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4415 4774 4933 518
Size: 648 3249 359 159 2042
Fragments arranged by size:
3249 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2272 2510 2992 3044 3245 3446 3465 3477
Size: 666 238 482 52 201 201 19 12
Cuts at: 3477 3664 3873 4550 5698 5943 1606
Size: 187 209 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 666 482 245 238 209
201 201 187 52 19 12
NaeI GCC'GGC
Cuts at: 2866 2966 3075 2866
Size: 100 109 6248
Fragments arranged by size:
6248 109 100
NarI GG'CG_CC
Cuts at: 2677 3128 3357 2677
Size: 451 229 5777
Fragments arranged by size:
5777 451 229
NcoI C'CATG_G
Cuts at: 513 1065 2004 2073 513
Size: 552 939 69 4897
Fragments arranged by size:
4897 939 552 69
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6457
NgoMIV G'CCGG_C
Cuts at: 2864 2964 3073 2864
Size: 100 109 6248
Fragments arranged by size:
6248 109 100
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6457
NotI GC'GGCC_GC
Cuts at: 3904 3904
Size: 6457
NspI r_CATG'y
Cuts at: 1132 1229 3815 5360 1132
Size: 97 2586 1545 2229
Fragments arranged by size:
2586 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2495 3301 3494 3684 1348
Size: 1147 806 193 190 4121
Fragments arranged by size:
4121 1147 806 193 190
PciI A'CATG_T
Cuts at: 5356 5356
Size: 6457
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2079 1108
Size: 971 5486
PfoI T'CCnGG_A
Cuts at: 2800 3116 2800
Size: 316 6141
PmeI GTTT'AAAC
Cuts at: 3761 3761
Size: 6457
PmlI CAC'GTG
Cuts at: 2182 2182
Size: 6457
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4526 4558 6039 6071 4526
Size: 32 1481 32 4912
Fragments arranged by size:
4912 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2239 2403 3319 3751 2239
Size: 164 916 432 4945
Fragments arranged by size:
4945 916 432 164
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6457
PsiI TTA'TAA
Cuts at: 4027 4027
Size: 6457
PspOMI G'GGCC_C
Cuts at: 2207 2264 3498 3619 2207
Size: 57 1234 121 5045
Fragments arranged by size:
5045 1234 121 57
PstI C_TGCA'G
Cuts at: 830 2515 3370 3809 830
Size: 1685 855 439 3478
Fragments arranged by size:
3478 1685 855 439
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6457
PvuII CAG'CTG
Cuts at: 2272 2510 2992 2272
Size: 238 482 5737
Fragments arranged by size:
5737 482 238
RsrII CG'GwC_CG
Cuts at: 2131 2131
Size: 6457
SacII CC_GC'GG
Cuts at: 3045 3045
Size: 6457
SalI G'TCGA_C
Cuts at: 1936 3799 1936
Size: 1863 4594
SanDI GG'GwC_CC
Cuts at: 2403 3751 2403
Size: 1348 5109
SapI GCTCTTCn'nnn_
Cuts at: 2831 2831
Size: 6457
SbfI CC_TGCA'GG
Cuts at: 3809 3809
Size: 6457
ScaI AGT'ACT
Cuts at: 4705 6417 4705
Size: 1712 4745
SfcI C'TryA_G
Cuts at: 826 1045 2511 3366 3805 4940 5621 5812 826
Size: 219 1466 855 439 1135 681 191 1471
Fragments arranged by size:
1471 1466 1135 855 681 439 219 191
SfoI GGC'GCC
Cuts at: 2678 3129 3358 2678
Size: 451 229 5777
Fragments arranged by size:
5777 451 229
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6457
SgrAI Cr'CCGG_yG
Cuts at: 1700 2864 1700
Size: 1164 5293
SmaI CCC'GGG
Cuts at: 1872 2206 3599 3784 1872
Size: 334 1393 185 4545
Fragments arranged by size:
4545 1393 334 185
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2168 2219 2557 4561 5462 5724
Size: 197 926 225 51 338 2004 901 262
Cuts at: 5724 820
Size: 1553
Fragments arranged by size:
2004 1553 926 901 338 262 225 197
51
SnaBI TAC'GTA
Cuts at: 493 4248 493
Size: 3755 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6457
SphI G_CATG'C
Cuts at: 1132 3815 1132
Size: 2683 3774
SrfI GCCC'GGGC
Cuts at: 2206 2206
Size: 6457
SspI AAT'ATT
Cuts at: 5 52 4381 5
Size: 47 4329 2081
Fragments arranged by size:
4329 2081 47
StuI AGG'CCT
Cuts at: 1517 2538 1517
Size: 1021 5436
StyI C'CwwG_G
Cuts at: 513 1065 2004 2073 2235 3315 3896 513
Size: 552 939 69 162 1080 581 3074
Fragments arranged by size:
3074 1080 939 581 552 162 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3208 3328 3557 4484 4501 4654 4839
Size: 1255 1628 120 229 927 17 153 185
Cuts at: 4839 5284 325
Size: 445 1498
Fragments arranged by size:
1628 1498 1255 927 445 229 185 153
120 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2391 3636 4703
Size: 274 80 33 51 1443 414 1245 1067
Cuts at: 4703 6415 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1245 1067 414 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6417 6449 6417
Size: 32 6425
Tth111I GACn'n_nGTC
Cuts at: 1316 3014 1316
Size: 1698 4759
XbaI T'CTAG_A
Cuts at: 3793 3793
Size: 6457
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2239 2239
Size: 6457
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6457
XmaI C'CCGG_G
Cuts at: 1870 2204 3597 3782 1870
Size: 334 1393 185 4545
Fragments arranged by size:
4545 1393 334 185
XmnI GAAnn'nnTTC
Cuts at: 2825 4586 2825
Size: 1761 4696
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4265 276
Size: 53 83 186 936 2731 2468
Fragments arranged by size:
2731 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI
BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI
NsiI PacI PspXI PsrI SacI SexAI SfiI SwaI