Restriction map for
pFN21AB9878
(Circular) (Six-base) MAPSORT of: pFN21AB9878.seq Check: 221 from: 1 to: 6430
pFN21AB9878 6430 bp
With 182 enzymes: SgfI *
August 20, 2012 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2193 3677 2193
Size: 1484 4946
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2813 4240 278
Size: 53 83 186 936 1277 1427 2468
Fragments arranged by size:
2468 1427 1277 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3252 3773 1937
Size: 1315 521 4594
Fragments arranged by size:
4594 1315 521
Acc65I G'GTAC_C
Cuts at: 3035 3751 3035
Size: 716 5714
AclI AA'CG_TT
Cuts at: 1563 4557 4930 1563
Size: 2994 373 3063
Fragments arranged by size:
3063 2994 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2381 3197 3264 3519 4493 5877 6145 1632
Size: 749 816 67 255 974 1384 268 1917
Fragments arranged by size:
1917 1384 974 816 749 268 255 67
AfeI AGC'GCT
Cuts at: 3625 3625
Size: 6430
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6233
AflIII A'CryG_T
Cuts at: 1203 5329 6306 1203
Size: 4126 977 1327
Fragments arranged by size:
4126 1327 977
AgeI A'CCGG_T
Cuts at: 5261 5261
Size: 6430
AhdI GACnn_n'nnGTC
Cuts at: 5159 5159
Size: 6430
AleI CACnn'nnGTG
Cuts at: 1166 2108 1166
Size: 942 5488
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6430
AlwNI CAG_nnn'CTG
Cuts at: 4173 5745 4173
Size: 1572 4858
ApaI G_GGCC'C
Cuts at: 2221 2221
Size: 6430
ApaLI G'TGCA_C
Cuts at: 4487 5643 4487
Size: 1156 5274
ApoI r'AATT_y
Cuts at: 1493 3739 3965 4117 6226 1493
Size: 2246 226 152 2109 1697
Fragments arranged by size:
2246 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4984 160
Size: 872 3952 1606
Fragments arranged by size:
3952 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
AvaI C'yCGr_G
Cuts at: 1870 1943 2174 2381 2684 2703 3164 3755 1870
Size: 73 231 207 303 19 461 591 4545
Fragments arranged by size:
4545 591 461 303 231 207 73 19
BamHI G'GATC_C
Cuts at: 3760 4127 3760
Size: 367 6063
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2096 2170 3035 3751 5206
Size: 325 217 627 309 74 865 716 1455
Cuts at: 5206 618
Size: 1842
Fragments arranged by size:
1842 1455 865 716 627 325 309 217
74
BanII G_rGCy'C
Cuts at: 1391 2133 2221 2693 3549 3749 1391
Size: 742 88 472 856 200 4072
Fragments arranged by size:
4072 856 742 472 200 88
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2369 3109 928
Size: 971 470 740 4249
Fragments arranged by size:
4249 971 740 470
BbvCI CC'TCA_GC
Cuts at: 2018 2047 2073 2018
Size: 29 26 6375
Fragments arranged by size:
6375 29 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2698 2732 4621 4655 1903
Size: 34 761 34 1889 34 3678
Fragments arranged by size:
3678 1889 761 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4322 5532 4322
Size: 1210 5220
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6430
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5041 136
Size: 107 122 71 631 605 3369 1525
Fragments arranged by size:
3369 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6425 6425
Size: 6430
BlpI GC'TnA_GC
Cuts at: 3847 3847
Size: 6430
Bme1580I G_kGCm'C
Cuts at: 1790 2101 2175 2221 2532 4491 5647 1790
Size: 311 74 46 311 1959 1156 2573
Fragments arranged by size:
2573 1959 1156 311 311 74 46
BmgBI CAC'GTC
Cuts at: 1318 2847 1318
Size: 1529 4901
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3353 3490 4161
Size: 416 53 83 399 24 1936 137 671
Cuts at: 4161 5119 442
Size: 958 1753
Fragments arranged by size:
1936 1753 958 671 416 399 137 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6430
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2210 2783 2854 3226 5090 1239
Size: 971 573 71 372 1864 2579
Fragments arranged by size:
2579 1864 971 573 372 71
Bpu10I CC'TnA_GC
Cuts at: 2018 2047 2073 2018
Size: 29 26 6375
Fragments arranged by size:
6375 29 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2211 4555 5420 5718 2211
