Restriction map for
pFN21AB9875
(Circular) (Six-base) MAPSORT of: pFN21AB9875.seq Check: 9263 from: 1 to: 6286
pFN21AB9875 6286 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4096 278
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3629 1937
Size: 1692 4594
Acc65I G'GTAC_C
Cuts at: 3295 3607 3295
Size: 312 5974
AclI AA'CG_TT
Cuts at: 1563 4413 4786 1563
Size: 2850 373 3063
Fragments arranged by size:
3063 2850 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2195 2427 2588 3078 3168 4349 5733 6001
Size: 563 232 161 490 90 1181 1384 268
Cuts at: 6001 1632
Size: 1917
Fragments arranged by size:
1917 1384 1181 563 490 268 232 161
90
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6089
AflIII A'CryG_T
Cuts at: 1203 5185 6162 1203
Size: 3982 977 1327
Fragments arranged by size:
3982 1327 977
AgeI A'CCGG_T
Cuts at: 5117 5117
Size: 6286
AhdI GACnn_n'nnGTC
Cuts at: 5015 5015
Size: 6286
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6286
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2774 1184
Size: 1590 4696
AlwNI CAG_nnn'CTG
Cuts at: 4029 5601 4029
Size: 1572 4714
ApaLI G'TGCA_C
Cuts at: 4343 5499 4343
Size: 1156 5130
ApoI r'AATT_y
Cuts at: 1493 2071 2567 2813 2898 3152 3197 3595 3821
Size: 578 496 246 85 254 45 398 226
Cuts at: 3821 3973 6082 1493
Size: 152 2109 1697
Fragments arranged by size:
2109 1697 578 496 398 254 246 226
152 85 45
AseI AT'TA_AT
Cuts at: 160 1032 2430 4840 160
Size: 872 1398 2410 1606
Fragments arranged by size:
2410 1606 1398 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
AvaI C'yCGr_G
Cuts at: 1870 1943 3611 1870
Size: 73 1668 4545
Fragments arranged by size:
4545 1668 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 3279 3312 3279
Size: 33 6253
BamHI G'GATC_C
Cuts at: 3616 3983 3616
Size: 367 5919
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3295 3607 5062 618
Size: 325 217 627 1508 312 1455 1842
Fragments arranged by size:
1842 1508 1455 627 325 312 217
BanII G_rGCy'C
Cuts at: 1391 3318 3466 3605 1391
Size: 1927 148 139 4072
Fragments arranged by size:
4072 1927 148 139
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3147 928
Size: 971 1248 4067
Fragments arranged by size:
4067 1248 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4477 4511 1903
Size: 34 2540 34 3678
Fragments arranged by size:
3678 2540 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4178 5388 4178
Size: 1210 5076
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6286
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4897 136
Size: 107 122 71 631 605 3225 1525
Fragments arranged by size:
3225 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6281 6281
Size: 6286
BlpI GC'TnA_GC
Cuts at: 3703 3703
Size: 6286
Bme1580I G_kGCm'C
Cuts at: 1790 3360 4347 5503 1790
Size: 1570 987 1156 2573
Fragments arranged by size:
2573 1570 1156 987
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6286
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3370 4017 4975
Size: 416 53 83 399 24 1953 647 958
Cuts at: 4975 442
Size: 1753
Fragments arranged by size:
1953 1753 958 647 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2154 2764 4946 1239
Size: 915 610 2182 2579
Fragments arranged by size:
2579 2182 915 610
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4411 5276 5574 4411
Size: 865 298 5123
Fragments arranged by size:
5123 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2906 4949 882
Size: 617 1407 2043 2219
Fragments arranged by size:
2219 2043 1407 617
BsaAI yAC'GTr
Cuts at: 493 1204 4077 493
Size: 711 2873 2702
Fragments arranged by size:
2873 2702 711
BsaBI GATnn'nnATC
Cuts at: 6280 6280
Size: 6286
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4093 4475 5110 275
Size: 53 83 186 936 2560 382 635 1451
Fragments arranged by size:
2560 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4719 5117 5391 5538 5949 4719
Size: 398 274 147 411 5056
Fragments arranged by size:
5056 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6061
Fragments arranged by size:
6061 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2205 1186
Size: 1019 5267
BseYI C'CCAG_C
Cuts at: 2227 2737 5489 5985 2227
Size: 510 2752 496 2528
Fragments arranged by size:
2752 2528 510 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2359 3186 1163
Size: 573 623 827 4263
Fragments arranged by size:
4263 827 623 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3736 4497 5525 1540
Size: 275 186 1735 761 1028 2301
Fragments arranged by size:
2301 1735 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3318 3466 4347 4432 5503 3318
Size: 148 881 85 1071 4101
Fragments arranged by size:
4101 1071 881 148 85
BsmI GAATG_Cn'
Cuts at: 3797 3890 3797
Size: 93 6193
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3318 3360 3466 3605 4347 4432 5503
Size: 399 1528 42 106 139 742 85 1071
Cuts at: 5503 1391
Size: 2174
Fragments arranged by size:
2174 1528 1071 742 399 139 106 85
42
BspEI T'CCGG_A
Cuts at: 5949 5949
Size: 6286
BspHI T'CATG_A
Cuts at: 4175 5905 4175
Size: 1730 4556
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3641 844
Size: 2797 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4173 1394
Size: 429 2350 3507
Fragments arranged by size:
3507 2350 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3445 4781 4955 65
Size: 1183 2197 1336 174 1396
Fragments arranged by size:
2197 1396 1336 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4930 5117 1700
Size: 110 3120 187 2869
Fragments arranged by size:
3120 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 2439 2703 96
Size: 2343 264 3679
Fragments arranged by size:
3679 2343 264
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6286
BssSI C'ACGA_G
Cuts at: 4346 5358 4346
Size: 1012 5274
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6286
BstBI TT'CG_AA
Cuts at: 5090 5090
Size: 6286
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6286
BstXI CCAn_nnnn'nTGG
Cuts at: 2434 2434
Size: 6286
BstYI r'GATC_y
Cuts at: 1972 2120 3115 3382 3616 3983 4368 4385 5837
Size: 148 995 267 234 367 385 17 1452
Cuts at: 5837 5923 5935 6281 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 995 385 367 346 267 234
148 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2284 513
Size: 552 1219 4515
Fragments arranged by size:
4515 1219 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5547
Fragments arranged by size:
5547 683 56
BtsI GCAGTG_nn'
Cuts at: 3802 4596 4616 3802
Size: 794 20 5472
Fragments arranged by size:
5472 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3118 3153 529
Size: 35 2554 35 3662
Fragments arranged by size:
3662 2554 35 35
DraI TTT'AAA
Cuts at: 3590 3590
Size: 6286
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5293 809
Size: 507 3977 1802
Fragments arranged by size:
3977 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3733 4622 6251
Size: 54 1425 50 275 18 1903 889 1629
Cuts at: 6251 8
Size: 43
Fragments arranged by size:
1903 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3733 1537
Size: 275 1921 4090
Fragments arranged by size:
4090 1921 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2223 3069 4216 1353
Size: 870 846 1147 3423
Fragments arranged by size:
3423 1147 870 846
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4873 5247 5393 387
Size: 1064 138 3284 374 146 1280
Fragments arranged by size:
3284 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 3316 3464 3316
Size: 148 6138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2154 2195 2427 2588 2764 3078 3168
Size: 393 522 41 232 161 176 314 90
Cuts at: 3168 4349 4946 5733 6001 1239
Size: 1181 597 787 268 1524
Fragments arranged by size:
1524 1181 787 597 522 393 314 268
232 176 161 90 41
EcoRI G'AATT_C
Cuts at: 3595 3973 3595
Size: 378 5908
EcoRV GAT'ATC
Cuts at: 3283 3283
Size: 6286
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3580 3580
Size: 6286
FspI TGC'GCA
Cuts at: 4792 4792
Size: 6286
HaeII r_GCGC'y
Cuts at: 1678 5433 1678
Size: 3755 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2600 2632 4927 4959 5001
Size: 32 135 32 1028 32 2295 32 42
Cuts at: 5001 5033 1373
Size: 32 2626
Fragments arranged by size:
2626 2295 1028 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3630 3876 669
Size: 988 281 1692 246 3079
Fragments arranged by size:
3079 1692 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6286
HpaI GTT'AAC
Cuts at: 3876 3876
Size: 6286
KpnI G_GTAC'C
Cuts at: 3299 3611 3299
Size: 312 5974
