Restriction map for
pFN21AB9872
(Circular) (Six-base) MAPSORT of: pFN21AB9872.seq Check: 9464 from: 1 to: 6190
pFN21AB9872 6190 bp
With 182 enzymes: SgfI *
February 27, 2009 14:45 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4000 278
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3533 1937
Size: 1596 4594
Acc65I G'GTAC_C
Cuts at: 3511 3511
Size: 6190
AclI AA'CG_TT
Cuts at: 1563 4317 4690 1563
Size: 2754 373 3063
Fragments arranged by size:
3063 2754 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2600 4253 5637 5905 1632
Size: 968 1653 1384 268 1917
Fragments arranged by size:
1917 1653 1384 968 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5993
AflIII A'CryG_T
Cuts at: 1203 2337 3135 5089 6066 1203
Size: 1134 798 1954 977 1327
Fragments arranged by size:
1954 1327 1134 977 798
AgeI A'CCGG_T
Cuts at: 5021 5021
Size: 6190
AhdI GACnn_n'nnGTC
Cuts at: 4919 4919
Size: 6190
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6190
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6190
AlwNI CAG_nnn'CTG
Cuts at: 2947 3933 5505 2947
Size: 986 1572 3632
Fragments arranged by size:
3632 1572 986
ApaI G_GGCC'C
Cuts at: 2499 2499
Size: 6190
ApaLI G'TGCA_C
Cuts at: 4247 5403 4247
Size: 1156 5034
ApoI r'AATT_y
Cuts at: 1493 3499 3725 3877 5986 1493
Size: 2006 226 152 2109 1697
Fragments arranged by size:
2109 2006 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4744 160
Size: 872 3712 1606
Fragments arranged by size:
3712 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
AvaI C'yCGr_G
Cuts at: 1870 1943 2050 2438 2483 2491 2573 3515 1870
Size: 73 107 388 45 8 82 942 4545
Fragments arranged by size:
4545 942 388 107 82 73 45 8
BamHI G'GATC_C
Cuts at: 3520 3887 3520
Size: 367 5823
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2603 2813 3511 4966 618
Size: 325 217 627 816 210 698 1455 1842
Fragments arranged by size:
1842 1455 816 698 627 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2492 2499 3173 3509 1391
Size: 1101 7 674 336 4072
Fragments arranged by size:
4072 1101 674 336 7
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5219
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4381 4415 1903
Size: 34 2444 34 3678
Fragments arranged by size:
3678 2444 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3089 3119 4082 5292 3089
Size: 30 963 1210 3987
Fragments arranged by size:
3987 1210 963 30
BclI T'GATC_A
Cuts at: 1548 2124 1548
Size: 576 5614
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4801 136
Size: 107 122 71 631 605 3129 1525
Fragments arranged by size:
3129 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6185 6185
Size: 6190
BlpI GC'TnA_GC
Cuts at: 2826 3607 2826
Size: 781 5409
Bme1580I G_kGCm'C
Cuts at: 1790 2331 2499 2606 2816 4251 5407 1790
Size: 541 168 107 210 1435 1156 2573
Fragments arranged by size:
2573 1435 1156 541 210 168 107
BmgBI CAC'GTC
Cuts at: 1318 3292 1318
Size: 1974 4216
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3921 4879 442
Size: 416 53 83 399 24 2504 958 1753
Fragments arranged by size:
2504 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6190
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2582 2924 3058 4850 1239
Size: 1343 342 134 1792 2579
Fragments arranged by size:
2579 1792 1343 342 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 4315 5180 5478 2144
Size: 2171 865 298 2856
Fragments arranged by size:
2856 2171 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4853 882
Size: 617 3354 2219
Fragments arranged by size:
3354 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3981 493
Size: 711 2777 2702
Fragments arranged by size:
2777 2702 711
BsaBI GATnn'nnATC
Cuts at: 2129 6184 2129
Size: 4055 2135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2274 3997 4379 5014
Size: 53 83 186 936 741 1723 382 635
Cuts at: 5014 275
Size: 1451
Fragments arranged by size:
1723 1451 936 741 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4623 5021 5295 5442 5853 4623
Size: 398 274 147 411 4960
Fragments arranged by size:
4960 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2327 2342 2357 2372 1178
Size: 30 165 30 924 15 15 15 4996
Fragments arranged by size:
4996 924 165 30 30 15 15 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2363 2390 2435 2438 1186
Size: 1177 27 45 3 4938
Fragments arranged by size:
4938 1177 45 27 3
BseYI C'CCAG_C
Cuts at: 2191 2264 2735 2809 5393 5889 2191
Size: 73 471 74 2584 496 2492
Fragments arranged by size:
2584 2492 496 471 74 73
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3051 1163
Size: 573 1315 4302
Fragments arranged by size:
4302 1315 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2046 2254 2305 3640 4401 5429
Size: 275 186 45 208 51 1335 761 1028
Cuts at: 5429 1540
Size: 2301
Fragments arranged by size:
2301 1335 1028 761 275 208 186 51
45
BsiHKAI G_wGCw'C
Cuts at: 2492 4251 4336 5407 2492
Size: 1759 85 1071 3275
Fragments arranged by size:
3275 1759 1071 85
BsmI GAATG_Cn'
Cuts at: 3701 3794 3701
Size: 93 6097
BsmBI CGTCTCn'nnnn_
Cuts at: 2144 2524 2558 2144
Size: 380 34 5776
Fragments arranged by size:
5776 380 34
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2331 2492 2499 2606 2816 3173 3509
Size: 399 541 161 7 107 210 357 336
Cuts at: 3509 4251 4336 5407 1391
Size: 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 541 399 357 336 210
161 107 85 7
BspEI T'CCGG_A
Cuts at: 5853 5853
Size: 6190
BspHI T'CATG_A
Cuts at: 2763 4079 5809 2763
Size: 1316 1730 3144
Fragments arranged by size:
3144 1730 1316
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3545 844
Size: 2701 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4077 1394
Size: 429 2254 3507
Fragments arranged by size:
3507 2254 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4685 4859 65
Size: 1183 3437 174 1396
Fragments arranged by size:
3437 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2259 2317 4834 5021 1700
Size: 110 449 58 2517 187 2869
Fragments arranged by size:
2869 2517 449 187 110 58
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6190
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6190
BssSI C'ACGA_G
Cuts at: 2102 4250 5262 2102
Size: 2148 1012 3030
Fragments arranged by size:
3030 2148 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6190
BstBI TT'CG_AA
Cuts at: 4994 4994
Size: 6190
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6190
BstXI CCAn_nnnn'nTGG
Cuts at: 2866 2866
Size: 6190
BstYI r'GATC_y
Cuts at: 1972 2943 3520 3887 4272 4289 5741 5827 5839
Size: 971 577 367 385 17 1452 86 12
Cuts at: 5839 6185 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 971 577 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2231 2876 2231
Size: 645 5545
BtgI C'CryG_G
Cuts at: 513 1065 2382 2628 513
Size: 552 1317 246 4075
Fragments arranged by size:
4075 1317 552 246
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2494 3169 487
Size: 683 56 1268 675 3508
Fragments arranged by size:
3508 1268 683 675 56
BtsI GCAGTG_nn'
Cuts at: 3039 3441 3706 4500 4520 3039
Size: 402 265 794 20 4709
Fragments arranged by size:
4709 794 402 265 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6190
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6155
DraI TTT'AAA
Cuts at: 3494 3494
Size: 6190
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2014 5197 809
Size: 507 698 3183 1802
Fragments arranged by size:
3183 1802 698 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2302 2784
Size: 54 1425 50 275 18 213 259 482
Cuts at: 2784 2868 3637 4526 6155 8
Size: 84 769 889 1629 43
Fragments arranged by size:
1629 1425 889 769 482 275 259 213
84 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2043 2302 3637 1537
Size: 275 231 259 1335 4090
Fragments arranged by size:
4090 1335 275 259 231
EarI CTCTTCn'nnn_
Cuts at: 1353 2477 3008 3264 3372 4120 1353
Size: 1124 531 256 108 748 3423
Fragments arranged by size:
3423 1124 748 531 256 108
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3065 4777 5151 5297 387
Size: 1064 138 1476 1712 374 146 1280
Fragments arranged by size:
1712 1476 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2490 2490
Size: 6190
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2582 2600 2924 3058 4253 4850 5637
Size: 393 950 18 324 134 1195 597 787
Cuts at: 5637 5905 1239
Size: 268 1524
Fragments arranged by size:
1524 1195 950 787 597 393 324 268
134 18
EcoO109I rG'GnC_Cy
Cuts at: 2495 2935 2495
Size: 440 5750
EcoRI G'AATT_C
Cuts at: 3499 3877 3499
Size: 378 5812
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2764 2764
Size: 6190
FspI TGC'GCA
Cuts at: 4696 4696
Size: 6190
HaeII r_GCGC'y
Cuts at: 1678 2431 2458 5337 1678
Size: 753 27 2879 2531
Fragments arranged by size:
2879 2531 753 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2327 2359 3147 3179 4831
Size: 32 135 32 755 32 788 32 1652
Cuts at: 4831 4863 4905 4937 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1652 788 755 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2323 3534 3780 669
Size: 988 281 385 1211 246 3079
Fragments arranged by size:
3079 1211 988 385 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6190
HpaI GTT'AAC
Cuts at: 3780 3780
Size: 6190
KpnI G_GTAC'C
Cuts at: 3515 3515
Size: 6190
MfeI C'AATT_G