Size: 2344 865 298 2923
Fragments arranged by size:
2923 2344 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5093 882
Size: 617 3594 2219
Fragments arranged by size:
3594 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3508 4221 493
Size: 711 2304 713 2702
Fragments arranged by size:
2702 2304 713 711
BsaBI GATnn'nnATC
Cuts at: 6424 6424
Size: 6430
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2475 2810 3182 4237
Size: 53 83 186 936 942 335 372 1055
Cuts at: 4237 4619 5254 275
Size: 382 635 1451
Fragments arranged by size:
1451 1055 942 936 635 382 372 335
186 83 53
BsaWI w'CCGG_w
Cuts at: 4863 5261 5535 5682 6093 4863
Size: 398 274 147 411 5200
Fragments arranged by size:
5200 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2528 2558 2891 2921 1178
Size: 30 165 30 1125 30 333 30 4687
Fragments arranged by size:
4687 1125 333 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2061 2564 2591 2636 2639 3520 3557 1186
Size: 875 503 27 45 3 881 37 4059
Fragments arranged by size:
4059 881 875 503 45 37 27 3
BseYI C'CCAG_C
Cuts at: 2080 2392 2465 2569 3196 5633 6129 2080
Size: 312 73 104 627 2437 496 2381
Fragments arranged by size:
2437 2381 627 496 312 104 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3655 1163
Size: 573 1919 3938
Fragments arranged by size:
3938 1919 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2257 2289 2455 2519 3880 4641
Size: 275 186 256 32 166 64 1361 761
Cuts at: 4641 5669 1540
Size: 1028 2301
Fragments arranged by size:
2301 1361 1028 761 275 256 186 166
64 32
BsiHKAI G_wGCw'C
Cuts at: 2693 3549 4491 4576 5647 2693
Size: 856 942 85 1071 3476
Fragments arranged by size:
3476 1071 942 856 85
BsmI GAATG_Cn'
Cuts at: 3941 4034 3941
Size: 93 6337
BsmBI CGTCTCn'nnnn_
Cuts at: 2345 2378 2759 2894 2345
Size: 33 381 135 5881
Fragments arranged by size:
5881 381 135 33
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2101 2133 2175 2221 2532 2693 3549
Size: 399 311 32 42 46 311 161 856
Cuts at: 3549 3749 4491 4576 5647 1391
Size: 200 742 85 1071 2174
Fragments arranged by size:
2174 1071 856 742 399 311 311 200
161 85 46 42 32
BspEI T'CCGG_A
Cuts at: 6093 6093
Size: 6430
BspHI T'CATG_A
Cuts at: 2985 4319 6049 2985
Size: 1334 1730 3366
Fragments arranged by size:
3366 1730 1334
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2193 3677 3785 844
Size: 1349 1484 108 3489
Fragments arranged by size:
3489 1484 1349 108
BsrBI CCG'CTC
Cuts at: 1394 1823 2431 2629 4317 1394
Size: 429 608 198 1688 3507
Fragments arranged by size:
3507 1688 608 429 198
BsrDI GCAATG_nn'
Cuts at: 65 1248 3067 3422 4925 5099 65
Size: 1183 1819 355 1503 174 1396
Fragments arranged by size:
1819 1503 1396 1183 355 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2284 2460 2514 2518 2698 2834 3404
Size: 110 474 176 54 4 180 136 570
Cuts at: 3404 5074 5261 1700
Size: 1670 187 2869
Fragments arranged by size:
2869 1670 570 474 187 180 176 136
110 54 4
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6430
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6430
BssSI C'ACGA_G
Cuts at: 4490 5502 4490
Size: 1012 5418
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6430
BstBI TT'CG_AA
Cuts at: 2826 2928 5234 2826
Size: 102 2306 4022
Fragments arranged by size:
4022 2306 102
BstEII G'GTnAC_C
Cuts at: 1184 3322 1184
Size: 2138 4292
BstXI CCAn_nnnn'nTGG
Cuts at: 3082 3082
Size: 6430
BstYI r'GATC_y
Cuts at: 1972 2553 3159 3386 3760 4127 4512 4529 5981
Size: 581 606 227 374 367 385 17 1452
Cuts at: 5981 6067 6079 6425 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 606 581 385 374 367 346
227 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 3092 3092
Size: 6430
BtgI C'CryG_G
Cuts at: 513 1065 2004 2116 2166 2289 2583 513
Size: 552 939 112 50 123 294 4360
Fragments arranged by size:
4360 939 552 294 123 112 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2695 3385 3403 487
Size: 683 56 1469 690 18 3514
Fragments arranged by size:
3514 1469 690 683 56 18
BtsI GCAGTG_nn'
Cuts at: 2279 3324 3660 3946 4740 4760 2279
Size: 1045 336 286 794 20 3949
Fragments arranged by size:
3949 1045 794 336 286 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6430
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6395
DraI TTT'AAA
Cuts at: 3734 3734
Size: 6430
DraIII CAC_nnn'GTG
Cuts at: 3361 3361
Size: 6430
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5437 809
Size: 507 4121 1802
Fragments arranged by size:
4121 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2202 2286 2516
Size: 54 1425 50 275 18 372 84 230
Cuts at: 2516 3006 3045 3084 3877 4766 6395 8
Size: 490 39 39 793 889 1629 43
Fragments arranged by size:
1629 1425 889 793 490 372 275 230
84 54 50 43 39 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2286 2516 3877 1537
Size: 275 474 230 1361 4090
Fragments arranged by size:
4090 1361 474 275 230
EarI CTCTTCn'nnn_
Cuts at: 1353 2678 3480 3538 3597 4360 1353
Size: 1325 802 58 59 763 3423
Fragments arranged by size:
3423 1325 802 763 59 58
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2501 3092 5017 5391 5537 387
Size: 1064 138 912 591 1925 374 146 1280
Fragments arranged by size:
1925 1280 1064 912 591 374 146 138
EcoICRI GAG'CTC
Cuts at: 2691 3547 2691
Size: 856 5574
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2210 2381 2783 2854 3197 3226 3264
Size: 393 578 171 402 71 343 29 38
Cuts at: 3264 3519 4493 5090 5877 6145 1239
Size: 255 974 597 787 268 1524
Fragments arranged by size:
1524 974 787 597 578 402 393 343
268 255 171 71 38 29
EcoNI CCTnn'n_nnAGG
Cuts at: 2618 2618
Size: 6430
EcoO109I rG'GnC_Cy
Cuts at: 2217 2218 2853 2217
Size: 1 635 5794
Fragments arranged by size:
5794 635 1
EcoRI G'AATT_C
Cuts at: 3739 4117 3739
Size: 378 6052
FseI GG_CCGG'CC
Cuts at: 2702 2702
Size: 6430
FspI TGC'GCA
Cuts at: 4936 4936
Size: 6430
HaeII r_GCGC'y
Cuts at: 1678 2017 2080 2659 3627 5577 1678
Size: 339 63 579 968 1950 2531
Fragments arranged by size:
2531 1950 968 579 339 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2528 2560 3192 3224 5071
Size: 32 135 32 956 32 632 32 1847
Cuts at: 5071 5103 5145 5177 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1847 956 632 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2524 3703 3774 4020 669
Size: 988 281 586 1179 71 246 3079
Fragments arranged by size:
3079 1179 988 586 281 246 71
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6430
HpaI GTT'AAC
Cuts at: 4020 4020
Size: 6430
KpnI G_GTAC'C
Cuts at: 3039 3755 3039
Size: 716 5714
MfeI C'AATT_G
Cuts at: 4029 4029
Size: 6430
MluI A'CGCG_T
Cuts at: 6306 6306
Size: 6430
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3471 3564 5544 5728 1305
Size: 2166 93 1980 184 2007
Fragments arranged by size:
2166 2007 1980 184 93
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3008 3086 10
Size: 54 1425 343 1176 78 3354
Fragments arranged by size:
3354 1425 1176 343 78 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2108 2165 3506 4388 4747 4906 518
Size: 648 942 57 1341 882 359 159 2042
Fragments arranged by size:
2042 1341 942 882 648 359 159 57
MspA1I CmG'CkG
Cuts at: 1606 2091 2269 2291 2359 2569 3846 4523 5671
Size: 485 178 22 68 210 1277 677 1148
Cuts at: 5671 5916 1606
Size: 245 2120
Fragments arranged by size:
2120 1277 1148 677 485 245 210 178
68 22
NaeI GCC'GGC
Cuts at: 2286 2516 2700 2836 2286
Size: 230 184 136 5880
Fragments arranged by size:
5880 230 184 136
NcoI C'CATG_G
Cuts at: 513 1065 2004 2583 513
Size: 552 939 579 4360
Fragments arranged by size:
4360 939 579 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6430
NgoMIV G'CCGG_C
Cuts at: 2284 2514 2698 2834 2284
Size: 230 184 136 5880
Fragments arranged by size:
5880 230 184 136