MfeI C'AATT_G
Cuts at: 3885 3885
Size: 6286
MluI A'CGCG_T
Cuts at: 6162 6162
Size: 6286
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5400 5584 1305
Size: 4095 184 2007
Fragments arranged by size:
4095 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4464
Fragments arranged by size:
4464 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2148 2163 2432 3351 4244 4603 4762
Size: 648 982 15 269 919 893 359 159
Cuts at: 4762 518
Size: 2042
Fragments arranged by size:
2042 982 919 893 648 359 269 159
15
MspA1I CmG'CkG
Cuts at: 1606 2741 3702 4379 5527 5772 1606
Size: 1135 961 677 1148 245 2120
Fragments arranged by size:
2120 1148 1135 961 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2284 513
Size: 552 1219 4515
Fragments arranged by size:
4515 1219 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6286
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6286
NotI GC'GGCC_GC
Cuts at: 3733 3733
Size: 6286
NspI r_CATG'y
Cuts at: 1132 1229 2491 3261 3449 3644 5189 1132
Size: 97 1262 770 188 195 1545 2229
Fragments arranged by size:
2229 1545 1262 770 195 188 97
PasI CC'CwG_GG
Cuts at: 1348 2728 1348
Size: 1380 4906
PciI A'CATG_T
Cuts at: 5185 5185
Size: 6286
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6286
PmeI GTTT'AAAC
Cuts at: 3590 3590
Size: 6286
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3384 3416 4355 4387 5868 5900 3384
Size: 32 939 32 1481 32 3770
Fragments arranged by size:
3770 1481 939 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6286
PsiI TTA'TAA
Cuts at: 3856 3856
Size: 6286
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 2385 2417 3514 3546 2385
Size: 32 1097 32 5125
Fragments arranged by size:
5125 1097 32 32
PstI C_TGCA'G
Cuts at: 830 2016 3638 830
Size: 1186 1622 3478
Fragments arranged by size:
3478 1622 1186
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6286
PvuII CAG'CTG
Cuts at: 2741 2741
Size: 6286
SacI G_AGCT'C
Cuts at: 3318 3466 3318
Size: 148 6138
SalI G'TCGA_C
Cuts at: 1936 3628 1936
Size: 1692 4594
SapI GCTCTTCn'nnn_
Cuts at: 3069 3069
Size: 6286
SbfI CC_TGCA'GG
Cuts at: 3638 3638
Size: 6286
ScaI AGT'ACT
Cuts at: 4534 6246 4534
Size: 1712 4574
SfcI C'TryA_G
Cuts at: 826 1045 2012 3634 4769 5450 5641 826
Size: 219 967 1622 1135 681 191 1471
Fragments arranged by size:
1622 1471 1135 967 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6286
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6286
SmaI CCC'GGG
Cuts at: 1872 3613 1872
Size: 1741 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4390 5291 5553 820
Size: 197 926 2447 901 262 1553
Fragments arranged by size:
2447 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4077 493
Size: 3584 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6286
SphI G_CATG'C
Cuts at: 1132 3644 1132
Size: 2512 3774
SspI AAT'ATT
Cuts at: 5 52 4210 5
Size: 47 4158 2081
Fragments arranged by size:
4158 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6286
StyI C'CwwG_G
Cuts at: 513 1065 2284 3725 513
Size: 552 1219 1441 3074
Fragments arranged by size:
3074 1441 1219 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2450 2562 4313 4330 4483 4668 5113
Size: 1255 870 112 1751 17 153 185 445
Cuts at: 5113 325
Size: 1498
Fragments arranged by size:
1751 1498 1255 870 445 185 153 112
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2439 2703 4532
Size: 274 80 33 51 1443 462 264 1829
Cuts at: 4532 6244 96
Size: 1712 138
Fragments arranged by size:
1829 1712 1443 462 274 264 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6246 6278 6246
Size: 32 6254
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6286
XbaI T'CTAG_A
Cuts at: 3622 3622
Size: 6286
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6286
XmaI C'CCGG_G
Cuts at: 1870 3611 1870
Size: 1741 4545
XmnI GAAnn'nnTTC
Cuts at: 4415 4415
Size: 6286
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4094 276
Size: 53 83 186 936 2560 2468
Fragments arranged by size:
2560 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI
PvuI PvuII SacI SalI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII
EcoNI EcoO109I FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI
RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI
XcmI