Restriction map for
pFN21AB9873
(Circular) (Six-base) MAPSORT of: pFN21AB9873.seq Check: 8849 from: 1 to: 6202
pFN21AB9873 6202 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3185 3185
Size: 6202
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4012 278
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3545 1937
Size: 1608 4594
Acc65I G'GTAC_C
Cuts at: 2637 3523 2637
Size: 886 5316
AclI AA'CG_TT
Cuts at: 1563 4329 4702 1563
Size: 2766 373 3063
Fragments arranged by size:
3063 2766 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2355 4265 5649 5917 1632
Size: 723 1910 1384 268 1917
Fragments arranged by size:
1917 1910 1384 723 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6005
AflIII A'CryG_T
Cuts at: 1203 5101 6078 1203
Size: 3898 977 1327
Fragments arranged by size:
3898 1327 977
AgeI A'CCGG_T
Cuts at: 3217 5033 3217
Size: 1816 4386
AhdI GACnn_n'nnGTC
Cuts at: 3306 4931 3306
Size: 1625 4577
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6202
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6202
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3129 3161 3129
Size: 32 6170
AlwNI CAG_nnn'CTG
Cuts at: 2486 2544 3945 5517 2486
Size: 58 1401 1572 3171
Fragments arranged by size:
3171 1572 1401 58
ApaLI G'TGCA_C
Cuts at: 4259 5415 4259
Size: 1156 5046
ApoI r'AATT_y
Cuts at: 1493 2340 2711 3511 3737 3889 5998 1493
Size: 847 371 800 226 152 2109 1697
Fragments arranged by size:
2109 1697 847 800 371 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4756 160
Size: 872 3724 1606
Fragments arranged by size:
3724 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
AvaI C'yCGr_G
Cuts at: 1870 1943 3527 1870
Size: 73 1584 4545
Fragments arranged by size:
4545 1584 73
BamHI G'GATC_C
Cuts at: 3532 3899 3532
Size: 367 5835
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2055 2637 3523 4978 618
Size: 325 217 627 268 582 886 1455 1842
Fragments arranged by size:
1842 1455 886 627 582 325 268 217
BanII G_rGCy'C
Cuts at: 1391 2480 3521 1391
Size: 1089 1041 4072
Fragments arranged by size:
4072 1089 1041
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2589 928
Size: 971 690 4541
Fragments arranged by size:
4541 971 690
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2024 2058 4393 4427 1903
Size: 34 87 34 2335 34 3678
Fragments arranged by size:
3678 2335 87 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4094 5304 4094
Size: 1210 4992
BclI T'GATC_A
Cuts at: 1548 2112 1548
Size: 564 5638
BfrBI ATG'CAT
Cuts at: 2590 2590
Size: 6202
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4813 136
Size: 107 122 71 631 605 3141 1525
Fragments arranged by size:
3141 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6197 6197
Size: 6202
BlpI GC'TnA_GC
Cuts at: 2847 3619 2847
Size: 772 5430
Bme1580I G_kGCm'C
Cuts at: 1790 2058 2319 4263 5419 1790
Size: 268 261 1944 1156 2573
Fragments arranged by size:
2573 1944 1156 268 261
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6202
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2155 3067 3933
Size: 416 53 83 399 24 738 912 866
Cuts at: 3933 4891 442
Size: 958 1753
Fragments arranged by size:
1753 958 912 866 738 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2930 4862 1239
Size: 1691 1932 2579
Fragments arranged by size:
2579 1932 1691
Bpu10I CC'TnA_GC
Cuts at: 2155 2479 2155
Size: 324 5878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4327 5192 5490 4327
Size: 865 298 5039
Fragments arranged by size:
5039 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2016 2132 2165 2948 4865 882
Size: 617 517 116 33 783 1917 2219
Fragments arranged by size:
2219 1917 783 617 517 116 33
BsaAI yAC'GTr
Cuts at: 493 1204 3993 493
Size: 711 2789 2702
Fragments arranged by size:
2789 2702 711
BsaBI GATnn'nnATC
Cuts at: 2117 2525 6196 2117
Size: 408 3671 2123
Fragments arranged by size:
3671 2123 408
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4009 4391 5026 275
Size: 53 83 186 936 2476 382 635 1451
Fragments arranged by size:
2476 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2018 2892 3217 4635 5033 5307 5454 5865 2018
Size: 874 325 1418 398 274 147 411 2355
Fragments arranged by size:
2355 1418 874 411 398 325 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3129 3159 1178
Size: 30 165 30 1726 30 4221
Fragments arranged by size:
4221 1726 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 6202
BseYI C'CCAG_C
Cuts at: 2026 2142 2356 2738 3080 5405 5901 2026
Size: 116 214 382 342 2325 496 2327
Fragments arranged by size:
2327 2325 496 382 342 214 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5629
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2242 3652 4413 5441 1540
Size: 275 186 241 1410 761 1028 2301
Fragments arranged by size:
2301 1410 1028 761 275 241 186
BsiHKAI G_wGCw'C
Cuts at: 2480 4263 4348 5419 2480
Size: 1783 85 1071 3263
Fragments arranged by size:
3263 1783 1071 85
BsmI GAATG_Cn'
Cuts at: 3713 3806 3713
Size: 93 6109
BsmBI CGTCTCn'nnnn_
Cuts at: 3118 3118
Size: 6202
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2058 2319 2480 3521 4263 4348 5419
Size: 399 268 261 161 1041 742 85 1071
Cuts at: 5419 1391
Size: 2174
Fragments arranged by size:
2174 1071 1041 742 399 268 261 161
85
BspEI T'CCGG_A
Cuts at: 5865 5865
Size: 6202
BspHI T'CATG_A
Cuts at: 2766 4091 5821 2766
Size: 1325 1730 3147
Fragments arranged by size:
3147 1730 1325
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3185 3557 844
Size: 2341 372 3489
Fragments arranged by size:
3489 2341 372
BsrBI CCG'CTC
Cuts at: 1394 1823 4089 1394
Size: 429 2266 3507
Fragments arranged by size:
3507 2266 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2555 4697 4871 65
Size: 1183 1307 2142 174 1396
Fragments arranged by size:
2142 1396 1307 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2747 3217 4846 5033 1700
Size: 110 937 470 1629 187 2869
Fragments arranged by size:
2869 1629 937 470 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6202
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6202
BssSI C'ACGA_G
Cuts at: 2090 4262 5274 2090
Size: 2172 1012 3018
Fragments arranged by size:
3018 2172 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6202
BstBI TT'CG_AA
Cuts at: 5006 5006
Size: 6202
BstEII G'GTnAC_C
Cuts at: 1184 2602 1184
Size: 1418 4784
BstXI CCAn_nnnn'nTGG
Cuts at: 2011 2872 2011
Size: 861 5341
BstYI r'GATC_y
Cuts at: 1972 3315 3384 3532 3899 4284 4301 5753 5839
Size: 1343 69 148 367 385 17 1452 86
Cuts at: 5839 5851 6197 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 1343 385 367 346 148 86
69 17 12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5650
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2384 487
Size: 683 56 1158 4305
Fragments arranged by size:
4305 1158 683 56
BtsI GCAGTG_nn'
Cuts at: 3718 4512 4532 3718
Size: 794 20 5388
Fragments arranged by size:
5388 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6202
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6167
DraI TTT'AAA
Cuts at: 3506 3506
Size: 6202
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5209 809
Size: 507 3893 1802
Fragments arranged by size:
3893 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2013 2787 2874
Size: 54 1425 50 275 18 183 774 87
Cuts at: 2874 3649 4538 6167 8
Size: 775 889 1629 43
Fragments arranged by size:
1629 1425 889 775 774 275 183 87
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3649 1537
Size: 275 1837 4090
Fragments arranged by size:
4090 1837 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2406 3014 4132 1353
Size: 1053 608 1118 3423
Fragments arranged by size:
3423 1118 1053 608
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4789 5163 5309 387
Size: 1064 138 3200 374 146 1280
Fragments arranged by size:
3200 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2478 2478
Size: 6202
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2355 2930 4265 4862 5649 5917 1239
Size: 393 723 575 1335 597 787 268 1524
Fragments arranged by size:
1524 1335 787 723 597 575 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2185 2483 2185
Size: 298 5904
EcoRI G'AATT_C
Cuts at: 2711 3511 3889 2711
Size: 800 378 5024
Fragments arranged by size:
5024 800 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2471 2471
Size: 6202
FspI TGC'GCA
Cuts at: 4708 4708
Size: 6202
HaeII r_GCGC'y
Cuts at: 1678 5349 1678
Size: 3671 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2315 2347 2941 2973 3053
Size: 32 135 32 743 32 594 32 80
Cuts at: 3053 3085 3292 3324 4843 4875 4917 4949 1373
Size: 32 207 32 1519 32 42 32 2626
Fragments arranged by size:
2626 1519 743 594 207 135 80 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3204 3492 3546 3792 669
Size: 988 281 1266 288 54 246 3079
Fragments arranged by size:
3079 1266 988 288 281 246 54
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6202
HpaI GTT'AAC
Cuts at: 3204 3492 3792 3204
Size: 288 300 5614
Fragments arranged by size:
5614 300 288
KpnI G_GTAC'C
Cuts at: 2641 3527 2641
Size: 886 5316
MfeI C'AATT_G
Cuts at: 2532 3801 2532
Size: 1269 4933
MluI A'CGCG_T
Cuts at: 6078 6078
Size: 6202