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6430
NotI GC'GGCC_GC
Cuts at: 3877 3877
Size: 6430
NruI TCG'CGA
Cuts at: 2977 2977
Size: 6430
NspI r_CATG'y
Cuts at: 1132 1229 3505 3788 5333 1132
Size: 97 2276 283 1545 2229
Fragments arranged by size:
2276 2229 1545 283 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6430
PciI A'CATG_T
Cuts at: 5329 5329
Size: 6430
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2107 2188 1108
Size: 999 81 5350
Fragments arranged by size:
5350 999 81
PfoI T'CCnGG_A
Cuts at: 3297 3297
Size: 6430
PmeI GTTT'AAAC
Cuts at: 3734 3734
Size: 6430
PmlI CAC'GTG
Cuts at: 3508 3508
Size: 6430
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4499 4531 6012 6044 4499
Size: 32 1481 32 4885
Fragments arranged by size:
4885 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2853 2853
Size: 6430
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6430
PsiI TTA'TAA
Cuts at: 4000 4000
Size: 6430
PspOMI G'GGCC_C
Cuts at: 2217 2217
Size: 6430
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2105 2137 2105
Size: 32 6398
PstI C_TGCA'G
Cuts at: 830 3159 3782 830
Size: 2329 623 3478
Fragments arranged by size:
3478 2329 623
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6430
PvuII CAG'CTG
Cuts at: 2359 2569 2359
Size: 210 6220
SacI G_AGCT'C
Cuts at: 2693 3549 2693
Size: 856 5574
SacII CC_GC'GG
Cuts at: 2292 2292
Size: 6430
SalI G'TCGA_C
Cuts at: 1936 3772 1936
Size: 1836 4594
SanDI GG'GwC_CC
Cuts at: 2853 2853
Size: 6430
SapI GCTCTTCn'nnn_
Cuts at: 2678 3480 2678
Size: 802 5628
SbfI CC_TGCA'GG
Cuts at: 3782 3782
Size: 6430
ScaI AGT'ACT
Cuts at: 2444 4678 6390 2444
Size: 2234 1712 2484
Fragments arranged by size:
2484 2234 1712
SfcI C'TryA_G
Cuts at: 826 1045 3155 3778 4913 5594 5785 826
Size: 219 2110 623 1135 681 191 1471
Fragments arranged by size:
2110 1471 1135 681 623 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6430
SgrAI Cr'CCGG_yG
Cuts at: 1700 3404 1700
Size: 1704 4726
SmaI CCC'GGG
Cuts at: 1872 2176 2705 3757 1872
Size: 304 529 1052 4545
Fragments arranged by size:
4545 1052 529 304
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2226 4534 5435 5697 820
Size: 197 926 283 2308 901 262 1553
Fragments arranged by size:
2308 1553 926 901 283 262 197
SnaBI TAC'GTA
Cuts at: 493 4221 493
Size: 3728 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6430
SphI G_CATG'C
Cuts at: 1132 3788 1132
Size: 2656 3774
SspI AAT'ATT
Cuts at: 5 52 4354 5
Size: 47 4302 2081
Fragments arranged by size:
4302 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6430
StyI C'CwwG_G
Cuts at: 513 1065 2004 2583 3147 3869 513
Size: 552 939 579 564 722 3074
Fragments arranged by size:
3074 939 722 579 564 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3372 4457 4474 4627 4812 5257 325
Size: 1255 1792 1085 17 153 185 445 1498
Fragments arranged by size:
1792 1498 1255 1085 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2442 3498 4676
Size: 274 80 33 51 1443 465 1056 1178
Cuts at: 4676 6388 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1178 1056 465 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2891 2923 6390 6422 2891
Size: 32 3467 32 2899
Fragments arranged by size:
3467 2899 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6430
XbaI T'CTAG_A
Cuts at: 3766 3766
Size: 6430
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3193 3334 3193
Size: 141 6289
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6430
XmaI C'CCGG_G
Cuts at: 1870 2174 2703 3755 1870
Size: 304 529 1052 4545
Fragments arranged by size:
4545 1052 529 304
XmnI GAAnn'nnTTC
Cuts at: 4559 4559
Size: 6430
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2811 4238 276
Size: 53 83 186 936 1277 1427 2468
Fragments arranged by size:
2468 1427 1277 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI
BstZ17I EcoRV FalI FspAI KasI NarI NsiI PacI
PspXI RsrII SexAI SfiI SfoI SrfI SwaI