Cuts at: 3150 3201 3433 3789 3150
Size: 51 232 356 5551
Fragments arranged by size:
5551 356 232 51
MluI A'CGCG_T
Cuts at: 6066 6066
Size: 6190
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2670 5304 5488 1305
Size: 1365 2634 184 2007
Fragments arranged by size:
2634 2007 1365 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2786 2870 10
Size: 54 1425 343 954 84 3330
Fragments arranged by size:
3330 1425 954 343 84 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 4148 4507 4666 518
Size: 648 843 2139 359 159 2042
Fragments arranged by size:
2139 2042 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3606 4283 5431 5676 1606
Size: 2000 677 1148 245 2120
Fragments arranged by size:
2120 2000 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2382 513
Size: 552 1317 4321
Fragments arranged by size:
4321 1317 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6190
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6190
NotI GC'GGCC_GC
Cuts at: 2302 3637 2302
Size: 1335 4855
NspI r_CATG'y
Cuts at: 1132 1229 2215 2341 3139 3548 5093 1132
Size: 97 986 126 798 409 1545 2229
Fragments arranged by size:
2229 1545 986 798 409 126 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6190
PciI A'CATG_T
Cuts at: 2337 3135 5089 2337
Size: 798 1954 3438
Fragments arranged by size:
3438 1954 798
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2947 1108
Size: 1839 4351
PfoI T'CCnGG_A
Cuts at: 2394 2394
Size: 6190
PmeI GTTT'AAAC
Cuts at: 3494 3494
Size: 6190
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4259 4291 5772 5804 4259
Size: 32 1481 32 4645
Fragments arranged by size:
4645 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3327 1613
Size: 1714 4476
PsiI TTA'TAA
Cuts at: 3760 3760
Size: 6190
PspOMI G'GGCC_C
Cuts at: 2495 2495
Size: 6190
PstI C_TGCA'G
Cuts at: 830 3542 830
Size: 2712 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6190
SacI G_AGCT'C
Cuts at: 2492 2492
Size: 6190
SalI G'TCGA_C
Cuts at: 1936 3532 1936
Size: 1596 4594
SapI GCTCTTCn'nnn_
Cuts at: 2477 3264 2477
Size: 787 5403
SbfI CC_TGCA'GG
Cuts at: 3542 3542
Size: 6190
ScaI AGT'ACT
Cuts at: 2243 4438 6150 2243
Size: 2195 1712 2283
Fragments arranged by size:
2283 2195 1712
SfcI C'TryA_G
Cuts at: 826 1045 3120 3538 4673 5354 5545 826
Size: 219 2075 418 1135 681 191 1471
Fragments arranged by size:
2075 1471 1135 681 418 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6190
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6190
SmaI CCC'GGG
Cuts at: 1872 2052 3517 1872
Size: 180 1465 4545
Fragments arranged by size:
4545 1465 180
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2159 4294 5195 5457 820
Size: 197 926 216 2135 901 262 1553
Fragments arranged by size:
2135 1553 926 901 262 216 197
SnaBI TAC'GTA
Cuts at: 493 3981 493
Size: 3488 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6190
SphI G_CATG'C
Cuts at: 1132 2215 3548 1132
Size: 1083 1333 3774
Fragments arranged by size:
3774 1333 1083
SspI AAT'ATT
Cuts at: 5 52 4114 5
Size: 47 4062 2081
Fragments arranged by size:
4062 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6190
StyI C'CwwG_G
Cuts at: 513 1065 2382 3629 513
Size: 552 1317 1247 3074
Fragments arranged by size:
3074 1317 1247 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3497 4217 4234 4387 4572 5017 325
Size: 1255 1917 720 17 153 185 445 1498
Fragments arranged by size:
1917 1498 1255 720 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2241 3036 3363
Size: 274 80 33 51 1443 264 795 327
Cuts at: 3363 4436 6148 96
Size: 1073 1712 138
Fragments arranged by size:
1712 1443 1073 795 327 274 264 138
80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2669 2701 3281 3313 6150 6182 2669
Size: 32 580 32 2837 32 2677
Fragments arranged by size:
2837 2677 580 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6190
XbaI T'CTAG_A
Cuts at: 3526 3526
Size: 6190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2063 2188 2063
Size: 125 6065
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6190
XmaI C'CCGG_G
Cuts at: 1870 2050 3515 1870
Size: 180 1465 4545
Fragments arranged by size:
4545 1465 180
XmnI GAAnn'nnTTC
Cuts at: 4319 4319
Size: 6190
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3998 276
Size: 53 83 186 936 2464 2468
Fragments arranged by size:
2468 2464 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PfoI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI SacI SalI SapI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV
FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI
PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII
SanDI SexAI SfiI SfoI SrfI SwaI