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2646 3173 5316 5500 1305
Size: 1341 527 2143 184 2007
Fragments arranged by size:
2143 2007 1341 527 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2015 2789 2876 10
Size: 54 1425 343 183 774 87 3336
Fragments arranged by size:
3336 1425 774 343 183 87 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 2595 4160 4519 4678 518
Size: 648 843 586 1565 359 159 2042
Fragments arranged by size:
2042 1565 843 648 586 359 159
MspA1I CmG'CkG
Cuts at: 1606 3618 4295 5443 5688 1606
Size: 2012 677 1148 245 2120
Fragments arranged by size:
2120 2012 1148 677 245
NaeI GCC'GGC
Cuts at: 2749 2749
Size: 6202
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5650
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6202
NgoMIV G'CCGG_C
Cuts at: 2747 2747
Size: 6202
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6202
NotI GC'GGCC_GC
Cuts at: 3649 3649
Size: 6202
NsiI A_TGCA'T
Cuts at: 2592 2592
Size: 6202
NspI r_CATG'y
Cuts at: 1132 1229 2590 3560 5105 1132
Size: 97 1361 970 1545 2229
Fragments arranged by size:
2229 1545 1361 970 97
PasI CC'CwG_GG
Cuts at: 1348 2051 2812 1348
Size: 703 761 4738
Fragments arranged by size:
4738 761 703
PciI A'CATG_T
Cuts at: 5101 5101
Size: 6202
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6202
PfoI T'CCnGG_A
Cuts at: 2568 3387 2568
Size: 819 5383
PmeI GTTT'AAAC
Cuts at: 3506 3506
Size: 6202
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3059 3091 3129 3161 4271 4303 5784 5816 3059
Size: 32 38 32 1110 32 1481 32 3445
Fragments arranged by size:
3445 1481 1110 38 32 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 2185 2185
Size: 6202
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6202
PsiI TTA'TAA
Cuts at: 3772 3772
Size: 6202
PstI C_TGCA'G
Cuts at: 830 3554 830
Size: 2724 3478
PvuI CG_AT'CG
Cuts at: 2001 2242 2001
Size: 241 5961
SacI G_AGCT'C
Cuts at: 2480 2480
Size: 6202
SalI G'TCGA_C
Cuts at: 1936 3544 1936
Size: 1608 4594
SanDI GG'GwC_CC
Cuts at: 2185 2185
Size: 6202
SapI GCTCTTCn'nnn_
Cuts at: 2406 2406
Size: 6202
SbfI CC_TGCA'GG
Cuts at: 3554 3554
Size: 6202
ScaI AGT'ACT
Cuts at: 2231 4450 6162 2231
Size: 2219 1712 2271
Fragments arranged by size:
2271 2219 1712
SfcI C'TryA_G
Cuts at: 826 1045 3126 3550 4685 5366 5557 826
Size: 219 2081 424 1135 681 191 1471
Fragments arranged by size:
2081 1471 1135 681 424 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6202
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6202
SmaI CCC'GGG
Cuts at: 1872 3529 1872
Size: 1657 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4306 5207 5469 820
Size: 197 926 2363 901 262 1553
Fragments arranged by size:
2363 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3993 493
Size: 3500 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6202
SphI G_CATG'C
Cuts at: 1132 3560 1132
Size: 2428 3774
SspI AAT'ATT
Cuts at: 5 52 3478 4126 5
Size: 47 3426 648 2081
Fragments arranged by size:
3426 2081 648 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6202
StyI C'CwwG_G
Cuts at: 513 1065 3453 3641 513
Size: 552 2388 188 3074
Fragments arranged by size:
3074 2388 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2073 4229 4246 4399 4584 5029 325
Size: 1255 493 2156 17 153 185 445 1498
Fragments arranged by size:
2156 1498 1255 493 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2229 2271 4448
Size: 274 80 33 51 1443 252 42 2177
Cuts at: 4448 6160 96
Size: 1712 138
Fragments arranged by size:
2177 1712 1443 274 252 138 80 51
42 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2603 2635 2862 2894 3156 3188 6162 6194 2603
Size: 32 227 32 262 32 2974 32 2611
Fragments arranged by size:
2974 2611 262 227 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6202
XbaI T'CTAG_A
Cuts at: 3538 3538
Size: 6202
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3096 3451 3096
Size: 355 5847
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6202
XmaI C'CCGG_G
Cuts at: 1870 3527 1870
Size: 1657 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 4331 2340
Size: 1991 4211
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4010 276
Size: 53 83 186 936 2476 2468
Fragments arranged by size:
2476 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI
NdeI NgoMIV NheI NotI NsiI NspI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI
PvuI SacI SalI SanDI SapI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BbvCI BplI
BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI
KasI NarI NruI PacI PmlI PspOMI PspXI PsrI
PvuII RsrII SacII SexAI SfiI SfoI SrfI SwaI